pycom06g00540

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001817
Physical Location & Seq
Reverse (-)
507539 .. 516633
9095 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00540.4

Sequence Viewer

Length: 2838 bp
ATGGACCCCTCCATTATGAAGAAGCTGCTCGAAGACGATGAGGACGAAACCATGCATTCGGGGGCGGATGTGGAGGCCTTCCAGGCTGCCCTCAATAGGGACATTGAAGGAGATGCCTCTGCTTCCCAGCCATCTGATTCCGACTCCGCAATTTTGTCTCAAGGAAGCAATAATACTTCTAGTCAGCCATTACCACAGTTCCAAACAACTGGTCAGGATGAAAGTACAGCTTGCGAAATGCAACATGACCAAAAAATTGCACAACCGCAAGAACTGCATTCATATGAAATGGAGCTAAAGCAACAAGGATCGGTTGCTGAAAATATGCAGCTAAAAAGTGATGCCTCACAGGAACTTAGTCACTTTTCTTTGTCCCAGAAGCAACCCCAAGGTGATCTTCAACAAGGGCAAGCAGAACAAAAATCCCTCCAGGTACATGAAACAACTGGCACGCCGATTTCTGGAAAAATTCCCGTCTCTAAACAGGAGCAGGACATTACACCTACTCCACAAAGCGAATCTCAGTACTTAAAGCTTCAGAGGATGAGCAGTCAACAGTCAATGATCACTGAGCAGCCAAGCAACCCAATGAATCGTAGTAAACAAGTACCATTTGGCCTGTTACTACCTGTCCTATTGCCCCAGCTTGATAAGGACAGGGCCATGCAACTCAATACTCTATTTGGCAAACTCAAGAATAATGAAATCTCAAAAGACGCTTTTGTTCGGCACATTAGAAGTGTTGTTGGGGACCAGATGCTCAAATTGGCAGTAATGAAAGTACAATCGCAGCCAGGTCCTAAACACCAGTTACTGCCTCAGGCTTCAGTGCAGCAACAGTCTCCTAGGATGCCATCCGCTAGTGCAGGTTCCACACAGTTCGCTGATCCTCGTTCATTTGCACTTAATCAGAGAGGCACCAACTCTCCAACAGACCCATCGCACATTCCATCTTCAACAGTTCAACTGCAGTCTGATTCAAGCCATTCAGTCATTGAAAACAGTGCTAAAAAACTGCGGGAGGCAGAACGTCAGTCAGACTCTCATGGAATGCAAGTAAGCCAAATGTCTTCTTCTAGTGTGGTAGCTGGGAATCAAGAAAGGGAGCGCTCATCAGGTCCCATGCAAATACTTAATAAGCAGCAACAACAGTTGCAGTATCCACAATCATCCTATTCCATGTATGGAAGTACTGGTGGTAATTATCACCCATATACGGGGACAAGTATCAATACATTGCCTCTCAAACAGCAACCTCATGATTCACAACTTAGACAAATACCACAACATCAAAGCATGGGTTCAGCTCAGTCAGGTGGGGAAGCAAAGGGAGGGAACGTCATGGGTGTGTCCAATTTGGAGAGGCAAAATTTGATGAAGGACCCTAGTAGACTGCAGGGTGGATCTCTTTCGCACTTTACAAATAATTCAACTTTACAACAGAATATAGCTCCTTGGCAATCCTCAAATAAACAGGAACTGACTGATCAGACTGCTGAGCAGCAGCACAAGCCCCCATTATCTAATTCACAGGAATTGCCTTCTGCTGCGAAACTTGAGCAGGGGAATGGGTCACCTGGAATTTCAATGGACGAGCCGTTAGATAAGCAATCTTCAAGAATGGGGTTTCCAGCATCTGTAAGCATTGGGGCTTCGTCTTCTACAAGCACAGTGCCCCCGAATTCTGTTTCTTCTTCCATAACAACGCAAGCTGATCCTAATGTCTTGTTAGGCCATCGAATACCCTCTGGAACTTCTCCTGCTGGGATTAGTAATAGAGCACCTCCAAAAAAGGCTTCTGTTGGTCAAAAGAAGCCTCTTGAAGCACTTGGTTCTTCACCACCTCCGTCAAGTAAGAAGCAAAAGGTATCCGGGACCTTTTTGGATCAAAGCATTGAACAACTGAATGATGTGACTGCTGTTAGTGGAGTTAATCTTAGGGAAGAGGAAGAGCAGCTGTTTTCTGGCCCCAAGGAGGATAGTCGAGCTTCAGAAGCATCTCGAAAGTTTGTGCAAGAAGAAGAAGAGAGGCTGATTTTGCAGAAGGAACCCTTGCAGAAAAAGTTGGCAGAAATTATGATCAAATGTGGATTGAAAAGTGTAAGCAATGATGTGGAGCGGTGCTTGTCACTGTGTGTGGAGGAAAGAATGCGGGGACTAATAAATAATCTGATCAGACTGTCAAAGCAGCGAGTTGATGCTGAGAAGCCGAGACACCGCACTATTATTACCTCTGATGTTCGGCAGCAAGTTATGAATCTCAACCAGAATGCCAGGGAAGAATTTGAAAAAAAGCAAGCCGAGGCAGAAAAGCTTCGAAGGCTTAATGAACCTGAGGTTAGCAACGGAGTTGATGGTGATAAGGACAAAGATGAAGGTCGTTCGAAATCATTTAAGCCAAACAAAGAGGAAGATGACAAAATGAGGACAACAGCAGCAAATGTTGCTGCCCGAGCTGCTGTTGGAGGAGATGACATGTTGTCAAAATGGCAACTTATGGCTGAGCAAGCCCGGCAAAAACGTGAAGGTGGGATTGATGTAGCATCTGGGTCCCAGCCAGGTAAAGATGTAAACCGCAAGCCTACATTATCGGCTGGAAGAACTATGAAGGACAATCAAGAAGCAGAGAAAAGGAGCGGTGGAACTCCTGTTGCTTCAGCTGCTGGGACTATTAGAAAATGTGGAAAGAACCAAATTAACGTGCCTCAAACGAAGGTGGCTCGTAGTATATCTGTCAAGGATGTGATTGCTGTCCTAGAGAGAGAACCTCAGATGTCGAGATCTACTCTGATATATCGCTTGTTTGAGAGAGTTCAATCTAACACCACTGGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

946

Amino Acids

103.38

Weight (kDa)

7.02

Isoelectric Point (pI)

61.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 857
AccB1I GGYRCC 1 cut(s) 917
AccBSI CCGCTC 2 cut(s) 2119, 2637
AccI GTMKAC 1 cut(s) 1390
AclWI GGATC 5 cut(s) 316, 881, 1411, 1709, 1893
AcsI RAATTY 5 cut(s) 468, 1369, 1581, 1681, 2282
AcuI CTGAAG 4 cut(s) 521, 810, 1974, 2640
AdeI CACNNNGTG 1 cut(s) 2135
AfaI GTAC 6 cut(s) 226, 435, 527, 609, 783, 1192
AfeI AGCGCT 1 cut(s) 1109
AfiI CCNNNNNNNGG 6 cut(s) 96, 97, 461, 1216, 1217, 1975
AflIII ACRYGT 1 cut(s) 2475
AhdI GACNNNNNGTC 1 cut(s) 2479
AjnI CCWGG 6 cut(s) 81, 429, 793, 1576, 2273, 2557
AjuI GAANNNNNNNTTGG 4 cut(s) 381, 413, 1780, 1812
Alw21I GWGCWC 1 cut(s) 1783
Alw26I GTCTC 4 cut(s) 162, 481, 846, 2206
AlwI GGATC 5 cut(s) 316, 881, 1411, 1709, 1893
AlwNI CAGNNNCTG 4 cut(s) 814, 1480, 1637, 2663
Ama87I CYCGRG 1 cut(s) 2451
Aor51HI AGCGCT 1 cut(s) 1109
AoxI GGCC 5 cut(s) 75, 616, 660, 1732, 1966
ApoI RAATTY 5 cut(s) 468, 1369, 1581, 1681, 2282
ArsI GACNNNNNNTTYG 2 cut(s) 196, 228
Asp700I GAANNNNTTC 1 cut(s) 2313
AspA2I CCTAGG 1 cut(s) 845
AspLEI GCGC 1 cut(s) 1110
AspS9I GGNCC 9 cut(s) 4, 660, 751, 797, 1118, 1381, 1875, 1967, 2550
AsuC2I CCSGG 2 cut(s) 1873, 2512
AsuHPI GGTGA 5 cut(s) 404, 1199, 1566, 1830, 2369
