pycom06g14770

50S ribosomal protein L19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0003350
Physical Location & Seq
Forward (+)
19430753 .. 19433124
2372 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g14770.1

Sequence Viewer

Length: 810 bp
ATGCAGTCTTTAAGCGCGAGCATCCGATTGGTCCGAAGACAGAGAATAGACAGCTCAAAAATTGCTCATTTTAGATCAATCGGAGCTTACCAATGGAGCAGCGGTTCTGGGTTTAGCTCGTCGGCGTCGGATCGTTTGGCGTCTGCCAAGAAATTGGGGGATTCTTCGGGGTTGGGGTCTCTGAAAAGTTCCTTGTTTCAGTCAATTTCCAAGTTTTCGTATCGGTCTTCGGCCTCGTTCACTACGACTAGCTCTCCAAGTGAGACATCAACTTGTTCGTGTAGTTCCAAGTTAGTCAAGAGTGCTCGACCAGGATTTTGTCGATGTGCGGCAGTTTTGCCTCCTTTCCCAAGCAGGTGCATTACAACAGGGGCAAATCCTGTTCAATCAGCTACTCAAGATTCAGGAATGTATGAGTCTGATGTAGCTCCTCGCATCAAATTCAAGAGGCTTGATAAAACTGCTCACCACATAATGCAGATTCTAGATAAGGAAGCTGTAGAGGAAGTGAGGACACAGAGAGAAATACCGGATGTAAGGCCCGGGTACATGGTGCAACTCAGAGTGGAAGTACCCGACAACAAGCGACGTGTTTCAGTCGTGAAAGGCGTTGTGATAGCTAGACGTAATGCTGGTCTAAACTCTACAATTAGAATCAGGAGGCAAGTGGCCGGGGTCGGGGTTGAATCTCTCTTTCCACTGTATTCGCCTAATATAAAGGAGATTAAGGTGCTGGACAAGAAGAAAGTGCGGAGGGCGAAGCTGTACTATGTCAGGGACAAAATCAATGCATTTAAGAAGCAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L19

Protein Analysis

270

Amino Acids

29.74

Weight (kDa)

10.45

Isoelectric Point (pI)

