Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003249

Physical Location & Seq

Genomic Coordinates Reverse (-)

64152594 .. 64156638

Gene Structure 4045 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004982

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGACCGACCCAATCTCCCACGCATCTCCCATGGCTGACGCGAAGAAGAGGAAGCGCAAGAGAAACAGAGCGAAGAAGCCATCGGAATCGGAAGTTGCAGAGCAACCGGAGCCTCAAATTGAAGAAGAAGCGGAAAACGGAGAAGGCGAGGAGCAAGCTAAGAAGAAGGAGAAGAAGAGGAAGAAGATGAAGAAATTGAAGGGAGAGGAGGAAGCAGCAGAGGCTGAAATCCCTAGGGAGACCAAAGAAGAAGAAGAAGATGAAGATGCAGAAGAGGAAGAGGAGAAGGAAGAGAAGGAAGAGAAGAAAGAGAAGAAGGTGAGGAGTGGTGGAGGGACTGGGATTATGAGCACAGTGACCTTTGCTTCATTGAATTTATCTGAGAAAACTTTCTCGGCCATTCAAGAATTGGGCTTTCAGCATATGACTCAGATTCAATCCAGAGCAATCCCACCGCTGTTGATCGGAAGAGATGTGCTTGGAGCTGCAAGGACAGGGTCCGGGAAGACTCTTGCTTTTCTGGTGCCAGCTGTGGAGTTGTTATATAACGCAAGCTTTTCACCTCGTAATGGAACTGGTGTTGTTGTCATTTGCCCAACTAGGGAACTCGCAATACAGTCCCATGCCGTGGCCAAGGACCTTCTCAAGAATCACTCGCAGACTCTTGGTTTGGTTATTGGTGGTTCGGCTAGAAGAGGAGAAGCAGAGCGTCTTGCAAAAGGAGTAAACCTATTGGTTGCAACTCCTGGTCGCCTTCTTGACCATCTGCAGAACACCAAGGGATTTGTATATAAGAACTTGAAGTGCCTTATCATTGATGAAGCTGACAGGATACTGGAAGCAAACTTTGAGGAAGAAATGAAACAAATCATTAACATTCTACCAAAGAATAGGCAAACAGCTTTATTTTCTGCAACCCAGACTAAGAAGGTTGAAGATCTTGCTCGCTTGTCCTTCCAGACAACGCCTATGTATATAGATGTGGATGATGGGAGAACTAAGGTCACGAATGAAGGGCTGCAACAAGGCTATTGTCTTGTGCCAAGTGCCAAGAGATTTATTCTCCTATATTCTTTCCTGACGAAGAATCTATCAAAGAAAGTGATGGTCTTCTTCTCTTCTTGTAACTCAGTGAAATTCCACTCCGACCTTCTTAGATACATTAATGTGGATTGCTTTGATATCCATGGAAAGCAAAAGCAGCAGAAGAGGACAAGTACTTTTTTTGACTACTGCAAAGCAGAGAAAGGAATCTTACTTTGTACTGATGTTGCTGCTCGTGGCTTGGATATCCCTGCCGTGGACTGGATTGTGCAGTTTGATCCTCCAGATGAACCCAAGGAATATATCCACAGGGTTGGTCGAACAGCTCGTGGGGAAGGTGGAAAAGGAAATGCACTACTTTTCCTCATTCCTGAAGAGTTGCAGTTTCTTTGCTATTTGAGGGAAGCAAAAGTCCCTGTTAAAGAGTATGAATTTAATGAGAAGAAGCTAAAAAATGTGCAATCTCAGCTGGAGAAGTTGGTTGCCAACAACTACCATCTGAACCGTGCAGCAAAAGATGCATATAGGTCTTATCTATTAGCATATAATTCACACTCTATGAAGGACATCTTTAATGTTCACCGACTCGATCTCCAGGCAGTTGCTTCTTCATTTTGCTTTTCTAATCCACCGAAGGTAAATTTAAACTTAGACAGCAGTGCATCAAAGTTCCGGAAGAACATGCGCAAAGTGGATGGAGTTCGACATGGAATCAATCCTAACAATCCTTATGGAAGGCAAAGAGGTGGAGATGATGACATGAGACAATTTGTGAGACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

