Rh5DG144000

Protein PHLOEM PROTEIN 2-LIKE A9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003915
Physical Location & Seq
Forward (+)
14783316 .. 14784456
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG144000.1

Sequence Viewer

Length: 513 bp
ATGGCTATGACTAAACCTCATCACCAAGCAGAGAAAGAAGAAGTCATACCGCTGGGAGAGAACTACCTTATCAAACCACGAGGACTTAATATTGTATGGGGCAATGATGAACGCTACTGGAAAATACCAGCCAAAGGGACTGAGGATCCTGCGGAGCTGGTACAGGTCTCGTGGCTGGAAGTAACAGGCTCAGTTGATGGGTTGAGACCCGGGAAGTATGATCTAACCTTCGAGGTGGCACTGGCAGGAGATGCATTTGGTTGGAAAGACATACAAGTGTTCTTGATGGCAAAGCTAGGGAAAAAGGGAAGGTACAAATGGACAAGAGTTAAACTCGATCAGGATAAGAATAACGTGAAAGTTATAATTCCTGATAACCAGAAGCTTAGCATTGATGTTCCATCTGCTGCAAGTGACACCACCCTTCATTTTGGTCTGTATGAAGTGTGGAGTGGGAAATGGAAAGGAGGCTTGAAGATTTATAATGCGCAAGTAAAAACTGCGACTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

170

Amino Acids

19.15

Weight (kDa)

8.94

Isoelectric Point (pI)

