Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0003688

Physical Location & Seq

Genomic Coordinates Reverse (-)

22123886 .. 22125861

Gene Structure 1976 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g21940.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1263 bp

ATGCAACATGGCATACTTGCCAGCGCTTTCCATGCCATTTCTACTGGTACCATGTTTACTGTGTACTACCACCCATGGACGAGTCCTGCTGCATTCATAATTCCTTTTGATCGATACATGAAATCAGCCGAAAATGACTATTCCATTGGAATGAGATTCAGAATGTATTCCGAAGGTGAAGAATGTGCAGAGAAGAAGAGGTTGGAAGGTACTATAATAGATATCGAAGATAACGATTGCTTGATGTGGCCCAGTTCAGAATGGAGGTGTCTGAAGGTGCGATTGGATACTACATCAGATACATGCTTGCATTCCGAAAGGCTTTCTCCTTGGAATATCATACCTCTCCAACCTAAGAAGAGGGCACATTTACCTGATCCGCCATCACCTGGTGTTTCTAGCTTGGGCAGGAAAGGTTCATTGGCGATTTCAGTTGAGTCCACACCTCCAAGACAAAAGAAGGTCTTTCAAGGTCAAGAAACTAGTGATCCACCTGCTCATGAACCAGTTACACCAAAACAGCTATTACTGCCACAGTTCATCCCACCCCCAAATCCTGACTGGGACTACACAGATTTGGGATTGGAAAACAATCTCAACGTTCCAATGAACGACCCATTTTACCAATGTCCTGGCTATCCAAAAGCTCCAAGCCTTACTAACCAATGGCCACCAATGTTTGGTTTTGGAGTCTTTGACAGTGGCACGTATAAAAGAAGCATGTCAGTTCCAAATATCAGCTCCTCGGTGTCCCAGAAATTGAGAGCCTTCGAACTGAGGAGTGAGACTCAATCGCCACTTGTCGAACAAAAGAGTACAATGCTTTTTGGAGTCAATATAGTTACCAGTCACCCAGAGCTCCCTTCACCACAAGTTGCCAATTCTATTGAACTTTGTAGTCCTTGTTCTATTCCTCCAATATCTCATTCAAGTGTTTCTGAAACAGTCCAGCTGTCAGAGACATCAAAGAGTATCTCTGGCGTTCTTTCTGAGAAACAATGCAAGAAATGTAGCTCCGTCAATAACAGGAGTTGCATAAAGGTGCTCAAATATGGAGCTCCTGTTGGAAGATCAATTGACCTCACGCGCTTTGATGGGTACAACGAACTCATTTGTGAGCTTGACCAGATGTTCGATTTCAAAGGAAGCCTGATCGATGGAAACAGTGGATGCCAGGTAACCTACATGGATGATGAAGGAGACAGGATGTTGATAGGAGATTACCCGTGGCAGTTAAGAACTAGAACTCATGCTGTGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

421

Amino Acids

46.95

Weight (kDa)

5.77

Isoelectric Point (pI)

