Rh5AG357200

Auxin response factors (ARFs) are transcriptional factors that bind specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003688
Physical Location & Seq
Reverse (-)
59408674 .. 59415936
7263 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG357200.1

Sequence Viewer

Length: 504 bp
ATGGTGAAGAACAGAGGATTGCAAGGAGTAGCATGTTATGGAGATTTTGGTCTCGGCAGAGCAGATATGGGTGGAGATGAAATCCCAGGTCAAGGAAGGTTAGCCGAGGCTACCAAAGGAAGGAGCTGGAGAGGTTATAAGAAAGGTCAGTCAAAGAGGCACTTGCTTACTAGTGGTTGGAGTACATTTGTGACTGCCAAGAAACTTGTTCTTGGGGATGCATGTATCTTTGTTAGGTATCTAACGATGGTTATACTGGTTTTGCAAAGGAGAAATGGAGAGCTCCCTGTTGGCATACGCCGTGTGACCAAAAGACAAGACAATGCAACAACTTCACTCATTTGTGGTAATAGCATGCAGCATGGCATCCTTGCGAGTGCTTTCCATGCCATTTCTACTGGAACCATGTTTACCGTGTACTATCGCCCTTGGACCAGTCCTGTGCCATTTCCAAGGTTTGAGAATTGTAAAAAAGAAAAGGTAGAGACCCTTTATGAGCGAGAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
AUX_IAA Auxin_resp B3

Protein Analysis

168

Amino Acids

18.81

Weight (kDa)

