Prupe.6G138300_v2.0.a1

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001817
Physical Location & Seq
Forward (+)
11237087 .. 11246485
9399 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G138300.3

Sequence Viewer

Length: 2853 bp
ATGAGGTTCTCTCTGTCTCTATCTCTGTCTCTGTCTCTGTCTCTCTCTGATGTATCTCAAGCTAATATTACATTCTCATTCTTAATAAAATGTCCGAAGGATGAAACCATGCATTCCGGGGCGGATTTGTTAGCCTTCCAGGCTGCCCTGAATAAGGACATAGAAGGAGATGTGTCTGCTTCCCAGCCGTCTGATTCAGACACAGCGGTTTTGTGTCAAGGAAGCAATAATACTTCTAGTCAGTCATTACTGCAGTTGAGTATTGCTAGCCAGGATGAAAATACAGCTGGTCAAATTCAACAAGACCAAAAAATTGCACAACATCGAGAACTGCATTTGTATGAAATGGAACCAAAGCAACAGGGATCCATTGCTGAGAATATGCAACAGAAAAATGATGCCTCAAAGGTACTCAATCACTTTCCTTTACCCCAGAAGCAACCACATGGTGATCTTCAAGAAGGACAGGCAGAACAGAAGCCACTCCAGATTCCTGAAACAACTGGAATGCTGATTTCTGAAAAACACCCAAGCTCAACAGAGGAGCACGGCATAGCACCTAATCTCAAAAGCGAATCTCAGTACTTAAAGCTTCAGAAGATGAGCAGTCAACAATCAATGATCACAGAGCAACCAAGAAACCCTTCGAATCAAAGCAGACAAGTAGTATTTTACCAGTTATATCCCCTCTTATTGCCCCAACTAAATAAAGACAGAGGAAGGCAACTTACTACTCTATTTGGTAGACTCAAGAGCCGTCAAATCCTCAAAGATGCTTTTGTCCGGGAACTTAAAAGTATTGTTGGGGATGAGATGCTCAGAATGGCAGTAAAGGAAGCGCCCCTAATGCCTAGTCCTAACCATCGGTTACTGCCTAAGGCCTCGGTCCCGCAACAACCTCCAAGGATGCTATCTATTAGTGCAGGTGCTGCACAGTTGACTAACCCTCGTTCATCTGCACTTCATCTGAGAGGTCCCAATTCTGCCACTGACCCATCACACAGTCTCCCTTCAACAGTTCAGGTACAGACTGATTCAAGCCATCCGCTCATTAAAAATAGTGCTAGAAAATTGCCAGAAGCAGAATGTCACTCAGATTCTGAGGGAATGCAAGTAAGTCAAATTTCTTCCTCTAGTGTGGTAGCTACCAATCAAGAAAGGGAGTGCTTCTCAGTTCCTATGCATGGAAACGCTGGTGGTACTTGTCACCTATTTTCAGGGACAAGTGTCAATACTTGCGCATTGCCTGTCAAACCGCAGCCTTCTGATGCACAACTGAGACAAGTCCTACAACATCAAAGCATAGGTTCTACTCAGTCAGGTGGGGAAGCACGTGGAGCGAACATCATGAGTGTGTCCAAAACGGAGAGGCAAAATTCTATGAATGAACCTAGTAGACTGCAAGGTGGATCTCTTACTCACTTTACAACTAATGCAACTTTGCAACAGAATCCTAATCCTTCACAATCCTCAAATAAAGAGCAGAGTTCCCGTCCTGTGTCATCAATGGCTTATGTCAAACAGGAATTGATTGATCAGACTGCTGAGCAGCAGCACAAGCCTCCATTGCCTAGTTCACATGGATTGCCTTCGGTTTCTGCTGGAGTACTTGAACAGGGAAATGCATCATCTGTAATTTCAATGGATGAGTCCTTAGAGAAGCAGCCTTCTAGAATGGGTGTTTCATCATCGCCTTCGTCGTCTACAATCACAGTGCCTCAAAGTTCGGTGTCTCCTTCCTTCATGATGCAAGTCAATCCTAGTGTCTCGTTAGGCCCTCGGATAAGCTCTGGAACCTCTCCTTGTGGGATTAATAATAAAACACCTCCAAAAAAGCCTTCTATTGGCCGAAAGAAGCCGCTTGAAGCACTTGGTTCTTCACCACCGCAATCAAGTAAGAAGCAAAAAGTAAGTGGGGCGTTTTCGGATATTGAACAACTCAATGATGTCACTGCCGTCAGTGGAGTTAATCTTAGGGAAGAGGAAGAGCAGCTGTTTCCGGGGCCCAAGGAGGATAGTCGAGCTTCAGAAGCCTCTCGAAAATCTGTGCAAAAAGAAGAGGAAAGGCTGATTTTGGAGAAGGCTCCACTGCAGAAAAAATTAGCTGAAATTATGGTCAAATGTGGTCTGAAAAGTGTAACCCATGACGTGGAGCGATGCTTGTCACTGTGTGTGGAGGAAAGAATGAGGGGACTAATAAATAATTTGATCCGACTGTCAAAGCAGCGGGTTGATGCTGAGAAATCAAGACACCACACTATTACTAACTCTGATGTTCGGCAACAAATTATGGATCTCAATCAGAAAGCTAGGGAAGAATGGGAGAAAAAGCAGGCTGGGGCAGAAAAGATCCGGAGGCTTAATGAGCCTGAGGTTGACAATGGAGTCGATGGAGACGAGGAGAAGGATGAAAGCCATCCTAAATCATTTAAGACAAACATAGAGGCAGATGGCAAACTGAGGACAACAGCAGCAAATGTTGCTGCCCGTGCTGCAGTTGGGGGAGACGACATGTTCTCAAAATGGCAACTCATGGCACAAGCCCGGCAAAAACATGAAGGTGAGATTGATGCGGCGTCTGGGTCCCAGCCAGGTAAAGATGTAAACCACAAACCTACATCAACAACTGGAAGGATCATGAAGGGCAGTCAAGAAGCAGAGAAAAGGGGTGGTCCGGCTCCTGTCGCTAGAGCTGGGCCAATTAGAAAATCTGGAAGGAACCAAGTTATCATGCCTCAAACAAGGATGGCTCGTACTATATCTGTCAAGGATATGATTGCTGCCTTGGAAAGGGAACCTCAGATGTCGAAGTCTATGTTGATATATCGGTTGCATGAGAGAATCCAATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

951

Amino Acids

103.8

Weight (kDa)

8.87

Isoelectric Point (pI)

59.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 914
AasI GACNNNNNNGTC 1 cut(s) 2384
Acc16I TGCGCA 1 cut(s) 1240
Acc36I ACCTGC 1 cut(s) 914
AccB7I CCANNNNNTGG 1 cut(s) 2149
AccBSI CCGCTC 1 cut(s) 1048
AccI GTMKAC 3 cut(s) 745, 1396, 1703
AccIII TCCGGA 1 cut(s) 2352
AciI CCGC 9 cut(s) 122, 206, 890, 1046, 1256, 1859, 1886, 2227, 2573
AclWI GGATC 7 cut(s) 360, 373, 1417, 2203, 2301, 2344, 2642
AcoI YGGCCR 1 cut(s) 1846
AcsI RAATTY 3 cut(s) 294, 1122, 1375
AcuI CTGAAG 2 cut(s) 578, 2010
AcvI CACGTG 1 cut(s) 1334
AcyI GRCGYC 1 cut(s) 2576
AdeI CACNNNGTG 2 cut(s) 449, 2171
AfaI GTAC 6 cut(s) 411, 584, 1026, 1201, 1608, 2755
AfiI CCNNNNNNNGG 5 cut(s) 154, 1184, 1844, 2149, 2790
AflIII ACRYGT 1 cut(s) 2511
AgsI TTSAA 8 cut(s) 299, 458, 1014, 1038, 1613, 1641, 1865, 1934
AjiI CACGTC 1 cut(s) 2149
AjnI CCWGG 3 cut(s) 138, 270, 2590
AjuI GAANNNNNNNTTGG 4 cut(s) 628, 660, 1822, 1854
Alw21I GWGCWC 1 cut(s) 549
AlwI GGATC 7 cut(s) 360, 373, 1417, 2203, 2301, 2344, 2642
AlwNI CAGNNNCTG 2 cut(s) 929, 1100
Aor13HI TCCGGA 1 cut(s) 2352
AoxI GGCC 5 cut(s) 879, 1774, 1846, 2003, 2696
ApaI GGGCCC 1 cut(s) 2007
ApoI RAATTY 3 cut(s) 294, 1122, 1375
AseI ATTAAT 1 cut(s) 1812
Asp700I GAANNNNTTC 1 cut(s) 643
AspLEI GCGC 2 cut(s) 841, 1241
AspS9I GGNCC 8 cut(s) 886, 974, 1775, 2003, 2004, 2583, 2672, 2696
AsuC2I CCSGG 4 cut(s) 118, 785, 2001, 2545
AsuHPI GGTGA 4 cut(s) 461, 1199, 1872, 2573
AsuII TTCGAA 1 cut(s) 647
