Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0003249

Physical Location & Seq

Genomic Coordinates Forward (+)

4415950 .. 4419563

Gene Structure 3614 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG066500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1395 bp

ATGACTCAGATTCAATCCAGAGCAATCCCACCGCTGTTGATCGGAAGAGATGTGCTTGGAGCTGCAAGGACAGGGTCCGGGAAGACTCTTGCTTTTCTGGTGCCAGCTGTGGAGTTGTTATATAACGCAAGCTTTTCGCCTCGTAATGGAACTGGTGTTGTTGTCATTTGCCCAACTAGGGAACTTGCAATACAGTCCCATGCCGTGGCCAAGGACCTTCTCAAGAATCACTCGCAGACTCTTGGTTTGGTTATTGGTGGTTCGGCTAGAAGAGGAGAAGCAGAGCGTCTTGCAAAAGGAGTAAACCTATTGGTTGCAACTCCTGGTCGCCTTCTTGACCATCTGCAGAACACCAAGGGATTCGTATATAAGAACTTGAAGTGCCTCATCATTGATGAAGCTGACAGGATACTGGAAGCAAACTTTGAGGAAGAAATGAAACAAATCATTAACATTCTACCAAAGATAAATAATTTGAACAAACTGGGTAGTTGTGTAGTTGAAGATCTTGCTCGCTTGTCCTTCCAGACAACGCCTATGTATATAGATGTGGATGATGGGAGAACTAAGGTCACGAATGAAGGGCTGCAACAAGGCTATTGTCTTGTGCCAAGTGCCAAGAGATTTATTCTCCTATATTCCTTCCTGACGAAGAATCTATCAAAGAAAGTGATGGTCTTCTTCTCTTCTTGTAACTCAGTGAAATTCCACTCCGACCTTCTTAGATACATTAATGTGGATTGCTTTGATATCCATGGAAAGCAAAAGCAGCAGAAGAGGACAAGTACTTTTTTTGACTACTGCAAAGCAGAGAAAGGAATCTTACTTTGTACTGATGTTGCTGCTCGTGGCTTGGATATCCCCGCCGTGGACTGGATTGTGCAGTTTGATCCTCCAGATGAACCCAAGGAATATATCCACAGGGTTGGTCGAACAGCTCGTGGGGAAGGTGGAAAAGGAAATGCACTACTTTTCCTCATTCCTGAAGAGTTGCAGTTTCTTCGCTATTTGAGGGAGGCAAAAGTCCCTGTTAAAGAGTATGAATTTAATGAGAAGAAGCTAAAAAATGTGCAATCTCAGCTGGAGAAGTTGGTTGCCAACAACTACCATCTGAACCGTGCAGCAAAAGATGCATATAGGTCTTATCTATTAGCATATAATTCACACTCTATGAAGGACATCTTTAACGTTCACCGACTCGATCTCCAGGCAGTTGCTTCTTCATTTTGCTTTTCTAATCCACCGAAGGTAAATTTAAACTTAGACAGCAGTGCATCAAAGTTCCGGAAGAACATGCGTAGAGTGGATGGAGTTCGACATGGAATCAATCCTAACAATCCTTATGGAAGGCAAAGAGGTGGAGATGATGACATGAGACAATTTGTGAGACATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

464

Amino Acids

52.28

Weight (kDa)

9.43

Isoelectric Point (pI)

