MD06G1005900.v1.1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0001817
Physical Location & Seq
Reverse (-)
619871 .. 629712
9842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1005900.v1.1.491

Sequence Viewer

Length: 2880 bp
ATGGACCCCTCCATTATGAAGAAGCTGCTCGAAGACGATGAGGACGAAACCATGCATTCGGGGGCGGATGTGGAGGCCTTCCAGGCTGCCCTCAATAGGGACATTGAAGGAGATGCATCTGCTTCCCAGCCATCTGATTCCGACTCCGCGATTTTGTCTCAAGGAAGCAATAATACTTCTAGTCAGCCATTACCACAGTTCCAAACAACTGGTCAGGATGAAAGTACAGCATGCGAAATGCAACATGACCAAAAAATTGCACAACCGCGAGAACTCCATTCATATGAAATGGAGCTAAAGCAACAAGGATCTGTTGCTGAAAATATGCAGCTAAAAAGTGATGCCTCACAGGAACTTAGTCACTTTTCTTTGTCCCAGAAGCAACCCCAAGGTGATCTTCAACAAGGGCAAGCAGAACAAAAATCCCTCCAGGTACCTGAAACAACTGGCACGCCGATTTCTGGAAAAATTCCCGTCTCCAATCAGGAGCAGGACATTACACCTACTCCGCAAAGCGAATCTCAGTACTTAAAGCTTCAGAGGATGAGCAGTCAACAGGCAATGATCACTGAGCAGCCAAGCAACCCAATGAATCGTAGTAAACAAGTACCGTTTGGCCTGTTACTACCTGTCCTATTGCCCCAGCTTGATAAAGACAGGGCCATGCAACTTAATACTCTATTTGGCAAACTCAAGAATAATGAAATCTCAAAAGACGCTTTTGTTCGGCACATTCGAAGTGTTGTTGGGGACCAGATGCTCAAATTGGCAGTAATGAAAGTACAATCGCAGCCAGGTCCTAAACACCAGTTACTGCCTCAGGCTTCAGTGCAGCAACAGTCTCCTAAGATGCCATCCGCAATTGCAGGTTCCACACAGTTCCCTGATCCCCGTTCATTTGCACTTAATCAGAGAGGCACCAACTCTCCGACAGACCCATCACACATTCCATCTTCAACAGTTCAACTGCAGTCTGATTCAAGCCATTCAGTCATTGAAAACAGTGCTAAAAAACTGCGGGAGGCAGAACGTCAGTCAGACTCTCATGGAATGCAAGTCAGCCAAATGTCTTCTTCTAGTGTGGTAGCTGGGAATCAAGAAAGGGAGCGCTCATCTGGTCCTATGCAAATACTTAATAAGCAGCAACAACCGTTGCAGTATCCACAATCATCCTATTCCATGTATGGAAGTACTGGTGGTAATTATCACCCATATACGGGGACAAGTATCAATACATTGCCTCTCAAACAGCAACCTCATGATTCACAACTTAGGCAAATACCACAACATCAAAGCATGGGTTCAGCTCAGTCAGGTGGGGAAGCAAAGGGAGGGAACGTCATGGGTGTGTCCAATTTGGAGAGGCAAAATTTGATGAAGGACCCTAGTAGACTGCAGGGTGGATCTCTTTCGCACTTTACAAATAATTCAAATTTACAACAGAATATAGCTCCTTGGCAATCCTCAAATAAAGAGCCACATTCTGGTCCTTTGTCATCAATGCCTTTTGTAAAACAGGAACTGACTGATCAGACTGCTGAGCAGCAGCACAAACCCCCATTGTCTAATTCACAGGAATTGACTTCTGCTGCGAAACTTGAGCAGGGGAATGGGTCACCTGGAATTTCAATGGACGAGCCGTTAGATAAGCAATCTTCAAGAATGGGGTTTCCAGCATCTGTAAGCATTGGGGCTTCATCTTCTACAAGCACAGTGCCCCCGAATTCTGTTTCTTCTTCCATGACAACGCAAGCTGATCCCAACGTCTTGTTAGGCCATCGAATACCCTCTGGAACTTCTCCTGCTGGGATTAGTAATAGAGCACCTCCAAAAAAGCCTTCTGTTGGCCAAAAGAAGCCTCTTGAAGCACTTGGTTCTTCACCACCTCCGTCTAGTAAGAAGCAAAAGGTATCCGGGAACTTTTTGGATCAAAGCATTGAACAACTGAATGATGTGACTGCTGTTAGTGGAGTTAATCTTAGGGAAGAGGAAGAGCAGCTGTTTTCTGGGCCCAAGGAGGATAGTCGAGCTTCAGAAGCATCTCGAAAGTTTGTGCAAGAAGAAGAAGAGAGGCTGATTTTGCAGAAGGATCCCTTGCAGAAAAAGTTGGCAGAAATTATGATCAAATGTGGATTGAAAAGTGTAAGCTATGATGTGGAGCGGTGCTTGTCACTGTGTGTGGAGGAAAGAATGAGGGGACTAATAAATAATCTGATCAGACTGTCAAAGCAGAGAGTTGATGCTGAGAAGCCAAGACACCGCACTATTATTACCTCTGATGTTCGGCAGCAAGTTATGAATCTCAACCAGAATGCCAGGGAAGAATTTGAAAAAAAGCAGGCCGAGGCAGAAAAACTTCGAAAGCTTAATGAACCTGAGGTTAGCAATGGAGTTGATGGTGATAAGGACAAAGATGAAGGTCGTTCGAAATCATTTAAGCCAAACAAAGAGGAAGATGACAAAATGAGGACAACAGCAGCAAATGTTGCTGCCCGAGCTGCTGTTGGAGGAGATGACATGTTGTCAAAATGGCAACTTATGGCTGAGCAAGCCCGGCAAAAACGTGAAGGTGGGATTGATGTAGCATCTGGGTCCCAGCCAGGTAAAGATGTAAACCGCAAGCCTACATTATCAGCAGGAAGAACTATGAAGGACAATCAAGAAGCAGAGAAAAGGAGCGGTGGAACTCCTGTTGCTTCAGCTGCCGGGACTATTAGAAAATGTGGAAAGAACCAAGTTAATGTGCCTCAAACGAAGGTGGCTCGTAGTATATCTGTTAAGGATGTGATTGCTGTCCTAGAGAGAGAACCTCAGATGTCGAGATCTACTATGATATATCGCTTGTTTGAGAGAGTTCAATCTAGCACCACTGGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

960

Amino Acids

104.89

Weight (kDa)

7.02

Isoelectric Point (pI)

60.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 198 - 262 5.9e-20 RCD1-SRO-TAF4 (RST) plant domain
TAF4 PF05236 649 - 948 4.6e-83 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 857
Acc65I GGTACC 1 cut(s) 433
AccB1I GGYRCC 2 cut(s) 433, 917
AccB7I CCANNNNNTGG 1 cut(s) 1484
AccBSI CCGCTC 2 cut(s) 2161, 2679
AccI GTMKAC 1 cut(s) 1390
AccII CGCG 2 cut(s) 149, 268
AclWI GGATC 7 cut(s) 316, 881, 1411, 1751, 1935, 2084, 2097
AcoI YGGCCR 1 cut(s) 1846
AcsI RAATTY 6 cut(s) 468, 1369, 1432, 1623, 1723, 2324
AcuI CTGAAG 4 cut(s) 521, 810, 2016, 2682
AdeI CACNNNGTG 1 cut(s) 2177
AfaI GTAC 6 cut(s) 226, 435, 527, 609, 783, 1192
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 7 cut(s) 96, 97, 461, 1216, 1217, 1484, 1844
AflIII ACRYGT 1 cut(s) 2517
AhdI GACNNNNNGTC 1 cut(s) 2521
AjnI CCWGG 6 cut(s) 81, 429, 793, 1618, 2315, 2599
AjuI GAANNNNNNNTTGG 4 cut(s) 381, 413, 1822, 1854
Alw21I GWGCWC 1 cut(s) 1825
Alw26I GTCTC 3 cut(s) 162, 481, 846
AlwI GGATC 7 cut(s) 316, 881, 1411, 1751, 1935, 2084, 2097
AlwNI CAGNNNCTG 3 cut(s) 814, 1522, 1679
Ama87I CYCGRG 1 cut(s) 2493
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 7 cut(s) 75, 616, 660, 1774, 1846, 2009, 2340
ApaI GGGCCC 1 cut(s) 2013
ApoI RAATTY 6 cut(s) 468, 1369, 1432, 1623, 1723, 2324
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
Asp700I GAANNNNTTC 1 cut(s) 2355
Asp718I GGTACC 1 cut(s) 433
AspLEI GCGC 1 cut(s) 1110
AsuC2I CCSGG 3 cut(s) 1915, 2554, 2707
AsuHPI GGTGA 5 cut(s) 404, 1199, 1608, 1872, 2411