609

Amino Acids

68.66

Weight (kDa)

8.93

Isoelectric Point (pI)

43.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	143 - 314	6.9e-47	DEAD/DEAH box helicase
Helicase_C	PF00271	355 - 458	1.6e-23	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	512 - 561	3.6e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18600
fragaria_vesca	FvH4_5g12870
malus_domestica	MD05G1177700.v1.1 MD06G1209500.v1.1 MD14G1220200.v1.1 MD15G1015500.v1.1
prunus_persica	Prupe.5G214700_v2.0.a1 Prupe.6G096100_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G097300_v2.0.a1
pyrus_communis	pycom05g16360 pycom05g16400 pycom05g16410 pycom06g18710 pycom14g18230 pycom15g01320
rosa_chinensis	RchiOBHm_Chr5g0083351 RchiOBHm_Chr5g0083361 RchiOBHm_Chr7g0184361
rosa_laevigata	RLG00000004982 RLG00000037030
rosa_multiflora	Rmu_sc0001444.1_g000013 Rmu_sc0001557.1_g000022 Rmu_sc0002245.1_g000045
rosa_roxburghii	Rroxscaffold_1G00001130 Rroxscaffold_3G00269690
rosa_samantha	Rh5AG533800 Rh5BG561600 Rh5CG583900 Rh5CG584000 Rh7AG080700 Rh7BG066500 Rh7CG066800 Rh7DG066500
rosa_wichuraiana	Rw5G049900 Rw7G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1730
AccB1I	GGYRCC	1 cut(s)	523
AccB7I	CCANNNNNTGG	1 cut(s)	628
AccII	CGCG	1 cut(s)	41
AccIII	TCCGGA	1 cut(s)	1716
AciI	CCGC	2 cut(s)	131, 455
AclWI	GGATC	1 cut(s)	1316
AcoI	YGGCCR	2 cut(s)	396, 630
AcsI	RAATTY	4 cut(s)	373, 1136, 1475, 1684
AcuI	CTGAAG	1 cut(s)	1437
AfaI	GTAC	2 cut(s)	1219, 1264
AfiI	CCNNNNNNNGG	5 cut(s)	569, 601, 628, 1300, 1305
AgsI	TTSAA	7 cut(s)	122, 199, 373, 404, 437, 802, 935
AjnI	CCWGG	2 cut(s)	745, 1638
AluBI	AGCT	9 cut(s)	158, 485, 530, 555, 824, 902, 1370, 1492, 1513
AluI	AGCT	9 cut(s)	158, 485, 530, 555, 824, 902, 1370, 1492, 1513
Alw21I	GWGCWC	1 cut(s)	353
Alw26I	GTCTC	3 cut(s)	233, 1801, 1813
AlwI	GGATC	1 cut(s)	1316
AlwNI	CAGNNNCTG	1 cut(s)	224
Aor13HI	TCCGGA	1 cut(s)	1716
AoxI	GGCC	2 cut(s)	396, 630
ApeKI	GCWGC	6 cut(s)	215, 485, 1018, 1201, 1274, 1553
ApoI	RAATTY	4 cut(s)	373, 1136, 1475, 1684
ArsI	GACNNNNNNTTYG	2 cut(s)	652, 684