AsuII TTCGAA 2 cut(s) 2317, 2384
AvaI CYCGRG 1 cut(s) 2451
AvaII GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1875, 2550
AvrII CCTAGG 1 cut(s) 845
AxyI CCTNAGG 2 cut(s) 819, 2334
BaeGI GKGCMC 1 cut(s) 1677
BanI GGYRCC 1 cut(s) 917
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BbsI GAAGAC 3 cut(s) 39, 1062, 1650
Bbv12I GWGCWC 1 cut(s) 1783
BccI CCATC 6 cut(s) 139, 862, 946, 958, 1743, 2348
BceAI ACGGC 1 cut(s) 1582
BciT130I CCWGG 6 cut(s) 83, 431, 795, 1578, 2275, 2559
BciVI GTATCC 2 cut(s) 1170, 1879
BclI TGATCA 4 cut(s) 564, 1486, 2079, 2172
BcnI CCSGG 2 cut(s) 1873, 2512
BcoDI GTCTC 4 cut(s) 162, 481, 846, 2206
BfaI CTAG 6 cut(s) 180, 846, 861, 1077, 1386, 2756
BfmI CTRYAG 2 cut(s) 968, 1394
BfoI RGCGCY 1 cut(s) 1111
BfuAI ACCTGC 1 cut(s) 857
BfuI GTATCC 2 cut(s) 1170, 1879
BglI GCCNNNNNGGC 1 cut(s) 83
BglII AGATCT 1 cut(s) 2780
BlnI CCTAGG 1 cut(s) 845
BlpI GCTNAGC 2 cut(s) 1497, 2502
BmcAI AGTACT 2 cut(s) 527, 1192
Bme1390I CCNGG 8 cut(s) 83, 431, 795, 1578, 1873, 2275, 2512, 2559
Bme18I GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1875, 2550
BmeRI GACNNNNNGTC 1 cut(s) 2479
BmeT110I CYCGRG 1 cut(s) 2451
BmgT120I GGNCC 9 cut(s) 4, 660, 751, 797, 1118, 1381, 1875, 1967, 2550
BmrFI CCNGG 8 cut(s) 83, 431, 795, 1578, 1873, 2275, 2512, 2559
BmsI GCATC 8 cut(s) 103, 331, 747, 840, 1643, 2006, 2188, 2552
BpiI GAAGAC 3 cut(s) 39, 1062, 1650
BplI GAGNNNNNCTC 4 cut(s) 2752, 2784, 2770, 2802
BpmI CTGGAG 1 cut(s) 413
Bpu1102I GCTNAGC 2 cut(s) 1497, 2502
Bpu14I TTCGAA 2 cut(s) 2317, 2384
BpuEI CTTGAG 3 cut(s) 144, 677, 1577
BpuMI CCSGG 2 cut(s) 1873, 2512
BsaJI CCNNGG 6 cut(s) 388, 845, 1454, 1971, 2274, 2301
BsaXI ACNNNNNCTCC 6 cut(s) 490, 520, 910, 940, 1323, 1353
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 97, 461, 1216, 1217, 1975
Bse1I ACTGG 5 cut(s) 214, 451, 808, 1198, 2833
Bse21I CCTNAGG 2 cut(s) 819, 2334
Bse3DI GCAATG 2 cut(s) 1235, 2113
BseBI CCWGG 6 cut(s) 83, 431, 795, 1578, 2275, 2559
BseDI CCNNGG 6 cut(s) 388, 845, 1454, 1971, 2274, 2301
BseGI GGATG 7 cut(s) 73, 223, 549, 854, 855, 1169, 2746
BseLI CCNNNNNNNGG 6 cut(s) 96, 97, 461, 1216, 1217, 1975
BseMI GCAATG 2 cut(s) 1235, 2113
