Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0003249

Physical Location & Seq

Genomic Coordinates Forward (+)

34284006 .. 34288233

Gene Structure 4228 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1209500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGACAGACCAAAACCTTCACGCGCCGCAGGAGGACGAGACGAAGAAGAAGAAGCGCAAGAGAAACAGAGCGAAGAAACCGTCGGAATCGGCGGGTACGGAGCCCGAAAATACTAAGGGGGAACAGGCTAAGGGCGAGAGCGAGAAGAAGAGGAAGAAGGTGAAGAAATTGAAGGGAGAGGAACCGGAGGCTCAGAGCCCTGTGGAGGACGAAGAGGAAGAAGAGGAGGAGGGGGAAGAAGCAATGGAGGAGGAGGAAGAGGAGGAGAAGAAGAAGGTGAGGAGTGGCGGCGGAGGGACTGGGATTATGAGCACGGAGTCGTTTGCTTCGTTGAATTTGTCTGAGAACACTTACAAGGCCATTGAAGAAATGAAGTTTCAGTATATGACTCAGATTCAAGCTAGAGCAATCCCCCCGCTTTTGATCGGGAAAGATGTCCTCGGAGCTGCAAGGACAGGGTCTGGCAAAACTCTTGCTTTTCTAATACCAGCTGTGGAGCTGCTATATAACACACATTTTGCTCCTCGTAATGGAATGGGTGTTGTTGTGATTTGCCCAACACGGGAACTTGCGATACAGACCCATGCTGTGGCAAAGGACCTTCTAAAGTATCACTCTCAGACTCTTGGCTTGGTAATTGGAGGTTCAGCTAGGAGAGGGGAAGCAGAACGTATTGTAAAAGGAGTAAATTTATTGGTTGCAACCCCTGGTCGACTTCTTGACCATCTTCAGAATACCAAAGGATTCATATATAAGAACTTGAAGTGCCTTGTGATTGATGAAGCTGACAGGTTATTGGAAGCAAACTTTGAGGAGGAAATGAAACAGATTGTCAAGATTCTGCCAAAGGATAGGCAAACAGCTTTATTTTCAGCAACCCAAACTAAAAAGGTTGAAGATCTTGTTAAGTTGTCGTTTCGAAAAGATCAGAAACCTATCTACGTTGATGTGGATGAGGGGAGGTCAAAGGTCACCAATGAAGGGCTGGAACAAGGCTATTGTGTTGTGCCAAGTGCTAAGAGATTTCTACTCTTATATTCTTTCCTGACAAAGAATACGTCTAAGAAAGTTATGGTCTTCTTCTCGTCTTGTAACTCAGTGAAATTCCACTCCGAACTTCTTAGATACGTTAATGTGGATTGCTTTGATATCCATGGAAAGCAAAAGCAGCAGAAGAGGACAACTACTTTCTTTGACTTTTGCAAAGCAGAGAAAGGAATCTTACTTTGTACTGATGTTGCTGCTCGTGGACTAGATATTCCTGCTGTGGACTGGATTTTGCAGTTTGATCCTCCAGATGAACCGACGGAATATATCCATAGGGTTGGTCGAACAGCTCGAGGGGAAGGTGCAAAAGGAAAGGCACTACTTTTCCTCATTCCTGAAGAGTTGCAATTCATTCGCTATTTGAAGGCTGCAAATGTCCCCGTTAAACCCTACGATTTTAATGAAAAGAAGCTGAAGAATGTGCAGTCTAAACTGGAGGAGATGGTTCAAAACAATTACTACCTAAATAAATCAGCAAAAGAGGCGTATACATCTTATCTATTAGCATATAATTCACACTCTATGAAGGACATCTTTAATGTACACCGGCTTGATCTGCAGGCGGTTGCAGCTTCGTTTTGCTTTTCTAATCCACCCAAGGTGCATTTGAACCTAGACAGCAACAAATCAAAGTTCAAGAAAAAGACGCATAAAGAAGCAAGTCGAAATGGATTCAGCGAGAGCAATCCTTACGGCAATCGAAAGGGCGGAGACGATAGACAATTTGTAAGGCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

595

Amino Acids

67.19

Weight (kDa)

8.96

Isoelectric Point (pI)

