Rroxscaffold_3G00243360

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001817
Physical Location & Seq
Reverse (-)
35883404 .. 35891715
8312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243360.1

Sequence Viewer

Length: 2937 bp
ATGGACCCGTCGATTATGAAGAAGCTCTTGGAAGACGATGAGGATGAAACAATGCATTCGGGGGCGGATGTGGAGGCCTTTCAGGCGGCCCTGAATAGGGACATTGAAGGTGATGTGTCAGCTTCTCAGCGGTCTGATTCAGACTCTGCAGTTTTGTCTCAAGGAAGCAATAATACTTCTAGTCAATCATTACCACAGTTGCATACTGCTAGCCAGGATGAAAGTACTGCTGGTCAAATTCAGCATGACCAAAATATTGCACAGCGGCGAGAAATGCAGTCATATGAAATGGAGTTAAAGCAACAACGTTCCATCTCTGAGAATATGCCACAACAGAATGATGCATCACAGGAACTTAATCATTTTACTTTGCCCCAGAAGCAACCCCACAGTGATCATCTTCAGCCAGGGAAAGGAGATCAAAAACCTCTCCAGACTGGAATGTTGATGTCTGGAAAAAATCCAGTGTCAACACAGGAGCAGGACTTAACTCCTAAACCGGAAAGTGAATCTCAGTATGTAAAGCTTCAGAAGATGAGTAGTCAACAGGCAATGACCACGGAGCAGCCAAGCACCCCGATGAATCGCAGTAAACAAGTACCATTTGGTCTGTTACTACCTGTCTTAATTCCTCAACTTGATAAAGACAGAGCCATGCAACTTACAACTCTTTTTGGTAAACTGAAGAACAATGAAATCTCCAAAGATGCTTTTGTCCGACACATTAGAAGTGTTGTTGGGGACCAGATGCTCAAGATGGCAGTCCATAAAGTACAGACACAGCCTGCTCTTAAACAACAGTTGATGCCTCAGGCTTCATTGCAGCAGCAACCTCCTAGGATGCCATCTATTAGTGCAGGTGCTACCCAGTTCACTGATCCTCGTTTTGCAATTCATCAGAGAGGTGCGAACCCTTCCACAGACCCATCACACATTCCCACAGTTCACGTGCAGACTGATTCAAGCCATTCGGCCATTGAAAACAATGCCAAAAAATTGCGGGAAGCAGAACGTCAGTCAGATTCTCATGCAGTGCAAGTAAATCAGATGTCTTCTTCCAGTACAGGAGCGGCCAATCAAGAAAGGGAGCGGTCTTCAGTTGCTATGCAAGTACATAACAAGCAGCAGCAACAGCAACTGCACTACCCCCAATCATCCTTTGCCATGTATGGATCTACTGGTGGTAATTATCATCCATATCCAGGGACAAATGTCAATACAATGCCTCTCAAACAGCAATCACATGACTCTCATTTGAGGCAAATTCCTCAACATCAAAGCATGGGCTCAGCTCAGCCAGGTGGGGTGGAAGCCCAGGGAACAAACATCATGAGTGCGCCCAAATTGGAGAGGCAAAATTCTATGAATGACCCTAGCAGACAGCAGGGTGGATCTCTTCCTCACTTCACAAATAATTCGACTTTACAACAAAATCCAGTTCCTTGGCAGTCTTCAAATAAAGAGCAGATTTCTGGCCCTTTATCATCAATGCCTTATGTAAAACAGGAACCCATTGACCAGAGTGCTGAGCAGCAGCACAAGACCCCGCTATCTAATTCTCAGCGTTTGCCTTCTGCTTCTTCTGTGCAACATGAACAAGGAAATGCATCACCTGGAATTTCAATGGACGAGTCTTTAGAGAAGCAGTCTTCAAGAATGGGATTTTCAAGCACAGGGCCCTCTGGGAGCATGGTGCCTTTGTCATCTACAAGCACAGGGCCCTTGAATCCTGTTTCTTCTTCCACAACGATGCAAGCTGATCCTAATTTAAGCTCTCGGATACCTACTGGCACTTCTCCTGCTGGGATTAATAATAGAGTACCTGCAAAAAAGACTTCTGTTGGCCAAAAGAAGCCTAGTGAAGCACTTGGTTCTTCACCACCGCCTTCAAGTAAGAAGCAAAAAGTATCTGGGGCTTTTTTGGATCAAAGCATTGAACAACTCAATGATGTCACAGCTGTTAGTGGAGTTAATCTAAGGGAAGAGGAGGAGCAGCTGTTTTCTGGGCCCAAGGACGACGGTCGAGCTTCAGAAGCATCTCGAAGAGTTGTGCAAGAAGAAGAAGAAAGGCTGATTTTGCAGAAGACTCCCTTGCAGAAAAAATTGGCAGAAATCATGTTCAGATGTGGTTTGAAAAGTATAAGCAATGATGTGGAACGATGCTTGTCACTGTGCGTGGAGGAAAGAATGAGGGGGCTGATAAATAATTTGATAAGACTGTCAAAGCAGCGGGTTGATGCTGAGAAACCAAAACACCACACCATCATTACCTCTGATGTTCGGCAACAAATTTTAAATCAGAACAAGAAAGCTAAGGAAGAATGGGAAAAGAAGCAAGCTGAGGCAGAAAAGGTCCGGAAGCTTAATGAACCTGAGGTTAGCAATGGAGTTGACGGTGATAAAGACAGAGATGAAGGTCGTTCTAAATCATTTAAGGCAAACAAAGAAGAAGATGACAAAATGAGGACAACAGCAGCAAATGTTGCTGCCCGTGCTGCTGTTGGAGGAGATGACATGTTGTCAAAATGGCAACTTATGGCTGAGCAAGCTCGCCAAAAACGTGAAGGTGGGAGTGATGTGGCATCTGGGTCTCAGCCAGGTAAAGATGTAAATCGCAAGCTTACATCAGCAGCTGGAAGAATTATGAAGGACAATCAAGAAGTAGAAAAAAAGGGGGTGCAGCTCCTGTTTCTGCAGCTGGGTCCCATCTCATTTGTTTTGGTCTACAAGGGTCATGCAGGATGCCAATGGTGTTGCAACTTATGGACTGTTAGAAAATTTGGGAAAAACCAATTTATTGTGCCCCAGACGAGGGTGGCTCGTAGTATTTCTGTCAAGGATGTGATTTCTGTTCTGGAAAGGGAACCTCAAATGTCAAAGTCACCTCTGATATATCGCTTGTATGAGAGAAACCAATCTGACTCTGCGGGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000428 GO:0001650 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005730 GO:0005911 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006357 GO:0006366 GO:0006367 GO:0006725 GO:0006807 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009506 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010646 GO:0016070 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0023051 GO:0030054 GO:0030880 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044452 GO:0044464 GO:0044798 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0050789 GO:0050794 GO:0051059 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0055029 GO:0055044 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901796 GO:1902494 GO:1902531 GO:1902680 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05016 ko05168 map03022 map05016 map05168
Pfam Domains
Protein Families
RST TAF4

Protein Analysis

978

Amino Acids

107.94

Weight (kDa)

8.54

Isoelectric Point (pI)

62.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RST PF12174 195 - 259 4e-20 RCD1-SRO-TAF4 (RST) plant domain
TAF4 PF05236 648 - 967 2e-81 Transcription initiation factor TFIID component TAF4 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 848
Acc36I ACCTGC 2 cut(s) 848, 1831
AccB1I GGYRCC 1 cut(s) 1693
AccBSI CCGCTC 2 cut(s) 1070, 1090
AccI GTMKAC 1 cut(s) 2724
AccIII TCCGGA 1 cut(s) 2355
AclI AACGTT 1 cut(s) 307
AclWI GGATC 5 cut(s) 872, 1180, 1399, 1754, 1932
AcoI YGGCCR 3 cut(s) 972, 1071, 1843
AcsI RAATTY 6 cut(s) 237, 1263, 1357, 1617, 2289, 2777
AcuI CTGAAG 5 cut(s) 386, 512, 704, 1080, 2013
AcvI CACGTG 1 cut(s) 949
AfaI GTAC 6 cut(s) 226, 600, 774, 1063, 1113, 1821
