Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0003249

Physical Location & Seq

Genomic Coordinates Forward (+)

22350966 .. 22354842

Gene Structure 3877 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom06g18710.9

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1674 bp

ATGACAGACCAAAACCTTCACGCGCCGCCGGAGGACGAGACGAAGAAGAAGAAGCGCAAGAGAAACAGAGCGAAGAAGCCGTCGGAATCGGCGGGTACGGAGCAACCGGAGCCCGAAAATACTAAGGGGGAACAGGCTAAGGGCGAGAGCGAGAAGAAAAGGAAGAAGGTGAAGAAATTGAAGGGAGAGGAACCGGAGGCTCAGAGCCCTGTGGAGGACAATGAGGAAGAAGAAGAGGAGGGGGAAGAAGCAATGGAGGAGGAGGAGGAGGAGGAGAGGAAAGTGAGAAGTGGCGGCGGAGGGACTGGGATTATGAGCACGGAGTCGTTTGCTTCGTTGAATTTGTCTGAGAACACTTACAAGGCCATTGAAGAAATGAAGTTTCAGTATATGACTCAGATTCAAGCTAGAGCAATCCCACCGCTCTTGATCGGGAAAGATGTCCTCGGAGCTGCAAGGACAGGGTCTGGTAAAACTCTTGCTTTTCTAATACCAGCTGTGGAGCTGCTATATAATACACATTTTGCTCCTCGTAACGGAATGGGTGTTGTTGTGATTTGCCCAACACGGGAACTTGCAATACAGACCCATGCTGTGGCAAAGGACCTTCTAAAGTATCACTCTCAGACTCTTGGCTTGGTAATTGGAGGTTCAGCTAGGAGAGGGGAAGCAGAGCGTATTGTAAAAGGAGTAAATTTATTGGTTGCAACCCCTGGTCGACTTCTTGACCATCTTCAGAATACCAAGGGATTCATATATAAGAACTTGAAGTGCCTCGTGATTGATGAAGCTGACAGGTTATTGGAAGCAAACTTTGAGGAGGAAATGAAACAGATTGTCAAGATTCTGCCAAAGGATAGGCAAACAGCTTTATTTTCAGCAACCCAAACCAAGAAGGTTGAAGATCTTGTTAAGTTGTCGTTTCGAAAAGATCAGAAACCTATCTACGTTGATGTGGACGAGGGGAGGTCAAAGGTCACCAATGAAGGGCTGGAACAAGGCTATTGTGTTGTGCCAAGTGCTAAGAGATTTCTACTCTTATATTCCTTCCTGATAAAGAATACGTCTAAGAAAGTTATGGTCTTCTTCTCGTCTTGTAACTCAGTGAAATTTCACTCTGAACTTCTTAGATACGTTAATGTGGATTGCTTTGATATCCATGGAAAGCAAAAGCAGCAGAAGAGGACAACTACTTTCTTTGACTTTTGCAAAGCAGAGAAAGGAATCTTACTTTGTACTGATGTTGCTGCTCGTGGACTAGATATTCCTGCTGTGGACTGGATTTTGCAGTTTGATCCTCCAGATGAACCGACGGAATATATCCATAGGGTTGGTCGAACAGCTCGAGGGGAAGGTGCAAAAGGAAAGGCACTACTTTTCCTCATTCCTGAAGAGTTGCAATTCATTCGCTATTTGAAGGCTGCAAATGTCCCCGTTAAACCATACGATTTTAATGAAAAGAAGCTGAAGAATGTGCAGTCTAAACTGGAGGAGATGGCGGTTGCAGCTTCGTTTTGCTTTTCTAATCCACCCAAGGTGCATTTGAACCTAGACAGCAACAAATCAAAGTTCAAGAAGAAGATGCGTAAAGAAGTAAGTCGAAATGGATTCAGTGAGAGCAATCCTTACGGCAATCGAAAAGGCGGAGACGATAGACAATTTGTAAGGCACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DUF4217 Helicase_C

Protein Analysis

558

Amino Acids

62.85

Weight (kDa)