AsuNHI GCTAGC 1 cut(s) 266
AvaII GGWCC 4 cut(s) 886, 974, 2583, 2672
AxyI CCTNAGG 2 cut(s) 876, 2370
BaeGI GKGCMC 1 cut(s) 2007
BamHI GGATCC 1 cut(s) 365
BanII GRGCYC 1 cut(s) 2007
BarI GAAGNNNNNNTAC 4 cut(s) 214, 246, 712, 744
BbrPI CACGTG 1 cut(s) 1334
Bbv12I GWGCWC 1 cut(s) 549
BccI CCATC 7 cut(s) 870, 1003, 1050, 2384, 2424, 2444, 2740
BceAI ACGGC 4 cut(s) 172, 565, 741, 1940
BciT130I CCWGG 3 cut(s) 140, 272, 2592
BclI TGATCA 2 cut(s) 621, 1534
BcnI CCSGG 4 cut(s) 118, 785, 2001, 2545
BfmI CTRYAG 3 cut(s) 251, 2090, 2493
BfoI RGCGCY 1 cut(s) 842
BfuAI ACCTGC 1 cut(s) 914
BglI GCCNNNNNGGC 1 cut(s) 140
BlpI GCTNAGC 1 cut(s) 1545
BmcAI AGTACT 2 cut(s) 584, 1608
Bme1390I CCNGG 7 cut(s) 118, 140, 272, 785, 2001, 2545, 2592
Bme18I GGWCC 4 cut(s) 886, 974, 2583, 2672
BmgBI CACGTC 1 cut(s) 2149
BmgT120I GGNCC 8 cut(s) 886, 974, 1775, 2003, 2004, 2583, 2672, 2696
BmrFI CCNGG 7 cut(s) 118, 140, 272, 785, 2001, 2545, 2592
BmtI GCTAGC 1 cut(s) 270
BoxI GACNNNNGTC 1 cut(s) 1226
BplI GAGNNNNNCTC 3 cut(s) 27, 1154, 1186
BpmI CTGGAG 2 cut(s) 470, 1623
Bpu1102I GCTNAGC 1 cut(s) 1545
Bpu14I TTCGAA 1 cut(s) 647
BpuEI CTTGAG 2 cut(s) 42, 734
BpuMI CCSGG 4 cut(s) 118, 785, 2001, 2545
BsaAI YACGTR 1 cut(s) 1334
BsaBI GATNNNNATC 1 cut(s) 2298
BsaHI GRCGYC 1 cut(s) 2576
BsaJI CCNNGG 7 cut(s) 117, 882, 902, 1778, 2000, 2007, 2784
BsaWI WCCGGW 1 cut(s) 2352
BsaXI ACNNNNNCTCC 2 cut(s) 990, 1020
Bsc4I CCNNNNNNNGG 5 cut(s) 154, 1184, 1844, 2149, 2790
Bse1I ACTGG 3 cut(s) 508, 676, 2632
Bse21I CCTNAGG 2 cut(s) 876, 2370
Bse3DI GCAATG 3 cut(s) 369, 1241, 1565
Bse8I GATNNNNATC 1 cut(s) 2298
BseAI TCCGGA 1 cut(s) 2352
BseBI CCWGG 3 cut(s) 140, 272, 2592
BseDI CCNNGG 7 cut(s) 117, 882, 902, 1778, 2000, 2007, 2784
BseGI GGATG 9 cut(s) 106, 280, 814, 912, 1042, 1651, 2413, 2416, 2751
BseJI GATNNNNATC 1 cut(s) 2298
BseLI CCNNNNNNNGG 5 cut(s) 154, 1184, 1844, 2149, 2790
BseMI GCAATG 3 cut(s) 369, 1241, 1565
BseNI ACTGG 3 cut(s) 508, 676, 2632
BseRI GAGGAG 2 cut(s) 557, 2414
BseSI GKGCMC 1 cut(s) 2007
BseYI CCCAGC 4 cut(s) 183, 2336, 2586, 2693
BsgI GTGCAG 3 cut(s) 915, 942, 942
BshFI GGCC 5 cut(s) 881, 1776, 1848, 2005, 2698
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 6 cut(s) 117, 784, 2000, 2353, 2545, 2675
BslFI GGGAC 5 cut(s) 872, 960, 1234, 2205, 2569
BslI CCNNNNNNNGG 5 cut(s) 154, 1184, 1844, 2149, 2790