42.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	130 - 301	1.8e-47	DEAD/DEAH box helicase
ResIII	PF04851	147 - 293	3.5e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	344 - 447	1.1e-24	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	501 - 550	5.5e-18	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18600
fragaria_vesca	FvH4_5g12870
malus_domestica	MD05G1177700.v1.1 MD06G1209500.v1.1 MD14G1220200.v1.1 MD15G1015500.v1.1
prunus_persica	Prupe.5G214700_v2.0.a1 Prupe.6G096100_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G097300_v2.0.a1
pyrus_communis	pycom05g16360 pycom05g16400 pycom05g16410 pycom06g18710 pycom14g18230 pycom15g01320
rosa_chinensis	RchiOBHm_Chr5g0083351 RchiOBHm_Chr5g0083361 RchiOBHm_Chr7g0184361
rosa_laevigata	RLG00000004982 RLG00000037030
rosa_multiflora	Rmu_sc0001444.1_g000013 Rmu_sc0001557.1_g000022 Rmu_sc0002245.1_g000045
rosa_roxburghii	Rroxscaffold_1G00001130 Rroxscaffold_3G00269690
rosa_samantha	Rh5AG533800 Rh5BG561600 Rh5CG583900 Rh5CG584000 Rh7AG080700 Rh7BG066500 Rh7CG066800 Rh7DG066500
rosa_wichuraiana	Rw5G049900 Rw7G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	589
AccI	GTMKAC	2 cut(s)	712, 1535
AccII	CGCG	1 cut(s)	23
AciI	CCGC	7 cut(s)	26, 92, 288, 291, 416, 1610, 1755
AclWI	GGATC	1 cut(s)	1283
AcsI	RAATTY	3 cut(s)	334, 688, 1103
AcuI	CTGAAG	3 cut(s)	713, 1404, 1481
AfaI	GTAC	3 cut(s)	97, 1231, 1590
AfiI	CCNNNNNNNGG	5 cut(s)	205, 530, 562, 589, 981
AjnI	CCWGG	1 cut(s)	706
Alw21I	GWGCWC	1 cut(s)	314
Alw26I	GTCTC	2 cut(s)	32, 1752
AlwI	GGATC	1 cut(s)	1283
AlwNI	CAGNNNCTG	1 cut(s)	461
Ama87I	CYCGRG	1 cut(s)	1338
AoxI	GGCC	1 cut(s)	357
ApeKI	GCWGC	6 cut(s)	446, 499, 1168, 1241, 1415, 1616
ApoI	RAATTY	3 cut(s)	334, 688, 1103
ArsI	GACNNNNNNTTYG	4 cut(s)	65, 97, 1043, 1075
AspLEI	GCGC	2 cut(s)	25, 57
AspS9I	GGNCC	1 cut(s)	598
AsuHPI	GGTGA	3 cut(s)	172, 289, 964
AsuII	TTCGAA	1 cut(s)	919
AvaI	CYCGRG	1 cut(s)	1338
AvaII	GGWCC	1 cut(s)	598
BanII	GRGCYC	2 cut(s)	105, 200
BauI	CACGAG	1 cut(s)	1245
BbsI	GAAGAC	1 cut(s)	1069
Bbv12I	GWGCWC	1 cut(s)	314
BbvI	GCAGC	6 cut(s)	433, 486, 1180, 1228, 1402, 1628
BccI	CCATC	2 cut(s)	732, 1483
BceAI	ACGGC	1 cut(s)	1756
BcgI	CGANNNNNNTGC	2 cut(s)	1382, 1416
BciT130I	CCWGG	1 cut(s)	708
BcoDI	GTCTC	2 cut(s)	32, 1752
