Rw7G025260

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001817
Physical Location & Seq
Forward (+)
31608754 .. 31617062
8309 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025260.1

Sequence Viewer

Length: 2871 bp
ATGGACCCGTCGATTATGAAGAAGCTCTTGGAAGACGATGAGGATGAAACAATGCATTCGGGGGCGGATGTGGAGGCCTTTCAGGCGGCCCTGAATAGGGACATTGAAGGAGATGTGTCTGCTTCTCAGCGGTCTGATTCAGACTCTGCGGTTTTGTCTCAAGGAAGCAATAATACTTCTAGTCAATCATTACCGCAGTTGCATACTGCTAGCCAGGATGAAAGTACTGCTGGTCAAATTCAGCATGACCAAAATATTGCACAACAGCGAGAAATGCAGTCATATGAAAGGGAGTTAAAGCCACAACGTTCCGTCTCTGAGAATATGCCACAACAGAATGATGCATCGCAGGAACTTAATCATTTTACTTTGCCCCAGAAGCAACCCCACAGTGATCTTCAGCAAGGGAAAGGAGATCAAAAACCTCTCCAGACTGGAATGTTGATGTCTGGAAAAAATCCAGTGTCAACACAGGAGCAGGACTTAACTCCTAAACCAGAAAATGAATCTCAGTATGTAAAGCTTCAGAAGATGAGTAGTCAACAGGCAATGACCACGGAGCAGCCAAGCACCCCGATGAATCGCAGTAAACAAGTACCATTTGGTCTGTTACTACCTGTCTTATTGCCTCAACTTGATAAAGACAGAGCCATGCAACTTACAACTCTTTTTGGTAAACTGAAGAACAATGAAATCTCCAAAGATGCTTTTGTCCGACACATTAGAAGTGTTGTTGGGGACCAGATGCTCAAGATGGCAGTACATAAAGTACAGACACAGCCTGCTCTTAAACAACAGTTGATGCCTCAGGCTTCGTTGCAGCAACAACCTCCTAGGATGCCATCTATTAGTGCAGGTGCTACCCAGTTCACTGATCCTCGTTTTGCAATTCATCAGAGAGGTGCGAACCCTTCCACAGACCCATCGCACATTCCCACAGTGCACGTGCAGACTGATTCAAGCCATTCGGCCATTGAAAACAATGCCAAAAAATTGCGGGAAGCAGAACGTCAGTCAGATTCTCATGCAATGCAAGTAAATCAGATGTCTTCTTCCAGCACAGGAGCTGCCAATCAAGAAAGGGAGCGGTCCTCGGTTGCTATGCAAGTACATAACAAGCAGCAGCAACAGCAACTGCACTACCCCCAATCATCCTTTGCCATGTATGGATCTACTGGTGGTAATTATCGTCCATATTCAGGGACAAATGTCAATACAATGCCTCTCAAACAGCAATCACATGACTCTCATTTGAGGCAAATTCCTCAACATCAAAGCATGGGCTCAGCTCAGCCAGGTGGGGTGGAAGCCCAGGGAACAAACATCATGAGTGTGCCCAAATTGGAGGGGCAAAATTCTATGAATGACCCTAGCAGACAGCAGGGTGGATCTCTTCCTCACTTCACAAATAATTCGACTTTACAACAAAATCCAGTTCCTTGGCAGTCTTCAAATAAAGAGCAGATTTCTGGCCCTTTATCATCAATGCCTTATGTAAAACAGGAACCCAGTGACCAGAGTGCTGAGCAGCAGCACAAGACCCCACTATCTAATTCTCAGCGTTTGCCTTATACTTCTTCTGTGCAACATGAACAAGGAAATGCATCACCTGGAATTTCAATGGACGAGTCTTTAGAGAAGCAGTCTTCAAGAATGGGATTTTCAAACACAGGGCCCTCTGGGAGCATGGTGCCTTCGTCATCTACAAACACAGGGCCCTTGAATCCTGTTTCTTCTTCCACAACGACGCAAGCTGATCCTAACTTAAGCTCTCGGATACCTACTGGCACTTCTCCTGCTGGGATTAATAATAGAGTAGCTGCAAAAAAGACTTCTGTTGGCCAAAAGAAGCCTAGTGAAGCACTTGGTTCTTCACCACCGCCTTCAAGTAAGAAGCAAAAAGTATCTGGGGCTTTTTTGGATCAAAGCATTGAACAACTCAATGATGTCACAGCTGTTAGTGGAGTTAATCTAAGGGAAGAGGAGGAGCAGCTGTTTTCTGGGCCCAAGGATGACGGTCGAGCTTCAGAAGCATCTCGAAGAGTTGTGCAAGAAGAAGAAGAAAGGCTGATTTTGCAGAAGGCTCCCTTGCAGAAAAAATTGGCAGAAATCATGTTCAGATGTGGTTTGAAAAGTATAAGCAATGATGTGGAACGTTGCTTGTCACTGTGCGTGGAGGAAAGAATGAGGGGACTGATAAATAATTTGATAAGACTGTCAAAGCAGCGGGTTGATGCTGAGAAACCAAAACACCACACCATCATTACCTCTGATGTTCGGCAACAAATTTTAAATCAGAACAAGAAAGCTAAGGAAGAATGGGAAAAGAAGCAAGCTGAGGCAGAAAAGGTCCGGAAGCTTAATGAACCTGAGGTTAGCAATGGAGTTGACGGTGATAAAGACAGAGATGAAGGTCGTTCTAAATCATTTAAGGTAAACAAAGAAGAAGATGACAAAATGAGGACAACAGCAGCAAATGTTGCTGCCCGTGCTGCTGTTGGAGGAGATGACATGTTGTCAAAATGGCAACTTATGGCTGAGCAAGCTCGCCAAAAACGTGAAGGTGGGAGTGATGTGGCATCTGGGTCTCAGCCAGGTAAAGATGTAAATCGCAAGCCTACATCAGCAGCTGGAAGAATTATGAAGGACAATCAAGAAGTAGAAAAAAGAGGGGGTGCAGCTCCTGTTTCTGCAGCTGGGACTGTTAGAAAATTTGGGAAAAACCAATTTATTGTGCCCCAGACAAGGGTGGCTCGTAGTATTTCTATCAAGGATGTGATTTCTGTTCTGGAAAGGGAACCTCAAATGTCCAAGTCACCTCTGATATATCGCTTGTATGAGAGAAACCAATCTGACTCTGCCGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

956

Amino Acids

105.12

Weight (kDa)

8.51

Isoelectric Point (pI)

62.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 194 - 258 4.6e-20 RCD1-SRO-TAF4 (RST) plant domain
TAF4 PF05236 647 - 945 2.6e-82 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 845
Acc36I ACCTGC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 1690
AccBSI CCGCTC 1 cut(s) 1087
AccIII TCCGGA 1 cut(s) 2352
AciI CCGC 9 cut(s) 65, 86, 130, 149, 194, 997, 1087, 1880, 2227
AclI AACGTT 2 cut(s) 307, 2155
AclWI GGATC 5 cut(s) 869, 1177, 1396, 1751, 1929
AcoI YGGCCR 2 cut(s) 969, 1840
AcsI RAATTY 6 cut(s) 237, 1260, 1354, 1614, 2286, 2711
AcuI CTGAAG 4 cut(s) 383, 509, 701, 2010
AcvI CACGTG 1 cut(s) 946
AfaI GTAC 5 cut(s) 226, 597, 762, 771, 1110
AfiI CCNNNNNNNGG 5 cut(s) 96, 97, 1199, 2745, 2746
AflII CTTAAG 1 cut(s) 1765
AflIII ACRYGT 1 cut(s) 2511
AhdI GACNNNNNGTC 1 cut(s) 2515
AjnI CCWGG 5 cut(s) 213, 1294, 1311, 1609, 2593
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
Alw21I GWGCWC 1 cut(s) 945
Alw26I GTCTC 3 cut(s) 162, 319, 2592
Alw44I GTGCAC 1 cut(s) 941
AlwI GGATC 5 cut(s) 869, 1177, 1396, 1751, 1929
AlwNI CAGNNNCTG 5 cut(s) 146, 1067, 1135, 2630, 2684
Aor13HI TCCGGA 1 cut(s) 2352
AoxI GGCC 8 cut(s) 75, 87, 969, 1471, 1673, 1715, 1840, 2003
ApaI GGGCCC 3 cut(s) 1677, 1719, 2007
ApaLI GTGCAC 1 cut(s) 941
ApoI RAATTY 6 cut(s) 237, 1260, 1354, 1614, 2286, 2711
AseI ATTAAT 1 cut(s) 1806
AspA2I CCTAGG 1 cut(s) 833
AsuHPI GGTGA 4 cut(s) 1599, 1866, 2405, 2808
AsuNHI GCTAGC 1 cut(s) 209
AvaII GGWCC 4 cut(s) 4, 739, 1089, 2350
AvrII CCTAGG 1 cut(s) 833
