Prupe.5G007400_v2.0.a1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0001817
Physical Location & Seq
Forward (+)
784422 .. 793516
9095 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G007400.1

Sequence Viewer

Length: 3006 bp
ATGGACCCATCCATTATGAAGAAGCTCCTTGAAGACGATGAGGATGAAACCATGCATTCGGGGGCCGATGTGGAGGCCTTCCAGGCCGCTCTGAACAGGGACATAGAAGGAGATGTGTCTGTTTCGCAGCCGTCTGATTCAGACTCCGCAGTTTTGTCTCAAGGAAGCAATAACACTTCTAGTCAGTCATTACCGCAGTTCCATACTGCTACTCAGGATGAAAACACAGCTTGTCAAACGCAACATGACAAAAAAATTGCGCAACAGCGAGAAATGCATTCATATGAAATGGAGCTAAAGCAATATGGGTCCGGTGCTGAGAATATACAACAAAAAAAGGATGCCTCACATGAATTTAATCAATTTCCTTTGCCCCAGAAGCAACCCCAAGGTGATCTTCAACAAGGGCAGGCAGAGCAGAAACCCCTCCATAAACCTGAAACAGCTGGAATACCGATTTCTGGGAAAATTCCAATCTCAAAACATGAGCAGGACGTAACACCTACTCCAGAAAGCGAATCTCAGTACTTAAAGCTTCAGAAGATGAGTAGTCAACAGGCAATGATCCCGGAGCAGCCAAGCAACCCGATGAATCGCAGTAAACAAGTACCATTTGGCCTGTTACTCCCTGTCTTATTGCCCCAGCTTGATAAAGACAGAGCCATGCAACTTACTACTCTATTTGGTAAACTGAAGAACAATGAAATATCAAAGGATGCTTTTGTCCGGCACATTCGAAGTGTTGTTGGGGACCAGATGCTCAAATTGGCAGTAATGAAAGTACAATCACAGCCTGTTCCTAAACACCAGTTACCACCTCAGTCTTCAGTGCAGCAACAACCTCCTAGGATGCCATCTATTAGTGTAGGTTCTACACCGTTCACTGATCCTCGCTCATTTGCACTTCATCAGAGAGGCGCCAACCCTCCCACAGACCCATCACACATTCCCTCTTCAGCAGTTCAAGTGCAGTCTGATTCAAGCCATTCAGTCATCGAAAACAGTGCTAAAAAACTGCGGGAGGCAGAACGTCCGTCAGACTCTCATGGAATGCAAGTCAGTCAAATGCCTTCTTCTAGTGCGGTAGCTGGCAATCAAGAAAGGGAGCGCTCCTCAGGCCCTCCACAAATCCTTAATAAGCAGCAGCAGCAACAACAGTTGCACTATCCACAATCATCCTTTGCCATGTATGGAAGTACTGGTGGTAATTATCACCCGTATTCAGGGACAAGTATCAATACCTCTACATTGCCTCTCAAGCAGCAACCTCATGATTCACAACTGAGGCAAATTCCACAACATCAAGGCATGGGTTCAACTCAGTCAGGTGGGGAACCACAGGGTGTGAACATCACTAATGTGTCAAAATTGGAGAGGCAAAATTCTCTGAATGACCCTAGTAGACTGCAGGGTGGATCTGTTTCTCACTTTACAAATAATTCAAATTTACAACAGAATTCAGTTCCTCGGCAGTCCTCAAATAAGGAGCAGAATCCCGGTCCTGTGTCATCAATGTCTTATGTAAAACAGGAACCAATTGATCAGACTGCTGAGCAGCAGCAGAAGCCCCCATTATCTAATCAACAAGGACTGCCTTCTGCTTCTGCTGCTCAACTTGAGCAGGGGAGTGCTTTACCTGGAATTTCAACAGATGAGTCTATAGAGAAGCAATCTTCAAGAATGGGATTTGCAACATCTGGCATGGTGACTTCATCTTCTACAGGCACAGTGCCCCCAAATTCTGTTTCTCCTTCCATAATGACGCAAGTTGATACTAATGTCTCGTTAGGCCATCGAATACCCTCTGGAACTGCTGGGATTAGTAATAGAGCACCTCCAAAAAAGCCTTCTATTGGCCAAAAGAAGCCTCTTGAAGTACCTGGTTCCTCACCACCGCCATCAAGTAAGAAGCAAAAATTATCCGGGAACTTTTTGGATCAAAGCATTGAACAACTGAATGATGTCACTGCTGTTAGTGGAGTTAATCTTAGGGAAGAGGAAGAGCAGCTGTTTTCTGGACCCAAGGAGGATAGTCGAGCTTCAGAAGCATCCCGAAAGTTTGTGCAAGAAGAAGAAGAAAGGCTGATTTTGCAGAAGGCTCCCTTGCAGAAAAAATTAGCCGAAATTATGGTCAAATGTGGTTTGAAAAGTATAAGCAATGACGTGGAGCGGTGTTTGTCACTGTGTGTGGAGGAAAGAATGAGGGGACTAATAAATAATTTGATCAGACTGTCAAAGCAGCGGGTTGATGCTGAGAAACCAAGACACCACACTATTACTACCTCTGATGTTCGGCAACAAGTTATGAATCTCAACCAGAACGCTAGGGAAGAATTTGAAAAAAAGCAGGCTGAGGCAGAAAAGCTTCGAAGGCTTAATGAACCTGAGGTTAACAATGGAGTTGATGGTGATAAAGACAAAGATGATGGTCGCTCCAAATCATTTAAGCCAAACAAAGAGGAAGATGACAAAATGAGGACGACAGCAGCAAATGTTGCTGCCCGAGCTGCTGTTGGAGGGGATGACATGTTGTCAAAATGGCAACTTATGGCTGAGCAAGCCCGGCAAAAGCGTGAAGGTGGGGTTGATGTGGCATCTGGGTCCCAACCAGGTAAAGATGTAAACCGCAAGCCTACATCAACAGCTGGAAGAATTATGAAGGACAATCAGGAAGCAGAGAAAAGGGGAGGTGGAACTCCAGTTGCTGCAGCTGGGACTTTTAGAAAATGTGGAAGGAACCAAGTTATCACACCTCAAACAAGGGTGGCTCGGAGTATATCTGTCAAGGATGTGATTGCTGTCCTGGAGAGGGAACCTCAGATGTCGAGGTCTACTATGATATATCGCTTGTTTGAAAGAATCCAATCAGACACCACTGGCAGGTTCACCATTCCAAGTGCAAAGCTGGTCCATGTTGGTTCTACAGTTTCTGTCATGTACCATTTTATGATATTCATTTTTGTAATAAAAACATTGCATCTTACCCTGACTAGCAATATTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

1002

Amino Acids

109.92

Weight (kDa)

8.68

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 2871
AccB1I GGYRCC 1 cut(s) 917
AccBSI CCGCTC 2 cut(s) 89, 2170
AccI GTMKAC 2 cut(s) 1402, 2831
AciI CCGC 9 cut(s) 87, 147, 194, 1018, 1082, 1895, 2170, 2242, 2626
AclWI GGATC 4 cut(s) 559, 881, 1423, 1944
AcoI YGGCCR 1 cut(s) 1855
AcsI RAATTY 9 cut(s) 353, 468, 1290, 1381, 1444, 1456, 1641, 1738, 2333
AcuI CTGAAG 5 cut(s) 521, 713, 810, 939, 2025
AcyI GRCGYC 1 cut(s) 918
AdeI CACNNNGTG 2 cut(s) 1343, 2186
AfaI GTAC 6 cut(s) 527, 609, 783, 1198, 1878, 2939
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 5 cut(s) 461, 1223, 1853, 2809, 2880
AflIII ACRYGT 1 cut(s) 2526
AhdI GACNNNNNGTC 1 cut(s) 2530
AjiI CACGTC 1 cut(s) 2164
AjnI CCWGG 5 cut(s) 81, 1636, 1879, 2608, 2802
AjuI GAANNNNNNNTTGG 6 cut(s) 381, 413, 1729, 1761, 1831, 1863
AleI CACNNNNGTG 1 cut(s) 1358
Alw21I GWGCWC 1 cut(s) 1834
Alw26I GTCTC 2 cut(s) 162, 1786