BseMII CTCAG 9 cut(s) 536, 561, 833, 1322, 1488, 2193, 2325, 2493, 2783
BseNI ACTGG 5 cut(s) 214, 451, 808, 1198, 2833
BseRI GAGGAG 1 cut(s) 2481
BseSI GKGCMC 1 cut(s) 1677
BseYI CCCAGC 6 cut(s) 126, 642, 1088, 1763, 2553, 2663
BsgI GTGCAG 2 cut(s) 851, 885
BshFI GGCC 5 cut(s) 77, 618, 662, 1734, 1968
BshNI GGYRCC 1 cut(s) 917
BsiHKAI GWGCWC 1 cut(s) 1783
BsiHKCI CYCGRG 1 cut(s) 2451
BsiSI CCGG 2 cut(s) 1872, 2512
BslFI GGGAC 9 cut(s) 113, 358, 764, 1104, 1234, 1888, 2169, 2536, 2680
BslI CCNNNNNNNGG 6 cut(s) 96, 97, 461, 1216, 1217, 1975
BsmAI GTCTC 4 cut(s) 162, 481, 846, 2206
BsmBI CGTCTC 1 cut(s) 481
BsmFI GGGAC 9 cut(s) 113, 358, 764, 1104, 1234, 1888, 2169, 2536, 2680
BsmI GAATGC 5 cut(s) 55, 277, 1056, 2154, 2275
BsnI GGCC 5 cut(s) 77, 618, 662, 1734, 1968
BsoBI CYCGRG 1 cut(s) 2451
Bsp119I TTCGAA 2 cut(s) 2317, 2384
Bsp1286I GDGCHC 2 cut(s) 1677, 1783
Bsp1720I GCTNAGC 2 cut(s) 1497, 2502
BspANI GGCC 5 cut(s) 77, 618, 662, 1734, 1968
BspCNI CTCAG 9 cut(s) 535, 562, 832, 1321, 1489, 2194, 2326, 2494, 2782
BspHI TCATGA 1 cut(s) 1258
BspMAI CTGCAG 2 cut(s) 972, 1398
BspMI ACCTGC 1 cut(s) 857
BspPI GGATC 5 cut(s) 316, 881, 1411, 1709, 1893
BspQI GCTCTTC 1 cut(s) 1944
BspT104I TTCGAA 2 cut(s) 2317, 2384
BspT107I GGYRCC 1 cut(s) 917
BsrBI CCGCTC 2 cut(s) 2119, 2637
BsrDI GCAATG 2 cut(s) 1235, 2113
BsrI ACTGG 5 cut(s) 214, 451, 808, 1198, 2833
BssECI CCNNGG 6 cut(s) 388, 845, 1454, 1971, 2274, 2301
BssT1I CCWWGG 4 cut(s) 388, 845, 1454, 1971
Bst2UI CCWGG 6 cut(s) 83, 431, 795, 1578, 2275, 2559
Bst6I CTCTTC 3 cut(s) 1938, 1944, 2019
BstAPI GCANNNNNTGC 2 cut(s) 274, 2444
BstBI TTCGAA 2 cut(s) 2317, 2384
BstC8I GCNNGC 7 cut(s) 232, 411, 452, 1710, 2298, 2508, 2579
BstEII GGTNACC 1 cut(s) 1572
BstF5I GGATG 7 cut(s) 73, 223, 549, 854, 855, 1169, 2746
BstH2I RGCGCY 1 cut(s) 1111
BstHHI GCGC 1 cut(s) 1110
BstMAI GTCTC 4 cut(s) 162, 481, 846, 2206
BstNI CCWGG 6 cut(s) 83, 431, 795, 1578, 2275, 2559
BstNSI RCATGY 1 cut(s) 2479
BstPI GGTNACC 1 cut(s) 1572
BstSCI CCNGG 8 cut(s) 81, 429, 793, 1576, 1871, 2273, 2510, 2557
BstSFI CTRYAG 2 cut(s) 968, 1394
BstSLI GKGCMC 1 cut(s) 1677
BstV2I GAAGAC 3 cut(s) 39, 1062, 1650
BstX2I RGATCY 2 cut(s) 1403, 2780
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 2 cut(s) 1403, 2780