BsmBI CGTCTC 2 cut(s) 2388, 2499
BsmFI GGGAC 5 cut(s) 872, 960, 1234, 2205, 2569
BsmI GAATGC 3 cut(s) 112, 513, 1113
BsnI GGCC 5 cut(s) 881, 1776, 1848, 2005, 2698
Bsp119I TTCGAA 1 cut(s) 647
Bsp120I GGGCCC 1 cut(s) 2003
Bsp1286I GDGCHC 2 cut(s) 549, 2007
Bsp13I TCCGGA 1 cut(s) 2352
Bsp143I GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
Bsp1720I GCTNAGC 1 cut(s) 1545
BspACI CCGC 9 cut(s) 122, 206, 890, 1046, 1256, 1859, 1886, 2227, 2573
BspANI GGCC 5 cut(s) 881, 1776, 1848, 2005, 2698
BspEI TCCGGA 1 cut(s) 2352
BspHI TCATGA 3 cut(s) 1347, 1743, 2637
BspMAI CTGCAG 3 cut(s) 255, 2094, 2497
BspMI ACCTGC 1 cut(s) 914
BspOI GCTAGC 1 cut(s) 270
BspPI GGATC 7 cut(s) 360, 373, 1417, 2203, 2301, 2344, 2642
BspQI GCTCTTC 1 cut(s) 1980
BspT104I TTCGAA 1 cut(s) 647
BsrBI CCGCTC 1 cut(s) 1048
BsrDI GCAATG 3 cut(s) 369, 1241, 1565
BsrI ACTGG 3 cut(s) 508, 676, 2632
BssECI CCNNGG 7 cut(s) 117, 882, 902, 1778, 2000, 2007, 2784
BssMI GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
BssNI GRCGYC 1 cut(s) 2576
BssT1I CCWWGG 3 cut(s) 902, 2007, 2784
Bst2UI CCWGG 3 cut(s) 140, 272, 2592
Bst4CI ACNGT 6 cut(s) 936, 1004, 1018, 1714, 2169, 2217
Bst6I CTCTTC 3 cut(s) 1974, 1980, 2052
BstACI GRCGYC 1 cut(s) 2576
BstAPI GCANNNNNTGC 2 cut(s) 929, 2480
BstBAI YACGTR 1 cut(s) 1334
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 2 cut(s) 268, 2334
BstENI CCTNNNNNAGG 2 cut(s) 152, 2788
BstF5I GGATG 9 cut(s) 106, 280, 814, 912, 1042, 1651, 2413, 2416, 2751
BstH2I RGCGCY 1 cut(s) 842
BstHHI GCGC 2 cut(s) 841, 1241
BstKTI GATC 9 cut(s) 368, 454, 624, 1412, 1537, 2211, 2296, 2352, 2637
BstMBI GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
BstNI CCWGG 3 cut(s) 140, 272, 2592
BstNSI RCATGY 1 cut(s) 2515
BstPAI GACNNNNGTC 1 cut(s) 1226
BstSCI CCNGG 7 cut(s) 116, 138, 270, 783, 1999, 2543, 2590
BstSFI CTRYAG 3 cut(s) 251, 2090, 2493
BstSLI GKGCMC 1 cut(s) 2007
BstX2I RGATCY 4 cut(s) 365, 1409, 2293, 2349
BstYI RGATCY 4 cut(s) 365, 1409, 2293, 2349
Bsu36I CCTNAGG 2 cut(s) 876, 2370
BsuRI GGCC 5 cut(s) 881, 1776, 1848, 2005, 2698
BtgZI GCGATG 2 cut(s) 1674, 2170
BtrI CACGTC 1 cut(s) 2149
BtsCI GGATG 9 cut(s) 106, 280, 814, 912, 1042, 1651, 2413, 2416, 2751
BtsI GCAGTG 2 cut(s) 1950, 2087
BtsIMutI CAGTG 6 cut(s) 987, 1719, 1950, 1966, 2087, 2165
BveI ACCTGC 1 cut(s) 914