34.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	2 - 154	2.1e-40	DEAD/DEAH box helicase
ResIII	PF04851	21 - 155	3e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	211 - 314	1e-23	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	368 - 417	2.5e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18600
fragaria_vesca	FvH4_5g12870
malus_domestica	MD05G1177700.v1.1 MD06G1209500.v1.1 MD14G1220200.v1.1 MD15G1015500.v1.1
prunus_persica	Prupe.5G214700_v2.0.a1 Prupe.6G096100_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G097300_v2.0.a1
pyrus_communis	pycom05g16360 pycom05g16400 pycom05g16410 pycom06g18710 pycom14g18230 pycom15g01320
rosa_chinensis	RchiOBHm_Chr5g0083351 RchiOBHm_Chr5g0083361 RchiOBHm_Chr7g0184361
rosa_laevigata	RLG00000004982 RLG00000037030
rosa_multiflora	Rmu_sc0001444.1_g000013 Rmu_sc0001557.1_g000022 Rmu_sc0002245.1_g000045
rosa_roxburghii	Rroxscaffold_1G00001130 Rroxscaffold_3G00269690
rosa_samantha	Rh5AG533800 Rh5BG561600 Rh5CG583900 Rh5CG584000 Rh7AG080700 Rh7BG066500 Rh7CG066800 Rh7DG066500
rosa_wichuraiana	Rw5G049900 Rw7G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	100
AccB7I	CCANNNNNTGG	1 cut(s)	205
AccIII	TCCGGA	1 cut(s)	1284
AciI	CCGC	2 cut(s)	32, 864
AclI	AACGTT	1 cut(s)	1188
AclWI	GGATC	1 cut(s)	884
AcoI	YGGCCR	1 cut(s)	207
AcsI	RAATTY	3 cut(s)	704, 1043, 1252
AcuI	CTGAAG	1 cut(s)	1005
AfaI	GTAC	2 cut(s)	787, 832
AfiI	CCNNNNNNNGG	5 cut(s)	146, 178, 205, 868, 873
AgsI	TTSAA	4 cut(s)	14, 379, 478, 503
AjnI	CCWGG	2 cut(s)	322, 1206
AluBI	AGCT	7 cut(s)	62, 107, 132, 401, 938, 1060, 1081
AluI	AGCT	7 cut(s)	62, 107, 132, 401, 938, 1060, 1081
Alw26I	GTCTC	2 cut(s)	1369, 1381
AlwI	GGATC	1 cut(s)	884
Aor13HI	TCCGGA	1 cut(s)	1284
AoxI	GGCC	1 cut(s)	207
ApeKI	GCWGC	5 cut(s)	62, 586, 769, 842, 1121
ApoI	RAATTY	3 cut(s)	704, 1043, 1252
ArsI	GACNNNNNNTTYG	2 cut(s)	229, 261
AseI	ATTAAT	1 cut(s)	732
AspS9I	GGNCC	2 cut(s)	75, 214
AsuC2I	CCSGG	1 cut(s)	79
AsuHPI	GGTGA	1 cut(s)	1184
AvaII	GGWCC	2 cut(s)	75, 214
BalI	TGGCCA	1 cut(s)	209
BanI	GGYRCC	1 cut(s)	100
BauI	CACGAG	2 cut(s)	846, 939
BbsI	GAAGAC	2 cut(s)	89, 670
