Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003249

Physical Location & Seq

Genomic Coordinates Forward (+)

30234310 .. 30238583

Gene Structure 4274 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1220200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGACAGACCCAAACCTTCACGCGCATTCGGAGGACGAGACGAAGAAGAAGAAGCGCAAGAGAAACAGAGCGAAGAAACCGTCGGAATCTGCGGGTGTGGAGAAAGAAGAATATACGGAGCCTCAAAACCCTAACGGCGAACCGGCTAAGGGCGAGAGTGAGAAGAAGAGGAAGAAGGTGAAGAAATTGAAGGGAGAGGAAGTAGAGGATCAAAGCCCCGTGGAGGACAAAGATGAAGAAGATGAGGAGAGGGAAGAAGCAATGGAGGAGGAGGAGGAGAAGAAGAAGAAGAAGAAGGTGAATAGTGGCGGTGGAGGGACTGGGATTATGACCACAGAGTCGTTTGCTTCCTTGAACTTGTCTGAGAACACTTACAAGGCCATTGAAGAAATGAAGTTTCAGTATATGACTCAGATTCAAGCTAGAGCAATCCCACCGCTCTTGATTGGAAAAGATGTCCTCGGAGCTGCAAGGACAGGGTCTGGTAAAACTCTTGCTTTTCTCATACCAGCTGTGGAGCTGCTATATAACACACATTTTGCTCCTCGTAACGGAATGGGTGTTGTTGTGATTTGCCCAACACGGGAACTTGCAATACAGACCCATGCTGTGGCAAAGGACCTTCTAAAGTATCACTCTCAGACTCTTGGCTTGGTTATTGGAGGTTCAGCTAGGAGAGGGGAAGCAGAACGTATTGTAAAAGGAGTGAATTTATTGGTTGCAACCCCTGGTCGACTTCTTGACCATCTTCAGAATACCAAAGGATTCATATATAAGAACTTGAAGTGCCTCGTGATTGATGAAGCTGACAGGTTATTGGAAGCGAACTTTGAGGAGGAAATGAAACAGATTGTCAAGATTCTGCCAAAGGATAGGCAAACAGCTTTATTTTCAGCAACCCAAACTAAGAAGGTTGAAGATCTTGTTAAGTTGTCATTTCGAAAAGATCAGAAACCTATCTATGTTGATGTGGATGAGGGGAGGTCAAAGGTCACCAATGAAGGGCTGGAACAAGGCTATTGTGTTGTCCCAAGTGCGAAGAGATTTCTTCTCTTATATTCTTTCCTGACAAAGAATACATCTAAGAAAGTTATGGTCTTCTTCTCGTCTTGTAACTCAGTGAAATTCCACTCCGAACTTCTTAGATACGTTAATGTGGAATGCTTTGATATCCATGGAAAGCAAAAGCAGCAGAAGAGGACAACTACTTTTTTTGACTTCTGTAAAGCAGAGAAAGGAATCTTACTTTGTACTGATGTTGCTGCTCGTGGACTGGATATTCCTGCTGTGGACTGGATTTTGCAGTTTGATCCTCCGGATGAACCGACGGAATATATCCATAGGGTTGGTCGAACAGCTCGAGGGGAAGGTGCAACAGGAAAGGCATTACTTTTCCTCATTCCCGAAGAGTTGCAATTCATTCGCTATTTGAAGGCTGCAAATGTGCCCGTTAAACCATACGATTTTAATGAAAAGAAGCTGAAGAATGTGCAGTCAAAACTGGAGCAGATGGTTCAAAACATTCACTACCTGAATAAATCAGCAAAAGAGGCGTATACATCTTATTTATTAGCATACAATTCACACTCTATGAAGGACATCTTTAATGTACACCGGCTTGATCTGCAGGCGGTTGCAGCTTCTTTTTGTTTTTCTAATCCGCCCAAGGTGCATTTGAACTTAGACAGCAGTAAATCGAAGTTCAGGAAGAAGACGCGTAAAGAAGGAAGTCGAAATGGATTCAGTGAGAGCAATCCTTACGGCAATCGAAAAGGCGGAGATGATAGACAGTTCATAAGGCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

