Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6C

Orthogroup ID

OG0003688

Physical Location & Seq

Genomic Coordinates Reverse (-)

54369276 .. 54371269

Gene Structure 1994 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6CG355300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGAAATCACCCAAAGATGAATATTCTGTTGGGATGAGATTCAGAATGCGGTTTGAAGCTGAAGAATGTGCAGAGAAGAGATGTGAAGGTACCATAATAGATATTAAAGATAAGGATCCTGTTAGGTGGCCCAGTTCTGAATGGGGGTGTCTGAAGGTGCAATGGGATACCACATCAGCTACGCTCTTGCATTCTGAAAGGCTTTCTCCTTGGAACATTGAGCCTGCAGAATCCACCAAGACGAAGAGGACTTCTTTCCCTGTCCCACCTCATAAGAGACCACGTATACCTGATCTATCATTACCTGTGTTTTCTGGGTTGGCCAGGAATGGCCCATTTCCATTAATAATGCCTCAAAGACGAGAAAAGGTCTTTCAAGGTCAAGAAATAAGCGAAACACATGCTAATGAACTAGGTGCACCAATGTATGATCCGCTACACCAAAGTCCTAGCAGTTCAATAGCATTTTCCAGTGAAAATATGCTGACTCCAGGCCACATTAACCAATGGCCATCACAGTTGACTACTTTTGGGGTCGTTGACAGTGTTCCATTTAGCAGAAGCATGTCGGTTCCAAATATCAACTCCTCTGGATCCCAGGAATTAAGCGCCTCTAAACTGAGGAGTGAGACTCAAGGGCCATTTGTTCCATCAAATACCCTCATGCTTTTTGGAGTCAATTTAGATAACAGTCACTCGGAGCTCCCTTCACCACAAGTTGCCAATTCTAGTGGACATTTAAGTCCTTGTTCTATTCCTCCAATTTCTCAGTCAAGTGTTTCTGAAACTGTCCAACTTTCAGAGACATCTAAGAGTATTTCTTGTGTTCTTTCTGAGACACAATGCAAGAAATGTTGTTCTGTCAGCAACAGGAGTTGCATAAAGGTGCTCAAGTATGGAGCAGCCCTTGGAAGATCAGTGGACCTCACACGCTTTGATGGGTATGGTGAGCTTATTTCTGAGCTTGACCAGATGTTCGATTTTGAAGGAAGGTTGATCGATGGAAGTAGTGGATGGCAGGTAACCTACATGGACGATGAAGGAGACATGATGCTGATAGGAGATTATCTGTGGCATGAATTCCTGTCAATGGTGCGAAAAATGTTCATCAGTCCAAAGGATCAAGAAATCGATAGTCTGAATCCAGGCTCTCCAAATGCAACATCCCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

AUX_IAA Auxin_resp B3

Protein Analysis

390

Amino Acids

43.3

Weight (kDa)

5.39

Isoelectric Point (pI)