54.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BRWD_AD	PF25313	14 - 117	6.5e-07	BRWD/PHIP ancillary domain-like domain
ARF_AD	PF06507	32 - 116	2.9e-25	Auxin response factor ancillary domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003688)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g24730 FvH4_2g24730 FvH4_2g24730 FvH4_5g06540
malus_domestica	MD00G1056300.v1.1 MD05G1338500.v1.1 MD11G1297900.v1.1
prunus_persica	Prupe.8G252300_v2.0.a1
pyrus_communis	pycom02g21520 pycom03g21940 pycom03g21950 pycom05g12140 pycom11g26170 pycom16g06400 pycom16g24090
rosa_chinensis	RchiOBHm_Chr6g0292551
rosa_laevigata	RLG00000012010
rosa_multiflora	Rmu_co8118566.1_g000001 Rmu_sc0000515.1_g000001 Rmu_sc0003486.1_g000005 Rmu_sc0004825.1_g000018
rosa_roxburghii	Rroxscaffold_4G00318020 Rroxscaffold_7G00174940
rosa_rugosa	Rorug06G0227900
rosa_samantha	Rh5AG357200 Rh5DG382900 Rh6AG341000 Rh6AG341100 Rh6BG089400 Rh6BG348500 Rh6CG355300 Rh6CG355400 Rh6DG341100 Rh6DG341200
rosa_wichuraiana	Rw6G029710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	502
Acc36I	ACCTGC	1 cut(s)	502
Acc65I	GGTACC	1 cut(s)	47
AccB1I	GGYRCC	1 cut(s)	47
AccB7I	CCANNNNNTGG	2 cut(s)	389, 680
AccII	CGCG	1 cut(s)	1087
AciI	CCGC	1 cut(s)	380
AclI	AACGTT	1 cut(s)	600
AclWI	GGATC	2 cut(s)	371, 482
AcoI	YGGCCR	1 cut(s)	668
AcuI	CTGAAG	1 cut(s)	293
AdeI	CACNNNGTG	1 cut(s)	392
AfaI	GTAC	5 cut(s)	49, 65, 211, 817, 1100
AfeI	AGCGCT	1 cut(s)	25
AfiI	CCNNNNNNNGG	2 cut(s)	389, 680
AgsI	TTSAA	4 cut(s)	470, 890, 930, 1141
AhlI	ACTAGT	1 cut(s)	482
AjnI	CCWGG	3 cut(s)	388, 631, 1173
AjuI	GAANNNNNNNTTGG	8 cut(s)	185, 217, 266, 298, 404, 436, 910, 942
AluBI	AGCT	9 cut(s)	402, 523, 647, 741, 859, 952, 1014, 1058, 1120
AluI	AGCT	9 cut(s)	402, 523, 647, 741, 859, 952, 1014, 1058, 1120
Alw21I	GWGCWC	3 cut(s)	861, 1047, 1060
Alw26I	GTCTC	3 cut(s)	779, 953, 1194
AlwI	GGATC	2 cut(s)	371, 482
Aor51HI	AGCGCT	1 cut(s)	25
AoxI	GGCC	2 cut(s)	248, 668
ApeKI	GCWGC	1 cut(s)	89
ArsI	GACNNNNNNTTYG	2 cut(s)	1115, 1147
Asp700I	GAANNNNTTC	1 cut(s)	166
Asp718I	GGTACC	1 cut(s)	47
AspLEI	GCGC	2 cut(s)	26, 1089
AspS9I	GGNCC	1 cut(s)	249
AsuHPI	GGTGA	4 cut(s)	188, 378, 842, 858
AsuII	TTCGAA	1 cut(s)	771
BaeGI	GKGCMC	1 cut(s)	367