AlwI GGATC 4 cut(s) 559, 881, 1423, 1944
AlwNI CAGNNNCTG 2 cut(s) 2705, 2930
Ama87I CYCGRG 1 cut(s) 2502
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 7 cut(s) 63, 75, 84, 616, 1117, 1789, 1855
ApoI RAATTY 9 cut(s) 353, 468, 1290, 1381, 1444, 1456, 1641, 1738, 2333
ArsI GACNNNNNNTTYG 2 cut(s) 2115, 2147
Asp700I GAANNNNTTC 1 cut(s) 2364
AspA2I CCTAGG 1 cut(s) 845
AspLEI GCGC 3 cut(s) 262, 920, 1110
AspS9I GGNCC 9 cut(s) 4, 63, 309, 751, 1118, 1499, 2018, 2601, 2908
AsuC2I CCSGG 4 cut(s) 569, 1497, 1924, 2563
AsuHPI GGTGA 6 cut(s) 404, 1205, 1717, 1881, 2420, 2878
AsuII TTCGAA 2 cut(s) 736, 2368
AvaI CYCGRG 1 cut(s) 2502
AvaII GGWCC 7 cut(s) 4, 309, 751, 1499, 2018, 2601, 2908
AvrII CCTAGG 1 cut(s) 845
AxyI CCTNAGG 2 cut(s) 1114, 2385
BaeGI GKGCMC 1 cut(s) 1734
BaeI ACNNNNGTAYC 2 cut(s) 1764, 1797
BalI TGGCCA 1 cut(s) 1857
BanI GGYRCC 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 39, 816
Bbv12I GWGCWC 1 cut(s) 1834
BbvCI CCTCAGC 1 cut(s) 2352
BccI CCATC 7 cut(s) 16, 862, 946, 1800, 1906, 2399, 2420
BceAI ACGGC 1 cut(s) 115
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciT130I CCWGG 5 cut(s) 83, 1638, 1881, 2610, 2804
BclI TGATCA 2 cut(s) 1540, 2223
BcnI CCSGG 4 cut(s) 569, 1497, 1924, 2563
BcoDI GTCTC 2 cut(s) 162, 1786
BfaI CTAG 6 cut(s) 180, 846, 1077, 1398, 2325, 2991
BfmI CTRYAG 5 cut(s) 1406, 1659, 1719, 2706, 2922
BfoI RGCGCY 2 cut(s) 921, 1111
BfuAI ACCTGC 1 cut(s) 2871
BglI GCCNNNNNGGC 1 cut(s) 83
BlnI CCTAGG 1 cut(s) 845
BlpI GCTNAGC 2 cut(s) 1551, 2553
BmcAI AGTACT 2 cut(s) 527, 1198
Bme1390I CCNGG 9 cut(s) 83, 569, 1497, 1638, 1881, 1924, 2563, 2610, 2804
Bme18I GGWCC 7 cut(s) 4, 309, 751, 1499, 2018, 2601, 2908
BmeRI GACNNNNNGTC 1 cut(s) 2530
BmeT110I CYCGRG 1 cut(s) 2502
BmgBI CACGTC 1 cut(s) 2164
BmgT120I GGNCC 9 cut(s) 4, 63, 309, 751, 1118, 1499, 2018, 2601, 2908
BmrFI CCNGG 9 cut(s) 83, 569, 1497, 1638, 1881, 1924, 2563, 2610, 2804
BmsI GCATC 8 cut(s) 331, 706, 747, 840, 2057, 2239, 2603, 2986
BpiI GAAGAC 2 cut(s) 39, 816
BplI GAGNNNNNCTC 4 cut(s) 1097, 1129, 2800, 2832
BpmI CTGGAG 3 cut(s) 492, 2682, 2825
Bpu10I CCTNAGC 1 cut(s) 2352