AsuII TTCGAA 3 cut(s) 736, 2359, 2426
AvaI CYCGRG 1 cut(s) 2493
AvaII GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1487, 2592
AxyI CCTNAGG 2 cut(s) 819, 2376
BaeGI GKGCMC 2 cut(s) 1719, 2013
BalI TGGCCA 1 cut(s) 1848
BamHI GGATCC 1 cut(s) 2089
BanI GGYRCC 2 cut(s) 433, 917
BanII GRGCYC 1 cut(s) 2013
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BbsI GAAGAC 2 cut(s) 39, 1062
Bbv12I GWGCWC 1 cut(s) 1825
BccI CCATC 6 cut(s) 139, 862, 946, 958, 1785, 2390
BceAI ACGGC 1 cut(s) 1624
BciT130I CCWGG 6 cut(s) 83, 431, 795, 1620, 2317, 2601
BciVI GTATCC 2 cut(s) 1170, 1921
BclI TGATCA 4 cut(s) 564, 1528, 2121, 2214
BcnI CCSGG 3 cut(s) 1915, 2554, 2707
BcoDI GTCTC 3 cut(s) 162, 481, 846
BfaI CTAG 6 cut(s) 180, 1077, 1386, 1893, 2798, 2862
BfmI CTRYAG 2 cut(s) 968, 1394
BfoI RGCGCY 1 cut(s) 1111
BfuAI ACCTGC 1 cut(s) 857
BfuI GTATCC 2 cut(s) 1170, 1921
BglI GCCNNNNNGGC 1 cut(s) 83
BglII AGATCT 1 cut(s) 2822
BlpI GCTNAGC 2 cut(s) 1539, 2544
BmcAI AGTACT 2 cut(s) 527, 1192
Bme1390I CCNGG 9 cut(s) 83, 431, 795, 1620, 1915, 2317, 2554, 2601, 2707
Bme18I GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1487, 2592
BmeRI GACNNNNNGTC 1 cut(s) 2521
BmeT110I CYCGRG 1 cut(s) 2493
BmrFI CCNGG 9 cut(s) 83, 431, 795, 1620, 1915, 2317, 2554, 2601, 2707
BmsI GCATC 9 cut(s) 103, 125, 331, 747, 840, 1685, 2048, 2230, 2594
BpiI GAAGAC 2 cut(s) 39, 1062
BplI GAGNNNNNCTC 2 cut(s) 2794, 2826
BpmI CTGGAG 1 cut(s) 413
Bpu1102I GCTNAGC 2 cut(s) 1539, 2544
Bpu14I TTCGAA 3 cut(s) 736, 2359, 2426
BpuEI CTTGAG 3 cut(s) 144, 677, 1619
BpuMI CCSGG 3 cut(s) 1915, 2554, 2707
BsaJI CCNNGG 5 cut(s) 388, 1454, 2013, 2316, 2343
BsaXI ACNNNNNCTCC 6 cut(s) 490, 520, 910, 940, 1323, 1353
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 97, 461, 1216, 1217, 1484, 1844
Bse1I ACTGG 5 cut(s) 214, 451, 808, 1198, 2875
Bse21I CCTNAGG 2 cut(s) 819, 2376
Bse3DI GCAATG 3 cut(s) 567, 1235, 2392
BseBI CCWGG 6 cut(s) 83, 431, 795, 1620, 2317, 2601
BseDI CCNNGG 5 cut(s) 388, 1454, 2013, 2316, 2343
BseGI GGATG 6 cut(s) 73, 223, 549, 854, 1169, 2788
BseLI CCNNNNNNNGG 7 cut(s) 96, 97, 461, 1216, 1217, 1484, 1844
BseMI GCAATG 3 cut(s) 567, 1235, 2392
BseMII CTCAG 9 cut(s) 536, 561, 833, 1322, 1530, 2235, 2367, 2535, 2825
BseNI ACTGG 5 cut(s) 214, 451, 808, 1198, 2875
BseRI GAGGAG 1 cut(s) 2523
BseSI GKGCMC 2 cut(s) 1719, 2013
BseYI CCCAGC 5 cut(s) 126, 642, 1088, 1805, 2595