AseI	ATTAAT	1 cut(s)	1164
Asp700I	GAANNNNTTC	1 cut(s)	389
AspA2I	CCTAGG	1 cut(s)	233
AspLEI	GCGC	2 cut(s)	57, 1731
AspS9I	GGNCC	2 cut(s)	498, 637
AsuC2I	CCSGG	1 cut(s)	502
AsuHPI	GGTGA	3 cut(s)	331, 552, 1616
AvaII	GGWCC	2 cut(s)	498, 637
AvrII	CCTAGG	1 cut(s)	233
BalI	TGGCCA	1 cut(s)	632
BanI	GGYRCC	1 cut(s)	523
BauI	CACGAG	2 cut(s)	1278, 1371
BbsI	GAAGAC	2 cut(s)	512, 1102
Bbv12I	GWGCWC	1 cut(s)	353
BbvI	GCAGC	6 cut(s)	227, 472, 1005, 1213, 1261, 1565
BccI	CCATC	6 cut(s)	88, 771, 983, 1099, 1548, 1733
BceAI	ACGGC	2 cut(s)	611, 1283
BciT130I	CCWGG	2 cut(s)	747, 1640
BciVI	GTATCC	1 cut(s)	825
BcnI	CCSGG	1 cut(s)	502
BcoDI	GTCTC	3 cut(s)	233, 1801, 1813
BfaI	CTAG	3 cut(s)	234, 600, 690
BfmI	CTRYAG	1 cut(s)	767
BfuI	GTATCC	1 cut(s)	825
BglII	AGATCT	1 cut(s)	937
BisI	GCNGC	6 cut(s)	216, 486, 1019, 1202, 1275, 1554
BlnI	CCTAGG	1 cut(s)	233
BlsI	GCNGC	6 cut(s)	217, 487, 1020, 1203, 1276, 1555
BmcAI	AGTACT	1 cut(s)	1219
Bme1390I	CCNGG	3 cut(s)	502, 747, 1640
Bme18I	GGWCC	2 cut(s)	498, 637
BmgT120I	GGNCC	2 cut(s)	498, 637
BmiI	GGNNCC	3 cut(s)	111, 499, 525
BmrFI	CCNGG	3 cut(s)	502, 747, 1640
BmrI	ACTGGG	1 cut(s)	348
BmsI	GCATC	4 cut(s)	32, 256, 1552, 1715
BmuI	ACTGGG	1 cut(s)	348
BpiI	GAAGAC	2 cut(s)	512, 1102
BpmI	CTGGAG	3 cut(s)	1311, 1535, 1622
BpuEI	CTTGAG	1 cut(s)	629
BpuMI	CCSGG	1 cut(s)	502
BsaI	GGTCTC	1 cut(s)	233
BsaJI	CCNNGG	8 cut(s)	30, 233, 627, 633, 777, 1186, 1299, 1338
BsaWI	WCCGGW	2 cut(s)	106, 1716
BsaXI	ACNNNNNCTCC	3 cut(s)	29, 1620, 1650
Bsc4I	CCNNNNNNNGG	5 cut(s)	569, 601, 628, 1300, 1305
Bse1I	ACTGG	4 cut(s)	343, 580, 840, 1310
BseAI	TCCGGA	1 cut(s)	1716
BseBI	CCWGG	2 cut(s)	747, 1640
BseDI	CCNNGG	8 cut(s)	30, 233, 627, 633, 777, 1186, 1299, 1338
BseGI	GGATG	2 cut(s)	991, 1744
BseLI	CCNNNNNNNGG	5 cut(s)	569, 601, 628, 1300, 1305
BseMII	CTCAG	4 cut(s)	372, 443, 1143, 1523
BseNI	ACTGG	4 cut(s)	343, 580, 840, 1310
BseRI	GAGGAG	5 cut(s)	164, 221, 296, 337, 711
BseXI	GCAGC	6 cut(s)	227, 472, 1005, 1213, 1261, 1565
BsgI	GTGCAG	2 cut(s)	1334, 1572
Bsh1236I	CGCG	1 cut(s)	41
BshFI	GGCC	2 cut(s)	398, 632