AfiI CCNNNNNNNGG 6 cut(s) 96, 97, 413, 1202, 2811, 2812
AflIII ACRYGT 1 cut(s) 2514
AhdI GACNNNNNGTC 1 cut(s) 2518
AjnI CCWGG 7 cut(s) 213, 406, 1201, 1297, 1314, 1612, 2596
AjuI GAANNNNNNNTTGG 2 cut(s) 11, 43
Alw26I GTCTC 2 cut(s) 162, 2595
AlwI GGATC 5 cut(s) 872, 1180, 1399, 1754, 1932
AlwNI CAGNNNCTG 4 cut(s) 146, 1138, 2633, 2686
Aor13HI TCCGGA 1 cut(s) 2355
AoxI GGCC 9 cut(s) 75, 87, 972, 1071, 1474, 1676, 1718, 1843, 2006
ApaI GGGCCC 3 cut(s) 1680, 1722, 2010
ApoI RAATTY 6 cut(s) 237, 1263, 1357, 1617, 2289, 2777
AseI ATTAAT 1 cut(s) 1809
AspA2I CCTAGG 1 cut(s) 836
AspLEI GCGC 1 cut(s) 1339
AsuHPI GGTGA 5 cut(s) 122, 1602, 1869, 2408, 2874
AsuNHI GCTAGC 1 cut(s) 209
AvaII GGWCC 4 cut(s) 4, 742, 2353, 2702
AvrII CCTAGG 1 cut(s) 836
AxyI CCTNAGG 2 cut(s) 810, 2373
BaeGI GKGCMC 4 cut(s) 1680, 1722, 2010, 2805
BalI TGGCCA 1 cut(s) 1845
BanI GGYRCC 1 cut(s) 1693
BanII GRGCYC 4 cut(s) 1289, 1680, 1722, 2010
BarI GAAGNNNNNNTAC 2 cut(s) 157, 189
BbrPI CACGTG 1 cut(s) 949
BbsI GAAGAC 6 cut(s) 39, 1044, 1086, 1443, 1641, 2090
BbvCI CCTCAGC 1 cut(s) 2340
BccI CCATC 6 cut(s) 320, 751, 853, 934, 2270, 2714
BciT130I CCWGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
BciVI GTATCC 1 cut(s) 1773
BclI TGATCA 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 162, 2595
BfaI CTAG 5 cut(s) 180, 210, 837, 1374, 1857
BfmI CTRYAG 2 cut(s) 147, 2693
BfuAI ACCTGC 2 cut(s) 848, 1831
BfuI GTATCC 1 cut(s) 1773
BglI GCCNNNNNGGC 1 cut(s) 83
BlnI CCTAGG 1 cut(s) 836
BlpI GCTNAGC 4 cut(s) 1288, 1293, 1527, 2541
BmcAI AGTACT 1 cut(s) 226
Bme1390I CCNGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
Bme18I GGWCC 4 cut(s) 4, 742, 2353, 2702
BmeRI GACNNNNNGTC 1 cut(s) 2518
BmrFI CCNGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
BmrI ACTGGG 1 cut(s) 862
BmtI GCTAGC 1 cut(s) 213
BmuI ACTGGG 1 cut(s) 862
BoxI GACNNNNGTC 2 cut(s) 1211, 2019
BpiI GAAGAC 6 cut(s) 39, 1044, 1086, 1443, 1641, 2090
BplI GAGNNNNNCTC 2 cut(s) 2803, 2835
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 2 cut(s) 2313, 2340
Bpu1102I GCTNAGC 4 cut(s) 1288, 1293, 1527, 2541
BpuEI CTTGAG 2 cut(s) 144, 737
BsaAI YACGTR 1 cut(s) 949
BsaBI GATNNNNATC 1 cut(s) 2610
BsaI GGTCTC 1 cut(s) 2595
BsaJI CCNNGG 8 cut(s) 407, 558, 836, 1202, 1314, 1315, 1442, 2010
BsaWI WCCGGW 2 cut(s) 499, 2355
BsaXI ACNNNNNCTCC 2 cut(s) 1982, 2012
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 97, 413, 1202, 2811, 2812
