MD14G1170700.v1.1

50S ribosomal protein L19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0003350
Physical Location & Seq
Forward (+)
26492590 .. 26495388
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1170700.v1.1.491

Sequence Viewer

Length: 813 bp
ATGCAATCTTTAAGTGCGAGCATCCGATTGGTCCGAAGGCAAAGGGTCGACAGCTCAAAAATTGCTCATTTCAGATCGATTGGAGCTTACCAATTGAGTGGAGGTTCTGGGTTTAGTTCGTTGGCGTCGGATCGTTTGGCCTCTGCCAAGAAATTGGGGGATTTATCGGGTTTTGGGTCTTCGAAGAGTTCCTTGTTTCAGTCAGTTTCCAAGTTTTCGTATCGATCTTCGGCCCCGTTCACAACAACGAGCTCTCAAAATGCGGCCCCAGCTTCCTGTAATTCCAGCTTATTCAAGAGTGCTCGACTAAGCTCTTTTCGATGTGCGGCAGTTTCACCTCCTTTCCCGAGCAGGTGCATTGCAACAGGGGGAAATTCTGTTCAGTCAGCCACTCAAGATTCAGGAATGTCTGTCTCTGATGTAGCTCCTTGCATCAAATTCAAGAGGCTTGATAAAACTGCCCACCACATAATGCAGGCATGTATTCTAGATAAGGAAGCTTTAGAGGAAGTGAAGGCGCAGAGAGAAATACCAGACGTTAGGCCTGGTTATATGGTGCAACTCAGAGTGGAAGTACCCGACAACAAGCGACGTGTTTCAGTCGTGAAAGGTGTTGTGATTGCTAGACGTAATGCTGGTCTAAACACTACAATTAGAATCAGGAGGCAAGTGGCTGGAGTCGGGGTTGAATCTCTCTTCCCATTGTATTCGCCTAACATAAAGGAAATTAAGGTGCTGGACAAGAAGAAAGTGCGGAGGGCCAAGCTGTACTATGTCAGGGACAAAATCAATGCATTCAAGAAGCAATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L19

Protein Analysis

271

Amino Acids

29.5

Weight (kDa)

10.51

Isoelectric Point (pI)