BshNI	GGYRCC	1 cut(s)	523
BsiHKAI	GWGCWC	1 cut(s)	353
BsiSI	CCGG	3 cut(s)	107, 501, 1717
BslFI	GGGAC	3 cut(s)	349, 604, 1442
BslI	CCNNNNNNNGG	5 cut(s)	569, 601, 628, 1300, 1305
BsmAI	GTCTC	3 cut(s)	233, 1801, 1813
BsmFI	GGGAC	3 cut(s)	349, 604, 1442
BsnI	GGCC	2 cut(s)	398, 632
Bso31I	GGTCTC	1 cut(s)	233
Bsp1286I	GDGCHC	1 cut(s)	353
Bsp13I	TCCGGA	1 cut(s)	1716
Bsp143I	GATC	4 cut(s)	462, 937, 1321, 1633
Bsp19I	CCATGG	2 cut(s)	30, 1186
BspACI	CCGC	2 cut(s)	131, 455
BspANI	GGCC	2 cut(s)	398, 632
BspCNI	CTCAG	4 cut(s)	373, 442, 1142, 1522
BspEI	TCCGGA	1 cut(s)	1716
BspFNI	CGCG	1 cut(s)	41
BspLI	GGNNCC	3 cut(s)	111, 499, 525
BspMAI	CTGCAG	1 cut(s)	771
BspPI	GGATC	1 cut(s)	1316
BspT107I	GGYRCC	1 cut(s)	523
BspTNI	GGTCTC	1 cut(s)	233
BsrI	ACTGG	4 cut(s)	343, 580, 840, 1310
BssECI	CCNNGG	8 cut(s)	30, 233, 627, 633, 777, 1186, 1299, 1338
BssMI	GATC	4 cut(s)	462, 937, 1321, 1633
BssSI	CACGAG	2 cut(s)	1278, 1371
BssT1I	CCWWGG	6 cut(s)	30, 233, 633, 777, 1186, 1338
Bst2BI	CACGAG	2 cut(s)	1278, 1371
Bst2UI	CCWGG	2 cut(s)	747, 1640
Bst4CI	ACNGT	3 cut(s)	355, 618, 1550
BstAPI	GCANNNNNTGC	1 cut(s)	1562
BstC8I	GCNNGC	4 cut(s)	156, 528, 553, 946
BstDEI	CTNAG	9 cut(s)	159, 381, 429, 924, 999, 1129, 1154, 1509, 1693
BstDSI	CCRYGG	4 cut(s)	30, 627, 1186, 1299
BstF5I	GGATG	2 cut(s)	991, 1744
BstFNI	CGCG	1 cut(s)	41
BstHHI	GCGC	2 cut(s)	57, 1731
BstKTI	GATC	4 cut(s)	465, 940, 1324, 1636
BstMAI	GTCTC	3 cut(s)	233, 1801, 1813
BstMBI	GATC	4 cut(s)	462, 937, 1321, 1633
BstMWI	GCNNNNNNNGC	5 cut(s)	109, 221, 1201, 1510, 1562
BstNI	CCWGG	2 cut(s)	747, 1640
BstNSI	RCATGY	1 cut(s)	1729
BstSCI	CCNGG	3 cut(s)	500, 745, 1638
BstSFI	CTRYAG	1 cut(s)	767
BstUI	CGCG	1 cut(s)	41
BstV1I	GCAGC	6 cut(s)	227, 472, 1005, 1213, 1261, 1565
BstV2I	GAAGAC	2 cut(s)	512, 1102
BstX2I	RGATCY	1 cut(s)	937
BstXI	CCANNNNNNTGG	1 cut(s)	1358
BstYI	RGATCY	1 cut(s)	937
BsuI	GTATCC	1 cut(s)	825
BsuRI	GGCC	2 cut(s)	398, 632
BtgI	CCRYGG	4 cut(s)	30, 627, 1186, 1299
BtsCI	GGATG	2 cut(s)	991, 1744
BtsI	GCAGTG	1 cut(s)	1708
BtsIMutI	CAGTG	3 cut(s)	360, 1137, 1708
