Rorug05G0055400

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0003915
Physical Location & Seq
Reverse (-)
4886812 .. 4890803
3992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0055400.1

Sequence Viewer

Length: 714 bp
ATGACTGTAATATCCTCAACAAACAAGAGTAATATTGGTTCAGACCAGGAAAGCTGTGTCGTTGTCTATTCGCATTTCGCAGAGACTCGGGCTTCTTCTGGGACCTTTCATATACCAAGTCAGAAGCCAACTGACGCCAACCAAGTCAGGACCAAATTTTGTGATGCATTGCAGCACCTCTCTTATGACTTCAGTCGGCTGTTGCTGTATCGATTTTATCTTCTCCTCTACTACTCACCAATCCTCAAAAGAAGTAAAGAACAACAACCCGAAAACAAAGACAAACAGGAAGGATTGACGACGAAAACGAGCCTGGGTTTTAACCTTTCAACGATTCCAAGGTCCATCTTTTTTGGAGATTCTGAAGGAAAAGGATTTGATTCTGGAAACAGTGTGTTATTCAATTTCCCAACTGCATTGGATTCAGTGATGGCAGCAAAACCTGAAGAAACAGAACTAAGGAGACATTTACGTCATCAGAATGGAGAAGGCCAAGCTTTTGGTCCTATTTCTGATATGGAGAAAGATCGACAAATTAGATCAAGAAGGTGGAGACAACGTTCTATGCAAACGATTATTTGTATTGTTCAGTTGCTGCTTTTCTTGGTCTGTATTGAGATTGGTCATTGTCTAGATATCAGTAACAGTGTGCAATCGGAATTTGAAAGTGCTTTGAACTTGGATATTGTGCTTCTGTTCATATTTTACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

237

Amino Acids

27.04

Weight (kDa)

7.0

Isoelectric Point (pI)