602

Amino Acids

68.08

Weight (kDa)

9.17

Isoelectric Point (pI)

39.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	137 - 308	1.9e-47	DEAD/DEAH box helicase
ResIII	PF04851	154 - 300	3.4e-07	Type III restriction enzyme, res subunit
Helicase_C	PF00271	351 - 454	7.8e-25	Helicase conserved C-terminal domain
CTE_SPB4	PF13959	510 - 557	3.4e-17	ATP-dependent rRNA helicase SPB4-like, C-terminal extension

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18600
fragaria_vesca	FvH4_5g12870
malus_domestica	MD05G1177700.v1.1 MD06G1209500.v1.1 MD14G1220200.v1.1 MD15G1015500.v1.1
prunus_persica	Prupe.5G214700_v2.0.a1 Prupe.6G096100_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G097300_v2.0.a1
pyrus_communis	pycom05g16360 pycom05g16400 pycom05g16410 pycom06g18710 pycom14g18230 pycom15g01320
rosa_chinensis	RchiOBHm_Chr5g0083351 RchiOBHm_Chr5g0083361 RchiOBHm_Chr7g0184361
rosa_laevigata	RLG00000004982 RLG00000037030
rosa_multiflora	Rmu_sc0001444.1_g000013 Rmu_sc0001557.1_g000022 Rmu_sc0002245.1_g000045
rosa_roxburghii	Rroxscaffold_1G00001130 Rroxscaffold_3G00269690
rosa_samantha	Rh5AG533800 Rh5BG561600 Rh5CG583900 Rh5CG584000 Rh7AG080700 Rh7BG066500 Rh7CG066800 Rh7DG066500
rosa_wichuraiana	Rw5G049900 Rw7G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	337
AccB7I	CCANNNNNTGG	1 cut(s)	610
AccBSI	CCGCTC	1 cut(s)	439
AccI	GTMKAC	2 cut(s)	733, 1556
AccII	CGCG	2 cut(s)	23, 1717
AccIII	TCCGGA	1 cut(s)	1315
AciI	CCGC	6 cut(s)	92, 309, 437, 1631, 1661, 1776
AclWI	GGATC	2 cut(s)	216, 1304
AcsI	RAATTY	2 cut(s)	709, 1124
AcuI	CTGAAG	2 cut(s)	734, 1502
AfaI	GTAC	2 cut(s)	1252, 1611
AfiI	CCNNNNNNNGG	6 cut(s)	149, 223, 551, 583, 610, 1002
AflIII	ACRYGT	1 cut(s)	1715
AgsI	TTSAA	9 cut(s)	190, 355, 386, 419, 784, 917, 1432, 1517, 1678
AjnI	CCWGG	1 cut(s)	727
Alw26I	GTCTC	1 cut(s)	32
AlwI	GGATC	2 cut(s)	216, 1304
AlwNI	CAGNNNCTG	1 cut(s)	482
Ama87I	CYCGRG	1 cut(s)	1359