59.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 166 - 262 1.3e-28 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 345
AccII CGCG 1 cut(s) 17
AciI CCGC 3 cut(s) 102, 329, 751
AclWI GGATC 1 cut(s) 138
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 1 cut(s) 440
AcyI GRCGYC 2 cut(s) 125, 140
AfaI GTAC 3 cut(s) 548, 573, 767
AfiI CCNNNNNNNGG 1 cut(s) 678
AflIII ACRYGT 1 cut(s) 589
AgsI TTSAA 3 cut(s) 386, 445, 686
AjiI CACGTC 1 cut(s) 590
AjnI CCWGG 1 cut(s) 310
AloI GAACNNNNNNTCC 2 cut(s) 88, 120
AluBI AGCT 9 cut(s) 54, 86, 117, 252, 392, 428, 497, 620, 763
AluI AGCT 9 cut(s) 54, 86, 117, 252, 392, 428, 497, 620, 763
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 2 cut(s) 183, 257
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 542
AoxI GGCC 3 cut(s) 231, 539, 669
ApeKI GCWGC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 440
ArsI GACNNNNNNTTYG 2 cut(s) 300, 332
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 2 cut(s) 31, 540
AsuC2I CCSGG 3 cut(s) 543, 544, 673
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 542
AvaII GGWCC 1 cut(s) 31
BbsI GAAGAC 2 cut(s) 43, 219
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 111
BciT130I CCWGG 1 cut(s) 312
BcnI CCSGG 3 cut(s) 543, 544, 673
BcoDI GTCTC 2 cut(s) 183, 257
BfaI CTAG 3 cut(s) 249, 485, 621
BfmI CTRYAG 1 cut(s) 498
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 2 cut(s) 100, 330
BlsI GCNGC 2 cut(s) 101, 331
Bme1390I CCNGG 4 cut(s) 312, 543, 544, 673
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 542
BmgBI CACGTC 1 cut(s) 590
BmgT120I GGNCC 2 cut(s) 31, 540
BmrFI CCNGG 4 cut(s) 312, 543, 544, 673
BmsI GCATC 2 cut(s) 30, 444
BpiI GAAGAC 2 cut(s) 43, 219
BpuEI CTTGAG 1 cut(s) 381
BpuMI CCSGG 3 cut(s) 543, 544, 673
BsaHI GRCGYC 2 cut(s) 125, 140
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 542, 672
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 678
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 2 cut(s) 542, 672
BseGI GGATG 2 cut(s) 21, 538
BseLI CCNNNNNNNGG 1 cut(s) 678
BseMII CTCAG 1 cut(s) 574
BseRI GAGGAG 1 cut(s) 420
BseXI GCAGC 1 cut(s) 111
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 3 cut(s) 233, 541, 671
BsiHKAI GWGCWC 1 cut(s) 307
BsiHKCI CYCGRG 1 cut(s) 542
BsiSI CCGG 3 cut(s) 530, 543, 672
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 1 cut(s) 678
BsmAI GTCTC 2 cut(s) 183, 257
BsmFI GGGAC 1 cut(s) 791
BsnI GGCC 3 cut(s) 233, 541, 671
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 542
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 2 cut(s) 74, 130
BspACI CCGC 3 cut(s) 102, 329, 751
BspANI GGCC 3 cut(s) 233, 541, 671
BspCNI CTCAG 1 cut(s) 573
BspFNI CGCG 1 cut(s) 17
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 1 cut(s) 138
BspTNI GGTCTC 1 cut(s) 183
BssECI CCNNGG 2 cut(s) 542, 672
BssMI GATC 2 cut(s) 74, 130
BssNI GRCGYC 2 cut(s) 125, 140
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 1 cut(s) 702
BstACI GRCGYC 2 cut(s) 125, 140
BstC8I GCNNGC 1 cut(s) 19
BstDEI CTNAG 1 cut(s) 560
BstF5I GGATG 2 cut(s) 21, 538
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 1 cut(s) 17
BstKTI GATC 2 cut(s) 77, 133
BstMAI GTCTC 2 cut(s) 183, 257
BstMBI GATC 2 cut(s) 74, 130
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 4 cut(s) 310, 541, 542, 671
BstSFI CTRYAG 1 cut(s) 498
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 111
BstV2I GAAGAC 2 cut(s) 43, 219
BstXI CCANNNNNNTGG 1 cut(s) 154
BsuRI GGCC 3 cut(s) 233, 541, 671
BtrI CACGTC 1 cut(s) 590
BtsCI GGATG 2 cut(s) 21, 538
BtsIMutI CAGTG 1 cut(s) 698
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 1 cut(s) 19
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 2 cut(s) 31, 540