BsgI GTGCAG 1 cut(s) 851
Bsh1236I CGCG 2 cut(s) 149, 268
BshFI GGCC 7 cut(s) 77, 618, 662, 1776, 1848, 2011, 2342
BshNI GGYRCC 2 cut(s) 433, 917
BsiHKAI GWGCWC 1 cut(s) 1825
BsiHKCI CYCGRG 1 cut(s) 2493
BsiSI CCGG 3 cut(s) 1914, 2554, 2706
BslFI GGGAC 7 cut(s) 113, 358, 764, 1234, 2211, 2578, 2722
BslI CCNNNNNNNGG 7 cut(s) 96, 97, 461, 1216, 1217, 1484, 1844
BsmAI GTCTC 3 cut(s) 162, 481, 846
BsmBI CGTCTC 1 cut(s) 481
BsmFI GGGAC 7 cut(s) 113, 358, 764, 1234, 2211, 2578, 2722
BsmI GAATGC 3 cut(s) 55, 1056, 2317
BsnI GGCC 7 cut(s) 77, 618, 662, 1776, 1848, 2011, 2342
BsoBI CYCGRG 1 cut(s) 2493
Bsp119I TTCGAA 3 cut(s) 736, 2359, 2426
Bsp120I GGGCCC 1 cut(s) 2009
Bsp1286I GDGCHC 3 cut(s) 1719, 1825, 2013
Bsp1720I GCTNAGC 2 cut(s) 1539, 2544
BspANI GGCC 7 cut(s) 77, 618, 662, 1776, 1848, 2011, 2342
BspCNI CTCAG 9 cut(s) 535, 562, 832, 1321, 1531, 2236, 2368, 2536, 2824
BspFNI CGCG 2 cut(s) 149, 268
BspHI TCATGA 1 cut(s) 1258
BspMAI CTGCAG 2 cut(s) 972, 1398
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 7 cut(s) 316, 881, 1411, 1751, 1935, 2084, 2097
BspQI GCTCTTC 1 cut(s) 1986
BspT104I TTCGAA 3 cut(s) 736, 2359, 2426
BspT107I GGYRCC 2 cut(s) 433, 917
BsrBI CCGCTC 2 cut(s) 2161, 2679
BsrDI GCAATG 3 cut(s) 567, 1235, 2392
BsrI ACTGG 5 cut(s) 214, 451, 808, 1198, 2875
BssECI CCNNGG 5 cut(s) 388, 1454, 2013, 2316, 2343
BssT1I CCWWGG 3 cut(s) 388, 1454, 2013
Bst2UI CCWGG 6 cut(s) 83, 431, 795, 1620, 2317, 2601
Bst6I CTCTTC 3 cut(s) 1980, 1986, 2061
BstAPI GCANNNNNTGC 1 cut(s) 2486
BstBI TTCGAA 3 cut(s) 736, 2359, 2426
BstC8I GCNNGC 7 cut(s) 232, 411, 452, 1752, 2340, 2550, 2621
BstEII GGTNACC 1 cut(s) 1614
BstF5I GGATG 6 cut(s) 73, 223, 549, 854, 1169, 2788
BstFNI CGCG 2 cut(s) 149, 268
BstH2I RGCGCY 1 cut(s) 1111
BstHHI GCGC 1 cut(s) 1110
BstMAI GTCTC 3 cut(s) 162, 481, 846
BstMWI GCNNNNNNNGC 9 cut(s) 83, 2036, 2080, 2486, 2495, 2498, 2549, 2554, 2702
BstNI CCWGG 6 cut(s) 83, 431, 795, 1620, 2317, 2601
BstNSI RCATGY 2 cut(s) 234, 2521
BstPI GGTNACC 1 cut(s) 1614
BstSCI CCNGG 9 cut(s) 81, 429, 793, 1618, 1913, 2315, 2552, 2599, 2705
BstSFI CTRYAG 2 cut(s) 968, 1394
BstSLI GKGCMC 2 cut(s) 1719, 2013
BstUI CGCG 2 cut(s) 149, 268
BstV2I GAAGAC 2 cut(s) 39, 1062
BstX2I RGATCY 4 cut(s) 308, 1403, 2089, 2822
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 4 cut(s) 308, 1403, 2089, 2822
Bsu36I CCTNAGG 2 cut(s) 819, 2376