8.95

Isoelectric Point (pI)

44.03

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003249)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18600
fragaria_vesca	FvH4_5g12870
malus_domestica	MD05G1177700.v1.1 MD06G1209500.v1.1 MD14G1220200.v1.1 MD15G1015500.v1.1
prunus_persica	Prupe.5G214700_v2.0.a1 Prupe.6G096100_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G096400_v2.0.a1 Prupe.6G097300_v2.0.a1
pyrus_communis	pycom05g16360 pycom05g16400 pycom05g16410 pycom06g18710 pycom14g18230 pycom15g01320
rosa_chinensis	RchiOBHm_Chr5g0083351 RchiOBHm_Chr5g0083361 RchiOBHm_Chr7g0184361
rosa_laevigata	RLG00000004982 RLG00000037030
rosa_multiflora	Rmu_sc0001444.1_g000013 Rmu_sc0001557.1_g000022 Rmu_sc0002245.1_g000045
rosa_roxburghii	Rroxscaffold_1G00001130 Rroxscaffold_3G00269690
rosa_samantha	Rh5AG533800 Rh5BG561600 Rh5CG583900 Rh5CG584000 Rh7AG080700 Rh7BG066500 Rh7CG066800 Rh7DG066500
rosa_wichuraiana	Rw5G049900 Rw7G005460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	595
AccBSI	CCGCTC	1 cut(s)	424
AccI	GTMKAC	1 cut(s)	718
AccII	CGCG	1 cut(s)	23
AciI	CCGC	7 cut(s)	26, 92, 294, 297, 422, 1499, 1644
AclWI	GGATC	1 cut(s)	1289
AcsI	RAATTY	3 cut(s)	340, 694, 1109
AcuI	CTGAAG	3 cut(s)	719, 1410, 1487
AfaI	GTAC	2 cut(s)	97, 1237
AfiI	CCNNNNNNNGG	5 cut(s)	214, 536, 568, 595, 987
AgsI	TTSAA	9 cut(s)	181, 340, 371, 404, 769, 902, 1417, 1546, 1573
AjnI	CCWGG	1 cut(s)	712
Alw21I	GWGCWC	1 cut(s)	320
Alw26I	GTCTC	2 cut(s)	32, 1641
AlwI	GGATC	1 cut(s)	1289
AlwNI	CAGNNNCTG	1 cut(s)	467
Ama87I	CYCGRG	1 cut(s)	1344
AoxI	GGCC	1 cut(s)	363
ApeKI	GCWGC	6 cut(s)	452, 505, 1174, 1247, 1421, 1505
ApoI	RAATTY	3 cut(s)	340, 694, 1109
ArsI	GACNNNNNNTTYG	2 cut(s)	65, 97
AspLEI	GCGC	2 cut(s)	25, 57
AspS9I	GGNCC	1 cut(s)	604
AsuHPI	GGTGA	2 cut(s)	181, 970
AsuII	TTCGAA	1 cut(s)	925
AvaI	CYCGRG	1 cut(s)	1344
AvaII	GGWCC	1 cut(s)	604
BanII	GRGCYC	2 cut(s)	114, 209
BauI	CACGAG	2 cut(s)	776, 1251
BbsI	GAAGAC	1 cut(s)	1075
Bbv12I	GWGCWC	1 cut(s)	320
BbvI	GCAGC	6 cut(s)	439, 492, 1186, 1234, 1408, 1517
BccI	CCATC	2 cut(s)	738, 1489
BceAI	ACGGC	2 cut(s)	64, 1645
BcgI	CGANNNNNNTGC	2 cut(s)	1388, 1422