AxyI CCTNAGG 2 cut(s) 807, 2370
BaeGI GKGCMC 6 cut(s) 945, 1338, 1677, 1719, 2007, 2739
BalI TGGCCA 1 cut(s) 1842
BanI GGYRCC 1 cut(s) 1690
BanII GRGCYC 4 cut(s) 1286, 1677, 1719, 2007
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BbrPI CACGTG 1 cut(s) 946
BbsI GAAGAC 4 cut(s) 39, 1041, 1440, 1638
Bbv12I GWGCWC 1 cut(s) 945
BbvCI CCTCAGC 1 cut(s) 2337
BccI CCATC 4 cut(s) 748, 850, 931, 2267
BciT130I CCWGG 5 cut(s) 215, 1296, 1313, 1611, 2595
BciVI GTATCC 1 cut(s) 1770
BcoDI GTCTC 3 cut(s) 162, 319, 2592
BfaI CTAG 5 cut(s) 180, 210, 834, 1371, 1854
BfmI CTRYAG 1 cut(s) 2691
BfrI CTTAAG 1 cut(s) 1765
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 1770
BglI GCCNNNNNGGC 1 cut(s) 83
BlnI CCTAGG 1 cut(s) 833
BlpI GCTNAGC 4 cut(s) 1285, 1290, 1524, 2538
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 5 cut(s) 215, 1296, 1313, 1611, 2595
Bme18I GGWCC 4 cut(s) 4, 739, 1089, 2350
BmeRI GACNNNNNGTC 1 cut(s) 2515
BmiI GGNNCC 9 cut(s) 6, 740, 1506, 1675, 1692, 1717, 2005, 2085, 2799
BmrFI CCNGG 5 cut(s) 215, 1296, 1313, 1611, 2595
BmrI ACTGGG 2 cut(s) 859, 1503
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 2 cut(s) 859, 1503
BoxI GACNNNNGTC 1 cut(s) 1208
BpiI GAAGAC 4 cut(s) 39, 1041, 1440, 1638
BplI GAGNNNNNCTC 2 cut(s) 1076, 1108
BpmI CTGGAG 1 cut(s) 413
Bpu10I CCTNAGC 2 cut(s) 2310, 2337
Bpu1102I GCTNAGC 4 cut(s) 1285, 1290, 1524, 2538
BpuEI CTTGAG 2 cut(s) 144, 734
BsaAI YACGTR 1 cut(s) 946
BsaBI GATNNNNATC 1 cut(s) 2607
BsaI GGTCTC 1 cut(s) 2592
BsaJI CCNNGG 7 cut(s) 555, 833, 1092, 1311, 1312, 1439, 2007
BsaWI WCCGGW 1 cut(s) 2352
BsaXI ACNNNNNCTCC 2 cut(s) 1979, 2009
Bsc4I CCNNNNNNNGG 5 cut(s) 96, 97, 1199, 2745, 2746
Bse118I RCCGGY 1 cut(s) 2861
Bse1I ACTGG 7 cut(s) 439, 461, 865, 1180, 1433, 1509, 1789
Bse21I CCTNAGG 2 cut(s) 807, 2370
Bse3DI GCAATG 4 cut(s) 555, 1035, 2149, 2386
Bse8I GATNNNNATC 1 cut(s) 2607
BseAI TCCGGA 1 cut(s) 2352
BseBI CCWGG 5 cut(s) 215, 1296, 1313, 1611, 2595
BseDI CCNNGG 7 cut(s) 555, 833, 1092, 1311, 1312, 1439, 2007
BseGI GGATG 7 cut(s) 49, 73, 223, 843, 1151, 2017, 2779
BseJI GATNNNNATC 1 cut(s) 2607
BseLI CCNNNNNNNGG 5 cut(s) 96, 97, 1199, 2745, 2746
BseMI GCAATG 4 cut(s) 555, 1035, 2149, 2386
BseNI ACTGG 7 cut(s) 439, 461, 865, 1180, 1433, 1509, 1789
BseRI GAGGAG 3 cut(s) 1997, 2000, 2517
BseSI GKGCMC 6 cut(s) 945, 1338, 1677, 1719, 2007, 2739
