RLG00000032485

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003915
Physical Location & Seq
Forward (+)
15764993 .. 15767737
2745 bp
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UTR
Exon/CDS
Intron
RLM00000032485

Sequence Viewer

Length: 528 bp
ATGGCTATGACTAAACCTCATTGGCAAGCAGAGAAAGAAGAAATCATAAAGCAGGGAGATAACTACTTTATCAAACCACGAGGACTTAATATTGTATGGGGCAATGATGAACGGTACTGGAAAATACCAGCCAGAGGGACTGAGGATCCTGCAGAGCTGGTACAGGTCTCGTGGCTGGAAGTCAAAGGCTCAGTTGAGTTGATAGCCGGGGAGGGGTACGAACTGACCTTCGATGTGGGACTGGCAGAAGATGCATTTGGTTGGAAAGACATACAAGTGTTCTTGATGGCAAAGGTAGGGAAAAAGGGAAAGTACAAATGGACAAGAGTTAAACTCGATCAGGATCAGAATAACGAGAGAGTTACGATTCCTGATCAACAGAAGCTTAGCATTGATGTTCCAGCTGATACAATGGACACCGACCTTCATTTTGGTTTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATTTATAATGCGAAAGTAACCTTGGATTTGCCACCTACCGCACCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

176

Amino Acids

19.98

Weight (kDa)

5.3

Isoelectric Point (pI)

13.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 21 - 162 1.1e-20 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
AciI CCGC 1 cut(s) 516
AclWI GGATC 3 cut(s) 140, 153, 351
AfaI GTAC 4 cut(s) 116, 162, 218, 314
AfiI CCNNNNNNNGG 2 cut(s) 134, 213
AgsI TTSAA 1 cut(s) 475
AjuI GAANNNNNNNTTGG 4 cut(s) 240, 272, 482, 514
AluBI AGCT 3 cut(s) 157, 385, 404
AluI AGCT 3 cut(s) 157, 385, 404
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 3 cut(s) 140, 153, 351
ArsI GACNNNNNNTTYG 2 cut(s) 413, 445
AsuC2I CCSGG 1 cut(s) 208
BamHI GGATCC 1 cut(s) 145
BauI CACGAG 2 cut(s) 78, 169
BccI CCATC 1 cut(s) 280
BclI TGATCA 1 cut(s) 373
BcnI CCSGG 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 172
BfmI CTRYAG 1 cut(s) 150
BlpI GCTNAGC 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 208
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 318, 350
Bpu1102I GCTNAGC 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 172
BsaJI CCNNGG 2 cut(s) 207, 498
BsaXI ACNNNNNCTCC 1 cut(s) 505
Bsc4I CCNNNNNNNGG 2 cut(s) 134, 213
Bse1I ACTGG 2 cut(s) 122, 246
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 207, 498
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 134, 213
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 132, 204
BseNI ACTGG 2 cut(s) 122, 246
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 2 cut(s) 151, 252
BslI CCNNNNNNNGG 2 cut(s) 134, 213
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 2 cut(s) 151, 252
Bso31I GGTCTC 1 cut(s) 172
Bsp143I GATC 4 cut(s) 145, 337, 343, 373
Bsp1720I GCTNAGC 1 cut(s) 386
BspACI CCGC 1 cut(s) 516
BspCNI CTCAG 2 cut(s) 133, 203
BspLI GGNNCC 1 cut(s) 147
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 3 cut(s) 140, 153, 351
BspTNI GGTCTC 1 cut(s) 172
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 2 cut(s) 122, 246
BssECI CCNNGG 2 cut(s) 207, 498
BssMI GATC 4 cut(s) 145, 337, 343, 373
BssSI CACGAG 2 cut(s) 78, 169
BssT1I CCWWGG 1 cut(s) 498
Bst2BI CACGAG 2 cut(s) 78, 169
Bst4CI ACNGT 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 251
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 3 cut(s) 141, 190, 386
BstKTI GATC 4 cut(s) 148, 340, 346, 376
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 4 cut(s) 145, 337, 343, 373
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 27
Csp6I GTAC 4 cut(s) 115, 161, 217, 313
CviJI RGCY 9 cut(s) 5, 131, 157, 175, 189, 206, 385, 404, 471
CviKI_1 RGCY 9 cut(s) 5, 131, 157, 175, 189, 206, 385, 404, 471
CviQI GTAC 4 cut(s) 115, 161, 217, 313
DdeI CTNAG 3 cut(s) 141, 190, 386
DpnI GATC 4 cut(s) 147, 339, 345, 375
DpnII GATC 4 cut(s) 145, 337, 343, 373
Eco130I CCWWGG 1 cut(s) 498
Eco31I GGTCTC 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 498
FaiI YATR 6 cut(s) 8, 47, 97, 272, 441, 483
FaqI GGGAC 2 cut(s) 151, 252
FbaI TGATCA 1 cut(s) 373
HapII CCGG 1 cut(s) 207
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 1 cut(s) 367
HpaII CCGG 1 cut(s) 207
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 3 cut(s) 283, 341, 371
HpyAV CCTTC 2 cut(s) 238, 434
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4V TGCA 2 cut(s) 152, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 3 cut(s) 141, 190, 386
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 145, 337, 343, 373
LweI GCATC 1 cut(s) 241
MaeIII GTNAC 2 cut(s) 361, 493
MalI GATC 4 cut(s) 147, 339, 345, 375
MboI GATC 4 cut(s) 145, 337, 343, 373
MboII GAAGA 3 cut(s) 50, 260, 487
MflI RGATCY 1 cut(s) 145
MmeI TCCRAC 1 cut(s) 242
MnlI CCTC 6 cut(s) 27, 74, 128, 136, 205, 461
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 2 cut(s) 87, 330
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 208
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 1 cut(s) 208
NdeII GATC 4 cut(s) 145, 337, 343, 373
NlaIV GGNNCC 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 256
PfeI GAWTC 1 cut(s) 367
PsiI TTATAA 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 147
PstI CTGCAG 1 cut(s) 154
PsuI RGATCY 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 4 cut(s) 116, 162, 218, 314
RsaNI GTAC 4 cut(s) 115, 161, 217, 313
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 2 cut(s) 87, 330
Sau3AI GATC 4 cut(s) 145, 337, 343, 373
ScrFI CCNGG 1 cut(s) 208
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 275
SsiI CCGC 1 cut(s) 516
SspI AATATT 1 cut(s) 91
StyD4I CCNGG 1 cut(s) 206
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 114
TaqI TCGA 2 cut(s) 231, 336
TatI WGTACW 1 cut(s) 312
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 2 cut(s) 87, 330
Tru9I TTAA 2 cut(s) 87, 330
TspDTI ATGAA 3 cut(s) 123, 416, 456
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.