8.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 21 - 163 1.3e-19 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 365, 483
Acc16I TGCGCA 1 cut(s) 489
AciI CCGC 2 cut(s) 50, 152
AclWI GGATC 2 cut(s) 140, 153
AfaI GTAC 2 cut(s) 162, 314
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 1 cut(s) 475
AluBI AGCT 3 cut(s) 157, 295, 385
AluI AGCT 3 cut(s) 157, 295, 385
Alw26I GTCTC 2 cut(s) 172, 199
AlwI GGATC 2 cut(s) 140, 153
Ama87I CYCGRG 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 490
AsuC2I CCSGG 2 cut(s) 210, 211
AsuHPI GGTGA 1 cut(s) 14
AvaI CYCGRG 1 cut(s) 209
BamHI GGATCC 1 cut(s) 145
BarI GAAGNNNNNNTAC 2 cut(s) 30, 62
BauI CACGAG 2 cut(s) 78, 169
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 191, 280, 409
BcnI CCSGG 2 cut(s) 210, 211
BcoDI GTCTC 2 cut(s) 172, 199
BfaI CTAG 1 cut(s) 296
BisI GCNGC 1 cut(s) 408
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 1 cut(s) 409
Bme1390I CCNGG 2 cut(s) 210, 211
BmeT110I CYCGRG 1 cut(s) 209
BmiI GGNNCC 1 cut(s) 147
BmrFI CCNGG 2 cut(s) 210, 211
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 2 cut(s) 318, 350
Bpu1102I GCTNAGC 1 cut(s) 386
BpuMI CCSGG 2 cut(s) 210, 211
BsaI GGTCTC 2 cut(s) 172, 199
BsaJI CCNNGG 1 cut(s) 209
BsaXI ACNNNNNCTCC 1 cut(s) 490
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 122, 246
Bse3DI GCAATG 1 cut(s) 109
BseDI CCNNGG 1 cut(s) 209
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 132, 204
BseNI ACTGG 2 cut(s) 122, 246
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 52
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 151
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 172, 199
BsmFI GGGAC 1 cut(s) 151
Bso31I GGTCTC 2 cut(s) 172, 199
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 3 cut(s) 145, 220, 337
Bsp1720I GCTNAGC 1 cut(s) 386
BspACI CCGC 2 cut(s) 50, 152
BspCNI CTCAG 2 cut(s) 133, 203
BspLI GGNNCC 1 cut(s) 147
BspPI GGATC 2 cut(s) 140, 153
BspTNI GGTCTC 2 cut(s) 172, 199
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 2 cut(s) 122, 246
BssECI CCNNGG 1 cut(s) 209
BssMI GATC 3 cut(s) 145, 220, 337
BssSI CACGAG 2 cut(s) 78, 169
Bst2BI CACGAG 2 cut(s) 78, 169
BstAPI GCANNNNNTGC 1 cut(s) 251
BstDEI CTNAG 3 cut(s) 141, 190, 386
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 3 cut(s) 148, 223, 340
BstMAI GTCTC 2 cut(s) 172, 199
BstMBI GATC 3 cut(s) 145, 220, 337
BstMWI GCNNNNNNNGC 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 208, 209
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 239
CfoI GCGC 1 cut(s) 490
Cfr9I CCCGGG 1 cut(s) 209
Csp6I GTAC 2 cut(s) 161, 313
CviJI RGCY 8 cut(s) 5, 131, 157, 175, 189, 295, 385, 471
CviKI_1 RGCY 8 cut(s) 5, 131, 157, 175, 189, 295, 385, 471
CviQI GTAC 2 cut(s) 161, 313
DdeI CTNAG 3 cut(s) 141, 190, 386
DpnI GATC 3 cut(s) 147, 222, 339
DpnII GATC 3 cut(s) 145, 220, 337
Eco31I GGTCTC 2 cut(s) 172, 199
Eco88I CYCGRG 1 cut(s) 209
EcoT22I ATGCAT 1 cut(s) 256
FaiI YATR 8 cut(s) 8, 47, 97, 219, 272, 365, 441, 483
FaqI GGGAC 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 408
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 1 cut(s) 296
FspI TGCGCA 1 cut(s) 489
GlaI GCGC 1 cut(s) 489
GluI GCNGC 1 cut(s) 408
GsaI CCCAGC 1 cut(s) 56
HapII CCGG 1 cut(s) 210
HhaI GCGC 1 cut(s) 490
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 1 cut(s) 505
HpaII CCGG 1 cut(s) 210
HphI GGTGA 1 cut(s) 14
Hpy188III TCNNGA 3 cut(s) 283, 341, 371
HpyAV CCTTC 3 cut(s) 238, 303, 434
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 2 cut(s) 254, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 251
HpyF3I CTNAG 3 cut(s) 141, 190, 386
HpySE526I ACGT 1 cut(s) 354
HspAI GCGC 1 cut(s) 488
Kzo9I GATC 3 cut(s) 145, 220, 337
LmnI GCTCC 1 cut(s) 154
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 241
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 2 cut(s) 181, 413
MalI GATC 3 cut(s) 147, 222, 339
MboI GATC 3 cut(s) 145, 220, 337
MboII GAAGA 2 cut(s) 50, 487
MflI RGATCY 1 cut(s) 145
MluCI AATT 1 cut(s) 366
MlyI GAGTC 1 cut(s) 499
MmeI TCCRAC 1 cut(s) 242
MnlI CCTC 5 cut(s) 27, 74, 136, 226, 461
Mph1103I ATGCAT 1 cut(s) 256
MseI TTAA 2 cut(s) 87, 330
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 52
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 210, 211
MwoI GCNNNNNNNGC 1 cut(s) 251
NciI CCSGG 2 cut(s) 210, 211
NdeII GATC 3 cut(s) 145, 220, 337
NlaIV GGNNCC 1 cut(s) 147
NmuCI GTSAC 1 cut(s) 413
NsbI TGCGCA 1 cut(s) 489
NsiI ATGCAT 1 cut(s) 256
PkrI GCNGC 1 cut(s) 409
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PsiI TTATAA 2 cut(s) 365, 483
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 147
PsuI RGATCY 1 cut(s) 145
RsaI GTAC 2 cut(s) 162, 314
RsaNI GTAC 2 cut(s) 161, 313
RseI CAYNNNNRTG 1 cut(s) 275
SaqAI TTAA 2 cut(s) 87, 330
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 3 cut(s) 145, 220, 337
SchI GAGTC 1 cut(s) 499
ScrFI CCNGG 2 cut(s) 210, 211
SfaNI GCATC 1 cut(s) 241
SmaI CCCGGG 1 cut(s) 211
SmiMI CAYNNNNRTG 1 cut(s) 275
Sse9I AATT 1 cut(s) 366
SsiI CCGC 2 cut(s) 50, 152
SspI AATATT 1 cut(s) 91
SspMI CTAG 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 208, 209
TaiI ACGT 1 cut(s) 357
TaqI TCGA 2 cut(s) 231, 336
TasI AATT 1 cut(s) 366
Tru1I TTAA 2 cut(s) 87, 330
Tru9I TTAA 2 cut(s) 87, 330
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 1 cut(s) 407
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 3 cut(s) 123, 416, 456
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 1 cut(s) 246
XmaI CCCGGG 1 cut(s) 209
XspI CTAG 1 cut(s) 296
Zsp2I ATGCAT 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.