BseYI CCCAGC 2 cut(s) 1799, 2696
BsgI GTGCAG 4 cut(s) 873, 968, 1121, 2697
Bsh1285I CGRYCG 1 cut(s) 2020
BshFI GGCC 8 cut(s) 77, 89, 971, 1473, 1675, 1717, 1842, 2005
BshNI GGYRCC 1 cut(s) 1690
BsiEI CGRYCG 1 cut(s) 2020
BsiHKAI GWGCWC 1 cut(s) 945
BsiSI CCGG 2 cut(s) 2353, 2862
BslFI GGGAC 5 cut(s) 113, 752, 1216, 2205, 2713
BslI CCNNNNNNNGG 5 cut(s) 96, 97, 1199, 2745, 2746
BsmAI GTCTC 3 cut(s) 162, 319, 2592
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 5 cut(s) 113, 752, 1216, 2205, 2713
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 8 cut(s) 77, 89, 971, 1473, 1675, 1717, 1842, 2005
Bso31I GGTCTC 1 cut(s) 2592
Bsp120I GGGCCC 3 cut(s) 1673, 1715, 2003
Bsp1286I GDGCHC 7 cut(s) 945, 1286, 1338, 1677, 1719, 2007, 2739
Bsp13I TCCGGA 1 cut(s) 2352
Bsp143I GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
Bsp1720I GCTNAGC 4 cut(s) 1285, 1290, 1524, 2538
BspACI CCGC 9 cut(s) 65, 86, 130, 149, 194, 997, 1087, 1880, 2227
BspANI GGCC 8 cut(s) 77, 89, 971, 1473, 1675, 1717, 1842, 2005
BspEI TCCGGA 1 cut(s) 2352
BspHI TCATGA 1 cut(s) 1326
BspLI GGNNCC 9 cut(s) 6, 740, 1506, 1675, 1692, 1717, 2005, 2085, 2799
BspMAI CTGCAG 1 cut(s) 2695
BspMI ACCTGC 1 cut(s) 845
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 5 cut(s) 869, 1177, 1396, 1751, 1929
BspT107I GGYRCC 1 cut(s) 1690
BspTI CTTAAG 1 cut(s) 1765
BspTNI GGTCTC 1 cut(s) 2592
BsrBI CCGCTC 1 cut(s) 1087
BsrDI GCAATG 4 cut(s) 555, 1035, 2149, 2386
BsrFI RCCGGY 1 cut(s) 2861
BsrI ACTGG 7 cut(s) 439, 461, 865, 1180, 1433, 1509, 1789
BssAI RCCGGY 1 cut(s) 2861
BssECI CCNNGG 7 cut(s) 555, 833, 1092, 1311, 1312, 1439, 2007
BssMI GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
BssT1I CCWWGG 3 cut(s) 833, 1439, 2007
Bst2UI CCWGG 5 cut(s) 215, 1296, 1313, 1611, 2595
Bst4CI ACNGT 8 cut(s) 392, 798, 940, 2018, 2169, 2217, 2393, 2704
Bst6I CTCTTC 3 cut(s) 1398, 1974, 2035
BstAFI CTTAAG 1 cut(s) 1765
BstAPI GCANNNNNTGC 1 cut(s) 2480
BstBAI YACGTR 1 cut(s) 946
BstC8I GCNNGC 7 cut(s) 211, 783, 1752, 2334, 2544, 2548, 2615
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 7 cut(s) 49, 73, 223, 843, 1151, 2017, 2779
BstKTI GATC 7 cut(s) 397, 418, 877, 1172, 1391, 1759, 1924
BstMAI GTCTC 3 cut(s) 162, 319, 2592
BstMBI GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
BstMCI CGRYCG 1 cut(s) 2020
BstNI CCWGG 5 cut(s) 215, 1296, 1313, 1611, 2595
