MD05G1338500.v1.1

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003688
Physical Location & Seq
Reverse (-)
45990409 .. 45990852
444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1338500.v1.1.491

Sequence Viewer

Length: 303 bp
ATGAGACAGCAAGGCAGTGTACCATCTTCTGTTATATCCAGTCACTGTATGCATCTTGGTGTCCTCGCAACTGCATGGCACGCCATTTTAACAGGAACCATGTTCACTGTGTACTACAAGCCAAGGACAAGTCCTGCTGAGTTCATTGTTCCATTTGATCAATACATGGAGTCTGTTGAAAACAACTATTCTATAGGCATGCGGTTTAAGATGAGGTTTGAAGGTGAAGAAGCTCCACTCTTATTATCCTTTTTCTTGGATGCAAAGTATTCACGGGTCACATTTTTTTTCTCTTGGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0007568 GO:0008150 GO:0008285 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009838 GO:0009889 GO:0009890 GO:0009892 GO:0009900 GO:0009908 GO:0009909 GO:0009911 GO:0010033 GO:0010047 GO:0010150 GO:0010227 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010959 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032504 GO:0032879 GO:0033993 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035670 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043565 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048366 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048609 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051050 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090693 GO:0097159 GO:0097305 GO:0099402 GO:0140110 GO:1901363 GO:1901379 GO:1901381 GO:1901700 GO:1902679 GO:1903286 GO:1903288 GO:1903506 GO:1903507 GO:1904062 GO:1904064 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

101

Amino Acids

11.56

Weight (kDa)

6.82

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARF_AD PF06507 48 - 79 2.7e-09 Auxin response factor ancillary domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 202
AfaI GTAC 2 cut(s) 21, 113
AgsI TTSAA 2 cut(s) 179, 221
AluBI AGCT 1 cut(s) 233
AluI AGCT 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 45
AoxI GGCC 1 cut(s) 296
AspS9I GGNCC 1 cut(s) 297
AsuHPI GGTGA 1 cut(s) 236
BccI CCATC 1 cut(s) 31
BclI TGATCA 1 cut(s) 157
BfaI CTAG 1 cut(s) 301
BfmI CTRYAG 1 cut(s) 192
BmgT120I GGNCC 1 cut(s) 297
BmiI GGNNCC 1 cut(s) 97
BmsI GCATC 2 cut(s) 61, 250
BsaJI CCNNGG 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 1 cut(s) 265
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 39
BshFI GGCC 1 cut(s) 298
BsnI GGCC 1 cut(s) 298
Bsp143I GATC 1 cut(s) 157
BspACI CCGC 1 cut(s) 202
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 1 cut(s) 130
BspLI GGNNCC 1 cut(s) 97
BsrI ACTGG 1 cut(s) 39
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 122
Bst4CI ACNGT 2 cut(s) 47, 109
BstC8I GCNNGC 2 cut(s) 81, 200
BstDEI CTNAG 1 cut(s) 138
BstF5I GGATG 1 cut(s) 265
BstKTI GATC 1 cut(s) 160
BstMBI GATC 1 cut(s) 157
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 202
BstSFI CTRYAG 1 cut(s) 192
BsuRI GGCC 1 cut(s) 298
BtsCI GGATG 1 cut(s) 265
BtsI GCAGTG 1 cut(s) 22
BtsIMutI CAGTG 3 cut(s) 22, 43, 105
Cac8I GCNNGC 2 cut(s) 81, 200
CaiI CAGNNNCTG 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 297
Csp6I GTAC 2 cut(s) 20, 112
CviAII CATG 4 cut(s) 75, 100, 166, 199
CviJI RGCY 3 cut(s) 121, 233, 298
CviKI_1 RGCY 3 cut(s) 121, 233, 298
CviQI GTAC 2 cut(s) 20, 112
DdeI CTNAG 1 cut(s) 138
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 122
EcoT22I ATGCAT 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 122
FaeI CATG 4 cut(s) 78, 103, 169, 202
FaiI YATR 7 cut(s) 35, 50, 76, 101, 167, 194, 200
FatI CATG 4 cut(s) 74, 99, 165, 198
FbaI TGATCA 1 cut(s) 157
FokI GGATG 1 cut(s) 272
FspBI CTAG 1 cut(s) 301
HaeIII GGCC 1 cut(s) 298
Hin1II CATG 4 cut(s) 78, 103, 169, 202
HinfI GANTC 1 cut(s) 170
HphI GGTGA 1 cut(s) 236
Hpy166II GTNNAC 3 cut(s) 20, 105, 112
Hpy8I GTNNAC 3 cut(s) 20, 105, 112
HpyAV CCTTC 1 cut(s) 215
HpyCH4III ACNGT 2 cut(s) 47, 109
HpyCH4V TGCA 3 cut(s) 52, 74, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 138
Hsp92II CATG 4 cut(s) 78, 103, 169, 202
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 1 cut(s) 157
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 3 cut(s) 52, 78, 147
LweI GCATC 2 cut(s) 61, 250
MaeI CTAG 1 cut(s) 301
MaeIII GTNAC 2 cut(s) 41, 277
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 2 cut(s) 18, 239
MlyI GAGTC 1 cut(s) 179
MnlI CCTC 2 cut(s) 74, 207
Mph1103I ATGCAT 1 cut(s) 54
MseI TTAA 2 cut(s) 89, 207
MslI CAYNNNNRTG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 1 cut(s) 157
NlaIII CATG 4 cut(s) 78, 103, 169, 202
NlaIV GGNNCC 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 41, 277
NsiI ATGCAT 1 cut(s) 54
NspI RCATGY 1 cut(s) 202
PaeI GCATGC 1 cut(s) 202
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 297
PstNI CAGNNNCTG 1 cut(s) 45
RsaI GTAC 2 cut(s) 21, 113
RsaNI GTAC 2 cut(s) 20, 112
RseI CAYNNNNRTG 1 cut(s) 57
SaqAI TTAA 2 cut(s) 89, 207
Sau3AI GATC 1 cut(s) 157
Sau96I GGNCC 1 cut(s) 297
SchI GAGTC 1 cut(s) 179
SetI ASST 3 cut(s) 218, 226, 235
SfaNI GCATC 2 cut(s) 61, 250
SfcI CTRYAG 1 cut(s) 192
SmiMI CAYNNNNRTG 1 cut(s) 57
SphI GCATGC 1 cut(s) 202
SsiI CCGC 1 cut(s) 202
SspMI CTAG 1 cut(s) 301
StyI CCWWGG 1 cut(s) 122
TaaI ACNGT 2 cut(s) 47, 109
TatI WGTACW 1 cut(s) 111
Tru1I TTAA 2 cut(s) 89, 207
Tru9I TTAA 2 cut(s) 89, 207
TscAI CASTG 3 cut(s) 22, 50, 112
TseFI GTSAC 2 cut(s) 41, 277
Tsp45I GTSAC 2 cut(s) 41, 277
TspDTI ATGAA 1 cut(s) 133
TspRI CASTG 3 cut(s) 22, 50, 112
XceI RCATGY 1 cut(s) 202
XspI CTAG 1 cut(s) 301
Zsp2I ATGCAT 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.