Bse1I ACTGG 7 cut(s) 442, 464, 868, 1059, 1183, 1436, 1792
Bse21I CCTNAGG 2 cut(s) 810, 2373
Bse3DI GCAATG 4 cut(s) 558, 818, 2152, 2389
Bse8I GATNNNNATC 1 cut(s) 2610
BseAI TCCGGA 1 cut(s) 2355
BseBI CCWGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
BseDI CCNNGG 8 cut(s) 407, 558, 836, 1202, 1314, 1315, 1442, 2010
BseGI GGATG 8 cut(s) 49, 73, 223, 846, 1154, 1192, 2747, 2845
BseJI GATNNNNATC 1 cut(s) 2610
BseLI CCNNNNNNNGG 6 cut(s) 96, 97, 413, 1202, 2811, 2812
BseMI GCAATG 4 cut(s) 558, 818, 2152, 2389
BseNI ACTGG 7 cut(s) 442, 464, 868, 1059, 1183, 1436, 1792
BseRI GAGGAG 3 cut(s) 2000, 2003, 2520
BseSI GKGCMC 4 cut(s) 1680, 1722, 2010, 2805
BseYI CCCAGC 2 cut(s) 1802, 2698
BsgI GTGCAG 4 cut(s) 876, 971, 1124, 2699
Bsh1285I CGRYCG 1 cut(s) 2023
BshFI GGCC 9 cut(s) 77, 89, 974, 1073, 1476, 1678, 1720, 1845, 2008
BshNI GGYRCC 1 cut(s) 1693
BsiEI CGRYCG 1 cut(s) 2023
BsiSI CCGG 2 cut(s) 500, 2356
BslFI GGGAC 4 cut(s) 113, 755, 1219, 2688
BslI CCNNNNNNNGG 6 cut(s) 96, 97, 413, 1202, 2811, 2812
BsmAI GTCTC 2 cut(s) 162, 2595
BsmFI GGGAC 4 cut(s) 113, 755, 1219, 2688
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 9 cut(s) 77, 89, 974, 1073, 1476, 1678, 1720, 1845, 2008
Bso31I GGTCTC 1 cut(s) 2595
Bsp120I GGGCCC 3 cut(s) 1676, 1718, 2006
Bsp1286I GDGCHC 5 cut(s) 1289, 1680, 1722, 2010, 2805
Bsp13I TCCGGA 1 cut(s) 2355
Bsp143I GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
Bsp1720I GCTNAGC 4 cut(s) 1288, 1293, 1527, 2541
BspANI GGCC 9 cut(s) 77, 89, 974, 1073, 1476, 1678, 1720, 1845, 2008
BspEI TCCGGA 1 cut(s) 2355
BspHI TCATGA 1 cut(s) 1329
BspMAI CTGCAG 2 cut(s) 151, 2697
BspMI ACCTGC 2 cut(s) 848, 1831
BspOI GCTAGC 1 cut(s) 213
BspPI GGATC 5 cut(s) 872, 1180, 1399, 1754, 1932
BspT107I GGYRCC 1 cut(s) 1693
BspTNI GGTCTC 1 cut(s) 2595
BsrBI CCGCTC 2 cut(s) 1070, 1090
BsrDI GCAATG 4 cut(s) 558, 818, 2152, 2389
BsrI ACTGG 7 cut(s) 442, 464, 868, 1059, 1183, 1436, 1792
BssECI CCNNGG 8 cut(s) 407, 558, 836, 1202, 1314, 1315, 1442, 2010
BssMI GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
BssT1I CCWWGG 3 cut(s) 836, 1442, 2010
Bst2UI CCWGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
Bst4CI ACNGT 9 cut(s) 198, 392, 801, 943, 2021, 2172, 2220, 2396, 2770
Bst6I CTCTTC 3 cut(s) 1401, 1977, 2038
BstAPI GCANNNNNTGC 1 cut(s) 2483
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 7 cut(s) 211, 786, 1755, 2337, 2547, 2551, 2618
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 8 cut(s) 49, 73, 223, 846, 1154, 1192, 2747, 2845