BbvI	GCAGC	5 cut(s)	49, 573, 781, 829, 1133
BccI	CCATC	5 cut(s)	348, 551, 667, 1116, 1301
BceAI	ACGGC	2 cut(s)	188, 851
BcgI	CGANNNNNNTGC	4 cut(s)	117, 151, 983, 1017
BciT130I	CCWGG	2 cut(s)	324, 1208
BciVI	GTATCC	1 cut(s)	402
BcnI	CCSGG	1 cut(s)	79
BcoDI	GTCTC	2 cut(s)	1369, 1381
BfaI	CTAG	2 cut(s)	177, 267
BfmI	CTRYAG	1 cut(s)	344
BfuI	GTATCC	1 cut(s)	402
BglII	AGATCT	1 cut(s)	505
BisI	GCNGC	5 cut(s)	63, 587, 770, 843, 1122
BlsI	GCNGC	5 cut(s)	64, 588, 771, 844, 1123
BmcAI	AGTACT	1 cut(s)	787
Bme1390I	CCNGG	3 cut(s)	79, 324, 1208
Bme18I	GGWCC	2 cut(s)	75, 214
BmgT120I	GGNCC	2 cut(s)	75, 214
BmiI	GGNNCC	2 cut(s)	76, 102
BmrFI	CCNGG	3 cut(s)	79, 324, 1208
BmrI	ACTGGG	1 cut(s)	494
BmsI	GCATC	2 cut(s)	1120, 1283
BmuI	ACTGGG	1 cut(s)	494
BpiI	GAAGAC	2 cut(s)	89, 670
BpmI	CTGGAG	3 cut(s)	879, 1103, 1190
BpuEI	CTTGAG	1 cut(s)	206
BpuMI	CCSGG	1 cut(s)	79
BsaJI	CCNNGG	6 cut(s)	204, 210, 354, 754, 867, 906
BsaWI	WCCGGW	1 cut(s)	1284
BsaXI	ACNNNNNCTCC	2 cut(s)	1188, 1218
Bsc4I	CCNNNNNNNGG	5 cut(s)	146, 178, 205, 868, 873
Bse1I	ACTGG	4 cut(s)	157, 417, 489, 878
BseAI	TCCGGA	1 cut(s)	1284
BseBI	CCWGG	2 cut(s)	324, 1208
BseDI	CCNNGG	6 cut(s)	204, 210, 354, 754, 867, 906
BseGI	GGATG	2 cut(s)	559, 1312
BseLI	CCNNNNNNNGG	5 cut(s)	146, 178, 205, 868, 873
BseMII	CTCAG	3 cut(s)	20, 711, 1091
BseNI	ACTGG	4 cut(s)	157, 417, 489, 878
BseRI	GAGGAG	1 cut(s)	288
BseXI	GCAGC	5 cut(s)	49, 573, 781, 829, 1133
BsgI	GTGCAG	2 cut(s)	902, 1140
BshFI	GGCC	1 cut(s)	209
BshNI	GGYRCC	1 cut(s)	100
BsiSI	CCGG	2 cut(s)	78, 1285
BslFI	GGGAC	2 cut(s)	181, 1010
BslI	CCNNNNNNNGG	5 cut(s)	146, 178, 205, 868, 873
BsmAI	GTCTC	2 cut(s)	1369, 1381
BsmFI	GGGAC	2 cut(s)	181, 1010
BsnI	GGCC	1 cut(s)	209
Bsp13I	TCCGGA	1 cut(s)	1284
Bsp143I	GATC	4 cut(s)	39, 505, 889, 1201
Bsp19I	CCATGG	1 cut(s)	754
BspACI	CCGC	2 cut(s)	32, 864
BspANI	GGCC	1 cut(s)	209
BspCNI	CTCAG	3 cut(s)	19, 710, 1090
BspEI	TCCGGA	1 cut(s)	1284
BspLI	GGNNCC	2 cut(s)	76, 102
BspMAI	CTGCAG	1 cut(s)	348
BspPI	GGATC	1 cut(s)	884
BspT107I	GGYRCC	1 cut(s)	100
BsrI	ACTGG	4 cut(s)	157, 417, 489, 878
BssECI	CCNNGG	6 cut(s)	204, 210, 354, 754, 867, 906
BssMI	GATC	4 cut(s)	39, 505, 889, 1201