Aor13HI	TCCGGA	1 cut(s)	1315
AoxI	GGCC	1 cut(s)	378
ApeKI	GCWGC	6 cut(s)	467, 520, 1189, 1262, 1436, 1637
ApoI	RAATTY	2 cut(s)	709, 1124
ArsI	GACNNNNNNTTYG	2 cut(s)	65, 97
AspLEI	GCGC	2 cut(s)	25, 57
AspS9I	GGNCC	1 cut(s)	619
AsuHPI	GGTGA	3 cut(s)	190, 310, 985
AsuII	TTCGAA	1 cut(s)	940
AvaI	CYCGRG	1 cut(s)	1359
AvaII	GGWCC	1 cut(s)	619
BaeGI	GKGCMC	1 cut(s)	1449
BauI	CACGAG	2 cut(s)	791, 1266
BbsI	GAAGAC	2 cut(s)	1090, 1718
BbvI	GCAGC	6 cut(s)	454, 507, 1201, 1249, 1423, 1649
BccI	CCATC	2 cut(s)	753, 1504
BceAI	ACGGC	2 cut(s)	151, 1777
BcgI	CGANNNNNNTGC	2 cut(s)	1403, 1437
BciT130I	CCWGG	1 cut(s)	729
BcoDI	GTCTC	1 cut(s)	32
BfaI	CTAG	2 cut(s)	423, 672
BfmI	CTRYAG	1 cut(s)	1625
BglII	AGATCT	1 cut(s)	919
BisI	GCNGC	6 cut(s)	468, 521, 1190, 1263, 1437, 1638
BlsI	GCNGC	6 cut(s)	469, 522, 1191, 1264, 1438, 1639
Bme1390I	CCNGG	1 cut(s)	729
Bme18I	GGWCC	1 cut(s)	619
BmeT110I	CYCGRG	1 cut(s)	1359
BmgT120I	GGNCC	1 cut(s)	619
BmiI	GGNNCC	1 cut(s)	120
BmrFI	CCNGG	1 cut(s)	729
BmrI	ACTGGG	1 cut(s)	330
BmuI	ACTGGG	1 cut(s)	330
BpiI	GAAGAC	2 cut(s)	1090, 1718
BpmI	CTGGAG	1 cut(s)	1523
Bpu10I	CCTNAGC	1 cut(s)	147
Bpu14I	TTCGAA	1 cut(s)	940
BsaJI	CCNNGG	5 cut(s)	219, 460, 727, 1174, 1665
BsaWI	WCCGGW	1 cut(s)	1315
BsaXI	ACNNNNNCTCC	2 cut(s)	186, 216
Bsc4I	CCNNNNNNNGG	6 cut(s)	149, 223, 551, 583, 610, 1002
Bse118I	RCCGGY	2 cut(s)	142, 1614
Bse1I	ACTGG	4 cut(s)	325, 1278, 1298, 1506
Bse3DI	GCAATG	1 cut(s)	267
BseAI	TCCGGA	1 cut(s)	1315
BseBI	CCWGG	1 cut(s)	729
BseDI	CCNNGG	5 cut(s)	219, 460, 727, 1174, 1665
BseGI	GGATG	2 cut(s)	979, 1324
BseLI	CCNNNNNNNGG	6 cut(s)	149, 223, 551, 583, 610, 1002
BseMI	GCAATG	1 cut(s)	267
BseMII	CTCAG	4 cut(s)	354, 425, 653, 1131
BseNI	ACTGG	4 cut(s)	325, 1278, 1298, 1506
BseRI	GAGGAG	7 cut(s)	260, 281, 284, 287, 290, 534, 848
BseSI	GKGCMC	1 cut(s)	1449
BseXI	GCAGC	6 cut(s)	454, 507, 1201, 1249, 1423, 1649
BsgI	GTGCAG	1 cut(s)	1511
Bsh1236I	CGCG	2 cut(s)	23, 1717
BshFI	GGCC	1 cut(s)	380
BsiHKCI	CYCGRG	1 cut(s)	1359
BsiSI	CCGG	3 cut(s)	143, 1316, 1615