BfaI	CTAG	5 cut(s)	402, 651, 1253, 1661, 1783
BfmI	CTRYAG	1 cut(s)	1604
BglII	AGATCT	1 cut(s)	898
BisI	GCNGC	8 cut(s)	26, 289, 447, 500, 1169, 1242, 1416, 1617
BlsI	GCNGC	8 cut(s)	27, 290, 448, 501, 1170, 1243, 1417, 1618
Bme1390I	CCNGG	1 cut(s)	708
Bme18I	GGWCC	1 cut(s)	598
BmeT110I	CYCGRG	1 cut(s)	1338
BmgT120I	GGNCC	1 cut(s)	598
BmiI	GGNNCC	2 cut(s)	102, 183
BmrFI	CCNGG	1 cut(s)	708
BmrI	ACTGGG	1 cut(s)	309
BmuI	ACTGGG	1 cut(s)	309
BpiI	GAAGAC	1 cut(s)	1069
BpmI	CTGGAG	2 cut(s)	1278, 1502
Bpu10I	CCTNAGC	1 cut(s)	129
Bpu14I	TTCGAA	1 cut(s)	919
BsaJI	CCNNGG	4 cut(s)	439, 706, 1153, 1644
BsaWI	WCCGGW	1 cut(s)	184
Bsc4I	CCNNNNNNNGG	5 cut(s)	205, 530, 562, 589, 981
Bse118I	RCCGGY	1 cut(s)	1593
Bse1I	ACTGG	3 cut(s)	304, 1277, 1485
Bse3DI	GCAATG	1 cut(s)	249
BseBI	CCWGG	1 cut(s)	708
BseDI	CCNNGG	4 cut(s)	439, 706, 1153, 1644
BseGI	GGATG	1 cut(s)	958
BseLI	CCNNNNNNNGG	5 cut(s)	205, 530, 562, 589, 981
BseMI	GCAATG	1 cut(s)	249
BseMII	CTCAG	5 cut(s)	206, 333, 404, 632, 1110
BseNI	ACTGG	3 cut(s)	304, 1277, 1485
BseXI	GCAGC	6 cut(s)	433, 486, 1180, 1228, 1402, 1628
BsgI	GTGCAG	1 cut(s)	1490
Bsh1236I	CGCG	1 cut(s)	23
BshFI	GGCC	1 cut(s)	359
BsiHKAI	GWGCWC	1 cut(s)	314
BsiHKCI	CYCGRG	1 cut(s)	1338
BsiSI	CCGG	2 cut(s)	185, 1594
BslFI	GGGAC	2 cut(s)	310, 1409
BslI	CCNNNNNNNGG	5 cut(s)	205, 530, 562, 589, 981
BsmAI	GTCTC	2 cut(s)	32, 1752
BsmBI	CGTCTC	2 cut(s)	32, 1752
BsmFI	GGGAC	2 cut(s)	310, 1409
BsnI	GGCC	1 cut(s)	359
BsoBI	CYCGRG	1 cut(s)	1338
Bsp119I	TTCGAA	1 cut(s)	919
Bsp1286I	GDGCHC	3 cut(s)	105, 200, 314
Bsp1407I	TGTACA	1 cut(s)	1588
Bsp143I	GATC	5 cut(s)	423, 898, 925, 1288, 1600
Bsp19I	CCATGG	1 cut(s)	1153
BspACI	CCGC	7 cut(s)	26, 92, 288, 291, 416, 1610, 1755
BspANI	GGCC	1 cut(s)	359
BspCNI	CTCAG	5 cut(s)	205, 334, 403, 631, 1109
BspFNI	CGCG	1 cut(s)	23
BspLI	GGNNCC	2 cut(s)	102, 183
BspMAI	CTGCAG	1 cut(s)	1608
BspPI	GGATC	1 cut(s)	1283
BspT104I	TTCGAA	1 cut(s)	919
BsrDI	GCAATG	1 cut(s)	249
BsrFI	RCCGGY	1 cut(s)	1593
BsrGI	TGTACA	1 cut(s)	1588
BsrI	ACTGG	3 cut(s)	304, 1277, 1485
BssAI	RCCGGY	1 cut(s)	1593
BssECI	CCNNGG	4 cut(s)	439, 706, 1153, 1644
BssMI	GATC	5 cut(s)	423, 898, 925, 1288, 1600
BssNAI	GTATAC	1 cut(s)	1536
BssSI	CACGAG	1 cut(s)	1245
BssT1I	CCWWGG	2 cut(s)	1153, 1644
Bst1107I	GTATAC	1 cut(s)	1536
Bst2BI	CACGAG	1 cut(s)	1245