Bsu36I CCTNAGG 2 cut(s) 819, 2334
BsuI GTATCC 2 cut(s) 1170, 1879
BsuRI GGCC 5 cut(s) 77, 618, 662, 1734, 1968
BtgZI GCGATG 1 cut(s) 924
BtsCI GGATG 7 cut(s) 73, 223, 549, 854, 855, 1169, 2746
BtsIMutI CAGTG 6 cut(s) 567, 834, 1009, 1677, 2129, 2826
BveI ACCTGC 1 cut(s) 857
Cac8I GCNNGC 7 cut(s) 232, 411, 452, 1710, 2298, 2508, 2579
CaiI CAGNNNCTG 4 cut(s) 814, 1480, 1637, 2663
CciI TCATGA 1 cut(s) 1258
CfoI GCGC 1 cut(s) 1110
Cfr13I GGNCC 9 cut(s) 4, 660, 751, 797, 1118, 1381, 1875, 1967, 2550
CseI GACGC 1 cut(s) 725
Csp6I GTAC 6 cut(s) 225, 434, 526, 608, 782, 1191
CviQI GTAC 6 cut(s) 225, 434, 526, 608, 782, 1191
DraIII CACNNNGTG 1 cut(s) 2135
DriI GACNNNNNGTC 1 cut(s) 2479
Eam1104I CTCTTC 3 cut(s) 1938, 1944, 2019
Eam1105I GACNNNNNGTC 1 cut(s) 2479
EarI CTCTTC 3 cut(s) 1938, 1944, 2019
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 4 cut(s) 388, 845, 1454, 1971
Eco147I AGGCCT 1 cut(s) 77
Eco47I GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1875, 2550
Eco47III AGCGCT 1 cut(s) 1109
Eco57I CTGAAG 4 cut(s) 521, 810, 1974, 2640
Eco81I CCTNAGG 2 cut(s) 819, 2334
Eco88I CYCGRG 1 cut(s) 2451
Eco91I GGTNACC 1 cut(s) 1572
EcoO109I RGGNCCY 5 cut(s) 797, 1118, 1381, 1875, 2550
EcoO65I GGTNACC 1 cut(s) 1572
EcoRI GAATTC 1 cut(s) 1681
EcoRII CCWGG 6 cut(s) 81, 429, 793, 1576, 2273, 2557
EcoT14I CCWWGG 4 cut(s) 388, 845, 1454, 1971
EcoT22I ATGCAT 1 cut(s) 57
ErhI CCWWGG 4 cut(s) 388, 845, 1454, 1971
Esp3I CGTCTC 1 cut(s) 481
FalI AAGNNNNNCTT 6 cut(s) 214, 246, 381, 413, 2036, 2068
FaqI GGGAC 9 cut(s) 113, 358, 764, 1104, 1234, 1888, 2169, 2536, 2680
FauI CCCGC 2 cut(s) 1011, 2145
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 4 cut(s) 564, 1486, 2079, 2172
FblI GTMKAC 1 cut(s) 1390
FokI GGATG 7 cut(s) 80, 230, 556, 841, 862, 1156, 2753
FspBI CTAG 6 cut(s) 180, 846, 861, 1077, 1386, 2756
GlaI GCGC 1 cut(s) 1109
GsaI CCCAGC 6 cut(s) 130, 646, 1092, 1767, 2557, 2667
GsuI CTGGAG 1 cut(s) 413
HaeII RGCGCY 1 cut(s) 1111
HaeIII GGCC 5 cut(s) 77, 618, 662, 1734, 1968
HapII CCGG 2 cut(s) 1872, 2512
HgaI GACGC 1 cut(s) 725
HhaI GCGC 1 cut(s) 1110
Hin6I GCGC 1 cut(s) 1108
HinP1I GCGC 1 cut(s) 1108
HincII GTYRAC 1 cut(s) 554