BstHHI GCGC 1 cut(s) 1339
BstKTI GATC 7 cut(s) 397, 421, 880, 1175, 1394, 1762, 1927
BstMAI GTCTC 2 cut(s) 162, 2595
BstMBI GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
BstMCI CGRYCG 1 cut(s) 2023
BstNI CCWGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
BstNSI RCATGY 1 cut(s) 2518
BstPAI GACNNNNGTC 2 cut(s) 1211, 2019
BstSCI CCNGG 7 cut(s) 213, 406, 1201, 1297, 1314, 1612, 2596
BstSFI CTRYAG 2 cut(s) 147, 2693
BstSLI GKGCMC 4 cut(s) 1680, 1722, 2010, 2805
BstV2I GAAGAC 6 cut(s) 39, 1044, 1086, 1443, 1641, 2090
BstX2I RGATCY 2 cut(s) 1172, 1391
BstXI CCANNNNNNTGG 1 cut(s) 1443
BstYI RGATCY 2 cut(s) 1172, 1391
Bsu36I CCTNAGG 2 cut(s) 810, 2373
BsuI GTATCC 1 cut(s) 1773
BsuRI GGCC 9 cut(s) 77, 89, 974, 1073, 1476, 1678, 1720, 1845, 2008
BtgI CCRYGG 1 cut(s) 558
BtsCI GGATG 8 cut(s) 49, 73, 223, 846, 1154, 1192, 2747, 2845
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 5 cut(s) 397, 471, 873, 1038, 2168
BveI ACCTGC 2 cut(s) 848, 1831
Cac8I GCNNGC 7 cut(s) 211, 786, 1755, 2337, 2547, 2551, 2618
CaiI CAGNNNCTG 4 cut(s) 146, 1138, 2633, 2686
CciI TCATGA 1 cut(s) 1329
CfoI GCGC 1 cut(s) 1339
Csp6I GTAC 6 cut(s) 225, 599, 773, 1062, 1112, 1820
CviQI GTAC 6 cut(s) 225, 599, 773, 1062, 1112, 1820
DpnI GATC 7 cut(s) 396, 420, 879, 1174, 1393, 1761, 1926
DpnII GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
DraI TTTAAA 1 cut(s) 2295
DriI GACNNNNNGTC 1 cut(s) 2518
EaeI YGGCCR 3 cut(s) 972, 1071, 1843
Eam1104I CTCTTC 3 cut(s) 1401, 1977, 2038
Eam1105I GACNNNNNGTC 1 cut(s) 2518
EarI CTCTTC 3 cut(s) 1401, 1977, 2038
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 3 cut(s) 836, 1442, 2010
Eco147I AGGCCT 1 cut(s) 77
Eco24I GRGCYC 4 cut(s) 1289, 1680, 1722, 2010
Eco31I GGTCTC 1 cut(s) 2595
Eco47I GGWCC 4 cut(s) 4, 742, 2353, 2702
Eco57I CTGAAG 5 cut(s) 386, 512, 704, 1080, 2013
Eco72I CACGTG 1 cut(s) 949
Eco81I CCTNAGG 2 cut(s) 810, 2373
EcoO109I RGGNCCY 5 cut(s) 1676, 1677, 1718, 1719, 2702
EcoRII CCWGG 7 cut(s) 213, 406, 1201, 1297, 1314, 1612, 2596
EcoT14I CCWWGG 3 cut(s) 836, 1442, 2010
EcoT22I ATGCAT 3 cut(s) 57, 346, 1609
EcoT38I GRGCYC 4 cut(s) 1289, 1680, 1722, 2010
ErhI CCWWGG 3 cut(s) 836, 1442, 2010
FalI AAGNNNNNCTT 4 cut(s) 11, 43, 2075, 2107
FaqI GGGAC 4 cut(s) 113, 755, 1219, 2688
FauI CCCGC 4 cut(s) 993, 1554, 2223, 2920
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 394
FblI GTMKAC 1 cut(s) 2724