67.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARF_AD	PF06507	3 - 76	7.2e-27	Auxin response factor ancillary domain
AUX_IAA	PF02309	331 - 370	7.2e-06	AUX/IAA family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003688)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g24730 FvH4_2g24730 FvH4_2g24730 FvH4_5g06540
malus_domestica	MD00G1056300.v1.1 MD05G1338500.v1.1 MD11G1297900.v1.1
prunus_persica	Prupe.8G252300_v2.0.a1
pyrus_communis	pycom02g21520 pycom03g21940 pycom03g21950 pycom05g12140 pycom11g26170 pycom16g06400 pycom16g24090
rosa_chinensis	RchiOBHm_Chr6g0292551
rosa_laevigata	RLG00000012010
rosa_multiflora	Rmu_co8118566.1_g000001 Rmu_sc0000515.1_g000001 Rmu_sc0003486.1_g000005 Rmu_sc0004825.1_g000018
rosa_roxburghii	Rroxscaffold_4G00318020 Rroxscaffold_7G00174940
rosa_rugosa	Rorug06G0227900
rosa_samantha	Rh5AG357200 Rh5DG382900 Rh6AG341000 Rh6AG341100 Rh6BG089400 Rh6BG348500 Rh6CG355300 Rh6CG355400 Rh6DG341100 Rh6DG341200
rosa_wichuraiana	Rw6G029710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	741
Acc36I	ACCTGC	1 cut(s)	1009
Acc65I	GGTACC	1 cut(s)	89
AccB1I	GGYRCC	1 cut(s)	89
AccI	GTMKAC	1 cut(s)	286
AciI	CCGC	2 cut(s)	49, 434
AclWI	GGATC	6 cut(s)	110, 123, 425, 588, 601, 1128
AcoI	YGGCCR	2 cut(s)	321, 509
AcsI	RAATTY	1 cut(s)	1079
AcuI	CTGAAG	2 cut(s)	81, 173
AfaI	GTAC	1 cut(s)	91
AfiI	CCNNNNNNNGG	1 cut(s)	1090
AgsI	TTSAA	4 cut(s)	56, 377, 459, 986
AjnI	CCWGG	4 cut(s)	323, 490, 597, 1144
AjuI	GAANNNNNNNTTGG	2 cut(s)	12, 44
AluBI	AGCT	5 cut(s)	59, 179, 703, 952, 964
AluI	AGCT	5 cut(s)	59, 179, 703, 952, 964
Alw21I	GWGCWC	3 cut(s)	421, 705, 891
Alw26I	GTCTC	5 cut(s)	271, 623, 797, 830, 1038
Alw44I	GTGCAC	1 cut(s)	417
AlwI	GGATC	6 cut(s)	110, 123, 425, 588, 601, 1128
AoxI	GGCC	6 cut(s)	128, 321, 331, 493, 509, 638
ApaLI	GTGCAC	1 cut(s)	417
ApeKI	GCWGC	1 cut(s)	902
ApoI	RAATTY	1 cut(s)	1079
ArsI	GACNNNNNNTTYG	2 cut(s)	959, 991
AseI	ATTAAT	1 cut(s)	344
Asp718I	GGTACC	1 cut(s)	89
AspLEI	GCGC	1 cut(s)	611
AspS9I	GGNCC	4 cut(s)	129, 332, 638, 922
AsuHPI	GGTGA	2 cut(s)	702, 959
AvaII	GGWCC	1 cut(s)	922
BaeGI	GKGCMC	1 cut(s)	421
BalI	TGGCCA	2 cut(s)	323, 511
BamHI	GGATCC	2 cut(s)	115, 593
BanI	GGYRCC	1 cut(s)	89
BanII	GRGCYC	1 cut(s)	705
Bbv12I	GWGCWC	3 cut(s)	421, 705, 891