BslFI	GGGAC	2 cut(s)	331, 1013
BslI	CCNNNNNNNGG	6 cut(s)	149, 223, 551, 583, 610, 1002
BsmAI	GTCTC	1 cut(s)	32
BsmBI	CGTCTC	1 cut(s)	32
BsmFI	GGGAC	2 cut(s)	331, 1013
BsmI	GAATGC	2 cut(s)	25, 1166
BsnI	GGCC	1 cut(s)	380
BsoBI	CYCGRG	1 cut(s)	1359
Bsp119I	TTCGAA	1 cut(s)	940
Bsp1286I	GDGCHC	1 cut(s)	1449
Bsp13I	TCCGGA	1 cut(s)	1315
Bsp1407I	TGTACA	1 cut(s)	1609
Bsp143I	GATC	5 cut(s)	208, 919, 946, 1309, 1621
Bsp19I	CCATGG	1 cut(s)	1174
BspACI	CCGC	6 cut(s)	92, 309, 437, 1631, 1661, 1776
BspANI	GGCC	1 cut(s)	380
BspCNI	CTCAG	4 cut(s)	355, 424, 652, 1130
BspEI	TCCGGA	1 cut(s)	1315
BspFNI	CGCG	2 cut(s)	23, 1717
BspLI	GGNNCC	1 cut(s)	120
BspMAI	CTGCAG	1 cut(s)	1629
BspPI	GGATC	2 cut(s)	216, 1304
BspT104I	TTCGAA	1 cut(s)	940
BsrBI	CCGCTC	1 cut(s)	439
BsrDI	GCAATG	1 cut(s)	267
BsrFI	RCCGGY	2 cut(s)	142, 1614
BsrGI	TGTACA	1 cut(s)	1609
BsrI	ACTGG	4 cut(s)	325, 1278, 1298, 1506
BssAI	RCCGGY	2 cut(s)	142, 1614
BssECI	CCNNGG	5 cut(s)	219, 460, 727, 1174, 1665
BssMI	GATC	5 cut(s)	208, 919, 946, 1309, 1621
BssNAI	GTATAC	1 cut(s)	1557
BssSI	CACGAG	2 cut(s)	791, 1266
BssT1I	CCWWGG	2 cut(s)	1174, 1665
Bst1107I	GTATAC	1 cut(s)	1557
Bst2BI	CACGAG	2 cut(s)	791, 1266
Bst2UI	CCWGG	1 cut(s)	729
Bst4CI	ACNGT	2 cut(s)	81, 1791
Bst6I	CTCTTC	4 cut(s)	161, 1034, 1190, 1401
BstAUI	TGTACA	1 cut(s)	1609
BstBI	TTCGAA	1 cut(s)	940
BstC8I	GCNNGC	1 cut(s)	1629
BstDEI	CTNAG	9 cut(s)	147, 363, 411, 639, 906, 1083, 1117, 1142, 1681
BstDSI	CCRYGG	2 cut(s)	219, 1174
BstEII	GGTNACC	1 cut(s)	991
BstF5I	GGATG	2 cut(s)	979, 1324
BstFNI	CGCG	2 cut(s)	23, 1717
BstHHI	GCGC	2 cut(s)	25, 57
BstKTI	GATC	5 cut(s)	211, 922, 949, 1312, 1624
BstMAI	GTCTC	1 cut(s)	32
BstMBI	GATC	5 cut(s)	208, 919, 946, 1309, 1621
BstMWI	GCNNNNNNNGC	5 cut(s)	1189, 1550, 1624, 1637, 1669
BstNI	CCWGG	1 cut(s)	729
BstPI	GGTNACC	1 cut(s)	991
BstSCI	CCNGG	1 cut(s)	727
BstSFI	CTRYAG	1 cut(s)	1625
BstSLI	GKGCMC	1 cut(s)	1449
BstUI	CGCG	2 cut(s)	23, 1717
BstV1I	GCAGC	6 cut(s)	454, 507, 1201, 1249, 1423, 1649
BstV2I	GAAGAC	2 cut(s)	1090, 1718