FokI GGATG 8 cut(s) 56, 80, 230, 853, 1141, 1179, 2754, 2852
FriOI GRGCYC 4 cut(s) 1289, 1680, 1722, 2010
FspBI CTAG 5 cut(s) 180, 210, 837, 1374, 1857
GlaI GCGC 1 cut(s) 1338
GsaI CCCAGC 2 cut(s) 1806, 2702
GsuI CTGGAG 1 cut(s) 416
HaeIII GGCC 9 cut(s) 77, 89, 974, 1073, 1476, 1678, 1720, 1845, 2008
HapII CCGG 2 cut(s) 500, 2356
HhaI GCGC 1 cut(s) 1339
Hin6I GCGC 1 cut(s) 1337
HinP1I GCGC 1 cut(s) 1337
HincII GTYRAC 3 cut(s) 471, 545, 2392
HindII GTYRAC 3 cut(s) 471, 545, 2392
HindIII AAGCTT 3 cut(s) 524, 2360, 2618
HpaII CCGG 2 cut(s) 500, 2356
HphI GGTGA 5 cut(s) 122, 1602, 1869, 2408, 2874
Hpy166II GTNNAC 8 cut(s) 471, 545, 593, 680, 873, 946, 2392, 2725
Hpy8I GTNNAC 8 cut(s) 471, 545, 593, 680, 873, 946, 2392, 2725
Hpy99I CGWCG 2 cut(s) 13, 2021
HpyAV CCTTC 7 cut(s) 101, 924, 1581, 1896, 2408, 2558, 2641
HpyCH4III ACNGT 9 cut(s) 198, 392, 801, 943, 2021, 2172, 2220, 2396, 2770
HpyCH4IV ACGT 4 cut(s) 307, 948, 1012, 2560
HpySE526I ACGT 4 cut(s) 307, 948, 1012, 2560
HspAI GCGC 1 cut(s) 1337
KflI GGGWCCC 1 cut(s) 2702
Kpn2I TCCGGA 1 cut(s) 2355
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
LmnI GCTCC 7 cut(s) 478, 562, 1067, 1087, 1686, 1990, 2688
MaeI CTAG 5 cut(s) 180, 210, 837, 1374, 1857
MaeII ACGT 4 cut(s) 307, 948, 1012, 2560
MaeIII GTNAC 4 cut(s) 612, 1951, 2166, 2880
MalI GATC 7 cut(s) 396, 420, 879, 1174, 1393, 1761, 1926
MbiI CCGCTC 2 cut(s) 1070, 1090
MboI GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
MflI RGATCY 2 cut(s) 1172, 1391
MhlI GDGCHC 5 cut(s) 1289, 1680, 1722, 2010, 2805
MlsI TGGCCA 1 cut(s) 1845
MluNI TGGCCA 1 cut(s) 1845
MlyI GAGTC 5 cut(s) 137, 1241, 1640, 2080, 2915
MmeI TCCRAC 2 cut(s) 742, 2482
Mox20I TGGCCA 1 cut(s) 1845
Mph1103I ATGCAT 3 cut(s) 57, 346, 1609
MroI TCCGGA 1 cut(s) 2355
MscI TGGCCA 1 cut(s) 1845
MslI CAYNNNNRTG 2 cut(s) 578, 1748
Msp20I TGGCCA 1 cut(s) 1845
MspA1I CMGCKG 7 cut(s) 130, 265, 1958, 1996, 2230, 2633, 2698
MspI CCGG 2 cut(s) 500, 2356
MspR9I CCNGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
NdeI CATATG 1 cut(s) 283
NdeII GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
NheI GCTAGC 1 cut(s) 209
NmuCI GTSAC 3 cut(s) 1951, 2166, 2880
NsiI ATGCAT 3 cut(s) 57, 346, 1609
NspI RCATGY 1 cut(s) 2518
PagI TCATGA 1 cut(s) 1329
PaqCI CACCTGC 1 cut(s) 848
PasI CCCWGGG 1 cut(s) 1315
PceI AGGCCT 1 cut(s) 77
PciI ACATGT 1 cut(s) 2514
PcsI WCGNNNNNNNCGW 1 cut(s) 2557
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 6 cut(s) 137, 509, 583, 959, 1022, 1726
