RchiOBHm_Chr5g0019931

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003915
Physical Location & Seq
Forward (+)
14330213 .. 14331702
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30005

Sequence Viewer

Length: 531 bp
ATGTCTATGACTAAACCTCATTTCCAAGCAGAGAAAGATGAAGATGCAGTCCAAAAGATTGGAGATGGCTACAATATCAAACCACGAGGACTTAACATTGTATGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAGGGCCTGCAGGTGTCGATGTTGCTGCAGAGCTGGTGCAGGTTTCATGGCTGGAAGTAACAGGCTCAGTTGTTTTAACACCTGGGAAGACTTATAAACTGAACTTTGAGGTACAACTGCTATCAGAGGCATTTGGCTGGAAAGATATACAAGCTTTCTTGATGGCCAAGGTAGGGAAAAAGGGAAGATACACGTGGAAAAGGGTTCAAGTACAGCAGCAGGCTGGGACAGGAAACTTCTTTATTCCTAAGGAAGAAGATGAGCTCCGCATTGAAGTTCCACCTGGGAATACTGACAACACCCTTCAGTTCGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATCTATAATGCGAAAGTAACTGCGATTAATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

176

Amino Acids

19.79

Weight (kDa)

8.96

Isoelectric Point (pI)

15.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 24 - 169 8.3e-22 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AarI CACCTGC 1 cut(s) 140
Acc36I ACCTGC 2 cut(s) 140, 169
AciI CCGC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 303
AcuI CTGAAG 1 cut(s) 428
AcvI CACGTG 1 cut(s) 333
AfaI GTAC 2 cut(s) 252, 351
AfiI CCNNNNNNNGG 2 cut(s) 140, 312
AflIII ACRYGT 1 cut(s) 330
AgsI TTSAA 3 cut(s) 347, 413, 493
AjnI CCWGG 2 cut(s) 220, 421
AluBI AGCT 3 cut(s) 172, 293, 403
AluI AGCT 3 cut(s) 172, 293, 403
Alw21I GWGCWC 1 cut(s) 405
AoxI GGCC 2 cut(s) 143, 303
ApeKI GCWGC 2 cut(s) 164, 355
AseI ATTAAT 1 cut(s) 522
AspS9I GGNCC 1 cut(s) 143
AxyI CCTNAGG 1 cut(s) 387
BalI TGGCCA 1 cut(s) 305
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 84
BbrPI CACGTG 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 2 cut(s) 151, 367
BccI CCATC 2 cut(s) 59, 295
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BciT130I CCWGG 2 cut(s) 222, 423
BfmI CTRYAG 2 cut(s) 147, 165
BfuAI ACCTGC 2 cut(s) 140, 169
BglII AGATCT 1 cut(s) 495
BisI GCNGC 2 cut(s) 165, 356
BlsI GCNGC 2 cut(s) 166, 357
Bme1390I CCNGG 2 cut(s) 222, 423
BmgT120I GGNCC 1 cut(s) 143
BmrFI CCNGG 2 cut(s) 222, 423
BmsI GCATC 1 cut(s) 34
BpiI GAAGAC 1 cut(s) 233
BsaAI YACGTR 1 cut(s) 333
BsaJI CCNNGG 3 cut(s) 221, 306, 422
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 312
Bse1I ACTGG 1 cut(s) 128
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 1 cut(s) 115
BseBI CCWGG 2 cut(s) 222, 423
BseDI CCNNGG 3 cut(s) 221, 306, 422
BseLI CCNNNNNNNGG 2 cut(s) 140, 312
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 151, 367
BseYI CCCAGC 1 cut(s) 362
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 2 cut(s) 145, 305
BsiHKAI GWGCWC 1 cut(s) 405
BslFI GGGAC 1 cut(s) 379
BslI CCNNNNNNNGG 2 cut(s) 140, 312
BsmFI GGGAC 1 cut(s) 379
BsnI GGCC 2 cut(s) 145, 305
Bsp1286I GDGCHC 1 cut(s) 405
Bsp143I GATC 1 cut(s) 495
BspACI CCGC 1 cut(s) 406
BspANI GGCC 2 cut(s) 145, 305
BspCNI CTCAG 1 cut(s) 218
BspMAI CTGCAG 2 cut(s) 151, 169
BspMI ACCTGC 2 cut(s) 140, 169
BsrDI GCAATG 1 cut(s) 115
BsrI ACTGG 1 cut(s) 128
BssECI CCNNGG 3 cut(s) 221, 306, 422
BssMI GATC 1 cut(s) 495
BssSI CACGAG 1 cut(s) 84
BssT1I CCWWGG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 84
Bst2UI CCWGG 2 cut(s) 222, 423
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 147, 360
BstDEI CTNAG 2 cut(s) 205, 387
BstKTI GATC 1 cut(s) 498
BstMBI GATC 1 cut(s) 495
BstNI CCWGG 2 cut(s) 222, 423
BstSCI CCNGG 2 cut(s) 220, 421