BciT130I	CCWGG	1 cut(s)	714
BcoDI	GTCTC	2 cut(s)	32, 1641
BfaI	CTAG	5 cut(s)	408, 657, 1259, 1550, 1672
BglII	AGATCT	1 cut(s)	904
BisI	GCNGC	8 cut(s)	26, 295, 453, 506, 1175, 1248, 1422, 1506
BlsI	GCNGC	8 cut(s)	27, 296, 454, 507, 1176, 1249, 1423, 1507
Bme1390I	CCNGG	1 cut(s)	714
Bme18I	GGWCC	1 cut(s)	604
BmeT110I	CYCGRG	1 cut(s)	1344
BmgT120I	GGNCC	1 cut(s)	604
BmiI	GGNNCC	2 cut(s)	111, 192
BmrFI	CCNGG	1 cut(s)	714
BmrI	ACTGGG	1 cut(s)	315
BmsI	GCATC	1 cut(s)	1572
BmuI	ACTGGG	1 cut(s)	315
BpiI	GAAGAC	1 cut(s)	1075
BpmI	CTGGAG	2 cut(s)	1284, 1508
Bpu10I	CCTNAGC	1 cut(s)	138
Bpu14I	TTCGAA	1 cut(s)	925
BsaJI	CCNNGG	5 cut(s)	445, 712, 744, 1159, 1533
BsaWI	WCCGGW	2 cut(s)	106, 193
Bsc4I	CCNNNNNNNGG	5 cut(s)	214, 536, 568, 595, 987
Bse1I	ACTGG	3 cut(s)	310, 1283, 1491
Bse3DI	GCAATG	1 cut(s)	258
BseBI	CCWGG	1 cut(s)	714
BseDI	CCNNGG	5 cut(s)	445, 712, 744, 1159, 1533
BseLI	CCNNNNNNNGG	5 cut(s)	214, 536, 568, 595, 987
BseMI	GCAATG	1 cut(s)	258
BseMII	CTCAG	5 cut(s)	215, 339, 410, 638, 1116
BseNI	ACTGG	3 cut(s)	310, 1283, 1491
BseXI	GCAGC	6 cut(s)	439, 492, 1186, 1234, 1408, 1517
BsgI	GTGCAG	1 cut(s)	1496
Bsh1236I	CGCG	1 cut(s)	23
BshFI	GGCC	1 cut(s)	365
BsiHKAI	GWGCWC	1 cut(s)	320
BsiHKCI	CYCGRG	1 cut(s)	1344
BsiSI	CCGG	3 cut(s)	29, 107, 194
BslFI	GGGAC	2 cut(s)	316, 1415
BslI	CCNNNNNNNGG	5 cut(s)	214, 536, 568, 595, 987
BsmAI	GTCTC	2 cut(s)	32, 1641
BsmBI	CGTCTC	2 cut(s)	32, 1641
BsmFI	GGGAC	2 cut(s)	316, 1415
BsnI	GGCC	1 cut(s)	365
BsoBI	CYCGRG	1 cut(s)	1344
Bsp119I	TTCGAA	1 cut(s)	925
Bsp1286I	GDGCHC	3 cut(s)	114, 209, 320
Bsp143I	GATC	4 cut(s)	429, 904, 931, 1294
Bsp19I	CCATGG	1 cut(s)	1159
BspACI	CCGC	7 cut(s)	26, 92, 294, 297, 422, 1499, 1644
BspANI	GGCC	1 cut(s)	365
BspCNI	CTCAG	5 cut(s)	214, 340, 409, 637, 1115
BspFNI	CGCG	1 cut(s)	23
BspLI	GGNNCC	2 cut(s)	111, 192
BspPI	GGATC	1 cut(s)	1289
BspT104I	TTCGAA	1 cut(s)	925
BsrBI	CCGCTC	1 cut(s)	424
BsrDI	GCAATG	1 cut(s)	258
BsrI	ACTGG	3 cut(s)	310, 1283, 1491
BssECI	CCNNGG	5 cut(s)	445, 712, 744, 1159, 1533
BssMI	GATC	4 cut(s)	429, 904, 931, 1294
BssSI	CACGAG	2 cut(s)	776, 1251
BssT1I	CCWWGG	3 cut(s)	744, 1159, 1533