PleI GAGTC 5 cut(s) 137, 1241, 1639, 2080, 2915
PmaCI CACGTG 1 cut(s) 949
PmlI CACGTG 1 cut(s) 949
PpsI GAGTC 5 cut(s) 137, 1241, 1639, 2080, 2915
Ppu21I YACGTR 1 cut(s) 949
PpuMI RGGWCCY 1 cut(s) 2702
PscI ACATGT 1 cut(s) 2514
PshAI GACNNNNGTC 2 cut(s) 1211, 2019
PshBI ATTAAT 1 cut(s) 1809
Psp1406I AACGTT 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 2702
Psp6I CCWGG 7 cut(s) 213, 406, 1201, 1297, 1314, 1612, 2596
PspCI CACGTG 1 cut(s) 949
PspFI CCCAGC 2 cut(s) 1802, 2698
PspGI CCWGG 7 cut(s) 213, 406, 1201, 1297, 1314, 1612, 2596
PspOMI GGGCCC 3 cut(s) 1676, 1718, 2006
PspPPI RGGWCCY 1 cut(s) 2702
PstI CTGCAG 2 cut(s) 151, 2697
PstNI CAGNNNCTG 4 cut(s) 146, 1138, 2633, 2686
PsuI RGATCY 2 cut(s) 1172, 1391
PvuII CAGCTG 4 cut(s) 1958, 1996, 2633, 2698
RsaI GTAC 6 cut(s) 226, 600, 774, 1063, 1113, 1821
RsaNI GTAC 6 cut(s) 225, 599, 773, 1062, 1112, 1820
RseI CAYNNNNRTG 2 cut(s) 578, 1748
Sau3AI GATC 7 cut(s) 394, 418, 877, 1172, 1391, 1759, 1924
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 5 cut(s) 137, 1241, 1640, 2080, 2915
ScrFI CCNGG 7 cut(s) 215, 408, 1203, 1299, 1316, 1614, 2598
SduI GDGCHC 5 cut(s) 1289, 1680, 1722, 2010, 2805
SfcI CTRYAG 2 cut(s) 147, 2693
SinI GGWCC 4 cut(s) 4, 742, 2353, 2702
SmiMI CAYNNNNRTG 2 cut(s) 578, 1748
SmlI CTYRAG 2 cut(s) 159, 752
SmoI CTYRAG 2 cut(s) 159, 752
SseBI AGGCCT 1 cut(s) 77
SspI AATATT 1 cut(s) 256
SspMI CTAG 5 cut(s) 180, 210, 837, 1374, 1857
StuI AGGCCT 1 cut(s) 77
StyD4I CCNGG 7 cut(s) 213, 406, 1201, 1297, 1314, 1612, 2596
StyI CCWWGG 3 cut(s) 836, 1442, 2010
TaaI ACNGT 9 cut(s) 198, 392, 801, 943, 2021, 2172, 2220, 2396, 2770
TaiI ACGT 4 cut(s) 310, 951, 1015, 2563
TaqI TCGA 4 cut(s) 11, 1418, 2023, 2041
TatI WGTACW 4 cut(s) 224, 772, 1061, 1111
TauI GCSGC 3 cut(s) 89, 268, 1073
TfiI GAWTC 6 cut(s) 137, 509, 583, 959, 1022, 1726
TscAI CASTG 5 cut(s) 397, 471, 880, 1038, 2175
TseFI GTSAC 3 cut(s) 1951, 2166, 2880
Tsp45I GTSAC 3 cut(s) 1951, 2166, 2880
TspGWI ACGGA 1 cut(s) 575
TspRI CASTG 5 cut(s) 397, 471, 880, 1038, 2175
VpaK11BI GGWCC 4 cut(s) 4, 742, 2353, 2702
VspI ATTAAT 1 cut(s) 1809
XapI RAATTY 6 cut(s) 237, 1263, 1357, 1617, 2289, 2777
XceI RCATGY 1 cut(s) 2518
XmaJI CCTAGG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 2724
XspI CTAG 5 cut(s) 180, 210, 837, 1374, 1857
ZrmI AGTACT 1 cut(s) 226
Zsp2I ATGCAT 3 cut(s) 57, 346, 1609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.