BstNSI RCATGY 1 cut(s) 2515
BstPAI GACNNNNGTC 1 cut(s) 1208
BstSCI CCNGG 5 cut(s) 213, 1294, 1311, 1609, 2593
BstSFI CTRYAG 1 cut(s) 2691
BstSLI GKGCMC 6 cut(s) 945, 1338, 1677, 1719, 2007, 2739
BstV2I GAAGAC 4 cut(s) 39, 1041, 1440, 1638
BstX2I RGATCY 2 cut(s) 1169, 1388
BstXI CCANNNNNNTGG 1 cut(s) 1440
BstYI RGATCY 2 cut(s) 1169, 1388
Bsu36I CCTNAGG 2 cut(s) 807, 2370
BsuI GTATCC 1 cut(s) 1770
BsuRI GGCC 8 cut(s) 77, 89, 971, 1473, 1675, 1717, 1842, 2005
BtgI CCRYGG 1 cut(s) 555
BtgZI GCGATG 2 cut(s) 330, 909
BtsCI GGATG 7 cut(s) 49, 73, 223, 843, 1151, 2017, 2779
BtsIMutI CAGTG 6 cut(s) 397, 468, 870, 945, 1516, 2165
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 7 cut(s) 211, 783, 1752, 2334, 2544, 2548, 2615
CaiI CAGNNNCTG 5 cut(s) 146, 1067, 1135, 2630, 2684
CciI TCATGA 1 cut(s) 1326
Cfr10I RCCGGY 1 cut(s) 2861
CseI GACGC 1 cut(s) 1756
Csp6I GTAC 5 cut(s) 225, 596, 761, 770, 1109
CviQI GTAC 5 cut(s) 225, 596, 761, 770, 1109
DpnI GATC 7 cut(s) 396, 417, 876, 1171, 1390, 1758, 1923
DpnII GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
DraI TTTAAA 1 cut(s) 2292
DriI GACNNNNNGTC 1 cut(s) 2515
EaeI YGGCCR 2 cut(s) 969, 1840
Eam1104I CTCTTC 3 cut(s) 1398, 1974, 2035
Eam1105I GACNNNNNGTC 1 cut(s) 2515
EarI CTCTTC 3 cut(s) 1398, 1974, 2035
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 3 cut(s) 833, 1439, 2007
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 4 cut(s) 1286, 1677, 1719, 2007
Eco31I GGTCTC 1 cut(s) 2592
Eco47I GGWCC 4 cut(s) 4, 739, 1089, 2350
Eco57I CTGAAG 4 cut(s) 383, 509, 701, 2010
Eco72I CACGTG 1 cut(s) 946
Eco81I CCTNAGG 2 cut(s) 807, 2370
EcoO109I RGGNCCY 4 cut(s) 1673, 1674, 1715, 1716
EcoRII CCWGG 5 cut(s) 213, 1294, 1311, 1609, 2593
EcoT14I CCWWGG 3 cut(s) 833, 1439, 2007
EcoT22I ATGCAT 3 cut(s) 57, 346, 1606
EcoT38I GRGCYC 4 cut(s) 1286, 1677, 1719, 2007
ErhI CCWWGG 3 cut(s) 833, 1439, 2007
Esp3I CGTCTC 1 cut(s) 319
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 2072, 2104
FaqI GGGAC 5 cut(s) 113, 752, 1216, 2205, 2713
FauI CCCGC 2 cut(s) 990, 2220
FauNDI CATATG 1 cut(s) 283
FokI GGATG 7 cut(s) 56, 80, 230, 850, 1138, 2024, 2786
FriOI GRGCYC 4 cut(s) 1286, 1677, 1719, 2007
FspBI CTAG 5 cut(s) 180, 210, 834, 1371, 1854
GsaI CCCAGC 2 cut(s) 1803, 2700
GsuI CTGGAG 1 cut(s) 413
HaeIII GGCC 8 cut(s) 77, 89, 971, 1473, 1675, 1717, 1842, 2005
HapII CCGG 2 cut(s) 2353, 2862