BsuI GTATCC 2 cut(s) 1170, 1921
BsuRI GGCC 7 cut(s) 77, 618, 662, 1776, 1848, 2011, 2342
BtsCI GGATG 6 cut(s) 73, 223, 549, 854, 1169, 2788
BtsIMutI CAGTG 6 cut(s) 567, 834, 1009, 1719, 2171, 2868
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 7 cut(s) 232, 411, 452, 1752, 2340, 2550, 2621
CaiI CAGNNNCTG 3 cut(s) 814, 1522, 1679
CciI TCATGA 1 cut(s) 1258
CfoI GCGC 1 cut(s) 1110
CseI GACGC 1 cut(s) 725
Csp6I GTAC 6 cut(s) 225, 434, 526, 608, 782, 1191
CviQI GTAC 6 cut(s) 225, 434, 526, 608, 782, 1191
DraIII CACNNNGTG 1 cut(s) 2177
DriI GACNNNNNGTC 1 cut(s) 2521
EaeI YGGCCR 1 cut(s) 1846
Eam1104I CTCTTC 3 cut(s) 1980, 1986, 2061
Eam1105I GACNNNNNGTC 1 cut(s) 2521
EarI CTCTTC 3 cut(s) 1980, 1986, 2061
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 3 cut(s) 388, 1454, 2013
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 2013
Eco47I GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1487, 2592
Eco47III AGCGCT 1 cut(s) 1109
Eco57I CTGAAG 4 cut(s) 521, 810, 2016, 2682
Eco81I CCTNAGG 2 cut(s) 819, 2376
Eco88I CYCGRG 1 cut(s) 2493
Eco91I GGTNACC 1 cut(s) 1614
EcoO109I RGGNCCY 3 cut(s) 797, 1381, 2592
EcoO65I GGTNACC 1 cut(s) 1614
EcoRI GAATTC 1 cut(s) 1723
EcoRII CCWGG 6 cut(s) 81, 429, 793, 1618, 2315, 2599
EcoT14I CCWWGG 3 cut(s) 388, 1454, 2013
EcoT22I ATGCAT 2 cut(s) 57, 118
EcoT38I GRGCYC 1 cut(s) 2013
ErhI CCWWGG 3 cut(s) 388, 1454, 2013
Esp3I CGTCTC 1 cut(s) 481
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 2078, 2110
FaqI GGGAC 7 cut(s) 113, 358, 764, 1234, 2211, 2578, 2722
FauI CCCGC 1 cut(s) 1011
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 4 cut(s) 564, 1528, 2121, 2214
FblI GTMKAC 1 cut(s) 1390
FokI GGATG 6 cut(s) 80, 230, 556, 841, 1156, 2795
FriOI GRGCYC 1 cut(s) 2013
FspBI CTAG 6 cut(s) 180, 1077, 1386, 1893, 2798, 2862
GlaI GCGC 1 cut(s) 1109
GsaI CCCAGC 5 cut(s) 130, 646, 1092, 1809, 2599
GsuI CTGGAG 1 cut(s) 413
HaeII RGCGCY 1 cut(s) 1111
HaeIII GGCC 7 cut(s) 77, 618, 662, 1776, 1848, 2011, 2342
HapII CCGG 3 cut(s) 1914, 2554, 2706
HgaI GACGC 1 cut(s) 725
HhaI GCGC 1 cut(s) 1110
Hin6I GCGC 1 cut(s) 1108
HinP1I GCGC 1 cut(s) 1108
HincII GTYRAC 1 cut(s) 554
HindII GTYRAC 1 cut(s) 554
HindIII AAGCTT 2 cut(s) 533, 2363
HinfI GANTC 9 cut(s) 137, 143, 518, 592, 977, 1040, 1093, 1262, 2299
HpaII CCGG 3 cut(s) 1914, 2554, 2706
HphI GGTGA 5 cut(s) 404, 1199, 1608, 1872, 2411