BalI	TGGCCA	1 cut(s)	670
BanI	GGYRCC	1 cut(s)	47
BanII	GRGCYC	2 cut(s)	861, 1060
Bbv12I	GWGCWC	3 cut(s)	861, 1047, 1060
BbvI	GCAGC	1 cut(s)	76
BccI	CCATC	3 cut(s)	391, 1088, 1151
BciT130I	CCWGG	3 cut(s)	390, 633, 1175
BciVI	GTATCC	1 cut(s)	280
BcoDI	GTCTC	3 cut(s)	779, 953, 1194
BcuI	ACTAGT	1 cut(s)	482
BfaI	CTAG	3 cut(s)	399, 483, 1242
BfoI	RGCGCY	1 cut(s)	27
BfuAI	ACCTGC	1 cut(s)	502
BfuI	GTATCC	1 cut(s)	280
BisI	GCNGC	1 cut(s)	90
BlsI	GCNGC	1 cut(s)	91
Bme1390I	CCNGG	3 cut(s)	390, 633, 1175
BmgT120I	GGNCC	1 cut(s)	249
BmiI	GGNNCC	1 cut(s)	49
BmrFI	CCNGG	3 cut(s)	390, 633, 1175
BmrI	ACTGGG	2 cut(s)	246, 571
BmsI	GCATC	1 cut(s)	1160
BmuI	ACTGGG	2 cut(s)	246, 571
BplI	GAGNNNNNCTC	2 cut(s)	772, 804
Bpu14I	TTCGAA	1 cut(s)	771
Bsa29I	ATCGAT	2 cut(s)	112, 1155
BsaAI	YACGTR	1 cut(s)	708
BsaJI	CCNNGG	4 cut(s)	74, 329, 744, 1226
BsaXI	ACNNNNNCTCC	2 cut(s)	1047, 1077
Bsc4I	CCNNNNNNNGG	2 cut(s)	389, 680
Bse1I	ACTGG	5 cut(s)	49, 252, 506, 566, 846
BseBI	CCWGG	3 cut(s)	390, 633, 1175
BseCI	ATCGAT	2 cut(s)	112, 1155
BseDI	CCNNGG	4 cut(s)	74, 329, 744, 1226
BseGI	GGATG	4 cut(s)	540, 1175, 1195, 1212
BseLI	CCNNNNNNNGG	2 cut(s)	389, 680
BseMII	CTCAG	2 cut(s)	767, 981
BseNI	ACTGG	5 cut(s)	49, 252, 506, 566, 846
BseRI	GAGGAG	2 cut(s)	733, 793
BseSI	GKGCMC	1 cut(s)	367
BseXI	GCAGC	1 cut(s)	76
BsgI	GTGCAG	1 cut(s)	207
Bsh1236I	CGCG	1 cut(s)	1087
BshFI	GGCC	2 cut(s)	250, 670
BshNI	GGYRCC	1 cut(s)	47
BshVI	ATCGAT	2 cut(s)	112, 1155
BsiHKAI	GWGCWC	3 cut(s)	861, 1047, 1060
BslFI	GGGAC	2 cut(s)	578, 736
BslI	CCNNNNNNNGG	2 cut(s)	389, 680
BsmAI	GTCTC	3 cut(s)	779, 953, 1194
BsmFI	GGGAC	2 cut(s)	578, 736
BsmI	GAATGC	2 cut(s)	92, 310
BsnI	GGCC	2 cut(s)	250, 670
Bsp119I	TTCGAA	1 cut(s)	771
Bsp1286I	GDGCHC	4 cut(s)	367, 861, 1047, 1060
Bsp143I	GATC	5 cut(s)	109, 376, 487, 1070, 1152
Bsp19I	CCATGG	1 cut(s)	74
BspACI	CCGC	1 cut(s)	380
BspANI	GGCC	2 cut(s)	250, 670
BspCNI	CTCAG	2 cut(s)	768, 982
BspDI	ATCGAT	2 cut(s)	112, 1155
BspFNI	CGCG	1 cut(s)	1087
BspHI	TCATGA	1 cut(s)	499
BspLI	GGNNCC	1 cut(s)	49
BspMI	ACCTGC	1 cut(s)	502
BspPI	GGATC	2 cut(s)	371, 482
BspT104I	TTCGAA	1 cut(s)	771
BspT107I	GGYRCC	1 cut(s)	47
BsrI	ACTGG	5 cut(s)	49, 252, 506, 566, 846
BssECI	CCNNGG	4 cut(s)	74, 329, 744, 1226