Cac8I	GCNNGC	4 cut(s)	156, 528, 553, 946
CaiI	CAGNNNCTG	1 cut(s)	224
CfoI	GCGC	2 cut(s)	57, 1731
Cfr13I	GGNCC	2 cut(s)	498, 637
CseI	GACGC	2 cut(s)	47, 698
Csp6I	GTAC	2 cut(s)	1218, 1263
CspCI	CAANNNNNGTGG	2 cut(s)	441, 476
CviAII	CATG	6 cut(s)	31, 623, 1187, 1726, 1751, 1804
CviQI	GTAC	2 cut(s)	1218, 1263
DdeI	CTNAG	9 cut(s)	159, 381, 429, 924, 999, 1129, 1154, 1509, 1693
DpnI	GATC	4 cut(s)	464, 939, 1323, 1635
DpnII	GATC	4 cut(s)	462, 937, 1321, 1633
DraI	TTTAAA	1 cut(s)	1689
EaeI	YGGCCR	2 cut(s)	396, 630
Eco130I	CCWWGG	6 cut(s)	30, 233, 633, 777, 1186, 1338
Eco31I	GGTCTC	1 cut(s)	233
Eco32I	GATATC	2 cut(s)	1183, 1291
Eco47I	GGWCC	2 cut(s)	498, 637
Eco57I	CTGAAG	1 cut(s)	1437
EcoO109I	RGGNCCY	1 cut(s)	637
EcoRII	CCWGG	2 cut(s)	745, 1638
EcoRV	GATATC	2 cut(s)	1183, 1291
EcoT14I	CCWWGG	6 cut(s)	30, 233, 633, 777, 1186, 1338
EcoT22I	ATGCAT	1 cut(s)	1567
ErhI	CCWWGG	6 cut(s)	30, 233, 633, 777, 1186, 1338
FaeI	CATG	6 cut(s)	34, 626, 1190, 1729, 1754, 1807
FalI	AAGNNNNNCTT	2 cut(s)	1598, 1630
FaqI	GGGAC	3 cut(s)	349, 604, 1442
FatI	CATG	6 cut(s)	30, 622, 1186, 1725, 1750, 1803
FauNDI	CATATG	1 cut(s)	423
Fnu4HI	GCNGC	6 cut(s)	216, 486, 1019, 1202, 1275, 1554
FokI	GGATG	2 cut(s)	998, 1751
Fsp4HI	GCNGC	6 cut(s)	216, 486, 1019, 1202, 1275, 1554
FspBI	CTAG	3 cut(s)	234, 600, 690
FspI	TGCGCA	1 cut(s)	1730
GlaI	GCGC	2 cut(s)	56, 1730
GluI	GCNGC	6 cut(s)	216, 486, 1019, 1202, 1275, 1554
GsuI	CTGGAG	3 cut(s)	1311, 1535, 1622
HaeIII	GGCC	2 cut(s)	398, 632
HapII	CCGG	3 cut(s)	107, 501, 1717
HgaI	GACGC	2 cut(s)	47, 698
HhaI	GCGC	2 cut(s)	57, 1731
Hin1II	CATG	6 cut(s)	34, 626, 1190, 1729, 1754, 1807
Hin6I	GCGC	2 cut(s)	55, 1729
HinP1I	GCGC	2 cut(s)	55, 1729
HindIII	AAGCTT	1 cut(s)	553
HpaII	CCGG	3 cut(s)	107, 501, 1717
HphI	GGTGA	3 cut(s)	331, 552, 1616
Hpy166II	GTNNAC	3 cut(s)	727, 1303, 1624
Hpy188I	TCNGA	7 cut(s)	85, 91, 382, 432, 467, 1147, 1545
Hpy8I	GTNNAC	3 cut(s)	727, 1303, 1624
HpyCH4III	ACNGT	3 cut(s)	355, 618, 1550
HpyF10VI	GCNNNNNNNGC	5 cut(s)	109, 221, 1201, 1510, 1562