Bst2UI	CCWGG	1 cut(s)	708
Bst4CI	ACNGT	1 cut(s)	81
Bst6I	CTCTTC	6 cut(s)	143, 207, 216, 252, 1169, 1380
BstAUI	TGTACA	1 cut(s)	1588
BstBI	TTCGAA	1 cut(s)	919
BstC8I	GCNNGC	1 cut(s)	1608
BstDSI	CCRYGG	1 cut(s)	1153
BstEII	GGTNACC	1 cut(s)	970
BstF5I	GGATG	1 cut(s)	958
BstFNI	CGCG	1 cut(s)	23
BstHHI	GCGC	2 cut(s)	25, 57
BstKTI	GATC	5 cut(s)	426, 901, 928, 1291, 1603
BstMAI	GTCTC	2 cut(s)	32, 1752
BstMBI	GATC	5 cut(s)	423, 898, 925, 1288, 1600
BstMWI	GCNNNNNNNGC	4 cut(s)	1168, 1529, 1603, 1616
BstNI	CCWGG	1 cut(s)	708
BstPI	GGTNACC	1 cut(s)	970
BstSCI	CCNGG	1 cut(s)	706
BstSFI	CTRYAG	1 cut(s)	1604
BstUI	CGCG	1 cut(s)	23
BstV1I	GCAGC	6 cut(s)	433, 486, 1180, 1228, 1402, 1628
BstV2I	GAAGAC	1 cut(s)	1069
BstX2I	RGATCY	1 cut(s)	898
BstXI	CCANNNNNNTGG	1 cut(s)	1325
BstYI	RGATCY	1 cut(s)	898
BstZ17I	GTATAC	1 cut(s)	1536
BsuRI	GGCC	1 cut(s)	359
BtgI	CCRYGG	1 cut(s)	1153
BtsCI	GGATG	1 cut(s)	958
BtsIMutI	CAGTG	1 cut(s)	1104
Cac8I	GCNNGC	1 cut(s)	1608
CaiI	CAGNNNCTG	1 cut(s)	461
CfoI	GCGC	2 cut(s)	25, 57
Cfr10I	RCCGGY	1 cut(s)	1593
Cfr13I	GGNCC	1 cut(s)	598
CseI	GACGC	1 cut(s)	1702
Csp6I	GTAC	3 cut(s)	96, 1230, 1589
CviAII	CATG	2 cut(s)	584, 1154
CviQI	GTAC	3 cut(s)	96, 1230, 1589
DpnI	GATC	5 cut(s)	425, 900, 927, 1290, 1602
DpnII	GATC	5 cut(s)	423, 898, 925, 1288, 1600
Eam1104I	CTCTTC	6 cut(s)	143, 207, 216, 252, 1169, 1380
EarI	CTCTTC	6 cut(s)	143, 207, 216, 252, 1169, 1380
EciI	GGCGGA	2 cut(s)	306, 1770
Eco130I	CCWWGG	2 cut(s)	1153, 1644
Eco24I	GRGCYC	2 cut(s)	105, 200
Eco32I	GATATC	1 cut(s)	1150
Eco47I	GGWCC	1 cut(s)	598
Eco57I	CTGAAG	3 cut(s)	713, 1404, 1481
Eco88I	CYCGRG	1 cut(s)	1338
Eco91I	GGTNACC	1 cut(s)	970
EcoO109I	RGGNCCY	1 cut(s)	598
EcoO65I	GGTNACC	1 cut(s)	970
EcoRII	CCWGG	1 cut(s)	706
EcoRV	GATATC	1 cut(s)	1150
EcoT14I	CCWWGG	2 cut(s)	1153, 1644
EcoT38I	GRGCYC	2 cut(s)	105, 200
ErhI	CCWWGG	2 cut(s)	1153, 1644
Esp3I	CGTCTC	2 cut(s)	32, 1752
FaeI	CATG	2 cut(s)	587, 1157
FalI	AAGNNNNNCTT	2 cut(s)	1565, 1597
FaqI	GGGAC	2 cut(s)	310, 1409
FatI	CATG	2 cut(s)	583, 1153
FauI	CCCGC	2 cut(s)	85, 423
FblI	GTMKAC	2 cut(s)	712, 1535
Fnu4HI	GCNGC	8 cut(s)	26, 289, 447, 500, 1169, 1242, 1416, 1617
FokI	GGATG	1 cut(s)	965
FriOI	GRGCYC	2 cut(s)	105, 200
Fsp4HI	GCNGC	8 cut(s)	26, 289, 447, 500, 1169, 1242, 1416, 1617