HgaI GACGC 1 cut(s) 1756
HincII GTYRAC 3 cut(s) 468, 542, 2389
HindII GTYRAC 3 cut(s) 468, 542, 2389
HindIII AAGCTT 2 cut(s) 521, 2357
HpaII CCGG 2 cut(s) 2353, 2862
HphI GGTGA 4 cut(s) 1599, 1866, 2405, 2808
Hpy166II GTNNAC 8 cut(s) 468, 542, 590, 677, 870, 943, 2389, 2437
Hpy8I GTNNAC 8 cut(s) 468, 542, 590, 677, 870, 943, 2389, 2437
Hpy99I CGWCG 2 cut(s) 13, 1750
HpyAV CCTTC 8 cut(s) 101, 921, 1704, 1893, 2074, 2405, 2555, 2638
HpyCH4III ACNGT 8 cut(s) 392, 798, 940, 2018, 2169, 2217, 2393, 2704
HpyCH4IV ACGT 5 cut(s) 307, 945, 1009, 2155, 2557
HpySE526I ACGT 5 cut(s) 307, 945, 1009, 2155, 2557
Kpn2I TCCGGA 1 cut(s) 2352
Kzo9I GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
LmnI GCTCC 8 cut(s) 475, 559, 1064, 1084, 1683, 1987, 2089, 2686
MaeI CTAG 5 cut(s) 180, 210, 834, 1371, 1854
MaeII ACGT 5 cut(s) 307, 945, 1009, 2155, 2557
MaeIII GTNAC 5 cut(s) 609, 1511, 1948, 2163, 2814
MalI GATC 7 cut(s) 396, 417, 876, 1171, 1390, 1758, 1923
MbiI CCGCTC 1 cut(s) 1087
MboI GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
MflI RGATCY 2 cut(s) 1169, 1388
MhlI GDGCHC 7 cut(s) 945, 1286, 1338, 1677, 1719, 2007, 2739
MlsI TGGCCA 1 cut(s) 1842
MluNI TGGCCA 1 cut(s) 1842
MlyI GAGTC 4 cut(s) 137, 1238, 1637, 2849
MmeI TCCRAC 2 cut(s) 739, 2479
Mox20I TGGCCA 1 cut(s) 1842
Mph1103I ATGCAT 3 cut(s) 57, 346, 1606
MroI TCCGGA 1 cut(s) 2352
MscI TGGCCA 1 cut(s) 1842
MslI CAYNNNNRTG 2 cut(s) 575, 1331
Msp20I TGGCCA 1 cut(s) 1842
MspA1I CMGCKG 6 cut(s) 130, 1955, 1993, 2227, 2630, 2696
MspCI CTTAAG 1 cut(s) 1765
MspI CCGG 2 cut(s) 2353, 2862
MspR9I CCNGG 5 cut(s) 215, 1296, 1313, 1611, 2595
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 5 cut(s) 215, 1296, 1313, 1611, 2595
NdeI CATATG 1 cut(s) 283
NdeII GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
NheI GCTAGC 1 cut(s) 209
NlaIV GGNNCC 9 cut(s) 6, 740, 1506, 1675, 1692, 1717, 2005, 2085, 2799
NmuCI GTSAC 4 cut(s) 1511, 1948, 2163, 2814
NsiI ATGCAT 3 cut(s) 57, 346, 1606
NspI RCATGY 1 cut(s) 2515
PagI TCATGA 1 cut(s) 1326
PaqCI CACCTGC 1 cut(s) 845
PasI CCCWGGG 1 cut(s) 1312
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 2511
PcsI WCGNNNNNNNCGW 1 cut(s) 2554
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 6 cut(s) 137, 506, 580, 956, 1019, 1723
PleI GAGTC 4 cut(s) 137, 1238, 1636, 2849
PmaCI CACGTG 1 cut(s) 946
PmlI CACGTG 1 cut(s) 946