HpyF3I	CTNAG	9 cut(s)	159, 381, 429, 924, 999, 1129, 1154, 1509, 1693
Hsp92II	CATG	6 cut(s)	34, 626, 1190, 1729, 1754, 1807
HspAI	GCGC	2 cut(s)	55, 1729
Kpn2I	TCCGGA	1 cut(s)	1716
Kzo9I	GATC	4 cut(s)	462, 937, 1321, 1633
LmnI	GCTCC	3 cut(s)	109, 151, 482
Lsp1109I	GCAGC	6 cut(s)	227, 472, 1005, 1213, 1261, 1565
LweI	GCATC	4 cut(s)	32, 256, 1552, 1715
MaeI	CTAG	3 cut(s)	234, 600, 690
MaeIII	GTNAC	3 cut(s)	355, 1003, 1124
MalI	GATC	4 cut(s)	464, 939, 1323, 1635
MboI	GATC	4 cut(s)	462, 937, 1321, 1633
MflI	RGATCY	1 cut(s)	937
MhlI	GDGCHC	1 cut(s)	353
MlsI	TGGCCA	1 cut(s)	632
MluCI	AATT	9 cut(s)	117, 194, 373, 407, 1136, 1475, 1591, 1684, 1811
MluNI	TGGCCA	1 cut(s)	632
MlyI	GAGTC	4 cut(s)	421, 502, 655, 1623
MmeI	TCCRAC	1 cut(s)	1170
Mox20I	TGGCCA	1 cut(s)	632
Mph1103I	ATGCAT	1 cut(s)	1567
MroI	TCCGGA	1 cut(s)	1716
MroXI	GAANNNNTTC	1 cut(s)	389
MscI	TGGCCA	1 cut(s)	632
MseI	TTAA	6 cut(s)	873, 1164, 1464, 1479, 1617, 1688
MslI	CAYNNNNRTG	1 cut(s)	1166
Msp20I	TGGCCA	1 cut(s)	632
MspA1I	CMGCKG	3 cut(s)	457, 530, 1513
MspI	CCGG	3 cut(s)	107, 501, 1717
MspR9I	CCNGG	3 cut(s)	502, 747, 1640
MvaI	CCWGG	2 cut(s)	747, 1640
MvnI	CGCG	1 cut(s)	41
MwoI	GCNNNNNNNGC	5 cut(s)	109, 221, 1201, 1510, 1562
NciI	CCSGG	1 cut(s)	502
NcoI	CCATGG	2 cut(s)	30, 1186
NdeI	CATATG	1 cut(s)	423
NdeII	GATC	4 cut(s)	462, 937, 1321, 1633
NlaIII	CATG	6 cut(s)	34, 626, 1190, 1729, 1754, 1807
NlaIV	GGNNCC	3 cut(s)	111, 499, 525
NmeAIII	GCCGAG	1 cut(s)	374
NmuCI	GTSAC	2 cut(s)	355, 1003
NsbI	TGCGCA	1 cut(s)	1730
NsiI	ATGCAT	1 cut(s)	1567
NspI	RCATGY	1 cut(s)	1729
PcsI	WCGNNNNNNNCGW	2 cut(s)	144, 1369
PdmI	GAANNNNTTC	1 cut(s)	389
PfeI	GAWTC	6 cut(s)	86, 433, 649, 1087, 1251, 1755
PflFI	GACNNNGTC	1 cut(s)	496
PflMI	CCANNNNNTGG	1 cut(s)	628
PfoI	TCCNGGA	1 cut(s)	500
PkrI	GCNGC	6 cut(s)	217, 487, 1020, 1203, 1276, 1555
PleI	GAGTC	4 cut(s)	421, 502, 655, 1623
PpsI	GAGTC	4 cut(s)	421, 502, 655, 1623
PpuMI	RGGWCCY	1 cut(s)	637
PshBI	ATTAAT	1 cut(s)	1164
Psp5II	RGGWCCY	1 cut(s)	637
Psp6I	CCWGG	2 cut(s)	745, 1638
PspGI	CCWGG	2 cut(s)	745, 1638
PspN4I	GGNNCC	3 cut(s)	111, 499, 525