BbvI	GCAGC	1 cut(s)	914
BccI	CCATC	5 cut(s)	520, 658, 932, 995, 1008
BcgI	CGANNNNNNTGC	2 cut(s)	383, 417
BciT130I	CCWGG	4 cut(s)	325, 492, 599, 1146
BciVI	GTATCC	1 cut(s)	160
BcoDI	GTCTC	5 cut(s)	271, 623, 797, 830, 1038
BfaI	CTAG	3 cut(s)	413, 450, 729
BfmI	CTRYAG	1 cut(s)	225
BfoI	RGCGCY	1 cut(s)	612
BfuAI	ACCTGC	1 cut(s)	1009
BfuI	GTATCC	1 cut(s)	160
BisI	GCNGC	1 cut(s)	903
BlsI	GCNGC	1 cut(s)	904
Bme1390I	CCNGG	4 cut(s)	325, 492, 599, 1146
Bme18I	GGWCC	1 cut(s)	922
BmgT120I	GGNCC	4 cut(s)	129, 332, 638, 922
BmiI	GGNNCC	4 cut(s)	91, 117, 573, 595
BmrFI	CCNGG	4 cut(s)	325, 492, 599, 1146
BmrI	ACTGGG	1 cut(s)	126
BmsI	GCATC	1 cut(s)	1041
BmuI	ACTGGG	1 cut(s)	126
BplI	GAGNNNNNCTC	2 cut(s)	616, 648
BpmI	CTGGAG	1 cut(s)	474
BpuEI	CTTGAG	2 cut(s)	618, 875
Bsa29I	ATCGAT	2 cut(s)	999, 1131
BsaAI	YACGTR	1 cut(s)	284
BsaBI	GATNNNNATC	1 cut(s)	114
BsaI	GGTCTC	1 cut(s)	271
BsaJI	CCNNGG	3 cut(s)	209, 597, 907
Bsc4I	CCNNNNNNNGG	1 cut(s)	1090
Bse1I	ACTGG	2 cut(s)	132, 471
Bse3DI	GCAATG	1 cut(s)	167
Bse8I	GATNNNNATC	1 cut(s)	114
BseBI	CCWGG	4 cut(s)	325, 492, 599, 1146
BseCI	ATCGAT	2 cut(s)	999, 1131
BseDI	CCNNGG	3 cut(s)	209, 597, 907
BseGI	GGATG	3 cut(s)	39, 1019, 1163
BseJI	GATNNNNATC	1 cut(s)	114
BseLI	CCNNNNNNNGG	1 cut(s)	1090
BseMI	GCAATG	1 cut(s)	167
BseMII	CTCAG	4 cut(s)	611, 782, 825, 951
BseNI	ACTGG	2 cut(s)	132, 471
BseRI	GAGGAG	2 cut(s)	577, 637
BseSI	GKGCMC	1 cut(s)	421
BseXI	GCAGC	1 cut(s)	914
BsgI	GTGCAG	1 cut(s)	90
BshFI	GGCC	6 cut(s)	130, 323, 333, 495, 511, 640
BshNI	GGYRCC	1 cut(s)	89
BshVI	ATCGAT	2 cut(s)	999, 1131
BsiHKAI	GWGCWC	3 cut(s)	421, 705, 891
BslFI	GGGAC	1 cut(s)	248
BslI	CCNNNNNNNGG	1 cut(s)	1090
BsmAI	GTCTC	5 cut(s)	271, 623, 797, 830, 1038
BsmFI	GGGAC	1 cut(s)	248
BsmI	GAATGC	2 cut(s)	51, 190
BsnI	GGCC	6 cut(s)	130, 323, 333, 495, 511, 640
Bso31I	GGTCTC	1 cut(s)	271
Bsp1286I	GDGCHC	3 cut(s)	421, 705, 891
Bsp143I	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
BspACI	CCGC	2 cut(s)	49, 434
BspANI	GGCC	6 cut(s)	130, 323, 333, 495, 511, 640
BspCNI	CTCAG	4 cut(s)	612, 781, 826, 952
BspDI	ATCGAT	2 cut(s)	999, 1131
BspLI	GGNNCC	4 cut(s)	91, 117, 573, 595
BspMAI	CTGCAG	1 cut(s)	229
BspMI	ACCTGC	1 cut(s)	1009
BspPI	GGATC	6 cut(s)	110, 123, 425, 588, 601, 1128
BspT107I	GGYRCC	1 cut(s)	89