Bpu1102I GCTNAGC 2 cut(s) 1551, 2553
Bpu14I TTCGAA 2 cut(s) 736, 2368
BpuEI CTTGAG 3 cut(s) 144, 1241, 1637
BpuMI CCSGG 4 cut(s) 569, 1497, 1924, 2563
BsaHI GRCGYC 1 cut(s) 918
BsaJI CCNNGG 4 cut(s) 388, 845, 1466, 2022
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bsc4I CCNNNNNNNGG 5 cut(s) 461, 1223, 1853, 2809, 2880
Bse1I ACTGG 4 cut(s) 808, 1204, 2699, 2881
Bse21I CCTNAGG 2 cut(s) 1114, 2385
Bse3DI GCAATG 4 cut(s) 567, 1247, 2164, 2972
BseBI CCWGG 5 cut(s) 83, 1638, 1881, 2610, 2804
BseDI CCNNGG 4 cut(s) 388, 845, 1466, 2022
BseLI CCNNNNNNNGG 5 cut(s) 461, 1223, 1853, 2809, 2880
BseMI GCAATG 4 cut(s) 567, 1247, 2164, 2972
BseNI ACTGG 4 cut(s) 808, 1204, 2699, 2881
BseRI GAGGAG 1 cut(s) 1102
BseSI GKGCMC 1 cut(s) 1734
BseYI CCCAGC 3 cut(s) 642, 1814, 2711
BsgI GTGCAG 2 cut(s) 851, 989
BshFI GGCC 7 cut(s) 65, 77, 86, 618, 1119, 1791, 1857
BshNI GGYRCC 1 cut(s) 917
BsiHKAI GWGCWC 1 cut(s) 1834
BsiHKCI CYCGRG 1 cut(s) 2502
BsiSI CCGG 6 cut(s) 312, 569, 727, 1497, 1923, 2563
BslFI GGGAC 6 cut(s) 113, 764, 1240, 2220, 2587, 2728
BslI CCNNNNNNNGG 5 cut(s) 461, 1223, 1853, 2809, 2880
BsmAI GTCTC 2 cut(s) 162, 1786
BsmFI GGGAC 6 cut(s) 113, 764, 1240, 2220, 2587, 2728
BsmI GAATGC 3 cut(s) 55, 277, 1056
BsnI GGCC 7 cut(s) 65, 77, 86, 618, 1119, 1791, 1857
BsoBI CYCGRG 1 cut(s) 2502
Bsp119I TTCGAA 2 cut(s) 736, 2368
Bsp1286I GDGCHC 2 cut(s) 1734, 1834
Bsp143I GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
Bsp1720I GCTNAGC 2 cut(s) 1551, 2553
BspACI CCGC 9 cut(s) 87, 147, 194, 1018, 1082, 1895, 2170, 2242, 2626
BspANI GGCC 7 cut(s) 65, 77, 86, 618, 1119, 1791, 1857
BspHI TCATGA 1 cut(s) 1270
BspMAI CTGCAG 2 cut(s) 1410, 2710
BspMI ACCTGC 1 cut(s) 2871
BspPI GGATC 4 cut(s) 559, 881, 1423, 1944
BspQI GCTCTTC 1 cut(s) 1995
BspT104I TTCGAA 2 cut(s) 736, 2368
BspT107I GGYRCC 1 cut(s) 917
BsrBI CCGCTC 2 cut(s) 89, 2170
BsrDI GCAATG 4 cut(s) 567, 1247, 2164, 2972
BsrI ACTGG 4 cut(s) 808, 1204, 2699, 2881
BssECI CCNNGG 4 cut(s) 388, 845, 1466, 2022
BssMI GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
BssNI GRCGYC 1 cut(s) 918
BssT1I CCWWGG 3 cut(s) 388, 845, 2022
