MD06G1165200.v1.1

50S ribosomal protein L19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003350
Physical Location & Seq
Forward (+)
30541081 .. 30543040
1960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1165200.v1.1.491

Sequence Viewer

Length: 612 bp
ATGCTGTGTACTATATTGTACCAGACTACAACTAGCTCTCCAAGTGAGACATCAACTTGTTCGTGTAGTTCCAAGTTATTCAAGAGTGCTCGACCAGGATTTTGTCGATGTGCGGCAGTTTTACCTCCTTTCCCAAGCAGGTGCATTACAACAGGGGCAAATCCTGTTCAATCAGCTACTCAGGATTCAGGAATGTATGAGTCTGATGTAGCTCCTCCCATCAAATTCAAGAGGCTTGATAAAACTGCTCACCACATAATGCAGGCATGCCTCTATCTGTTGATTCTAGATAAGGAAGCTGTAGAGGAAGTGAGGACACAGAGAGAAATACCGGATGTAAGGCCTGGGTATATGGTGCAACTCAGAGTGGAAGTACCCGACAACAAGCGACGTGTTTCAGTCGTGAAAGGCGTCGTGATAGCTAGACGTAATGCTGGTCTAAACTCTACAATTAGAATCAGGAGGCAAGTGGCTGGGGTCGGGGTTGAATCTCTCTTTCCACTGTATTCGCCTAATATAAAGGAGATTAAGGTGCTGGACAAGAAGAAAGTGCGGAGGGCGAAGCTGTACTATGTCAGGGACAAAATCAATGCATTTAAGAAGCAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L19

Protein Analysis

204

Amino Acids

22.79

Weight (kDa)

9.9

Isoelectric Point (pI)