FspBI	CTAG	5 cut(s)	402, 651, 1253, 1661, 1783
GlaI	GCGC	2 cut(s)	24, 56
GluI	GCNGC	8 cut(s)	26, 289, 447, 500, 1169, 1242, 1416, 1617
GsuI	CTGGAG	2 cut(s)	1278, 1502
HaeIII	GGCC	1 cut(s)	359
HapII	CCGG	2 cut(s)	185, 1594
HgaI	GACGC	1 cut(s)	1702
HhaI	GCGC	2 cut(s)	25, 57
Hin1II	CATG	2 cut(s)	587, 1157
Hin6I	GCGC	2 cut(s)	23, 55
HinP1I	GCGC	2 cut(s)	23, 55
HincII	GTYRAC	1 cut(s)	713
HindII	GTYRAC	1 cut(s)	713
HinfI	GANTC	9 cut(s)	86, 317, 388, 394, 622, 744, 838, 1218, 1719
HpaII	CCGG	2 cut(s)	185, 1594
HphI	GGTGA	3 cut(s)	172, 289, 964
Hpy166II	GTNNAC	5 cut(s)	713, 1250, 1270, 1536, 1591
Hpy188I	TCNGA	9 cut(s)	85, 195, 343, 393, 443, 621, 732, 930, 1114
Hpy188III	TCNNGA	7 cut(s)	427, 719, 835, 1045, 1295, 1382, 1684
Hpy8I	GTNNAC	5 cut(s)	713, 1250, 1270, 1536, 1591
Hpy99I	CGWCG	2 cut(s)	85, 1309
HpyAV	CCTTC	9 cut(s)	26, 151, 166, 268, 611, 974, 1340, 1405, 1567
HpyCH4III	ACNGT	1 cut(s)	81
HpyCH4IV	ACGT	4 cut(s)	670, 942, 1058, 1128
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1168, 1529, 1603, 1616
HpySE526I	ACGT	4 cut(s)	670, 942, 1058, 1128
Hsp92II	CATG	2 cut(s)	587, 1157
HspAI	GCGC	2 cut(s)	23, 55
Kzo9I	GATC	5 cut(s)	423, 898, 925, 1288, 1600
LmnI	GCTCC	4 cut(s)	100, 443, 496, 526
Lsp1109I	GCAGC	6 cut(s)	433, 486, 1180, 1228, 1402, 1628
MaeI	CTAG	5 cut(s)	402, 651, 1253, 1661, 1783
MaeII	ACGT	4 cut(s)	670, 942, 1058, 1128
MaeIII	GTNAC	2 cut(s)	970, 1091
MalI	GATC	5 cut(s)	425, 900, 927, 1290, 1602
MboI	GATC	5 cut(s)	423, 898, 925, 1288, 1600
MflI	RGATCY	1 cut(s)	898
MhlI	GDGCHC	3 cut(s)	105, 200, 314
MluCI	AATT	9 cut(s)	167, 334, 636, 688, 1103, 1394, 1501, 1558, 1769
MlyI	GAGTC	3 cut(s)	326, 382, 616
MmeI	TCCRAC	1 cut(s)	63
MseI	TTAA	5 cut(s)	906, 1131, 1431, 1446, 1584
MspA1I	CMGCKG	1 cut(s)	491
MspI	CCGG	2 cut(s)	185, 1594
MspR9I	CCNGG	1 cut(s)	708
MvaI	CCWGG	1 cut(s)	708
MvnI	CGCG	1 cut(s)	23
MwoI	GCNNNNNNNGC	4 cut(s)	1168, 1529, 1603, 1616
NcoI	CCATGG	1 cut(s)	1153
NdeII	GATC	5 cut(s)	423, 898, 925, 1288, 1600
NlaIII	CATG	2 cut(s)	587, 1157
NlaIV	GGNNCC	2 cut(s)	102, 183
NmuCI	GTSAC	1 cut(s)	970
NspV	TTCGAA	1 cut(s)	919
PaeR7I	CTCGAG	1 cut(s)	1338
PcsI	WCGNNNNNNNCGW	2 cut(s)	326, 1336
PfeI	GAWTC	6 cut(s)	86, 394, 744, 838, 1218, 1719
PflFI	GACNNNGTC	1 cut(s)	457
PflMI	CCANNNNNTGG	1 cut(s)	589
PkrI	GCNGC	8 cut(s)	27, 290, 448, 501, 1170, 1243, 1417, 1618