BssSI	CACGAG	2 cut(s)	846, 939
BssT1I	CCWWGG	4 cut(s)	210, 354, 754, 906
Bst2BI	CACGAG	2 cut(s)	846, 939
Bst2UI	CCWGG	2 cut(s)	324, 1208
Bst4CI	ACNGT	2 cut(s)	195, 1118
Bst6I	CTCTTC	5 cut(s)	40, 265, 691, 770, 981
BstAPI	GCANNNNNTGC	1 cut(s)	1130
BstC8I	GCNNGC	3 cut(s)	105, 130, 514
BstDEI	CTNAG	6 cut(s)	6, 567, 697, 722, 1077, 1261
BstDSI	CCRYGG	3 cut(s)	204, 754, 867
BstF5I	GGATG	2 cut(s)	559, 1312
BstKTI	GATC	4 cut(s)	42, 508, 892, 1204
BstMAI	GTCTC	2 cut(s)	1369, 1381
BstMBI	GATC	4 cut(s)	39, 505, 889, 1201
BstMWI	GCNNNNNNNGC	3 cut(s)	769, 1078, 1130
BstNI	CCWGG	2 cut(s)	324, 1208
BstNSI	RCATGY	1 cut(s)	1297
BstSCI	CCNGG	3 cut(s)	77, 322, 1206
BstSFI	CTRYAG	1 cut(s)	344
BstV1I	GCAGC	5 cut(s)	49, 573, 781, 829, 1133
BstV2I	GAAGAC	2 cut(s)	89, 670
BstX2I	RGATCY	1 cut(s)	505
BstXI	CCANNNNNNTGG	1 cut(s)	926
BstYI	RGATCY	1 cut(s)	505
BsuI	GTATCC	1 cut(s)	402
BsuRI	GGCC	1 cut(s)	209
BtgI	CCRYGG	3 cut(s)	204, 754, 867
BtsCI	GGATG	2 cut(s)	559, 1312
BtsI	GCAGTG	1 cut(s)	1276
BtsIMutI	CAGTG	2 cut(s)	705, 1276
Cac8I	GCNNGC	3 cut(s)	105, 130, 514
Cfr13I	GGNCC	2 cut(s)	75, 214
CseI	GACGC	1 cut(s)	275
Csp6I	GTAC	2 cut(s)	786, 831
CspCI	CAANNNNNGTGG	2 cut(s)	18, 53
CviAII	CATG	5 cut(s)	200, 755, 1294, 1319, 1372
CviQI	GTAC	2 cut(s)	786, 831
DdeI	CTNAG	6 cut(s)	6, 567, 697, 722, 1077, 1261
DpnI	GATC	4 cut(s)	41, 507, 891, 1203
DpnII	GATC	4 cut(s)	39, 505, 889, 1201
DraI	TTTAAA	1 cut(s)	1257
EaeI	YGGCCR	1 cut(s)	207
Eam1104I	CTCTTC	5 cut(s)	40, 265, 691, 770, 981
EarI	CTCTTC	5 cut(s)	40, 265, 691, 770, 981
Eco130I	CCWWGG	4 cut(s)	210, 354, 754, 906
Eco32I	GATATC	2 cut(s)	751, 859
Eco47I	GGWCC	2 cut(s)	75, 214
Eco57I	CTGAAG	1 cut(s)	1005
EcoO109I	RGGNCCY	1 cut(s)	214
EcoRII	CCWGG	2 cut(s)	322, 1206
EcoRV	GATATC	2 cut(s)	751, 859
EcoT14I	CCWWGG	4 cut(s)	210, 354, 754, 906
EcoT22I	ATGCAT	1 cut(s)	1135
ErhI	CCWWGG	4 cut(s)	210, 354, 754, 906
FaeI	CATG	5 cut(s)	203, 758, 1297, 1322, 1375
FalI	AAGNNNNNCTT	2 cut(s)	1166, 1198
FaqI	GGGAC	2 cut(s)	181, 1010
FatI	CATG	5 cut(s)	199, 754, 1293, 1318, 1371
FauI	CCCGC	1 cut(s)	871
Fnu4HI	GCNGC	5 cut(s)	63, 587, 770, 843, 1122