BspTNI	GGTCTC	1 cut(s)	271
BsrDI	GCAATG	1 cut(s)	167
BsrI	ACTGG	2 cut(s)	132, 471
BssECI	CCNNGG	3 cut(s)	209, 597, 907
BssMI	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
BssNAI	GTATAC	1 cut(s)	287
BssT1I	CCWWGG	2 cut(s)	209, 907
Bst1107I	GTATAC	1 cut(s)	287
Bst2UI	CCWGG	4 cut(s)	325, 492, 599, 1146
Bst4CI	ACNGT	4 cut(s)	519, 545, 692, 790
Bst6I	CTCTTC	2 cut(s)	71, 239
BstBAI	YACGTR	1 cut(s)	284
BstC8I	GCNNGC	1 cut(s)	225
BstDEI	CTNAG	5 cut(s)	620, 768, 810, 834, 960
BstEII	GGTNACC	1 cut(s)	1021
BstF5I	GGATG	3 cut(s)	39, 1019, 1163
BstH2I	RGCGCY	1 cut(s)	612
BstHHI	GCGC	1 cut(s)	611
BstKTI	GATC	7 cut(s)	118, 295, 433, 596, 917, 999, 1123
BstMAI	GTCTC	5 cut(s)	271, 623, 797, 830, 1038
BstMBI	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
BstNI	CCWGG	4 cut(s)	325, 492, 599, 1146
BstNSI	RCATGY	2 cut(s)	404, 568
BstPI	GGTNACC	1 cut(s)	1021
BstSCI	CCNGG	4 cut(s)	323, 490, 597, 1144
BstSFI	CTRYAG	1 cut(s)	225
BstSLI	GKGCMC	1 cut(s)	421
BstV1I	GCAGC	1 cut(s)	914
BstX2I	RGATCY	2 cut(s)	115, 593
BstYI	RGATCY	2 cut(s)	115, 593
BstZ17I	GTATAC	1 cut(s)	287
Bsu15I	ATCGAT	2 cut(s)	999, 1131
BsuI	GTATCC	1 cut(s)	160
BsuRI	GGCC	6 cut(s)	130, 323, 333, 495, 511, 640
BsuTUI	ATCGAT	2 cut(s)	999, 1131
BtsCI	GGATG	3 cut(s)	39, 1019, 1163
BtsIMutI	CAGTG	3 cut(s)	478, 550, 924
BveI	ACCTGC	1 cut(s)	1009
Cac8I	GCNNGC	1 cut(s)	225
CfoI	GCGC	1 cut(s)	611
Cfr13I	GGNCC	4 cut(s)	129, 332, 638, 922
ClaI	ATCGAT	2 cut(s)	999, 1131
Csp6I	GTAC	1 cut(s)	90
CspCI	CAANNNNNGTGG	2 cut(s)	712, 747
CviAII	CATG	6 cut(s)	401, 565, 664, 1030, 1048, 1076
CviQI	GTAC	1 cut(s)	90
DdeI	CTNAG	5 cut(s)	620, 768, 810, 834, 960
DpnI	GATC	7 cut(s)	117, 294, 432, 595, 916, 998, 1122
DpnII	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
DrdI	GACNNNNNNGTC	1 cut(s)	741
DseDI	GACNNNNNNGTC	1 cut(s)	741
EaeI	YGGCCR	2 cut(s)	321, 509
Eam1104I	CTCTTC	2 cut(s)	71, 239
EarI	CTCTTC	2 cut(s)	71, 239
Ecl136II	GAGCTC	1 cut(s)	703
Eco130I	CCWWGG	2 cut(s)	209, 907
Eco24I	GRGCYC	1 cut(s)	705
Eco31I	GGTCTC	1 cut(s)	271
Eco47I	GGWCC	1 cut(s)	922
Eco53kI	GAGCTC	1 cut(s)	703
Eco57I	CTGAAG	2 cut(s)	81, 173
Eco91I	GGTNACC	1 cut(s)	1021
EcoICRI	GAGCTC	1 cut(s)	703
EcoO65I	GGTNACC	1 cut(s)	1021
EcoRI	GAATTC	1 cut(s)	1079
EcoRII	CCWGG	4 cut(s)	323, 490, 597, 1144