Bst2UI CCWGG 5 cut(s) 83, 1638, 1881, 2610, 2804
Bst4CI ACNGT 7 cut(s) 879, 1004, 1158, 1729, 2184, 2232, 2926
Bst6I CTCTTC 3 cut(s) 958, 1989, 1995
BstACI GRCGYC 1 cut(s) 918
BstAPI GCANNNNNTGC 1 cut(s) 2495
BstBI TTCGAA 2 cut(s) 736, 2368
BstC8I GCNNGC 5 cut(s) 411, 1090, 2349, 2559, 2630
BstH2I RGCGCY 2 cut(s) 921, 1111
BstHHI GCGC 3 cut(s) 262, 920, 1110
BstKTI GATC 7 cut(s) 397, 567, 889, 1418, 1543, 1939, 2226
BstMAI GTCTC 2 cut(s) 162, 1786
BstMBI GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
BstNI CCWGG 5 cut(s) 83, 1638, 1881, 2610, 2804
BstNSI RCATGY 1 cut(s) 2530
BstSCI CCNGG 9 cut(s) 81, 567, 1495, 1636, 1879, 1922, 2561, 2608, 2802
BstSFI CTRYAG 5 cut(s) 1406, 1659, 1719, 2706, 2922
BstSLI GKGCMC 1 cut(s) 1734
BstV2I GAAGAC 2 cut(s) 39, 816
BstX2I RGATCY 1 cut(s) 1415
BstYI RGATCY 1 cut(s) 1415
Bsu36I CCTNAGG 2 cut(s) 1114, 2385
BsuRI GGCC 7 cut(s) 65, 77, 86, 618, 1119, 1791, 1857
BtrI CACGTC 1 cut(s) 2164
BtsI GCAGTG 1 cut(s) 1965
BtsIMutI CAGTG 7 cut(s) 834, 882, 1009, 1734, 1965, 2180, 2874
BveI ACCTGC 1 cut(s) 2871
Cac8I GCNNGC 5 cut(s) 411, 1090, 2349, 2559, 2630
CaiI CAGNNNCTG 2 cut(s) 2705, 2930
CciI TCATGA 1 cut(s) 1270
CfoI GCGC 3 cut(s) 262, 920, 1110
Cfr13I GGNCC 9 cut(s) 4, 63, 309, 751, 1118, 1499, 2018, 2601, 2908
CseI GACGC 1 cut(s) 1771
CsiI ACCWGGT 2 cut(s) 1879, 2608
Csp6I GTAC 6 cut(s) 526, 608, 782, 1197, 1877, 2938
CviQI GTAC 6 cut(s) 526, 608, 782, 1197, 1877, 2938
DinI GGCGCC 1 cut(s) 919
DpnI GATC 7 cut(s) 396, 566, 888, 1417, 1542, 1938, 2225
DpnII GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
DraIII CACNNNGTG 2 cut(s) 1343, 2186
DriI GACNNNNNGTC 1 cut(s) 2530
EaeI YGGCCR 1 cut(s) 1855
Eam1104I CTCTTC 3 cut(s) 958, 1989, 1995
Eam1105I GACNNNNNGTC 1 cut(s) 2530
EarI CTCTTC 3 cut(s) 958, 1989, 1995
Eco130I CCWWGG 3 cut(s) 388, 845, 2022
Eco147I AGGCCT 1 cut(s) 77
Eco47I GGWCC 7 cut(s) 4, 309, 751, 1499, 2018, 2601, 2908
Eco47III AGCGCT 1 cut(s) 1109
Eco57I CTGAAG 5 cut(s) 521, 713, 810, 939, 2025
Eco81I CCTNAGG 2 cut(s) 1114, 2385
Eco88I CYCGRG 1 cut(s) 2502
EcoO109I RGGNCCY 2 cut(s) 1118, 2601
EcoRI GAATTC 1 cut(s) 1456