FokI	GGATG	2 cut(s)	566, 1319
Fsp4HI	GCNGC	5 cut(s)	63, 587, 770, 843, 1122
FspBI	CTAG	2 cut(s)	177, 267
GluI	GCNGC	5 cut(s)	63, 587, 770, 843, 1122
GsuI	CTGGAG	3 cut(s)	879, 1103, 1190
HaeIII	GGCC	1 cut(s)	209
HapII	CCGG	2 cut(s)	78, 1285
HgaI	GACGC	1 cut(s)	275
Hin1II	CATG	5 cut(s)	203, 758, 1297, 1322, 1375
HindIII	AAGCTT	1 cut(s)	130
HpaII	CCGG	2 cut(s)	78, 1285
HphI	GGTGA	1 cut(s)	1184
Hpy166II	GTNNAC	3 cut(s)	304, 871, 1192
Hpy188I	TCNGA	4 cut(s)	9, 44, 715, 1113
Hpy188III	TCNNGA	9 cut(s)	18, 223, 335, 526, 574, 646, 896, 983, 1285
Hpy8I	GTNNAC	3 cut(s)	304, 871, 1192
HpyCH4III	ACNGT	2 cut(s)	195, 1118
HpyCH4IV	ACGT	1 cut(s)	1188
HpyF10VI	GCNNNNNNNGC	3 cut(s)	769, 1078, 1130
HpyF3I	CTNAG	6 cut(s)	6, 567, 697, 722, 1077, 1261
HpySE526I	ACGT	1 cut(s)	1188
Hsp92II	CATG	5 cut(s)	203, 758, 1297, 1322, 1375
Kpn2I	TCCGGA	1 cut(s)	1284
Kzo9I	GATC	4 cut(s)	39, 505, 889, 1201
LmnI	GCTCC	1 cut(s)	59
Lsp1109I	GCAGC	5 cut(s)	49, 573, 781, 829, 1133
LweI	GCATC	2 cut(s)	1120, 1283
MaeI	CTAG	2 cut(s)	177, 267
MaeII	ACGT	1 cut(s)	1188
MaeIII	GTNAC	2 cut(s)	571, 692
MalI	GATC	4 cut(s)	41, 507, 891, 1203
MboI	GATC	4 cut(s)	39, 505, 889, 1201
MflI	RGATCY	1 cut(s)	505
MlsI	TGGCCA	1 cut(s)	209
MluCI	AATT	6 cut(s)	472, 704, 1043, 1159, 1252, 1379
MluNI	TGGCCA	1 cut(s)	209
MlyI	GAGTC	3 cut(s)	79, 232, 1191
MmeI	TCCRAC	1 cut(s)	738
Mox20I	TGGCCA	1 cut(s)	209
Mph1103I	ATGCAT	1 cut(s)	1135
MroI	TCCGGA	1 cut(s)	1284
MscI	TGGCCA	1 cut(s)	209
MseI	TTAA	6 cut(s)	450, 732, 1032, 1047, 1185, 1256
MslI	CAYNNNNRTG	1 cut(s)	734
Msp20I	TGGCCA	1 cut(s)	209
MspA1I	CMGCKG	3 cut(s)	34, 107, 1081
MspI	CCGG	2 cut(s)	78, 1285
MspR9I	CCNGG	3 cut(s)	79, 324, 1208
MvaI	CCWGG	2 cut(s)	324, 1208
MwoI	GCNNNNNNNGC	3 cut(s)	769, 1078, 1130
NciI	CCSGG	1 cut(s)	79
NcoI	CCATGG	1 cut(s)	754
NdeII	GATC	4 cut(s)	39, 505, 889, 1201
NlaIII	CATG	5 cut(s)	203, 758, 1297, 1322, 1375
NlaIV	GGNNCC	2 cut(s)	76, 102
NmuCI	GTSAC	1 cut(s)	571
NsiI	ATGCAT	1 cut(s)	1135
NspI	RCATGY	1 cut(s)	1297
PcsI	WCGNNNNNNNCGW	1 cut(s)	937
PfeI	GAWTC	6 cut(s)	10, 226, 360, 655, 819, 1323
PflFI	GACNNNGTC	1 cut(s)	73
PflMI	CCANNNNNTGG	1 cut(s)	205