BstX2I	RGATCY	1 cut(s)	919
BstXI	CCANNNNNNTGG	1 cut(s)	1346
BstYI	RGATCY	1 cut(s)	919
BstZ17I	GTATAC	1 cut(s)	1557
BsuRI	GGCC	1 cut(s)	380
BtgI	CCRYGG	2 cut(s)	219, 1174
BtsCI	GGATG	2 cut(s)	979, 1324
BtsIMutI	CAGTG	2 cut(s)	1125, 1750
Cac8I	GCNNGC	1 cut(s)	1629
CaiI	CAGNNNCTG	1 cut(s)	482
CfoI	GCGC	2 cut(s)	25, 57
Cfr10I	RCCGGY	2 cut(s)	142, 1614
Cfr13I	GGNCC	1 cut(s)	619
CseI	GACGC	1 cut(s)	1723
Csp6I	GTAC	2 cut(s)	1251, 1610
CspCI	CAANNNNNGTGG	2 cut(s)	423, 458
CviAII	CATG	2 cut(s)	605, 1175
CviQI	GTAC	2 cut(s)	1251, 1610
DdeI	CTNAG	9 cut(s)	147, 363, 411, 639, 906, 1083, 1117, 1142, 1681
DpnI	GATC	5 cut(s)	210, 921, 948, 1311, 1623
DpnII	GATC	5 cut(s)	208, 919, 946, 1309, 1621
DrdI	GACNNNNNNGTC	1 cut(s)	337
DseDI	GACNNNNNNGTC	1 cut(s)	337
Eam1104I	CTCTTC	4 cut(s)	161, 1034, 1190, 1401
EarI	CTCTTC	4 cut(s)	161, 1034, 1190, 1401
EciI	GGCGGA	2 cut(s)	1650, 1791
Eco130I	CCWWGG	2 cut(s)	1174, 1665
Eco32I	GATATC	1 cut(s)	1171
Eco47I	GGWCC	1 cut(s)	619
Eco57I	CTGAAG	2 cut(s)	734, 1502
Eco88I	CYCGRG	1 cut(s)	1359
Eco91I	GGTNACC	1 cut(s)	991
EcoO109I	RGGNCCY	1 cut(s)	619
EcoO65I	GGTNACC	1 cut(s)	991
EcoRII	CCWGG	1 cut(s)	727
EcoRV	GATATC	1 cut(s)	1171
EcoT14I	CCWWGG	2 cut(s)	1174, 1665
ErhI	CCWWGG	2 cut(s)	1174, 1665
Esp3I	CGTCTC	1 cut(s)	32
FaeI	CATG	2 cut(s)	608, 1178
FalI	AAGNNNNNCTT	2 cut(s)	1586, 1618
FaqI	GGGAC	2 cut(s)	331, 1013
FatI	CATG	2 cut(s)	604, 1174
FauI	CCCGC	1 cut(s)	85
FblI	GTMKAC	2 cut(s)	733, 1556
Fnu4HI	GCNGC	6 cut(s)	468, 521, 1190, 1263, 1437, 1638
FokI	GGATG	2 cut(s)	986, 1331
Fsp4HI	GCNGC	6 cut(s)	468, 521, 1190, 1263, 1437, 1638
FspBI	CTAG	2 cut(s)	423, 672
GlaI	GCGC	2 cut(s)	24, 56
GluI	GCNGC	6 cut(s)	468, 521, 1190, 1263, 1437, 1638
GsuI	CTGGAG	1 cut(s)	1523
HaeIII	GGCC	1 cut(s)	380
HapII	CCGG	3 cut(s)	143, 1316, 1615
HgaI	GACGC	1 cut(s)	1723
HhaI	GCGC	2 cut(s)	25, 57
Hin1II	CATG	2 cut(s)	608, 1178
Hin6I	GCGC	2 cut(s)	23, 55
HinP1I	GCGC	2 cut(s)	23, 55
HincII	GTYRAC	1 cut(s)	734
HindII	GTYRAC	1 cut(s)	734
HinfI	GANTC	9 cut(s)	86, 338, 409, 415, 643, 765, 859, 1239, 1740