Hpy166II GTNNAC 4 cut(s) 554, 602, 1391, 2614
Hpy8I GTNNAC 4 cut(s) 554, 602, 1391, 2614
HpyAV CCTTC 9 cut(s) 88, 101, 1372, 1848, 2080, 2411, 2561, 2644, 2749
HpyCH4IV ACGT 4 cut(s) 1030, 1338, 1764, 2563
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 2036, 2080, 2486, 2495, 2498, 2549, 2554, 2702
HpySE526I ACGT 4 cut(s) 1030, 1338, 1764, 2563
HspAI GCGC 1 cut(s) 1108
KflI GGGWCCC 1 cut(s) 2592
KpnI GGTACC 1 cut(s) 437
Ksp22I TGATCA 4 cut(s) 564, 1528, 2121, 2214
LguI GCTCTTC 1 cut(s) 1986
LmnI GCTCC 6 cut(s) 292, 487, 1105, 1456, 2158, 2676
LweI GCATC 9 cut(s) 103, 125, 331, 747, 840, 1685, 2048, 2230, 2594
MaeI CTAG 6 cut(s) 180, 1077, 1386, 1893, 2798, 2862
MaeII ACGT 4 cut(s) 1030, 1338, 1764, 2563
MaeIII GTNAC 6 cut(s) 359, 621, 810, 1614, 1954, 2169
MbiI CCGCTC 2 cut(s) 2161, 2679
MfeI CAATTG 1 cut(s) 861
MflI RGATCY 4 cut(s) 308, 1403, 2089, 2822
MhlI GDGCHC 3 cut(s) 1719, 1825, 2013
MlsI TGGCCA 1 cut(s) 1848
MluNI TGGCCA 1 cut(s) 1848
MlyI GAGTC 2 cut(s) 137, 1034
MmeI TCCRAC 3 cut(s) 165, 953, 2485
Mox20I TGGCCA 1 cut(s) 1848
Mph1103I ATGCAT 2 cut(s) 57, 118
MroXI GAANNNNTTC 1 cut(s) 2355
MscI TGGCCA 1 cut(s) 1848
MseI TTAA 9 cut(s) 530, 672, 906, 1134, 1974, 2367, 2436, 2739, 2778
MslI CAYNNNNRTG 2 cut(s) 282, 1346
Msp20I TGGCCA 1 cut(s) 1848
MspA1I CMGCKG 2 cut(s) 1999, 2702
MspI CCGG 3 cut(s) 1914, 2554, 2706
MspR9I CCNGG 9 cut(s) 83, 431, 795, 1620, 1915, 2317, 2554, 2601, 2707
MunI CAATTG 1 cut(s) 861
Mva1269I GAATGC 3 cut(s) 55, 1056, 2317
MvaI CCWGG 6 cut(s) 83, 431, 795, 1620, 2317, 2601
MvnI CGCG 2 cut(s) 149, 268
MwoI GCNNNNNNNGC 9 cut(s) 83, 2036, 2080, 2486, 2495, 2498, 2549, 2554, 2702
NciI CCSGG 3 cut(s) 1915, 2554, 2707
NdeI CATATG 1 cut(s) 283
NmeAIII GCCGAG 1 cut(s) 2368
NmuCI GTSAC 4 cut(s) 359, 1614, 1954, 2169
NsiI ATGCAT 2 cut(s) 57, 118
NspI RCATGY 2 cut(s) 234, 2521
NspV TTCGAA 3 cut(s) 736, 2359, 2426
PaeI GCATGC 1 cut(s) 234
PagI TCATGA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 2517
PciSI GCTCTTC 1 cut(s) 1986
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 733
PctI GAATGC 3 cut(s) 55, 1056, 2317
PdmI GAANNNNTTC 1 cut(s) 2355
PfeI GAWTC 7 cut(s) 137, 518, 592, 977, 1093, 1262, 2299
PflMI CCANNNNNTGG 1 cut(s) 1484
PfoI TCCNGGA 1 cut(s) 1913
PleI GAGTC 2 cut(s) 137, 1034
PpsI GAGTC 2 cut(s) 137, 1034
PpuMI RGGWCCY 3 cut(s) 797, 1381, 2592
PscI ACATGT 1 cut(s) 2517