HindII GTYRAC 1 cut(s) 554
HindIII AAGCTT 2 cut(s) 533, 2312
HinfI GANTC 9 cut(s) 137, 143, 518, 592, 977, 1040, 1093, 1262, 2257
HpaII CCGG 2 cut(s) 1872, 2512
HphI GGTGA 5 cut(s) 404, 1199, 1566, 1830, 2369
Hpy166II GTNNAC 4 cut(s) 554, 602, 1391, 2572
Hpy8I GTNNAC 4 cut(s) 554, 602, 1391, 2572
HpyCH4IV ACGT 4 cut(s) 1030, 1338, 2521, 2700
HpySE526I ACGT 4 cut(s) 1030, 1338, 2521, 2700
HspAI GCGC 1 cut(s) 1108
KflI GGGWCCC 1 cut(s) 2550
Ksp22I TGATCA 4 cut(s) 564, 1486, 2079, 2172
LguI GCTCTTC 1 cut(s) 1944
LmnI GCTCC 6 cut(s) 292, 487, 1105, 1456, 2116, 2634
LweI GCATC 8 cut(s) 103, 331, 747, 840, 1643, 2006, 2188, 2552
MaeI CTAG 6 cut(s) 180, 846, 861, 1077, 1386, 2756
MaeII ACGT 4 cut(s) 1030, 1338, 2521, 2700
MaeIII GTNAC 6 cut(s) 359, 621, 810, 1572, 1912, 2127
MbiI CCGCTC 2 cut(s) 2119, 2637
MflI RGATCY 2 cut(s) 1403, 2780
MhlI GDGCHC 2 cut(s) 1677, 1783
MlyI GAGTC 2 cut(s) 137, 1034
MmeI TCCRAC 3 cut(s) 165, 953, 2443
Mph1103I ATGCAT 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 2313
MseI TTAA 7 cut(s) 530, 906, 1134, 1932, 2325, 2394, 2697
MslI CAYNNNNRTG 2 cut(s) 282, 1346
MspA1I CMGCKG 2 cut(s) 1957, 2660
MspI CCGG 2 cut(s) 1872, 2512
MspR9I CCNGG 8 cut(s) 83, 431, 795, 1578, 1873, 2275, 2512, 2559
Mva1269I GAATGC 5 cut(s) 55, 277, 1056, 2154, 2275
MvaI CCWGG 6 cut(s) 83, 431, 795, 1578, 2275, 2559
NciI CCSGG 2 cut(s) 1873, 2512
NdeI CATATG 1 cut(s) 283
NmeAIII GCCGAG 2 cut(s) 2235, 2326
NmuCI GTSAC 4 cut(s) 359, 1572, 1912, 2127
NsiI ATGCAT 1 cut(s) 57
NspI RCATGY 1 cut(s) 2479
NspV TTCGAA 2 cut(s) 2317, 2384
PagI TCATGA 1 cut(s) 1258
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 2475
PciSI GCTCTTC 1 cut(s) 1944
PcsI WCGNNNNNNNCGW 1 cut(s) 42
PctI GAATGC 5 cut(s) 55, 277, 1056, 2154, 2275
PdmI GAANNNNTTC 1 cut(s) 2313
PfeI GAWTC 7 cut(s) 137, 518, 592, 977, 1093, 1262, 2257
PfoI TCCNGGA 1 cut(s) 1871
PleI GAGTC 2 cut(s) 137, 1034
PpsI GAGTC 2 cut(s) 137, 1034
PpuMI RGGWCCY 5 cut(s) 797, 1118, 1381, 1875, 2550
PscI ACATGT 1 cut(s) 2475
Psp5II RGGWCCY 5 cut(s) 797, 1118, 1381, 1875, 2550
Psp6I CCWGG 6 cut(s) 81, 429, 793, 1576, 2273, 2557
PspEI GGTNACC 1 cut(s) 1572
PspFI CCCAGC 6 cut(s) 126, 642, 1088, 1763, 2553, 2663