HpaII	CCGG	3 cut(s)	143, 1316, 1615
HphI	GGTGA	3 cut(s)	190, 310, 985
Hpy166II	GTNNAC	5 cut(s)	734, 1271, 1291, 1557, 1612
Hpy188I	TCNGA	9 cut(s)	31, 85, 364, 414, 464, 642, 753, 951, 1135
Hpy188III	TCNNGA	8 cut(s)	442, 740, 793, 856, 1066, 1316, 1403, 1705
Hpy8I	GTNNAC	5 cut(s)	734, 1271, 1291, 1557, 1612
Hpy99I	CGWCG	2 cut(s)	85, 1330
HpyCH4III	ACNGT	2 cut(s)	81, 1791
HpyCH4IV	ACGT	2 cut(s)	691, 1149
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1189, 1550, 1624, 1637, 1669
HpyF3I	CTNAG	9 cut(s)	147, 363, 411, 639, 906, 1083, 1117, 1142, 1681
HpySE526I	ACGT	2 cut(s)	691, 1149
Hsp92II	CATG	2 cut(s)	608, 1178
HspAI	GCGC	2 cut(s)	23, 55
Kpn2I	TCCGGA	1 cut(s)	1315
Kzo9I	GATC	5 cut(s)	208, 919, 946, 1309, 1621
LmnI	GCTCC	5 cut(s)	118, 464, 517, 547, 1504
Lsp1109I	GCAGC	6 cut(s)	454, 507, 1201, 1249, 1423, 1649
MaeI	CTAG	2 cut(s)	423, 672
MaeII	ACGT	2 cut(s)	691, 1149
MaeIII	GTNAC	3 cut(s)	548, 991, 1112
MalI	GATC	5 cut(s)	210, 921, 948, 1311, 1623
MbiI	CCGCTC	1 cut(s)	439
MboI	GATC	5 cut(s)	208, 919, 946, 1309, 1621
MflI	RGATCY	1 cut(s)	919
MhlI	GDGCHC	1 cut(s)	1449
MluCI	AATT	5 cut(s)	185, 709, 1124, 1415, 1579
MluI	ACGCGT	1 cut(s)	1715
MlyI	GAGTC	3 cut(s)	347, 403, 637
MmeI	TCCRAC	1 cut(s)	63
MroI	TCCGGA	1 cut(s)	1315
MseI	TTAA	5 cut(s)	927, 1152, 1452, 1467, 1605
MspA1I	CMGCKG	1 cut(s)	512
MspI	CCGG	3 cut(s)	143, 1316, 1615
MspR9I	CCNGG	1 cut(s)	729
Mva1269I	GAATGC	2 cut(s)	25, 1166
MvaI	CCWGG	1 cut(s)	729
MvnI	CGCG	2 cut(s)	23, 1717
MwoI	GCNNNNNNNGC	5 cut(s)	1189, 1550, 1624, 1637, 1669
NcoI	CCATGG	1 cut(s)	1174
NdeII	GATC	5 cut(s)	208, 919, 946, 1309, 1621
NlaIII	CATG	2 cut(s)	608, 1178
NlaIV	GGNNCC	1 cut(s)	120
NmuCI	GTSAC	1 cut(s)	991
NspV	TTCGAA	1 cut(s)	940
PaeR7I	CTCGAG	1 cut(s)	1359
PcsI	WCGNNNNNNNCGW	1 cut(s)	1357
PctI	GAATGC	2 cut(s)	25, 1166
PfeI	GAWTC	6 cut(s)	86, 415, 765, 859, 1239, 1740
PflFI	GACNNNGTC	1 cut(s)	478
PflMI	CCANNNNNTGG	1 cut(s)	610
PkrI	GCNGC	6 cut(s)	469, 522, 1191, 1264, 1438, 1639
PleI	GAGTC	3 cut(s)	346, 403, 637