60.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L19 PF01245 167 - 263 1.8e-28 Ribosomal protein L19
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 342
Acc36I ACCTGC 1 cut(s) 342
AccI GTMKAC 1 cut(s) 48
AciI CCGC 3 cut(s) 263, 326, 754
AclWI GGATC 1 cut(s) 138
AcsI RAATTY 2 cut(s) 373, 437
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 2 cut(s) 576, 770
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 4 cut(s) 295, 442, 689, 799
AjiI CACGTC 1 cut(s) 593
AjnI CCWGG 1 cut(s) 544
AloI GAACNNNNNNTCC 2 cut(s) 363, 395
AluBI AGCT 9 cut(s) 54, 86, 252, 272, 288, 312, 425, 500, 766
AluI AGCT 9 cut(s) 54, 86, 252, 272, 288, 312, 425, 500, 766
Alw21I GWGCWC 2 cut(s) 254, 304
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 346
AoxI GGCC 5 cut(s) 138, 231, 264, 542, 759
ApoI RAATTY 2 cut(s) 373, 437
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 4 cut(s) 31, 232, 265, 759
AsuHPI GGTGA 1 cut(s) 327
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 346
AvaII GGWCC 1 cut(s) 31
BanII GRGCYC 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 254, 304
BciT130I CCWGG 1 cut(s) 546
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 2 cut(s) 488, 624
BfuAI ACCTGC 1 cut(s) 342
BisI GCNGC 2 cut(s) 264, 327
BlsI GCNGC 2 cut(s) 265, 328
Bme1390I CCNGG 1 cut(s) 546
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 346
BmgBI CACGTC 1 cut(s) 593
BmgT120I GGNCC 4 cut(s) 31, 232, 265, 759
BmiI GGNNCC 2 cut(s) 234, 267
BmrFI CCNGG 1 cut(s) 546
BmsI GCATC 2 cut(s) 30, 441
BpiI GAAGAC 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 696
Bpu14I TTCGAA 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 378
Bsa29I ATCGAT 2 cut(s) 77, 223
BsaHI GRCGYC 1 cut(s) 125
BsaXI ACNNNNNCTCC 2 cut(s) 669, 699
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 546
BseCI ATCGAT 2 cut(s) 77, 223
BseGI GGATG 1 cut(s) 21
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 1 cut(s) 577
BseYI CCCAGC 1 cut(s) 268
BshFI GGCC 5 cut(s) 140, 233, 266, 544, 761
BshVI ATCGAT 2 cut(s) 77, 223
BsiHKAI GWGCWC 2 cut(s) 254, 304
BsiHKCI CYCGRG 1 cut(s) 346
BslFI GGGAC 1 cut(s) 794
BsmAI GTCTC 1 cut(s) 418
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 1 cut(s) 794
BsnI GGCC 5 cut(s) 140, 233, 266, 544, 761
BsoBI CYCGRG 1 cut(s) 346
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 254, 304
Bsp143I GATC 3 cut(s) 74, 130, 224
BspACI CCGC 3 cut(s) 263, 326, 754
BspANI GGCC 5 cut(s) 140, 233, 266, 544, 761
BspCNI CTCAG 1 cut(s) 576
BspDI ATCGAT 2 cut(s) 77, 223
BspLI GGNNCC 2 cut(s) 234, 267
BspMI ACCTGC 1 cut(s) 342
BspPI GGATC 1 cut(s) 138
BspT104I TTCGAA 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 357
BssMI GATC 3 cut(s) 74, 130, 224
BssNI GRCGYC 1 cut(s) 125
Bst2UI CCWGG 1 cut(s) 546
Bst6I CTCTTC 2 cut(s) 179, 701
BstACI GRCGYC 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 19, 477
BstDEI CTNAG 2 cut(s) 308, 563
BstF5I GGATG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 3 cut(s) 77, 133, 227
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 3 cut(s) 74, 130, 224
BstMWI GCNNNNNNNGC 1 cut(s) 269
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 544
BstV2I GAAGAC 1 cut(s) 171
BstXI CCANNNNNNTGG 2 cut(s) 98, 154
Bsu15I ATCGAT 2 cut(s) 77, 223
BsuRI GGCC 5 cut(s) 140, 233, 266, 544, 761
BsuTUI ATCGAT 2 cut(s) 77, 223
BtrI CACGTC 1 cut(s) 593
BtsCI GGATG 1 cut(s) 21
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 2 cut(s) 19, 477
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 4 cut(s) 31, 232, 265, 759
ClaI ATCGAT 2 cut(s) 77, 223
CseI GACGC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 575, 769
CviAII CATG 1 cut(s) 480
CviQI GTAC 2 cut(s) 575, 769
DdeI CTNAG 2 cut(s) 308, 563
DpnI GATC 3 cut(s) 76, 132, 226
DpnII GATC 3 cut(s) 74, 130, 224
Eam1104I CTCTTC 2 cut(s) 179, 701
EarI CTCTTC 2 cut(s) 179, 701