BssMI	GATC	5 cut(s)	109, 376, 487, 1070, 1152
BssT1I	CCWWGG	2 cut(s)	74, 329
Bst2UI	CCWGG	3 cut(s)	390, 633, 1175
Bst4CI	ACNGT	5 cut(s)	61, 537, 701, 946, 1166
Bst6I	CTCTTC	2 cut(s)	191, 353
BstBAI	YACGTR	1 cut(s)	708
BstBI	TTCGAA	1 cut(s)	771
BstC8I	GCNNGC	2 cut(s)	22, 308
BstDEI	CTNAG	3 cut(s)	354, 776, 990
BstDSI	CCRYGG	2 cut(s)	74, 1226
BstEII	GGTNACC	1 cut(s)	1177
BstF5I	GGATG	4 cut(s)	540, 1175, 1195, 1212
BstFNI	CGCG	1 cut(s)	1087
BstH2I	RGCGCY	1 cut(s)	27
BstHHI	GCGC	2 cut(s)	26, 1089
BstKTI	GATC	5 cut(s)	112, 379, 490, 1073, 1155
BstMAI	GTCTC	3 cut(s)	779, 953, 1194
BstMBI	GATC	5 cut(s)	109, 376, 487, 1070, 1152
BstMWI	GCNNNNNNNGC	2 cut(s)	32, 529
BstNI	CCWGG	3 cut(s)	390, 633, 1175
BstNSI	RCATGY	2 cut(s)	306, 724
BstPI	GGTNACC	1 cut(s)	1177
BstSCI	CCNGG	3 cut(s)	388, 631, 1173
BstSLI	GKGCMC	1 cut(s)	367
BstUI	CGCG	1 cut(s)	1087
BstV1I	GCAGC	1 cut(s)	76
BstXI	CCANNNNNNTGG	1 cut(s)	632
Bsu15I	ATCGAT	2 cut(s)	112, 1155
BsuI	GTATCC	1 cut(s)	280
BsuRI	GGCC	2 cut(s)	250, 670
BsuTUI	ATCGAT	2 cut(s)	112, 1155
BtgI	CCRYGG	2 cut(s)	74, 1226
BtsCI	GGATG	4 cut(s)	540, 1175, 1195, 1212
BtsIMutI	CAGTG	2 cut(s)	706, 1171
BveI	ACCTGC	1 cut(s)	502
Cac8I	GCNNGC	2 cut(s)	22, 308
CciI	TCATGA	1 cut(s)	499
CfoI	GCGC	2 cut(s)	26, 1089
Cfr13I	GGNCC	1 cut(s)	249
ClaI	ATCGAT	2 cut(s)	112, 1155
CsiI	ACCWGGT	1 cut(s)	388
Csp6I	GTAC	5 cut(s)	48, 64, 210, 816, 1099
CviQI	GTAC	5 cut(s)	48, 64, 210, 816, 1099
DdeI	CTNAG	3 cut(s)	354, 776, 990
DpnI	GATC	5 cut(s)	111, 378, 489, 1072, 1154
DpnII	GATC	5 cut(s)	109, 376, 487, 1070, 1152
DraIII	CACNNNGTG	1 cut(s)	392
EaeI	YGGCCR	1 cut(s)	668
Eam1104I	CTCTTC	2 cut(s)	191, 353
EarI	CTCTTC	2 cut(s)	191, 353
EciI	GGCGGA	1 cut(s)	369
Ecl136II	GAGCTC	2 cut(s)	859, 1058
Eco130I	CCWWGG	2 cut(s)	74, 329
Eco24I	GRGCYC	2 cut(s)	861, 1060
Eco32I	GATATC	1 cut(s)	223
Eco47III	AGCGCT	1 cut(s)	25
Eco53kI	GAGCTC	2 cut(s)	859, 1058
Eco57I	CTGAAG	1 cut(s)	293
Eco91I	GGTNACC	1 cut(s)	1177
EcoICRI	GAGCTC	2 cut(s)	859, 1058
EcoO65I	GGTNACC	1 cut(s)	1177
EcoRII	CCWGG	3 cut(s)	388, 631, 1173
EcoRV	GATATC	1 cut(s)	223
EcoT14I	CCWWGG	2 cut(s)	74, 329
EcoT38I	GRGCYC	2 cut(s)	861, 1060
ErhI	CCWWGG	2 cut(s)	74, 329
FalI	AAGNNNNNCTT	2 cut(s)	449, 481