Psp5II RGGWCCY 3 cut(s) 797, 1381, 2592
Psp6I CCWGG 6 cut(s) 81, 429, 793, 1618, 2315, 2599
PspEI GGTNACC 1 cut(s) 1614
PspFI CCCAGC 5 cut(s) 126, 642, 1088, 1805, 2595
PspGI CCWGG 6 cut(s) 81, 429, 793, 1618, 2315, 2599
PspOMI GGGCCC 1 cut(s) 2009
PspPPI RGGWCCY 3 cut(s) 797, 1381, 2592
PstI CTGCAG 2 cut(s) 972, 1398
PstNI CAGNNNCTG 3 cut(s) 814, 1522, 1679
PsuI RGATCY 4 cut(s) 308, 1403, 2089, 2822
PvuII CAGCTG 2 cut(s) 1999, 2702
RsaI GTAC 6 cut(s) 226, 435, 527, 609, 783, 1192
RsaNI GTAC 6 cut(s) 225, 434, 526, 608, 782, 1191
RseI CAYNNNNRTG 2 cut(s) 282, 1346
SapI GCTCTTC 1 cut(s) 1986
SaqAI TTAA 9 cut(s) 530, 672, 906, 1134, 1974, 2367, 2436, 2739, 2778
ScaI AGTACT 2 cut(s) 527, 1192
SchI GAGTC 2 cut(s) 137, 1034
ScrFI CCNGG 9 cut(s) 83, 431, 795, 1620, 1915, 2317, 2554, 2601, 2707
SduI GDGCHC 3 cut(s) 1719, 1825, 2013
SfaNI GCATC 9 cut(s) 103, 125, 331, 747, 840, 1685, 2048, 2230, 2594
SfcI CTRYAG 2 cut(s) 968, 1394
SfuI TTCGAA 3 cut(s) 736, 2359, 2426
SinI GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1487, 2592
SmiMI CAYNNNNRTG 2 cut(s) 282, 1346
SmlI CTYRAG 3 cut(s) 159, 692, 1598
SmoI CTYRAG 3 cut(s) 159, 692, 1598
SphI GCATGC 1 cut(s) 234
SseBI AGGCCT 1 cut(s) 77
SspMI CTAG 6 cut(s) 180, 1077, 1386, 1893, 2798, 2862
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 9 cut(s) 81, 429, 793, 1618, 1913, 2315, 2552, 2599, 2705
StyI CCWWGG 3 cut(s) 388, 1454, 2013
TaiI ACGT 4 cut(s) 1033, 1341, 1767, 2566
TaqI TCGA 8 cut(s) 30, 736, 1780, 2026, 2044, 2359, 2426, 2819
TatI WGTACW 4 cut(s) 224, 525, 781, 1190
TfiI GAWTC 7 cut(s) 137, 518, 592, 977, 1093, 1262, 2299
Tru1I TTAA 9 cut(s) 530, 672, 906, 1134, 1974, 2367, 2436, 2739, 2778
Tru9I TTAA 9 cut(s) 530, 672, 906, 1134, 1974, 2367, 2436, 2739, 2778
TscAI CASTG 6 cut(s) 574, 834, 1009, 1719, 2178, 2875
TseFI GTSAC 4 cut(s) 359, 1614, 1954, 2169
Tsp45I GTSAC 4 cut(s) 359, 1614, 1954, 2169
TspGWI ACGGA 1 cut(s) 1878
TspRI CASTG 6 cut(s) 574, 834, 1009, 1719, 2178, 2875
Van91I CCANNNNNTGG 1 cut(s) 1484
VpaK11BI GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1487, 2592
XapI RAATTY 6 cut(s) 468, 1369, 1432, 1623, 1723, 2324
XceI RCATGY 2 cut(s) 234, 2521
XmiI GTMKAC 1 cut(s) 1390
XmnI GAANNNNTTC 1 cut(s) 2355
XspI CTAG 6 cut(s) 180, 1077, 1386, 1893, 2798, 2862
ZrmI AGTACT 2 cut(s) 527, 1192
Zsp2I ATGCAT 2 cut(s) 57, 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.