PleI	GAGTC	3 cut(s)	325, 382, 616
PpsI	GAGTC	3 cut(s)	325, 382, 616
PpuMI	RGGWCCY	1 cut(s)	598
Psp5II	RGGWCCY	1 cut(s)	598
Psp6I	CCWGG	1 cut(s)	706
PspEI	GGTNACC	1 cut(s)	970
PspGI	CCWGG	1 cut(s)	706
PspN4I	GGNNCC	2 cut(s)	102, 183
PspPI	GGNCC	1 cut(s)	598
PspPPI	RGGWCCY	1 cut(s)	598
PspXI	VCTCGAGB	1 cut(s)	1338
PsrI	GAACNNNNNNTAC	2 cut(s)	558, 590
PstI	CTGCAG	1 cut(s)	1608
PstNI	CAGNNNCTG	1 cut(s)	461
PsuI	RGATCY	1 cut(s)	898
PsyI	GACNNNGTC	1 cut(s)	457
PvuII	CAGCTG	1 cut(s)	491
RsaI	GTAC	3 cut(s)	97, 1231, 1590
RsaNI	GTAC	3 cut(s)	96, 1230, 1589
SalI	GTCGAC	1 cut(s)	711
SaqAI	TTAA	5 cut(s)	906, 1131, 1431, 1446, 1584
SatI	GCNGC	8 cut(s)	26, 289, 447, 500, 1169, 1242, 1416, 1617
Sau3AI	GATC	5 cut(s)	423, 898, 925, 1288, 1600
Sau96I	GGNCC	1 cut(s)	598
SchI	GAGTC	3 cut(s)	326, 382, 616
ScrFI	CCNGG	1 cut(s)	708
SduI	GDGCHC	3 cut(s)	105, 200, 314
SfcI	CTRYAG	1 cut(s)	1604
Sfr274I	CTCGAG	1 cut(s)	1338
SfuI	TTCGAA	1 cut(s)	919
SinI	GGWCC	1 cut(s)	598
SlaI	CTCGAG	1 cut(s)	1338
SmlI	CTYRAG	1 cut(s)	1338
SmoI	CTYRAG	1 cut(s)	1338
Sse9I	AATT	9 cut(s)	167, 334, 636, 688, 1103, 1394, 1501, 1558, 1769
SsiI	CCGC	7 cut(s)	26, 92, 288, 291, 416, 1610, 1755
SspMI	CTAG	5 cut(s)	402, 651, 1253, 1661, 1783
StyD4I	CCNGG	1 cut(s)	706
StyI	CCWWGG	2 cut(s)	1153, 1644
TaaI	ACNGT	1 cut(s)	81
TaiI	ACGT	4 cut(s)	673, 945, 1061, 1131
TaqI	TCGA	6 cut(s)	712, 919, 1330, 1339, 1711, 1747
TasI	AATT	9 cut(s)	167, 334, 636, 688, 1103, 1394, 1501, 1558, 1769
TatI	WGTACW	2 cut(s)	1229, 1588
TauI	GCSGC	2 cut(s)	28, 291
TfiI	GAWTC	6 cut(s)	86, 394, 744, 838, 1218, 1719
Tru1I	TTAA	5 cut(s)	906, 1131, 1431, 1446, 1584
Tru9I	TTAA	5 cut(s)	906, 1131, 1431, 1446, 1584
TscAI	CASTG	1 cut(s)	1104
TseFI	GTSAC	1 cut(s)	970
TseI	GCWGC	6 cut(s)	446, 499, 1168, 1241, 1415, 1616
Tsp45I	GTSAC	1 cut(s)	970
TspDTI	ATGAA	9 cut(s)	386, 736, 795, 836, 993, 1314, 1387, 1464, 1586
TspGWI	ACGGA	3 cut(s)	113, 329, 1322
TspRI	CASTG	1 cut(s)	1104
Tth111I	GACNNNGTC	1 cut(s)	457
Van91I	CCANNNNNTGG	1 cut(s)	589
VpaK11BI	GGWCC	1 cut(s)	598
XapI	RAATTY	3 cut(s)	334, 688, 1103
XcmI	CCANNNNNNNNNTGG	1 cut(s)	982
XhoI	CTCGAG	1 cut(s)	1338
XmiI	GTMKAC	2 cut(s)	712, 1535
XspI	CTAG	5 cut(s)	402, 651, 1253, 1661, 1783

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.