FaqI	GGGAC	2 cut(s)	578, 736
Fnu4HI	GCNGC	1 cut(s)	90
FokI	GGATG	4 cut(s)	527, 1182, 1202, 1219
FriOI	GRGCYC	2 cut(s)	861, 1060
Fsp4HI	GCNGC	1 cut(s)	90
FspBI	CTAG	3 cut(s)	399, 483, 1242
GlaI	GCGC	2 cut(s)	25, 1088
GluI	GCNGC	1 cut(s)	90
HaeII	RGCGCY	1 cut(s)	27
HaeIII	GGCC	2 cut(s)	250, 670
HhaI	GCGC	2 cut(s)	26, 1089
Hin6I	GCGC	2 cut(s)	24, 1087
HinP1I	GCGC	2 cut(s)	24, 1087
HinfI	GANTC	6 cut(s)	82, 156, 437, 690, 787, 831
HphI	GGTGA	4 cut(s)	188, 378, 842, 858
Hpy166II	GTNNAC	3 cut(s)	57, 64, 441
Hpy188I	TCNGA	9 cut(s)	161, 172, 259, 273, 298, 316, 940, 958, 991
Hpy188III	TCNNGA	3 cut(s)	476, 500, 557
Hpy8I	GTNNAC	3 cut(s)	57, 64, 441
HpyAV	CCTTC	7 cut(s)	167, 200, 268, 454, 778, 873, 1190
HpyCH4III	ACNGT	5 cut(s)	61, 537, 701, 946, 1166
HpyCH4IV	ACGT	2 cut(s)	600, 707
HpyCH4V	TGCA	6 cut(s)	4, 92, 188, 310, 1002, 1035
HpyF10VI	GCNNNNNNNGC	2 cut(s)	32, 529
HpyF3I	CTNAG	3 cut(s)	354, 776, 990
HpySE526I	ACGT	2 cut(s)	600, 707
HspAI	GCGC	2 cut(s)	24, 1087
KpnI	GGTACC	1 cut(s)	51
Kzo9I	GATC	5 cut(s)	109, 376, 487, 1070, 1152
LmnI	GCTCC	6 cut(s)	652, 746, 864, 1019, 1055, 1063
Lsp1109I	GCAGC	1 cut(s)	76
LweI	GCATC	1 cut(s)	1160
MabI	ACCWGGT	1 cut(s)	388
MaeI	CTAG	3 cut(s)	399, 483, 1242
MaeII	ACGT	2 cut(s)	600, 707
MaeIII	GTNAC	5 cut(s)	508, 841, 848, 1177, 1255
MalI	GATC	5 cut(s)	111, 378, 489, 1072, 1154
MboI	GATC	5 cut(s)	109, 376, 487, 1070, 1152
MboII	GAAGA	6 cut(s)	191, 205, 208, 239, 370, 1080
MfeI	CAATTG	1 cut(s)	1074
MhlI	GDGCHC	4 cut(s)	367, 861, 1047, 1060
MlsI	TGGCCA	1 cut(s)	670
MluCI	AATT	4 cut(s)	99, 758, 880, 1074
MluNI	TGGCCA	1 cut(s)	670
MlyI	GAGTC	5 cut(s)	91, 446, 699, 781, 840
MmeI	TCCRAC	3 cut(s)	183, 373, 1045
MnlI	CCTC	9 cut(s)	192, 258, 354, 354, 456, 754, 771, 924, 1091
Mox20I	TGGCCA	1 cut(s)	670
MroXI	GAANNNNTTC	1 cut(s)	166
MscI	TGGCCA	1 cut(s)	670
MseI	TTAA	2 cut(s)	1235, 1261
MslI	CAYNNNNRTG	3 cut(s)	149, 930, 1040
Msp20I	TGGCCA	1 cut(s)	670
MspA1I	CMGCKG	1 cut(s)	952
MspR9I	CCNGG	3 cut(s)	390, 633, 1175
MunI	CAATTG	1 cut(s)	1074
Mva1269I	GAATGC	2 cut(s)	92, 310
MvaI	CCWGG	3 cut(s)	390, 633, 1175
MvnI	CGCG	1 cut(s)	1087
MwoI	GCNNNNNNNGC	2 cut(s)	32, 529
NcoI	CCATGG	1 cut(s)	74
NdeII	GATC	5 cut(s)	109, 376, 487, 1070, 1152