BstSFI CTRYAG 2 cut(s) 147, 165
BstV1I GCAGC 2 cut(s) 151, 367
BstV2I GAAGAC 1 cut(s) 233
BstX2I RGATCY 1 cut(s) 495
BstXI CCANNNNNNTGG 1 cut(s) 59
BstYI RGATCY 1 cut(s) 495
Bsu36I CCTNAGG 1 cut(s) 387
BsuRI GGCC 2 cut(s) 145, 305
BveI ACCTGC 2 cut(s) 140, 169
Cac8I GCNNGC 2 cut(s) 147, 360
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 251, 350
CviAII CATG 1 cut(s) 186
CviQI GTAC 2 cut(s) 251, 350
DdeI CTNAG 2 cut(s) 205, 387
DpnI GATC 1 cut(s) 497
DpnII GATC 1 cut(s) 495
EaeI YGGCCR 1 cut(s) 303
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 405
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 428
Eco72I CACGTG 1 cut(s) 333
Eco81I CCTNAGG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 403
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 220, 421
EcoT14I CCWWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 405
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 1 cut(s) 189
FaiI YATR 7 cut(s) 8, 103, 187, 234, 287, 459, 501
FaqI GGGAC 1 cut(s) 379
FatI CATG 1 cut(s) 185
Fnu4HI GCNGC 2 cut(s) 165, 356
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 2 cut(s) 165, 356
GluI GCNGC 2 cut(s) 165, 356
GsaI CCCAGC 1 cut(s) 366
HaeIII GGCC 2 cut(s) 145, 305
Hin1II CATG 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 291
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 1 cut(s) 298
HpyAV CCTTC 1 cut(s) 452
HpyCH4IV ACGT 1 cut(s) 332
HpyCH4V TGCA 4 cut(s) 47, 149, 167, 178
HpyF3I CTNAG 2 cut(s) 205, 387
HpySE526I ACGT 1 cut(s) 332
Hsp92II CATG 1 cut(s) 189
Kzo9I GATC 1 cut(s) 495
LmnI GCTCC 1 cut(s) 408
Lsp1109I GCAGC 2 cut(s) 151, 367
LweI GCATC 1 cut(s) 34
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 2 cut(s) 196, 511
MalI GATC 1 cut(s) 497
MboI GATC 1 cut(s) 495
MboII GAAGA 6 cut(s) 53, 238, 336, 404, 407, 505
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 1 cut(s) 405
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MnlI CCTC 5 cut(s) 27, 80, 241, 259, 479
Mox20I TGGCCA 1 cut(s) 305
MscI TGGCCA 1 cut(s) 305
MseI TTAA 3 cut(s) 93, 215, 522
Msp20I TGGCCA 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 222, 423
MvaI CCWGG 2 cut(s) 222, 423
NdeII GATC 1 cut(s) 495
NlaIII CATG 1 cut(s) 189
PaqCI CACCTGC 1 cut(s) 140
PkrI GCNGC 2 cut(s) 166, 357
PmaCI CACGTG 1 cut(s) 333
PmlI CACGTG 1 cut(s) 333
Ppu21I YACGTR 1 cut(s) 333
PshBI ATTAAT 1 cut(s) 522
PsiI TTATAA 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 220, 421
PspCI CACGTG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 362
PspGI CCWGG 2 cut(s) 220, 421
PspPI GGNCC 1 cut(s) 143
PstI CTGCAG 2 cut(s) 151, 169
PsuI RGATCY 1 cut(s) 495
RsaI GTAC 2 cut(s) 252, 351
RsaNI GTAC 2 cut(s) 251, 350
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 3 cut(s) 93, 215, 522
SatI GCNGC 2 cut(s) 165, 356
Sau3AI GATC 1 cut(s) 495
Sau96I GGNCC 1 cut(s) 143
SbfI CCTGCAGG 1 cut(s) 151
ScrFI CCNGG 2 cut(s) 222, 423
SdaI CCTGCAGG 1 cut(s) 151
SduI GDGCHC 1 cut(s) 405
SfaNI GCATC 1 cut(s) 34
SfcI CTRYAG 2 cut(s) 147, 165
Sse8387I CCTGCAGG 1 cut(s) 151
SsiI CCGC 1 cut(s) 406
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 220, 421
StyI CCWWGG 1 cut(s) 306
TaiI ACGT 1 cut(s) 335
TaqI TCGA 1 cut(s) 156
TaqII GACCGA 1 cut(s) 440
TatI WGTACW 1 cut(s) 349
Tru1I TTAA 3 cut(s) 93, 215, 522
Tru9I TTAA 3 cut(s) 93, 215, 522
TseI GCWGC 2 cut(s) 164, 355
TspDTI ATGAA 4 cut(s) 54, 129, 174, 474
VspI ATTAAT 1 cut(s) 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.