EcoRII CCWGG 5 cut(s) 81, 1636, 1879, 2608, 2802
EcoT14I CCWWGG 3 cut(s) 388, 845, 2022
EcoT22I ATGCAT 2 cut(s) 57, 279
EgeI GGCGCC 1 cut(s) 919
EheI GGCGCC 1 cut(s) 919
ErhI CCWWGG 3 cut(s) 388, 845, 2022
FalI AAGNNNNNCTT 4 cut(s) 381, 413, 2087, 2119
FaqI GGGAC 6 cut(s) 113, 764, 1240, 2220, 2587, 2728
FauI CCCGC 2 cut(s) 1011, 2235
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 2 cut(s) 1540, 2223
FblI GTMKAC 2 cut(s) 1402, 2831
FokI GGATG 9 cut(s) 56, 230, 353, 728, 862, 1162, 2035, 2534, 2801
FspBI CTAG 6 cut(s) 180, 846, 1077, 1398, 2325, 2991
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 3 cut(s) 261, 919, 1109
GsaI CCCAGC 3 cut(s) 646, 1818, 2715
GsuI CTGGAG 3 cut(s) 492, 2682, 2825
HaeII RGCGCY 2 cut(s) 921, 1111
HaeIII GGCC 7 cut(s) 65, 77, 86, 618, 1119, 1791, 1857
HapII CCGG 6 cut(s) 312, 569, 727, 1497, 1923, 2563
HgaI GACGC 1 cut(s) 1771
HhaI GCGC 3 cut(s) 262, 920, 1110
Hin1I GRCGYC 1 cut(s) 918
Hin6I GCGC 3 cut(s) 260, 918, 1108
HinP1I GCGC 3 cut(s) 260, 918, 1108
HincII GTYRAC 2 cut(s) 554, 2392
HindII GTYRAC 2 cut(s) 554, 2392
HindIII AAGCTT 2 cut(s) 533, 2363
HpaI GTTAAC 1 cut(s) 2392
HpaII CCGG 6 cut(s) 312, 569, 727, 1497, 1923, 2563
HphI GGTGA 6 cut(s) 404, 1205, 1717, 1881, 2420, 2878
HpyCH4III ACNGT 7 cut(s) 879, 1004, 1158, 1729, 2184, 2232, 2926
HpyCH4IV ACGT 3 cut(s) 495, 1030, 2163
HpySE526I ACGT 3 cut(s) 495, 1030, 2163
Hsp92I GRCGYC 1 cut(s) 918
HspAI GCGC 3 cut(s) 260, 918, 1108
KasI GGCGCC 1 cut(s) 917
KflI GGGWCCC 1 cut(s) 2601
Ksp22I TGATCA 2 cut(s) 1540, 2223
KspAI GTTAAC 1 cut(s) 2392
Kzo9I GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
LguI GCTCTTC 1 cut(s) 1995
LmnI GCTCC 9 cut(s) 30, 292, 571, 1105, 1115, 1486, 2104, 2167, 2438
LweI GCATC 8 cut(s) 331, 706, 747, 840, 2057, 2239, 2603, 2986
MabI ACCWGGT 2 cut(s) 1879, 2608
MaeI CTAG 6 cut(s) 180, 846, 1077, 1398, 2325, 2991
MaeII ACGT 3 cut(s) 495, 1030, 2163
MaeIII GTNAC 6 cut(s) 496, 621, 810, 1705, 1963, 2178
MalI GATC 7 cut(s) 396, 566, 888, 1417, 1542, 1938, 2225
MbiI CCGCTC 2 cut(s) 89, 2170
MboI GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
MfeI CAATTG 1 cut(s) 1536