EcoT14I	CCWWGG	2 cut(s)	209, 907
EcoT38I	GRGCYC	1 cut(s)	705
ErhI	CCWWGG	2 cut(s)	209, 907
FaeI	CATG	6 cut(s)	404, 568, 667, 1033, 1051, 1079
FaqI	GGGAC	1 cut(s)	248
FatI	CATG	6 cut(s)	400, 564, 663, 1029, 1047, 1075
FblI	GTMKAC	1 cut(s)	286
Fnu4HI	GCNGC	1 cut(s)	903
FokI	GGATG	3 cut(s)	46, 1026, 1150
FriOI	GRGCYC	1 cut(s)	705
Fsp4HI	GCNGC	1 cut(s)	903
FspBI	CTAG	3 cut(s)	413, 450, 729
GlaI	GCGC	1 cut(s)	610
GluI	GCNGC	1 cut(s)	903
GsuI	CTGGAG	1 cut(s)	474
HaeII	RGCGCY	1 cut(s)	612
HaeIII	GGCC	6 cut(s)	130, 323, 333, 495, 511, 640
HhaI	GCGC	1 cut(s)	611
Hin1II	CATG	6 cut(s)	404, 568, 667, 1033, 1051, 1079
Hin6I	GCGC	1 cut(s)	609
HinP1I	GCGC	1 cut(s)	609
HincII	GTYRAC	2 cut(s)	522, 541
HindII	GTYRAC	2 cut(s)	522, 541
HinfI	GANTC	6 cut(s)	39, 230, 487, 631, 675, 1141
HphI	GGTGA	2 cut(s)	702, 959
Hpy166II	GTNNAC	6 cut(s)	287, 419, 522, 541, 734, 922
Hpy188III	TCNNGA	3 cut(s)	383, 591, 1124
Hpy8I	GTNNAC	6 cut(s)	287, 419, 522, 541, 734, 922
HpyAV	CCTTC	6 cut(s)	80, 148, 717, 980, 984, 1034
HpyCH4III	ACNGT	4 cut(s)	519, 545, 692, 790
HpyCH4IV	ACGT	1 cut(s)	283
HpyCH4V	TGCA	8 cut(s)	71, 160, 190, 227, 419, 846, 879, 1160
HpyF3I	CTNAG	5 cut(s)	620, 768, 810, 834, 960
HpySE526I	ACGT	1 cut(s)	283
Hsp92II	CATG	6 cut(s)	404, 568, 667, 1033, 1051, 1079
HspAI	GCGC	1 cut(s)	609
KpnI	GGTACC	1 cut(s)	93
Kzo9I	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
LmnI	GCTCC	3 cut(s)	700, 708, 899
Lsp1109I	GCAGC	1 cut(s)	914
LweI	GCATC	1 cut(s)	1041
MaeI	CTAG	3 cut(s)	413, 450, 729
MaeII	ACGT	1 cut(s)	283
MaeIII	GTNAC	2 cut(s)	692, 1021
MalI	GATC	7 cut(s)	117, 294, 432, 595, 916, 998, 1122
MboI	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
MboII	GAAGA	4 cut(s)	74, 88, 256, 924
MflI	RGATCY	2 cut(s)	115, 593
MhlI	GDGCHC	3 cut(s)	421, 705, 891
MlsI	TGGCCA	2 cut(s)	323, 511
MluCI	AATT	5 cut(s)	602, 679, 724, 762, 1079
MluNI	TGGCCA	2 cut(s)	323, 511
MlyI	GAGTC	3 cut(s)	481, 625, 684
MmeI	TCCRAC	1 cut(s)	817
MnlI	CCTC	9 cut(s)	240, 279, 363, 598, 615, 622, 671, 768, 935
Mox20I	TGGCCA	2 cut(s)	323, 511
MscI	TGGCCA	2 cut(s)	323, 511
MseI	TTAA	5 cut(s)	105, 344, 501, 605, 740
MslI	CAYNNNNRTG	2 cut(s)	405, 884
Msp20I	TGGCCA	2 cut(s)	323, 511
MspR9I	CCNGG	4 cut(s)	325, 492, 599, 1146