Cac8I GCNNGC 2 cut(s) 268, 2334
CaiI CAGNNNCTG 2 cut(s) 929, 1100
CciI TCATGA 3 cut(s) 1347, 1743, 2637
CfoI GCGC 2 cut(s) 841, 1241
Cfr13I GGNCC 8 cut(s) 886, 974, 1775, 2003, 2004, 2583, 2672, 2696
CseI GACGC 1 cut(s) 2565
Csp6I GTAC 6 cut(s) 410, 583, 1025, 1200, 1607, 2754
CviQI GTAC 6 cut(s) 410, 583, 1025, 1200, 1607, 2754
DpnI GATC 9 cut(s) 367, 453, 623, 1411, 1536, 2210, 2295, 2351, 2636
DpnII GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
DraIII CACNNNGTG 2 cut(s) 449, 2171
DrdI GACNNNNNNGTC 1 cut(s) 2384
DseDI GACNNNNNNGTC 1 cut(s) 2384
EaeI YGGCCR 1 cut(s) 1846
Eam1104I CTCTTC 3 cut(s) 1974, 1980, 2052
EarI CTCTTC 3 cut(s) 1974, 1980, 2052
EciI GGCGGA 1 cut(s) 137
Eco130I CCWWGG 3 cut(s) 902, 2007, 2784
Eco147I AGGCCT 1 cut(s) 881
Eco24I GRGCYC 1 cut(s) 2007
Eco47I GGWCC 4 cut(s) 886, 974, 2583, 2672
Eco57I CTGAAG 2 cut(s) 578, 2010
Eco72I CACGTG 1 cut(s) 1334
Eco81I CCTNAGG 2 cut(s) 876, 2370
EcoNI CCTNNNNNAGG 2 cut(s) 152, 2788
EcoO109I RGGNCCY 4 cut(s) 974, 1775, 2003, 2583
EcoRII CCWGG 3 cut(s) 138, 270, 2590
EcoT14I CCWWGG 3 cut(s) 902, 2007, 2784
EcoT22I ATGCAT 3 cut(s) 114, 1185, 1627
EcoT38I GRGCYC 1 cut(s) 2007
ErhI CCWWGG 3 cut(s) 902, 2007, 2784
Esp3I CGTCTC 2 cut(s) 2388, 2499
FalI AAGNNNNNCTT 2 cut(s) 628, 660
FaqI GGGAC 5 cut(s) 872, 960, 1234, 2205, 2569
FauI CCCGC 2 cut(s) 897, 2220
FbaI TGATCA 2 cut(s) 621, 1534
FblI GTMKAC 3 cut(s) 745, 1396, 1703
FokI GGATG 9 cut(s) 113, 287, 821, 919, 1029, 1658, 2403, 2420, 2758
FriOI GRGCYC 1 cut(s) 2007
FspI TGCGCA 1 cut(s) 1240
GlaI GCGC 2 cut(s) 840, 1240
GsaI CCCAGC 4 cut(s) 187, 2340, 2590, 2697
GsuI CTGGAG 2 cut(s) 470, 1623
HaeII RGCGCY 1 cut(s) 842
HaeIII GGCC 5 cut(s) 881, 1776, 1848, 2005, 2698
HapII CCGG 6 cut(s) 117, 784, 2000, 2353, 2545, 2675
HgaI GACGC 1 cut(s) 2565
HhaI GCGC 2 cut(s) 841, 1241
Hin1I GRCGYC 1 cut(s) 2576
Hin6I GCGC 2 cut(s) 839, 1239
HinP1I GCGC 2 cut(s) 839, 1239
HincII GTYRAC 3 cut(s) 611, 939, 2377
HindII GTYRAC 3 cut(s) 611, 939, 2377
HindIII AAGCTT 1 cut(s) 590
HpaII CCGG 6 cut(s) 117, 784, 2000, 2353, 2545, 2675
HphI GGTGA 4 cut(s) 461, 1199, 1872, 2573
Hpy166II GTNNAC 8 cut(s) 611, 746, 939, 1397, 1577, 1704, 2377, 2605
Hpy8I GTNNAC 8 cut(s) 611, 746, 939, 1397, 1577, 1704, 2377, 2605
Hpy99I CGWCG 1 cut(s) 1702
HpyCH4III ACNGT 6 cut(s) 936, 1004, 1018, 1714, 2169, 2217