PfoI	TCCNGGA	1 cut(s)	77
PkrI	GCNGC	5 cut(s)	64, 588, 771, 844, 1123
PleI	GAGTC	3 cut(s)	79, 232, 1191
PpsI	GAGTC	3 cut(s)	79, 232, 1191
PpuMI	RGGWCCY	1 cut(s)	214
PshBI	ATTAAT	1 cut(s)	732
Psp1406I	AACGTT	1 cut(s)	1188
Psp5II	RGGWCCY	1 cut(s)	214
Psp6I	CCWGG	2 cut(s)	322, 1206
PspGI	CCWGG	2 cut(s)	322, 1206
PspN4I	GGNNCC	2 cut(s)	76, 102
PspPI	GGNCC	2 cut(s)	75, 214
PspPPI	RGGWCCY	1 cut(s)	214
PsrI	GAACNNNNNNTAC	2 cut(s)	174, 206
PstI	CTGCAG	1 cut(s)	348
PsuI	RGATCY	1 cut(s)	505
PsyI	GACNNNGTC	1 cut(s)	73
PvuII	CAGCTG	2 cut(s)	107, 1081
RsaI	GTAC	2 cut(s)	787, 832
RsaNI	GTAC	2 cut(s)	786, 831
RseI	CAYNNNNRTG	1 cut(s)	734
SaqAI	TTAA	6 cut(s)	450, 732, 1032, 1047, 1185, 1256
SatI	GCNGC	5 cut(s)	63, 587, 770, 843, 1122
Sau3AI	GATC	4 cut(s)	39, 505, 889, 1201
Sau96I	GGNCC	2 cut(s)	75, 214
ScaI	AGTACT	1 cut(s)	787
SchI	GAGTC	3 cut(s)	79, 232, 1191
ScrFI	CCNGG	3 cut(s)	79, 324, 1208
SfaNI	GCATC	2 cut(s)	1120, 1283
SfcI	CTRYAG	1 cut(s)	344
SinI	GGWCC	2 cut(s)	75, 214
SmiMI	CAYNNNNRTG	1 cut(s)	734
SmlI	CTYRAG	1 cut(s)	221
SmoI	CTYRAG	1 cut(s)	221
Sse9I	AATT	6 cut(s)	472, 704, 1043, 1159, 1252, 1379
SsiI	CCGC	2 cut(s)	32, 864
SspMI	CTAG	2 cut(s)	177, 267
StyD4I	CCNGG	3 cut(s)	77, 322, 1206
StyI	CCWWGG	4 cut(s)	210, 354, 754, 906
TaaI	ACNGT	2 cut(s)	195, 1118
TaiI	ACGT	1 cut(s)	1191
TaqI	TCGA	3 cut(s)	931, 1200, 1315
TasI	AATT	6 cut(s)	472, 704, 1043, 1159, 1252, 1379
TatI	WGTACW	2 cut(s)	785, 830
TfiI	GAWTC	6 cut(s)	10, 226, 360, 655, 819, 1323
Tru1I	TTAA	6 cut(s)	450, 732, 1032, 1047, 1185, 1256
Tru9I	TTAA	6 cut(s)	450, 732, 1032, 1047, 1185, 1256
TscAI	CASTG	2 cut(s)	705, 1276
TseFI	GTSAC	1 cut(s)	571
TseI	GCWGC	5 cut(s)	62, 586, 769, 842, 1121
Tsp45I	GTSAC	1 cut(s)	571
TspDTI	ATGAA	7 cut(s)	411, 452, 594, 915, 1056, 1187, 1212
TspRI	CASTG	2 cut(s)	705, 1276
Tth111I	GACNNNGTC	1 cut(s)	73
Van91I	CCANNNNNTGG	1 cut(s)	205
VpaK11BI	GGWCC	2 cut(s)	75, 214
VspI	ATTAAT	1 cut(s)	732
XapI	RAATTY	3 cut(s)	704, 1043, 1252
XceI	RCATGY	1 cut(s)	1297
XspI	CTAG	2 cut(s)	177, 267
ZrmI	AGTACT	1 cut(s)	787
Zsp2I	ATGCAT	1 cut(s)	1135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.