MflI RGATCY 1 cut(s) 1415
MhlI GDGCHC 2 cut(s) 1734, 1834
MlsI TGGCCA 1 cut(s) 1857
MluNI TGGCCA 1 cut(s) 1857
Mly113I GGCGCC 1 cut(s) 918
MlyI GAGTC 3 cut(s) 137, 1034, 1664
MmeI TCCRAC 1 cut(s) 2494
Mox20I TGGCCA 1 cut(s) 1857
Mph1103I ATGCAT 2 cut(s) 57, 279
MroXI GAANNNNTTC 1 cut(s) 2364
MscI TGGCCA 1 cut(s) 1857
MseI TTAA 7 cut(s) 357, 530, 1134, 1983, 2376, 2391, 2445
MslI CAYNNNNRTG 2 cut(s) 282, 1358
Msp20I TGGCCA 1 cut(s) 1857
MspA1I CMGCKG 5 cut(s) 446, 2008, 2242, 2645, 2711
MspI CCGG 6 cut(s) 312, 569, 727, 1497, 1923, 2563
MspR9I CCNGG 9 cut(s) 83, 569, 1497, 1638, 1881, 1924, 2563, 2610, 2804
MunI CAATTG 1 cut(s) 1536
Mva1269I GAATGC 3 cut(s) 55, 277, 1056
MvaI CCWGG 5 cut(s) 83, 1638, 1881, 2610, 2804
NarI GGCGCC 1 cut(s) 918
NciI CCSGG 4 cut(s) 569, 1497, 1924, 2563
NdeI CATATG 1 cut(s) 283
NdeII GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
NmeAIII GCCGAG 1 cut(s) 1447
NmuCI GTSAC 3 cut(s) 1705, 1963, 2178
NsbI TGCGCA 1 cut(s) 261
NsiI ATGCAT 2 cut(s) 57, 279
NspI RCATGY 1 cut(s) 2530
NspV TTCGAA 2 cut(s) 736, 2368
OliI CACNNNNGTG 1 cut(s) 1358
PagI TCATGA 1 cut(s) 1270
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 2526
PciSI GCTCTTC 1 cut(s) 1995
PctI GAATGC 3 cut(s) 55, 277, 1056
PdmI GAANNNNTTC 1 cut(s) 2364
PfeI GAWTC 8 cut(s) 137, 518, 592, 977, 1274, 1492, 2308, 2859
PfoI TCCNGGA 3 cut(s) 567, 1922, 2802
PleI GAGTC 3 cut(s) 137, 1034, 1663
PluTI GGCGCC 1 cut(s) 921
PpsI GAGTC 3 cut(s) 137, 1034, 1663
PpuMI RGGWCCY 1 cut(s) 2601
PscI ACATGT 1 cut(s) 2526
Psp5II RGGWCCY 1 cut(s) 2601
Psp6I CCWGG 5 cut(s) 81, 1636, 1879, 2608, 2802
PspFI CCCAGC 3 cut(s) 642, 1814, 2711
PspGI CCWGG 5 cut(s) 81, 1636, 1879, 2608, 2802
PspPI GGNCC 9 cut(s) 4, 63, 309, 751, 1118, 1499, 2018, 2601, 2908
PspPPI RGGWCCY 1 cut(s) 2601
PstI CTGCAG 2 cut(s) 1410, 2710
PstNI CAGNNNCTG 2 cut(s) 2705, 2930
PsuI RGATCY 1 cut(s) 1415
PvuII CAGCTG 4 cut(s) 446, 2008, 2645, 2711
RsaI GTAC 6 cut(s) 527, 609, 783, 1198, 1878, 2939
RsaNI GTAC 6 cut(s) 526, 608, 782, 1197, 1877, 2938
RseI CAYNNNNRTG 2 cut(s) 282, 1358
SapI GCTCTTC 1 cut(s) 1995
SaqAI TTAA 7 cut(s) 357, 530, 1134, 1983, 2376, 2391, 2445