HpyCH4IV ACGT 2 cut(s) 1333, 2148
HpySE526I ACGT 2 cut(s) 1333, 2148
Hsp92I GRCGYC 1 cut(s) 2576
HspAI GCGC 2 cut(s) 839, 1239
KflI GGGWCCC 1 cut(s) 2583
Kpn2I TCCGGA 1 cut(s) 2352
Ksp22I TGATCA 2 cut(s) 621, 1534
Kzo9I GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
LguI GCTCTTC 1 cut(s) 1980
LmnI GCTCC 5 cut(s) 544, 1337, 2089, 2152, 2683
MaeII ACGT 2 cut(s) 1333, 2148
MaeIII GTNAC 6 cut(s) 867, 1088, 1205, 1948, 2137, 2163
MalI GATC 9 cut(s) 367, 453, 623, 1411, 1536, 2210, 2295, 2351, 2636
MbiI CCGCTC 1 cut(s) 1048
MboI GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
MboII GAAGA 8 cut(s) 446, 610, 1119, 1869, 1991, 1997, 2069, 2327
MflI RGATCY 4 cut(s) 365, 1409, 2293, 2349
MhlI GDGCHC 2 cut(s) 549, 2007
MlyI GAGTC 3 cut(s) 741, 1658, 2394
MmeI TCCRAC 1 cut(s) 2236
Mph1103I ATGCAT 3 cut(s) 114, 1185, 1627
MroI TCCGGA 1 cut(s) 2352
MroXI GAANNNNTTC 1 cut(s) 643
MseI TTAA 8 cut(s) 83, 587, 792, 1053, 1812, 1968, 2361, 2430
MslI CAYNNNNRTG 3 cut(s) 339, 1352, 2559
MspA1I CMGCKG 4 cut(s) 206, 287, 1993, 2227
MspI CCGG 6 cut(s) 117, 784, 2000, 2353, 2545, 2675
MspR9I CCNGG 7 cut(s) 118, 140, 272, 785, 2001, 2545, 2592
Mva1269I GAATGC 3 cut(s) 112, 513, 1113
MvaI CCWGG 3 cut(s) 140, 272, 2592
NciI CCSGG 4 cut(s) 118, 785, 2001, 2545
NdeII GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
NheI GCTAGC 1 cut(s) 266
NmuCI GTSAC 4 cut(s) 1088, 1205, 1948, 2163
NsbI TGCGCA 1 cut(s) 1240
NsiI ATGCAT 3 cut(s) 114, 1185, 1627
NspI RCATGY 1 cut(s) 2515
NspV TTCGAA 1 cut(s) 647
PagI TCATGA 3 cut(s) 1347, 1743, 2637
PaqCI CACCTGC 1 cut(s) 914
PceI AGGCCT 1 cut(s) 881
PciI ACATGT 1 cut(s) 2511
PciSI GCTCTTC 1 cut(s) 1980
PcsI WCGNNNNNNNCGW 2 cut(s) 1697, 2394
PctI GAATGC 3 cut(s) 112, 513, 1113
PdmI GAANNNNTTC 1 cut(s) 643
PfeI GAWTC 8 cut(s) 194, 490, 575, 649, 1034, 1097, 1450, 2841
PflMI CCANNNNNTGG 1 cut(s) 2149
PfoI TCCNGGA 1 cut(s) 783
PleI GAGTC 3 cut(s) 741, 1657, 2393
PmaCI CACGTG 1 cut(s) 1334
PmlI CACGTG 1 cut(s) 1334
PpsI GAGTC 3 cut(s) 741, 1657, 2393
Ppu21I YACGTR 1 cut(s) 1334
PpuMI RGGWCCY 2 cut(s) 974, 2583
PscI ACATGT 1 cut(s) 2511
PshAI GACNNNNGTC 1 cut(s) 1226
PshBI ATTAAT 1 cut(s) 1812
Psp5II RGGWCCY 2 cut(s) 974, 2583
Psp6I CCWGG 3 cut(s) 138, 270, 2590
PspCI CACGTG 1 cut(s) 1334
PspFI CCCAGC 4 cut(s) 183, 2336, 2586, 2693
PspGI CCWGG 3 cut(s) 138, 270, 2590