65.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 100 - 196 8.3e-29 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 129
Acc36I ACCTGC 1 cut(s) 129
AciI CCGC 2 cut(s) 113, 553
AcsI RAATTY 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 4 cut(s) 10, 20, 375, 569
AflIII ACRYGT 1 cut(s) 391
AgsI TTSAA 4 cut(s) 82, 170, 229, 488
AjiI CACGTC 1 cut(s) 392
AjnI CCWGG 2 cut(s) 94, 343
AluBI AGCT 6 cut(s) 36, 176, 212, 299, 422, 565
AluI AGCT 6 cut(s) 36, 176, 212, 299, 422, 565
Alw21I GWGCWC 1 cut(s) 91
Alw26I GTCTC 1 cut(s) 41
AoxI GGCC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 224
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AsuHPI GGTGA 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 91
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 2 cut(s) 96, 345
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 3 cut(s) 33, 287, 423
BfmI CTRYAG 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 129
BisI GCNGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 115
Bme1390I CCNGG 2 cut(s) 96, 345
BmgBI CACGTC 1 cut(s) 392
BmrFI CCNGG 2 cut(s) 96, 345
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
BseBI CCWGG 2 cut(s) 96, 345
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 1 cut(s) 340
BseMII CTCAG 2 cut(s) 194, 376
BseRI GAGGAG 1 cut(s) 204
BseYI CCCAGC 1 cut(s) 473
BshFI GGCC 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 91
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 593
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 1 cut(s) 593
BsnI GGCC 1 cut(s) 343
Bsp1286I GDGCHC 1 cut(s) 91
BspACI CCGC 2 cut(s) 113, 553
BspANI GGCC 1 cut(s) 343
BspCNI CTCAG 2 cut(s) 193, 375
BspMI ACCTGC 1 cut(s) 129
BssECI CCNNGG 1 cut(s) 344
BssNI GRCGYC 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 96, 345
Bst4CI ACNGT 1 cut(s) 504
BstACI GRCGYC 1 cut(s) 411
BstC8I GCNNGC 2 cut(s) 264, 268
BstDEI CTNAG 2 cut(s) 180, 362
BstF5I GGATG 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 41
BstNI CCWGG 2 cut(s) 96, 345
BstNSI RCATGY 1 cut(s) 270
BstSCI CCNGG 2 cut(s) 94, 343
BstSFI CTRYAG 1 cut(s) 300
BsuRI GGCC 1 cut(s) 343
BtrI CACGTC 1 cut(s) 392
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 500
BveI ACCTGC 1 cut(s) 129
Cac8I GCNNGC 2 cut(s) 264, 268
CseI GACGC 1 cut(s) 400
Csp6I GTAC 4 cut(s) 9, 19, 374, 568
CviAII CATG 1 cut(s) 267
CviJI RGCY 9 cut(s) 36, 176, 212, 235, 299, 343, 422, 473, 565
CviKI_1 RGCY 9 cut(s) 36, 176, 212, 235, 299, 343, 422, 473, 565
CviQI GTAC 4 cut(s) 9, 19, 374, 568
DdeI CTNAG 2 cut(s) 180, 362
Eco147I AGGCCT 1 cut(s) 343
EcoRII CCWGG 2 cut(s) 94, 343
EcoT22I ATGCAT 1 cut(s) 595
FaeI CATG 1 cut(s) 270
FaiI YATR 8 cut(s) 14, 198, 257, 268, 351, 353, 518, 573
FaqI GGGAC 1 cut(s) 593
FatI CATG 1 cut(s) 266
Fnu4HI GCNGC 1 cut(s) 114
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 114
FspBI CTAG 3 cut(s) 33, 287, 423
GluI GCNGC 1 cut(s) 114
GsaI CCCAGC 1 cut(s) 477
HaeIII GGCC 1 cut(s) 343
HapII CCGG 1 cut(s) 332
HgaI GACGC 1 cut(s) 400
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 1 cut(s) 270
HinfI GANTC 5 cut(s) 185, 200, 283, 456, 488
HpaII CCGG 1 cut(s) 332
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 2 cut(s) 205, 365
Hpy188III TCNNGA 8 cut(s) 82, 182, 189, 229, 287, 403, 415, 460
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 2 cut(s) 393, 416
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4IV ACGT 2 cut(s) 391, 427
HpyCH4V TGCA 4 cut(s) 144, 262, 358, 593
HpyF3I CTNAG 2 cut(s) 180, 362
HpySE526I ACGT 2 cut(s) 391, 427
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 1 cut(s) 270
LmnI GCTCC 1 cut(s) 217
MaeI CTAG 3 cut(s) 33, 287, 423
MaeII ACGT 2 cut(s) 391, 427
MboII GAAGA 1 cut(s) 556
MhlI GDGCHC 1 cut(s) 91
MluCI AATT 2 cut(s) 224, 450
MlyI GAGTC 1 cut(s) 209
MnlI CCTC 8 cut(s) 135, 225, 225, 281, 298, 306, 456, 549
Mph1103I ATGCAT 1 cut(s) 595
MseI TTAA 2 cut(s) 528, 597
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 2 cut(s) 96, 345
MvaI CCWGG 2 cut(s) 96, 345
NlaIII CATG 1 cut(s) 270
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 270
PaeI GCATGC 1 cut(s) 270
PaqCI CACCTGC 1 cut(s) 129
PceI AGGCCT 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PfeI GAWTC 4 cut(s) 185, 283, 456, 488
PkrI GCNGC 1 cut(s) 115
PleI GAGTC 1 cut(s) 208
PpsI GAGTC 1 cut(s) 208
Psp6I CCWGG 2 cut(s) 94, 343
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 2 cut(s) 94, 343
RsaI GTAC 4 cut(s) 10, 20, 375, 569
RsaNI GTAC 4 cut(s) 9, 19, 374, 568
SaqAI TTAA 2 cut(s) 528, 597
SatI GCNGC 1 cut(s) 114
SchI GAGTC 1 cut(s) 209
ScrFI CCNGG 2 cut(s) 96, 345
SduI GDGCHC 1 cut(s) 91
SfcI CTRYAG 1 cut(s) 300
SphI GCATGC 1 cut(s) 270
Sse9I AATT 2 cut(s) 224, 450
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 2 cut(s) 113, 553
SspMI CTAG 3 cut(s) 33, 287, 423
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 2 cut(s) 94, 343
TaaI ACNGT 1 cut(s) 504
TaiI ACGT 2 cut(s) 394, 430
TaqI TCGA 2 cut(s) 91, 106
TasI AATT 2 cut(s) 224, 450
TatI WGTACW 2 cut(s) 8, 567
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 4 cut(s) 185, 283, 456, 488
Tru1I TTAA 2 cut(s) 528, 597
Tru9I TTAA 2 cut(s) 528, 597
TscAI CASTG 1 cut(s) 507
TspRI CASTG 1 cut(s) 507
XapI RAATTY 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 286
XceI RCATGY 1 cut(s) 270
XspI CTAG 3 cut(s) 33, 287, 423
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.