PpsI GAGTC 4 cut(s) 137, 1238, 1636, 2849
Ppu21I YACGTR 1 cut(s) 946
PscI ACATGT 1 cut(s) 2511
PshAI GACNNNNGTC 1 cut(s) 1208
PshBI ATTAAT 1 cut(s) 1806
Psp1406I AACGTT 2 cut(s) 307, 2155
Psp6I CCWGG 5 cut(s) 213, 1294, 1311, 1609, 2593
PspCI CACGTG 1 cut(s) 946
PspFI CCCAGC 2 cut(s) 1799, 2696
PspGI CCWGG 5 cut(s) 213, 1294, 1311, 1609, 2593
PspN4I GGNNCC 9 cut(s) 6, 740, 1506, 1675, 1692, 1717, 2005, 2085, 2799
PspOMI GGGCCC 3 cut(s) 1673, 1715, 2003
PstI CTGCAG 1 cut(s) 2695
PstNI CAGNNNCTG 5 cut(s) 146, 1067, 1135, 2630, 2684
PsuI RGATCY 2 cut(s) 1169, 1388
PvuII CAGCTG 4 cut(s) 1955, 1993, 2630, 2696
RsaI GTAC 5 cut(s) 226, 597, 762, 771, 1110
RsaNI GTAC 5 cut(s) 225, 596, 761, 770, 1109
RseI CAYNNNNRTG 2 cut(s) 575, 1331
Sau3AI GATC 7 cut(s) 394, 415, 874, 1169, 1388, 1756, 1921
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 4 cut(s) 137, 1238, 1637, 2849
ScrFI CCNGG 5 cut(s) 215, 1296, 1313, 1611, 2595
SduI GDGCHC 7 cut(s) 945, 1286, 1338, 1677, 1719, 2007, 2739
SfcI CTRYAG 1 cut(s) 2691
SinI GGWCC 4 cut(s) 4, 739, 1089, 2350
SmiMI CAYNNNNRTG 2 cut(s) 575, 1331
SmlI CTYRAG 3 cut(s) 159, 749, 1765
SmoI CTYRAG 3 cut(s) 159, 749, 1765
SseBI AGGCCT 1 cut(s) 77
SsiI CCGC 9 cut(s) 65, 86, 130, 149, 194, 997, 1087, 1880, 2227
SspI AATATT 1 cut(s) 256
SspMI CTAG 5 cut(s) 180, 210, 834, 1371, 1854
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 5 cut(s) 213, 1294, 1311, 1609, 2593
StyI CCWWGG 3 cut(s) 833, 1439, 2007
TaaI ACNGT 8 cut(s) 392, 798, 940, 2018, 2169, 2217, 2393, 2704
TaiI ACGT 5 cut(s) 310, 948, 1012, 2158, 2560
TaqI TCGA 4 cut(s) 11, 1415, 2020, 2038
TatI WGTACW 4 cut(s) 224, 760, 769, 1108
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 6 cut(s) 137, 506, 580, 956, 1019, 1723
TscAI CASTG 6 cut(s) 397, 468, 877, 945, 1516, 2172
TseFI GTSAC 4 cut(s) 1511, 1948, 2163, 2814
Tsp45I GTSAC 4 cut(s) 1511, 1948, 2163, 2814
TspGWI ACGGA 2 cut(s) 301, 572
TspRI CASTG 6 cut(s) 397, 468, 877, 945, 1516, 2172
Vha464I CTTAAG 1 cut(s) 1765
VneI GTGCAC 1 cut(s) 941
VpaK11BI GGWCC 4 cut(s) 4, 739, 1089, 2350
VspI ATTAAT 1 cut(s) 1806
XapI RAATTY 6 cut(s) 237, 1260, 1354, 1614, 2286, 2711
XceI RCATGY 1 cut(s) 2515
XmaJI CCTAGG 1 cut(s) 833
XspI CTAG 5 cut(s) 180, 210, 834, 1371, 1854
ZrmI AGTACT 1 cut(s) 226
Zsp2I ATGCAT 3 cut(s) 57, 346, 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.