Mva1269I	GAATGC	2 cut(s)	51, 190
MvaI	CCWGG	4 cut(s)	325, 492, 599, 1146
NdeII	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
NlaIII	CATG	6 cut(s)	404, 568, 667, 1033, 1051, 1079
NlaIV	GGNNCC	4 cut(s)	91, 117, 573, 595
NmuCI	GTSAC	1 cut(s)	692
NspI	RCATGY	2 cut(s)	404, 568
PctI	GAATGC	2 cut(s)	51, 190
PfeI	GAWTC	3 cut(s)	39, 230, 1141
PkrI	GCNGC	1 cut(s)	904
PleI	GAGTC	3 cut(s)	481, 625, 683
PpsI	GAGTC	3 cut(s)	481, 625, 683
Ppu21I	YACGTR	1 cut(s)	284
PshBI	ATTAAT	1 cut(s)	344
Psp124BI	GAGCTC	1 cut(s)	705
Psp6I	CCWGG	4 cut(s)	323, 490, 597, 1144
PspEI	GGTNACC	1 cut(s)	1021
PspGI	CCWGG	4 cut(s)	323, 490, 597, 1144
PspN4I	GGNNCC	4 cut(s)	91, 117, 573, 595
PspPI	GGNCC	4 cut(s)	129, 332, 638, 922
PstI	CTGCAG	1 cut(s)	229
PsuI	RGATCY	2 cut(s)	115, 593
RsaI	GTAC	1 cut(s)	91
RsaNI	GTAC	1 cut(s)	90
RseI	CAYNNNNRTG	2 cut(s)	405, 884
SacI	GAGCTC	1 cut(s)	705
SaqAI	TTAA	5 cut(s)	105, 344, 501, 605, 740
SatI	GCNGC	1 cut(s)	903
Sau3AI	GATC	7 cut(s)	115, 292, 430, 593, 914, 996, 1120
Sau96I	GGNCC	4 cut(s)	129, 332, 638, 922
SchI	GAGTC	3 cut(s)	481, 625, 684
ScrFI	CCNGG	4 cut(s)	325, 492, 599, 1146
SduI	GDGCHC	3 cut(s)	421, 705, 891
SfaNI	GCATC	1 cut(s)	1041
SfcI	CTRYAG	1 cut(s)	225
SinI	GGWCC	1 cut(s)	922
SmiMI	CAYNNNNRTG	2 cut(s)	405, 884
SmlI	CTYRAG	2 cut(s)	633, 890
SmoI	CTYRAG	2 cut(s)	633, 890
Sse9I	AATT	5 cut(s)	602, 679, 724, 762, 1079
SsiI	CCGC	2 cut(s)	49, 434
SspI	AATATT	1 cut(s)	23
SspMI	CTAG	3 cut(s)	413, 450, 729
SstI	GAGCTC	1 cut(s)	705
StyD4I	CCNGG	4 cut(s)	323, 490, 597, 1144
StyI	CCWWGG	2 cut(s)	209, 907
TaaI	ACNGT	4 cut(s)	519, 545, 692, 790
TaiI	ACGT	1 cut(s)	286
TaqI	TCGA	3 cut(s)	978, 999, 1131
TasI	AATT	5 cut(s)	602, 679, 724, 762, 1079
TfiI	GAWTC	3 cut(s)	39, 230, 1141
Tru1I	TTAA	5 cut(s)	105, 344, 501, 605, 740
Tru9I	TTAA	5 cut(s)	105, 344, 501, 605, 740
TscAI	CASTG	3 cut(s)	478, 550, 924
TseFI	GTSAC	1 cut(s)	692
TseI	GCWGC	1 cut(s)	902
Tsp45I	GTSAC	1 cut(s)	692
TspDTI	ATGAA	6 cut(s)	17, 33, 423, 1053, 1092, 1096
TspRI	CASTG	3 cut(s)	478, 550, 924
VneI	GTGCAC	1 cut(s)	417
VpaK11BI	GGWCC	1 cut(s)	922
VspI	ATTAAT	1 cut(s)	344
XapI	RAATTY	1 cut(s)	1079
XceI	RCATGY	2 cut(s)	404, 568
XmiI	GTMKAC	1 cut(s)	286
XspI	CTAG	3 cut(s)	413, 450, 729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.