PpsI	GAGTC	3 cut(s)	346, 403, 637
PpuMI	RGGWCCY	1 cut(s)	619
Psp5II	RGGWCCY	1 cut(s)	619
Psp6I	CCWGG	1 cut(s)	727
PspEI	GGTNACC	1 cut(s)	991
PspGI	CCWGG	1 cut(s)	727
PspN4I	GGNNCC	1 cut(s)	120
PspPI	GGNCC	1 cut(s)	619
PspPPI	RGGWCCY	1 cut(s)	619
PspXI	VCTCGAGB	1 cut(s)	1359
PsrI	GAACNNNNNNTAC	2 cut(s)	579, 611
PstI	CTGCAG	1 cut(s)	1629
PstNI	CAGNNNCTG	1 cut(s)	482
PsuI	RGATCY	1 cut(s)	919
PsyI	GACNNNGTC	1 cut(s)	478
PvuII	CAGCTG	1 cut(s)	512
RsaI	GTAC	2 cut(s)	1252, 1611
RsaNI	GTAC	2 cut(s)	1251, 1610
SalI	GTCGAC	1 cut(s)	732
SaqAI	TTAA	5 cut(s)	927, 1152, 1452, 1467, 1605
SatI	GCNGC	6 cut(s)	468, 521, 1190, 1263, 1437, 1638
Sau3AI	GATC	5 cut(s)	208, 919, 946, 1309, 1621
Sau96I	GGNCC	1 cut(s)	619
SchI	GAGTC	3 cut(s)	347, 403, 637
ScrFI	CCNGG	1 cut(s)	729
SduI	GDGCHC	1 cut(s)	1449
SfcI	CTRYAG	1 cut(s)	1625
Sfr274I	CTCGAG	1 cut(s)	1359
SfuI	TTCGAA	1 cut(s)	940
SinI	GGWCC	1 cut(s)	619
SlaI	CTCGAG	1 cut(s)	1359
SmlI	CTYRAG	1 cut(s)	1359
SmoI	CTYRAG	1 cut(s)	1359
Sse9I	AATT	5 cut(s)	185, 709, 1124, 1415, 1579
SsiI	CCGC	6 cut(s)	92, 309, 437, 1631, 1661, 1776
SspMI	CTAG	2 cut(s)	423, 672
StyD4I	CCNGG	1 cut(s)	727
StyI	CCWWGG	2 cut(s)	1174, 1665
TaaI	ACNGT	2 cut(s)	81, 1791
TaiI	ACGT	2 cut(s)	694, 1152
TaqI	TCGA	7 cut(s)	733, 940, 1351, 1360, 1697, 1732, 1768
TasI	AATT	5 cut(s)	185, 709, 1124, 1415, 1579
TatI	WGTACW	2 cut(s)	1250, 1609
TfiI	GAWTC	6 cut(s)	86, 415, 765, 859, 1239, 1740
Tru1I	TTAA	5 cut(s)	927, 1152, 1452, 1467, 1605
Tru9I	TTAA	5 cut(s)	927, 1152, 1452, 1467, 1605
TscAI	CASTG	2 cut(s)	1125, 1750
TseFI	GTSAC	1 cut(s)	991
TseI	GCWGC	6 cut(s)	467, 520, 1189, 1262, 1436, 1637
Tsp45I	GTSAC	1 cut(s)	991
TspGWI	ACGGA	3 cut(s)	131, 567, 1343
TspRI	CASTG	2 cut(s)	1125, 1750
Tth111I	GACNNNGTC	1 cut(s)	478
Van91I	CCANNNNNTGG	1 cut(s)	610
VpaK11BI	GGWCC	1 cut(s)	619
XapI	RAATTY	2 cut(s)	709, 1124
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1003
XhoI	CTCGAG	1 cut(s)	1359
XmiI	GTMKAC	2 cut(s)	733, 1556
XspI	CTAG	2 cut(s)	423, 672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.