63.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 471
AclI AACGTT 1 cut(s) 559
AcsI RAATTY 2 cut(s) 155, 659
AcuI CTGAAG 3 cut(s) 175, 384, 465
AcyI GRCGYC 1 cut(s) 135
AgsI TTSAA 4 cut(s) 330, 403, 665, 676
AjnI CCWGG 2 cut(s) 45, 312
AloI GAACNNNNNNTCC 2 cut(s) 544, 576
AluBI AGCT 2 cut(s) 54, 497
AluI AGCT 2 cut(s) 54, 497
Alw26I GTCTC 3 cut(s) 77, 457, 547
AlwNI CAGNNNCTG 1 cut(s) 595
Ama87I CYCGRG 1 cut(s) 87
AoxI GGCC 1 cut(s) 490
ApeKI GCWGC 3 cut(s) 172, 434, 595
ApoI RAATTY 2 cut(s) 155, 659
AspS9I GGNCC 4 cut(s) 102, 150, 342, 503
AsuHPI GGTGA 1 cut(s) 228
AvaI CYCGRG 1 cut(s) 87
AvaII GGWCC 4 cut(s) 102, 150, 342, 503
BbvI GCAGC 3 cut(s) 184, 446, 582
BccI CCATC 2 cut(s) 353, 424
BciT130I CCWGG 2 cut(s) 47, 314
BcoDI GTCTC 3 cut(s) 77, 457, 547
BfaI CTAG 1 cut(s) 632
BisI GCNGC 3 cut(s) 173, 435, 596
BlsI GCNGC 3 cut(s) 174, 436, 597
Bme1390I CCNGG 2 cut(s) 47, 314
Bme18I GGWCC 4 cut(s) 102, 150, 342, 503
BmeT110I CYCGRG 1 cut(s) 87
BmgT120I GGNCC 4 cut(s) 102, 150, 342, 503
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 2 cut(s) 47, 314
BmsI GCATC 1 cut(s) 154
BoxI GACNNNNGTC 1 cut(s) 192
Bsa29I ATCGAT 1 cut(s) 211
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 2 cut(s) 313, 338
BsaXI ACNNNNNCTCC 2 cut(s) 544, 574
Bse3DI GCAATG 1 cut(s) 167
BseBI CCWGG 2 cut(s) 47, 314
BseCI ATCGAT 1 cut(s) 211
BseDI CCNNGG 2 cut(s) 313, 338
BseMI GCAATG 1 cut(s) 167
BseRI GAGGAG 1 cut(s) 215
BseXI GCAGC 3 cut(s) 184, 446, 582
BshFI GGCC 1 cut(s) 492
BshVI ATCGAT 1 cut(s) 211
BsiHKCI CYCGRG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 115
BsmAI GTCTC 3 cut(s) 77, 457, 547
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 1 cut(s) 492
BsoBI CYCGRG 1 cut(s) 87
Bsp143I GATC 2 cut(s) 526, 539
BspANI GGCC 1 cut(s) 492
BspDI ATCGAT 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 103
BsrDI GCAATG 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 313, 338
BssMI GATC 2 cut(s) 526, 539
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 2 cut(s) 47, 314
Bst4CI ACNGT 3 cut(s) 7, 392, 647
BstACI GRCGYC 1 cut(s) 135
BstDEI CTNAG 1 cut(s) 458
BstKTI GATC 2 cut(s) 529, 542
BstMAI GTCTC 3 cut(s) 77, 457, 547
BstMBI GATC 2 cut(s) 526, 539
BstNI CCWGG 2 cut(s) 47, 314
BstPAI GACNNNNGTC 1 cut(s) 192
BstSCI CCNGG 2 cut(s) 45, 312
BstV1I GCAGC 3 cut(s) 184, 446, 582
BstXI CCANNNNNNTGG 1 cut(s) 500
Bsu15I ATCGAT 1 cut(s) 211
BsuRI GGCC 1 cut(s) 492
BsuTUI ATCGAT 1 cut(s) 211
BtsIMutI CAGTG 3 cut(s) 397, 432, 652
CaiI CAGNNNCTG 1 cut(s) 595
Cfr13I GGNCC 4 cut(s) 102, 150, 342, 503
ClaI ATCGAT 1 cut(s) 211
CseI GACGC 1 cut(s) 143
CviJI RGCY 7 cut(s) 54, 92, 127, 199, 312, 492, 497
CviKI_1 RGCY 7 cut(s) 54, 92, 127, 199, 312, 492, 497
DdeI CTNAG 1 cut(s) 458
DpnI GATC 2 cut(s) 528, 541
DpnII GATC 2 cut(s) 526, 539
DrdI GACNNNNNNGTC 1 cut(s) 471
DseDI GACNNNNNNGTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 338
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 4 cut(s) 102, 150, 342, 503
Eco57I CTGAAG 3 cut(s) 175, 384, 465
Eco88I CYCGRG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 102
EcoRII CCWGG 2 cut(s) 45, 312
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 338
FaiI YATR 6 cut(s) 111, 113, 186, 518, 566, 701
FaqI GGGAC 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 173, 435, 596
Fsp4HI GCNGC 3 cut(s) 173, 435, 596
FspBI CTAG 1 cut(s) 632
GluI GCNGC 3 cut(s) 173, 435, 596
HaeIII GGCC 1 cut(s) 492
HgaI GACGC 1 cut(s) 143
Hin1I GRCGYC 1 cut(s) 135
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 5 cut(s) 85, 334, 359, 380, 422
HphI GGTGA 1 cut(s) 228
Hpy188I TCNGA 6 cut(s) 43, 123, 364, 480, 514, 658
Hpy188III TCNNGA 4 cut(s) 148, 384, 543, 632
Hpy99I CGWCG 1 cut(s) 304
HpyAV CCTTC 4 cut(s) 284, 359, 482, 540
HpyCH4III ACNGT 3 cut(s) 7, 392, 647
HpyCH4IV ACGT 2 cut(s) 472, 559
HpyCH4V TGCA 5 cut(s) 167, 172, 416, 568, 652
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 2 cut(s) 472, 559
Hsp92I GRCGYC 1 cut(s) 135
Kzo9I GATC 2 cut(s) 526, 539
LpnPI CCDG 9 cut(s) 32, 59, 84, 133, 272, 299, 326, 369, 456
Lsp1109I GCAGC 3 cut(s) 184, 446, 582
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 632
MaeII ACGT 2 cut(s) 472, 559
MaeIII GTNAC 1 cut(s) 641
MalI GATC 2 cut(s) 528, 541
MboI GATC 2 cut(s) 526, 539
MboII GAAGA 3 cut(s) 87, 212, 458
MluCI AATT 4 cut(s) 155, 403, 534, 659
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 4 cut(s) 25, 188, 236, 254
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 2 cut(s) 321, 712
MslI CAYNNNNRTG 1 cut(s) 480
MspR9I CCNGG 2 cut(s) 47, 314
MvaI CCWGG 2 cut(s) 47, 314
NdeII GATC 2 cut(s) 526, 539
NlaIV GGNNCC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 305
PfeI GAWTC 4 cut(s) 334, 359, 380, 422
PkrI GCNGC 3 cut(s) 174, 436, 597
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 102
PshAI GACNNNNGTC 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 559
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 45, 312
PspGI CCWGG 2 cut(s) 45, 312
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 4 cut(s) 102, 150, 342, 503
PspPPI RGGWCCY 1 cut(s) 102
PsrI GAACNNNNNNTAC 2 cut(s) 22, 54
PstNI CAGNNNCTG 1 cut(s) 595
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 2 cut(s) 321, 712
SatI GCNGC 3 cut(s) 173, 435, 596
Sau3AI GATC 2 cut(s) 526, 539
Sau96I GGNCC 4 cut(s) 102, 150, 342, 503
SchI GAGTC 1 cut(s) 79
ScrFI CCNGG 2 cut(s) 47, 314
SfaNI GCATC 1 cut(s) 154
SinI GGWCC 4 cut(s) 102, 150, 342, 503
SmiMI CAYNNNNRTG 1 cut(s) 480
Sse9I AATT 4 cut(s) 155, 403, 534, 659
SspI AATATT 1 cut(s) 34
SspMI CTAG 1 cut(s) 632
StyD4I CCNGG 2 cut(s) 45, 312
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 3 cut(s) 7, 392, 647
TaiI ACGT 2 cut(s) 475, 562
TaqI TCGA 2 cut(s) 211, 529
TasI AATT 4 cut(s) 155, 403, 534, 659
TfiI GAWTC 4 cut(s) 334, 359, 380, 422
Tru1I TTAA 2 cut(s) 321, 712
Tru9I TTAA 2 cut(s) 321, 712
TscAI CASTG 3 cut(s) 397, 432, 652
TseI GCWGC 3 cut(s) 172, 434, 595
TspDTI ATGAA 2 cut(s) 98, 688
TspRI CASTG 3 cut(s) 397, 432, 652
VpaK11BI GGWCC 4 cut(s) 102, 150, 342, 503
XapI RAATTY 2 cut(s) 155, 659
XbaI TCTAGA 1 cut(s) 631
XspI CTAG 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.