PspGI CCWGG 6 cut(s) 81, 429, 793, 1576, 2273, 2557
PspPI GGNCC 9 cut(s) 4, 660, 751, 797, 1118, 1381, 1875, 1967, 2550
PspPPI RGGWCCY 5 cut(s) 797, 1118, 1381, 1875, 2550
PstI CTGCAG 2 cut(s) 972, 1398
PstNI CAGNNNCTG 4 cut(s) 814, 1480, 1637, 2663
PsuI RGATCY 2 cut(s) 1403, 2780
PvuII CAGCTG 2 cut(s) 1957, 2660
RsaI GTAC 6 cut(s) 226, 435, 527, 609, 783, 1192
RsaNI GTAC 6 cut(s) 225, 434, 526, 608, 782, 1191
RseI CAYNNNNRTG 2 cut(s) 282, 1346
SapI GCTCTTC 1 cut(s) 1944
SaqAI TTAA 7 cut(s) 530, 906, 1134, 1932, 2325, 2394, 2697
Sau96I GGNCC 9 cut(s) 4, 660, 751, 797, 1118, 1381, 1875, 1967, 2550
ScaI AGTACT 2 cut(s) 527, 1192
SchI GAGTC 2 cut(s) 137, 1034
ScrFI CCNGG 8 cut(s) 83, 431, 795, 1578, 1873, 2275, 2512, 2559
SduI GDGCHC 2 cut(s) 1677, 1783
SfaNI GCATC 8 cut(s) 103, 331, 747, 840, 1643, 2006, 2188, 2552
SfcI CTRYAG 2 cut(s) 968, 1394
SfuI TTCGAA 2 cut(s) 2317, 2384
SinI GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1875, 2550
SmiMI CAYNNNNRTG 2 cut(s) 282, 1346
SmlI CTYRAG 3 cut(s) 159, 692, 1556
SmoI CTYRAG 3 cut(s) 159, 692, 1556
SseBI AGGCCT 1 cut(s) 77
SspMI CTAG 6 cut(s) 180, 846, 861, 1077, 1386, 2756
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 8 cut(s) 81, 429, 793, 1576, 1871, 2273, 2510, 2557
StyI CCWWGG 4 cut(s) 388, 845, 1454, 1971
TaiI ACGT 4 cut(s) 1033, 1341, 2524, 2703
TaqI TCGA 7 cut(s) 30, 1738, 1984, 2002, 2317, 2384, 2777
TatI WGTACW 4 cut(s) 224, 525, 781, 1190
TfiI GAWTC 7 cut(s) 137, 518, 592, 977, 1093, 1262, 2257
Tru1I TTAA 7 cut(s) 530, 906, 1134, 1932, 2325, 2394, 2697
Tru9I TTAA 7 cut(s) 530, 906, 1134, 1932, 2325, 2394, 2697
TscAI CASTG 6 cut(s) 574, 834, 1009, 1677, 2136, 2833
TseFI GTSAC 4 cut(s) 359, 1572, 1912, 2127
Tsp45I GTSAC 4 cut(s) 359, 1572, 1912, 2127
TspGWI ACGGA 2 cut(s) 1836, 2361
TspRI CASTG 6 cut(s) 574, 834, 1009, 1677, 2136, 2833
VpaK11BI GGWCC 7 cut(s) 4, 751, 797, 1118, 1381, 1875, 2550
XapI RAATTY 5 cut(s) 468, 1369, 1581, 1681, 2282
XceI RCATGY 1 cut(s) 2479
XmaJI CCTAGG 1 cut(s) 845
XmiI GTMKAC 1 cut(s) 1390
XmnI GAANNNNTTC 1 cut(s) 2313
XspI CTAG 6 cut(s) 180, 846, 861, 1077, 1386, 2756
ZrmI AGTACT 2 cut(s) 527, 1192
Zsp2I ATGCAT 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.