PspPI	GGNCC	2 cut(s)	498, 637
PspPPI	RGGWCCY	1 cut(s)	637
PsrI	GAACNNNNNNTAC	2 cut(s)	597, 629
PstI	CTGCAG	1 cut(s)	771
PstNI	CAGNNNCTG	1 cut(s)	224
PsuI	RGATCY	1 cut(s)	937
PsyI	GACNNNGTC	1 cut(s)	496
PvuII	CAGCTG	2 cut(s)	530, 1513
RsaI	GTAC	2 cut(s)	1219, 1264
RsaNI	GTAC	2 cut(s)	1218, 1263
RseI	CAYNNNNRTG	1 cut(s)	1166
SaqAI	TTAA	6 cut(s)	873, 1164, 1464, 1479, 1617, 1688
SatI	GCNGC	6 cut(s)	216, 486, 1019, 1202, 1275, 1554
Sau3AI	GATC	4 cut(s)	462, 937, 1321, 1633
Sau96I	GGNCC	2 cut(s)	498, 637
ScaI	AGTACT	1 cut(s)	1219
SchI	GAGTC	4 cut(s)	421, 502, 655, 1623
ScrFI	CCNGG	3 cut(s)	502, 747, 1640
SduI	GDGCHC	1 cut(s)	353
SfaNI	GCATC	4 cut(s)	32, 256, 1552, 1715
SfcI	CTRYAG	1 cut(s)	767
SinI	GGWCC	2 cut(s)	498, 637
SmiMI	CAYNNNNRTG	1 cut(s)	1166
SmlI	CTYRAG	1 cut(s)	644
SmoI	CTYRAG	1 cut(s)	644
Sse9I	AATT	9 cut(s)	117, 194, 373, 407, 1136, 1475, 1591, 1684, 1811
SsiI	CCGC	2 cut(s)	131, 455
SspMI	CTAG	3 cut(s)	234, 600, 690
StyD4I	CCNGG	3 cut(s)	500, 745, 1638
StyI	CCWWGG	6 cut(s)	30, 233, 633, 777, 1186, 1338
TaaI	ACNGT	3 cut(s)	355, 618, 1550
TaqI	TCGA	3 cut(s)	1363, 1632, 1747
TaqII	GACCGA	1 cut(s)	20
TasI	AATT	9 cut(s)	117, 194, 373, 407, 1136, 1475, 1591, 1684, 1811
TatI	WGTACW	2 cut(s)	1217, 1262
TfiI	GAWTC	6 cut(s)	86, 433, 649, 1087, 1251, 1755
Tru1I	TTAA	6 cut(s)	873, 1164, 1464, 1479, 1617, 1688
Tru9I	TTAA	6 cut(s)	873, 1164, 1464, 1479, 1617, 1688
TscAI	CASTG	3 cut(s)	360, 1137, 1708
TseFI	GTSAC	2 cut(s)	355, 1003
TseI	GCWGC	6 cut(s)	215, 485, 1018, 1201, 1274, 1553
Tsp45I	GTSAC	2 cut(s)	355, 1003
TspGWI	ACGGA	1 cut(s)	153
TspRI	CASTG	3 cut(s)	360, 1137, 1708
Tth111I	GACNNNGTC	1 cut(s)	496
Van91I	CCANNNNNTGG	1 cut(s)	628
VpaK11BI	GGWCC	2 cut(s)	498, 637
VspI	ATTAAT	1 cut(s)	1164
XapI	RAATTY	4 cut(s)	373, 1136, 1475, 1684
XceI	RCATGY	1 cut(s)	1729
XcmI	CCANNNNNNNNNTGG	1 cut(s)	406
XmaJI	CCTAGG	1 cut(s)	233
XmnI	GAANNNNTTC	1 cut(s)	389
XspI	CTAG	3 cut(s)	234, 600, 690
ZrmI	AGTACT	1 cut(s)	1219
Zsp2I	ATGCAT	1 cut(s)	1567

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.