PspOMI GGGCCC 1 cut(s) 2003
PspPI GGNCC 8 cut(s) 886, 974, 1775, 2003, 2004, 2583, 2672, 2696
PspPPI RGGWCCY 2 cut(s) 974, 2583
PstI CTGCAG 3 cut(s) 255, 2094, 2497
PstNI CAGNNNCTG 2 cut(s) 929, 1100
PsuI RGATCY 4 cut(s) 365, 1409, 2293, 2349
PvuII CAGCTG 2 cut(s) 287, 1993
RsaI GTAC 6 cut(s) 411, 584, 1026, 1201, 1608, 2755
RsaNI GTAC 6 cut(s) 410, 583, 1025, 1200, 1607, 2754
RseI CAYNNNNRTG 3 cut(s) 339, 1352, 2559
SapI GCTCTTC 1 cut(s) 1980
SaqAI TTAA 8 cut(s) 83, 587, 792, 1053, 1812, 1968, 2361, 2430
Sau3AI GATC 9 cut(s) 365, 451, 621, 1409, 1534, 2208, 2293, 2349, 2634
Sau96I GGNCC 8 cut(s) 886, 974, 1775, 2003, 2004, 2583, 2672, 2696
ScaI AGTACT 2 cut(s) 584, 1608
SchI GAGTC 3 cut(s) 741, 1658, 2394
ScrFI CCNGG 7 cut(s) 118, 140, 272, 785, 2001, 2545, 2592
SduI GDGCHC 2 cut(s) 549, 2007
SfcI CTRYAG 3 cut(s) 251, 2090, 2493
SfuI TTCGAA 1 cut(s) 647
SinI GGWCC 4 cut(s) 886, 974, 2583, 2672
SmiMI CAYNNNNRTG 3 cut(s) 339, 1352, 2559
SmlI CTYRAG 2 cut(s) 57, 749
SmoI CTYRAG 2 cut(s) 57, 749
SseBI AGGCCT 1 cut(s) 881
SsiI CCGC 9 cut(s) 122, 206, 890, 1046, 1256, 1859, 1886, 2227, 2573
SspI AATATT 1 cut(s) 67
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 7 cut(s) 116, 138, 270, 783, 1999, 2543, 2590
StyI CCWWGG 3 cut(s) 902, 2007, 2784
TaaI ACNGT 6 cut(s) 936, 1004, 1018, 1714, 2169, 2217
TaiI ACGT 2 cut(s) 1336, 2151
TaqI TCGA 6 cut(s) 325, 647, 2020, 2038, 2388, 2807
TaqII GACCGA 1 cut(s) 874
TatI WGTACW 2 cut(s) 582, 1606
TauI GCSGC 2 cut(s) 1861, 2576
TfiI GAWTC 8 cut(s) 194, 490, 575, 649, 1034, 1097, 1450, 2841
Tru1I TTAA 8 cut(s) 83, 587, 792, 1053, 1812, 1968, 2361, 2430
Tru9I TTAA 8 cut(s) 83, 587, 792, 1053, 1812, 1968, 2361, 2430
TscAI CASTG 6 cut(s) 994, 1719, 1957, 1966, 2094, 2172
TseFI GTSAC 4 cut(s) 1088, 1205, 1948, 2163
Tsp45I GTSAC 4 cut(s) 1088, 1205, 1948, 2163
TspGWI ACGGA 1 cut(s) 1379
TspRI CASTG 6 cut(s) 994, 1719, 1957, 1966, 2094, 2172
Van91I CCANNNNNTGG 1 cut(s) 2149
VpaK11BI GGWCC 4 cut(s) 886, 974, 2583, 2672
VspI ATTAAT 1 cut(s) 1812
XagI CCTNNNNNAGG 2 cut(s) 152, 2788
XapI RAATTY 3 cut(s) 294, 1122, 1375
XbaI TCTAGA 1 cut(s) 1670
XceI RCATGY 1 cut(s) 2515
XmiI GTMKAC 3 cut(s) 745, 1396, 1703
XmnI GAANNNNTTC 1 cut(s) 643
ZrmI AGTACT 2 cut(s) 584, 1608
Zsp2I ATGCAT 3 cut(s) 114, 1185, 1627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.