9.97

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 36 - 78 5e-09 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
AfaI GTAC 2 cut(s) 184, 419
AfiI CCNNNNNNNGG 2 cut(s) 92, 120
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 2 cut(s) 126, 283
AluI AGCT 2 cut(s) 126, 283
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 56, 479
ApeKI GCWGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 432
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 432
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 1 cut(s) 370
BccI CCATC 1 cut(s) 241
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 56, 479
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 1 cut(s) 171
BisI GCNGC 1 cut(s) 359
BlsI GCNGC 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 87
Bme18I GGWCC 1 cut(s) 432
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 87
BmsI GCATC 2 cut(s) 208, 375
BpmI CTGGAG 1 cut(s) 148
BsaI GGTCTC 2 cut(s) 56, 479
BsaJI CCNNGG 4 cut(s) 85, 105, 428, 452
Bsc4I CCNNNNNNNGG 2 cut(s) 92, 120
Bse1I ACTGG 3 cut(s) 261, 403, 435
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 4 cut(s) 85, 105, 428, 452
BseGI GGATG 2 cut(s) 223, 366
BseLI CCNNNNNNNGG 2 cut(s) 92, 120
BseNI ACTGG 3 cut(s) 261, 403, 435
BseXI GCAGC 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 285
BslI CCNNNNNNNGG 2 cut(s) 92, 120
BsmAI GTCTC 2 cut(s) 56, 479
Bso31I GGTCTC 2 cut(s) 56, 479
Bsp1286I GDGCHC 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 403
BspTNI GGTCTC 2 cut(s) 56, 479
BsrI ACTGG 3 cut(s) 261, 403, 435
BssECI CCNNGG 4 cut(s) 85, 105, 428, 452
BssT1I CCWWGG 2 cut(s) 428, 452
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 415
BstC8I GCNNGC 1 cut(s) 356
BstF5I GGATG 2 cut(s) 223, 366
BstMAI GTCTC 2 cut(s) 56, 479
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 87
BstNSI RCATGY 3 cut(s) 36, 225, 358
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 1 cut(s) 370
BtsCI GGATG 2 cut(s) 223, 366
Cac8I GCNNGC 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 432
Csp6I GTAC 2 cut(s) 183, 418
CviAII CATG 6 cut(s) 33, 222, 355, 362, 386, 406
CviJI RGCY 4 cut(s) 104, 110, 126, 283
CviKI_1 RGCY 4 cut(s) 104, 110, 126, 283
CviQI GTAC 2 cut(s) 183, 418
Ecl136II GAGCTC 1 cut(s) 283
Eco130I CCWWGG 2 cut(s) 428, 452
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 2 cut(s) 56, 479
Eco47I GGWCC 1 cut(s) 432
Eco53kI GAGCTC 1 cut(s) 283
EcoICRI GAGCTC 1 cut(s) 283
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 428, 452
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 1 cut(s) 285
ErhI CCWWGG 2 cut(s) 428, 452
FaeI CATG 6 cut(s) 36, 225, 358, 365, 389, 409
FalI AAGNNNNNCTT 2 cut(s) 146, 178
FatI CATG 6 cut(s) 32, 221, 354, 361, 385, 405
Fnu4HI GCNGC 1 cut(s) 359
FokI GGATG 2 cut(s) 230, 353
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 1 cut(s) 359
FspBI CTAG 1 cut(s) 171
GluI GCNGC 1 cut(s) 359
GsuI CTGGAG 1 cut(s) 148
Hin1II CATG 6 cut(s) 36, 225, 358, 365, 389, 409
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 2 cut(s) 411, 418
Hpy8I GTNNAC 2 cut(s) 411, 418
HpyAV CCTTC 2 cut(s) 90, 114
HpyCH4III ACNGT 1 cut(s) 415
HpyCH4V TGCA 5 cut(s) 22, 221, 265, 326, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
Hsp92II CATG 6 cut(s) 36, 225, 358, 365, 389, 409
LmnI GCTCC 2 cut(s) 123, 288
LpnPI CCDG 8 cut(s) 72, 99, 112, 242, 300, 384, 448, 453
Lsp1109I GCAGC 1 cut(s) 370
LweI GCATC 2 cut(s) 208, 375
MaeI CTAG 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 190, 304
MboII GAAGA 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 1 cut(s) 463
MmeI TCCRAC 1 cut(s) 158
MnlI CCTC 4 cut(s) 8, 100, 125, 150
Mph1103I ATGCAT 1 cut(s) 223
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 386
NlaIII CATG 6 cut(s) 36, 225, 358, 365, 389, 409
NlaIV GGNNCC 1 cut(s) 403
NmeAIII GCCGAG 2 cut(s) 33, 130
NmuCI GTSAC 2 cut(s) 190, 304
NsiI ATGCAT 1 cut(s) 223
NspI RCATGY 3 cut(s) 36, 225, 358
PaeI GCATGC 1 cut(s) 358
PkrI GCNGC 1 cut(s) 360
PsiI TTATAA 1 cut(s) 138
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 85
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 1 cut(s) 432
PsrI GAACNNNNNNTAC 2 cut(s) 394, 426
RsaI GTAC 2 cut(s) 184, 419
RsaNI GTAC 2 cut(s) 183, 418
SacI GAGCTC 1 cut(s) 285
SatI GCNGC 1 cut(s) 359
Sau96I GGNCC 1 cut(s) 432
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 285
SetI ASST 9 cut(s) 91, 101, 128, 136, 148, 239, 285, 458, 483
SfaNI GCATC 2 cut(s) 208, 375
SinI GGWCC 1 cut(s) 432
SpeI ACTAGT 1 cut(s) 170
SphI GCATGC 1 cut(s) 358
Sse9I AATT 1 cut(s) 463
SspMI CTAG 1 cut(s) 171
SstI GAGCTC 1 cut(s) 285
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 428, 452
TaaI ACNGT 1 cut(s) 415
TasI AATT 1 cut(s) 463
TatI WGTACW 2 cut(s) 182, 417
TseFI GTSAC 2 cut(s) 190, 304
TseI GCWGC 1 cut(s) 358
Tsp45I GTSAC 2 cut(s) 190, 304
TspDTI ATGAA 1 cut(s) 93
VpaK11BI GGWCC 1 cut(s) 432
XceI RCATGY 3 cut(s) 36, 225, 358
XspI CTAG 1 cut(s) 171
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.