Ecl136II GAGCTC 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 31
Eco53kI GAGCTC 1 cut(s) 252
Eco88I CYCGRG 1 cut(s) 346
EcoICRI GAGCTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 544
EcoT22I ATGCAT 1 cut(s) 796
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 1 cut(s) 483
FaiI YATR 6 cut(s) 470, 481, 552, 554, 719, 774
FalI AAGNNNNNCTT 2 cut(s) 176, 208
FaqI GGGAC 1 cut(s) 794
FatI CATG 1 cut(s) 479
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 2 cut(s) 264, 327
FokI GGATG 1 cut(s) 8
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 2 cut(s) 264, 327
FspBI CTAG 2 cut(s) 488, 624
GlaI GCGC 1 cut(s) 519
GluI GCNGC 2 cut(s) 264, 327
GsaI CCCAGC 1 cut(s) 272
GsuI CTGGAG 1 cut(s) 696
HaeIII GGCC 5 cut(s) 140, 233, 266, 544, 761
HgaI GACGC 1 cut(s) 114
HhaI GCGC 1 cut(s) 520
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 1 cut(s) 483
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 4 cut(s) 398, 657, 678, 689
HphI GGTGA 1 cut(s) 327
Hpy166II GTNNAC 2 cut(s) 49, 240
Hpy188I TCNGA 6 cut(s) 26, 35, 74, 130, 418, 566
Hpy188III TCNNGA 9 cut(s) 295, 346, 395, 402, 442, 488, 604, 661, 799
Hpy8I GTNNAC 2 cut(s) 49, 240
Hpy99I CGWCG 2 cut(s) 130, 594
HpyAV CCTTC 2 cut(s) 30, 508
HpyCH4IV ACGT 3 cut(s) 537, 592, 628
HpyCH4V TGCA 7 cut(s) 4, 357, 362, 432, 475, 559, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 269
HpyF3I CTNAG 2 cut(s) 308, 563
HpySE526I ACGT 3 cut(s) 537, 592, 628
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 1 cut(s) 483
HspAI GCGC 1 cut(s) 518
Kzo9I GATC 3 cut(s) 74, 130, 224
LmnI GCTCC 2 cut(s) 83, 430
LweI GCATC 2 cut(s) 30, 441
MaeI CTAG 2 cut(s) 488, 624
MaeII ACGT 3 cut(s) 537, 592, 628
MalI GATC 3 cut(s) 76, 132, 226
MboI GATC 3 cut(s) 74, 130, 224
MboII GAAGA 5 cut(s) 171, 196, 219, 688, 757
MfeI CAATTG 1 cut(s) 92
MhlI GDGCHC 2 cut(s) 254, 304
MluCI AATT 8 cut(s) 60, 92, 152, 280, 373, 437, 651, 726
MlyI GAGTC 1 cut(s) 687
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 7 cut(s) 95, 151, 348, 438, 499, 657, 750
Mph1103I ATGCAT 1 cut(s) 796
MseI TTAA 2 cut(s) 11, 729
MspR9I CCNGG 1 cut(s) 546
MunI CAATTG 1 cut(s) 92
Mva1269I GAATGC 1 cut(s) 794
MvaI CCWGG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 269
NdeII GATC 3 cut(s) 74, 130, 224
NlaIII CATG 1 cut(s) 483
NlaIV GGNNCC 2 cut(s) 234, 267
NsiI ATGCAT 1 cut(s) 796
NspI RCATGY 1 cut(s) 483
NspV TTCGAA 1 cut(s) 182
PaqCI CACCTGC 1 cut(s) 342
PceI AGGCCT 1 cut(s) 544
PctI GAATGC 1 cut(s) 794
PfeI GAWTC 3 cut(s) 398, 657, 689
PkrI GCNGC 2 cut(s) 265, 328
PleI GAGTC 1 cut(s) 686
PpsI GAGTC 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 2 cut(s) 234, 267
PspPI GGNCC 4 cut(s) 31, 232, 265, 759
RsaI GTAC 2 cut(s) 576, 770
RsaNI GTAC 2 cut(s) 575, 769
SacI GAGCTC 1 cut(s) 254
SalI GTCGAC 1 cut(s) 47
SaqAI TTAA 2 cut(s) 11, 729
SatI GCNGC 2 cut(s) 264, 327
Sau3AI GATC 3 cut(s) 74, 130, 224
Sau96I GGNCC 4 cut(s) 31, 232, 265, 759
SchI GAGTC 1 cut(s) 687
ScrFI CCNGG 1 cut(s) 546
SduI GDGCHC 2 cut(s) 254, 304
SfaNI GCATC 2 cut(s) 30, 441
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 393
SmoI CTYRAG 1 cut(s) 393
Sse9I AATT 8 cut(s) 60, 92, 152, 280, 373, 437, 651, 726
SseBI AGGCCT 1 cut(s) 544
SsiI CCGC 3 cut(s) 263, 326, 754
SspMI CTAG 2 cut(s) 488, 624
SstI GAGCTC 1 cut(s) 254
StuI AGGCCT 1 cut(s) 544
StyD4I CCNGG 1 cut(s) 544
TaiI ACGT 3 cut(s) 540, 595, 631
TaqI TCGA 6 cut(s) 48, 77, 182, 223, 304, 319
TasI AATT 8 cut(s) 60, 92, 152, 280, 373, 437, 651, 726
TatI WGTACW 1 cut(s) 768
TauI GCSGC 2 cut(s) 266, 329
TfiI GAWTC 3 cut(s) 398, 657, 689
Tru1I TTAA 2 cut(s) 11, 729
Tru9I TTAA 2 cut(s) 11, 729
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 2 cut(s) 373, 437
XbaI TCTAGA 1 cut(s) 487
XceI RCATGY 1 cut(s) 483
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 488, 624
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.