Cfr9I CCCGGG 1 cut(s) 542
CseI GACGC 2 cut(s) 114, 129
Csp6I GTAC 3 cut(s) 547, 572, 766
CviAII CATG 1 cut(s) 550
CviQI GTAC 3 cut(s) 547, 572, 766
DdeI CTNAG 1 cut(s) 560
DpnI GATC 2 cut(s) 76, 132
DpnII GATC 2 cut(s) 74, 130
EaeI YGGCCR 1 cut(s) 669
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 542
EcoRII CCWGG 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 793
FaeI CATG 1 cut(s) 553
FaiI YATR 5 cut(s) 414, 473, 551, 716, 771
FaqI GGGAC 1 cut(s) 791
FatI CATG 1 cut(s) 549
Fnu4HI GCNGC 2 cut(s) 100, 330
FokI GGATG 2 cut(s) 8, 545
Fsp4HI GCNGC 2 cut(s) 100, 330
FspBI CTAG 3 cut(s) 249, 485, 621
GlaI GCGC 1 cut(s) 16
GluI GCNGC 2 cut(s) 100, 330
HaeIII GGCC 3 cut(s) 233, 541, 671
HapII CCGG 3 cut(s) 530, 543, 672
HgaI GACGC 2 cut(s) 114, 129
HhaI GCGC 1 cut(s) 17
Hin1I GRCGYC 2 cut(s) 125, 140
Hin1II CATG 1 cut(s) 553
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HinfI GANTC 6 cut(s) 161, 401, 416, 481, 654, 686
HpaII CCGG 3 cut(s) 530, 543, 672
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 7 cut(s) 26, 35, 83, 130, 183, 421, 563
Hpy188III TCNNGA 7 cut(s) 298, 398, 405, 445, 485, 601, 658
Hpy8I GTNNAC 1 cut(s) 240
Hpy99I CGWCG 3 cut(s) 124, 130, 591
HpyCH4III ACNGT 1 cut(s) 702
HpyCH4IV ACGT 2 cut(s) 589, 625
HpyCH4V TGCA 5 cut(s) 4, 360, 478, 556, 791
HpyF3I CTNAG 1 cut(s) 560
HpySE526I ACGT 2 cut(s) 589, 625
Hsp92I GRCGYC 2 cut(s) 125, 140
Hsp92II CATG 1 cut(s) 553
HspAI GCGC 1 cut(s) 15
Kzo9I GATC 2 cut(s) 74, 130
LmnI GCTCC 3 cut(s) 83, 96, 433
Lsp1109I GCAGC 1 cut(s) 111
LweI GCATC 2 cut(s) 30, 444
MaeI CTAG 3 cut(s) 249, 485, 621
MaeII ACGT 2 cut(s) 589, 625
MalI GATC 2 cut(s) 76, 132
MboI GATC 2 cut(s) 74, 130
MboII GAAGA 4 cut(s) 48, 156, 219, 754
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 5 cut(s) 60, 152, 204, 440, 648
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 8 cut(s) 244, 351, 441, 441, 496, 504, 654, 747
Mph1103I ATGCAT 1 cut(s) 793
MseI TTAA 3 cut(s) 11, 726, 795
MspA1I CMGCKG 1 cut(s) 102
MspI CCGG 3 cut(s) 530, 543, 672
MspR9I CCNGG 4 cut(s) 312, 543, 544, 673
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 17
NciI CCSGG 3 cut(s) 543, 544, 673
NdeII GATC 2 cut(s) 74, 130
NlaIII CATG 1 cut(s) 553
NsiI ATGCAT 1 cut(s) 793
PaqCI CACCTGC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 2 cut(s) 242, 606
PfeI GAWTC 5 cut(s) 161, 401, 481, 654, 686
PkrI GCNGC 2 cut(s) 101, 331
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspPI GGNCC 2 cut(s) 31, 540
RsaI GTAC 3 cut(s) 548, 573, 767
RsaNI GTAC 3 cut(s) 547, 572, 766
SaqAI TTAA 3 cut(s) 11, 726, 795
SatI GCNGC 2 cut(s) 100, 330
Sau3AI GATC 2 cut(s) 74, 130
Sau96I GGNCC 2 cut(s) 31, 540
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 4 cut(s) 312, 543, 544, 673
SduI GDGCHC 1 cut(s) 307
SfaNI GCATC 2 cut(s) 30, 444
SfcI CTRYAG 1 cut(s) 498
SinI GGWCC 1 cut(s) 31
SmaI CCCGGG 1 cut(s) 544
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 5 cut(s) 60, 152, 204, 440, 648
SsiI CCGC 3 cut(s) 102, 329, 751
SspMI CTAG 3 cut(s) 249, 485, 621
StyD4I CCNGG 4 cut(s) 310, 541, 542, 671
TaaI ACNGT 1 cut(s) 702
TaiI ACGT 2 cut(s) 592, 628
TaqI TCGA 2 cut(s) 307, 322
TaqII GACCGA 1 cut(s) 213
TasI AATT 5 cut(s) 60, 152, 204, 440, 648
TatI WGTACW 1 cut(s) 765
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 5 cut(s) 161, 401, 481, 654, 686
Tru1I TTAA 3 cut(s) 11, 726, 795
Tru9I TTAA 3 cut(s) 11, 726, 795
TscAI CASTG 1 cut(s) 705
TseI GCWGC 1 cut(s) 99
TspMI CCCGGG 1 cut(s) 542
TspRI CASTG 1 cut(s) 705
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 1 cut(s) 440
XbaI TCTAGA 1 cut(s) 484
XmaI CCCGGG 1 cut(s) 542
XspI CTAG 3 cut(s) 249, 485, 621
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.