Sau3AI GATC 7 cut(s) 394, 564, 886, 1415, 1540, 1936, 2223
Sau96I GGNCC 9 cut(s) 4, 63, 309, 751, 1118, 1499, 2018, 2601, 2908
ScaI AGTACT 2 cut(s) 527, 1198
SchI GAGTC 3 cut(s) 137, 1034, 1664
ScrFI CCNGG 9 cut(s) 83, 569, 1497, 1638, 1881, 1924, 2563, 2610, 2804
SduI GDGCHC 2 cut(s) 1734, 1834
SexAI ACCWGGT 2 cut(s) 1879, 2608
SfaNI GCATC 8 cut(s) 331, 706, 747, 840, 2057, 2239, 2603, 2986
SfcI CTRYAG 5 cut(s) 1406, 1659, 1719, 2706, 2922
SfiI GGCCNNNNNGGCC 1 cut(s) 83
SfoI GGCGCC 1 cut(s) 919
SfuI TTCGAA 2 cut(s) 736, 2368
SinI GGWCC 7 cut(s) 4, 309, 751, 1499, 2018, 2601, 2908
SmiMI CAYNNNNRTG 2 cut(s) 282, 1358
SmlI CTYRAG 3 cut(s) 159, 1256, 1616
SmoI CTYRAG 3 cut(s) 159, 1256, 1616
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 9 cut(s) 87, 147, 194, 1018, 1082, 1895, 2170, 2242, 2626
SspDI GGCGCC 1 cut(s) 917
SspI AATATT 1 cut(s) 2998
SspMI CTAG 6 cut(s) 180, 846, 1077, 1398, 2325, 2991
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 9 cut(s) 81, 567, 1495, 1636, 1879, 1922, 2561, 2608, 2802
StyI CCWWGG 3 cut(s) 388, 845, 2022
TaaI ACNGT 7 cut(s) 879, 1004, 1158, 1729, 2184, 2232, 2926
TaiI ACGT 3 cut(s) 498, 1033, 2166
TaqI TCGA 6 cut(s) 736, 996, 1795, 2035, 2368, 2825
TatI WGTACW 3 cut(s) 525, 781, 1196
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 8 cut(s) 137, 518, 592, 977, 1274, 1492, 2308, 2859
Tru1I TTAA 7 cut(s) 357, 530, 1134, 1983, 2376, 2391, 2445
Tru9I TTAA 7 cut(s) 357, 530, 1134, 1983, 2376, 2391, 2445
TscAI CASTG 7 cut(s) 834, 889, 1009, 1734, 1972, 2187, 2881
TseFI GTSAC 3 cut(s) 1705, 1963, 2178
Tsp45I GTSAC 3 cut(s) 1705, 1963, 2178
TspGWI ACGGA 1 cut(s) 1023
TspRI CASTG 7 cut(s) 834, 889, 1009, 1734, 1972, 2187, 2881
VpaK11BI GGWCC 7 cut(s) 4, 309, 751, 1499, 2018, 2601, 2908
XapI RAATTY 9 cut(s) 353, 468, 1290, 1381, 1444, 1456, 1641, 1738, 2333
XceI RCATGY 1 cut(s) 2530
XmaJI CCTAGG 1 cut(s) 845
XmiI GTMKAC 2 cut(s) 1402, 2831
XmnI GAANNNNTTC 1 cut(s) 2364
XspI CTAG 6 cut(s) 180, 846, 1077, 1398, 2325, 2991
ZrmI AGTACT 2 cut(s) 527, 1198
Zsp2I ATGCAT 2 cut(s) 57, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.