NlaIV	GGNNCC	1 cut(s)	49
NmuCI	GTSAC	2 cut(s)	848, 1255
NspI	RCATGY	2 cut(s)	306, 724
NspV	TTCGAA	1 cut(s)	771
PagI	TCATGA	1 cut(s)	499
PaqCI	CACCTGC	1 cut(s)	502
PcsI	WCGNNNNNNNCGW	1 cut(s)	231
PctI	GAATGC	2 cut(s)	92, 310
PdmI	GAANNNNTTC	1 cut(s)	166
PfeI	GAWTC	1 cut(s)	156
PflMI	CCANNNNNTGG	2 cut(s)	389, 680
PkrI	GCNGC	1 cut(s)	91
PleI	GAGTC	5 cut(s)	90, 445, 698, 781, 839
PpsI	GAGTC	5 cut(s)	90, 445, 698, 781, 839
Ppu21I	YACGTR	1 cut(s)	708
Psp124BI	GAGCTC	2 cut(s)	861, 1060
Psp1406I	AACGTT	1 cut(s)	600
Psp6I	CCWGG	3 cut(s)	388, 631, 1173
PspEI	GGTNACC	1 cut(s)	1177
PspGI	CCWGG	3 cut(s)	388, 631, 1173
PspN4I	GGNNCC	1 cut(s)	49
PspPI	GGNCC	1 cut(s)	249
PsrI	GAACNNNNNNTAC	2 cut(s)	889, 921
PvuII	CAGCTG	1 cut(s)	952
RsaI	GTAC	5 cut(s)	49, 65, 211, 817, 1100
RsaNI	GTAC	5 cut(s)	48, 64, 210, 816, 1099
RseI	CAYNNNNRTG	3 cut(s)	149, 930, 1040
SacI	GAGCTC	2 cut(s)	861, 1060
SaqAI	TTAA	2 cut(s)	1235, 1261
SatI	GCNGC	1 cut(s)	90
Sau3AI	GATC	5 cut(s)	109, 376, 487, 1070, 1152
Sau96I	GGNCC	1 cut(s)	249
SchI	GAGTC	5 cut(s)	91, 446, 699, 781, 840
ScrFI	CCNGG	3 cut(s)	390, 633, 1175
SduI	GDGCHC	4 cut(s)	367, 861, 1047, 1060
SexAI	ACCWGGT	1 cut(s)	388
SfaNI	GCATC	1 cut(s)	1160
SfuI	TTCGAA	1 cut(s)	771
SmiMI	CAYNNNNRTG	3 cut(s)	149, 930, 1040
SpeI	ACTAGT	1 cut(s)	482
Sse9I	AATT	4 cut(s)	99, 758, 880, 1074
SsiI	CCGC	1 cut(s)	380
SspMI	CTAG	3 cut(s)	399, 483, 1242
SstI	GAGCTC	2 cut(s)	861, 1060
StyD4I	CCNGG	3 cut(s)	388, 631, 1173
StyI	CCWWGG	2 cut(s)	74, 329
TaaI	ACNGT	5 cut(s)	61, 537, 701, 946, 1166
TaiI	ACGT	2 cut(s)	603, 710
TaqI	TCGA	6 cut(s)	112, 225, 771, 804, 1134, 1155
TasI	AATT	4 cut(s)	99, 758, 880, 1074
TatI	WGTACW	2 cut(s)	63, 815
TfiI	GAWTC	1 cut(s)	156
Tru1I	TTAA	2 cut(s)	1235, 1261
Tru9I	TTAA	2 cut(s)	1235, 1261
TscAI	CASTG	2 cut(s)	706, 1171
TseFI	GTSAC	2 cut(s)	848, 1255
TseI	GCWGC	1 cut(s)	89
Tsp45I	GTSAC	2 cut(s)	848, 1255
TspDTI	ATGAA	7 cut(s)	85, 134, 408, 516, 529, 623, 1209
TspGWI	ACGGA	1 cut(s)	1006
TspRI	CASTG	2 cut(s)	706, 1171
Van91I	CCANNNNNTGG	2 cut(s)	389, 680
XceI	RCATGY	2 cut(s)	306, 724
XcmI	CCANNNNNNNNNTGG	1 cut(s)	558
XmnI	GAANNNNTTC	1 cut(s)	166
XspI	CTAG	3 cut(s)	399, 483, 1242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.