Bst2BI	CACGAG	2 cut(s)	776, 1251
Bst2UI	CCWGG	1 cut(s)	714
Bst6I	CTCTTC	3 cut(s)	228, 1175, 1386
BstBI	TTCGAA	1 cut(s)	925
BstDSI	CCRYGG	1 cut(s)	1159
BstEII	GGTNACC	1 cut(s)	976
BstFNI	CGCG	1 cut(s)	23
BstHHI	GCGC	2 cut(s)	25, 57
BstKTI	GATC	4 cut(s)	432, 907, 934, 1297
BstMAI	GTCTC	2 cut(s)	32, 1641
BstMBI	GATC	4 cut(s)	429, 904, 931, 1294
BstMWI	GCNNNNNNNGC	3 cut(s)	109, 1174, 1505
BstNI	CCWGG	1 cut(s)	714
BstPI	GGTNACC	1 cut(s)	976
BstSCI	CCNGG	1 cut(s)	712
BstUI	CGCG	1 cut(s)	23
BstV1I	GCAGC	6 cut(s)	439, 492, 1186, 1234, 1408, 1517
BstV2I	GAAGAC	1 cut(s)	1075
BstX2I	RGATCY	1 cut(s)	904
BstXI	CCANNNNNNTGG	1 cut(s)	1331
BstYI	RGATCY	1 cut(s)	904
BsuRI	GGCC	1 cut(s)	365
BtgI	CCRYGG	1 cut(s)	1159
BtsIMutI	CAGTG	2 cut(s)	1110, 1618
CaiI	CAGNNNCTG	1 cut(s)	467
CfoI	GCGC	2 cut(s)	25, 57
Cfr13I	GGNCC	1 cut(s)	604
Csp6I	GTAC	2 cut(s)	96, 1236
CspCI	CAANNNNNGTGG	2 cut(s)	408, 443
CviAII	CATG	2 cut(s)	590, 1160
CviQI	GTAC	2 cut(s)	96, 1236
DpnI	GATC	4 cut(s)	431, 906, 933, 1296
DpnII	GATC	4 cut(s)	429, 904, 931, 1294
Eam1104I	CTCTTC	3 cut(s)	228, 1175, 1386
EarI	CTCTTC	3 cut(s)	228, 1175, 1386
EciI	GGCGGA	2 cut(s)	312, 1659
Eco130I	CCWWGG	3 cut(s)	744, 1159, 1533
Eco24I	GRGCYC	2 cut(s)	114, 209
Eco32I	GATATC	1 cut(s)	1156
Eco47I	GGWCC	1 cut(s)	604
Eco57I	CTGAAG	3 cut(s)	719, 1410, 1487
Eco88I	CYCGRG	1 cut(s)	1344
Eco91I	GGTNACC	1 cut(s)	976
EcoO109I	RGGNCCY	1 cut(s)	604
EcoO65I	GGTNACC	1 cut(s)	976
EcoRII	CCWGG	1 cut(s)	712
EcoRV	GATATC	1 cut(s)	1156
EcoT14I	CCWWGG	3 cut(s)	744, 1159, 1533
EcoT38I	GRGCYC	2 cut(s)	114, 209
ErhI	CCWWGG	3 cut(s)	744, 1159, 1533
Esp3I	CGTCTC	2 cut(s)	32, 1641
FaeI	CATG	2 cut(s)	593, 1163
FaqI	GGGAC	2 cut(s)	316, 1415
FatI	CATG	2 cut(s)	589, 1159
FauI	CCCGC	1 cut(s)	85
FblI	GTMKAC	1 cut(s)	718
Fnu4HI	GCNGC	8 cut(s)	26, 295, 453, 506, 1175, 1248, 1422, 1506
FriOI	GRGCYC	2 cut(s)	114, 209
Fsp4HI	GCNGC	8 cut(s)	26, 295, 453, 506, 1175, 1248, 1422, 1506
FspBI	CTAG	5 cut(s)	408, 657, 1259, 1550, 1672
GlaI	GCGC	2 cut(s)	24, 56
GluI	GCNGC	8 cut(s)	26, 295, 453, 506, 1175, 1248, 1422, 1506
GsuI	CTGGAG	2 cut(s)	1284, 1508
HaeIII	GGCC	1 cut(s)	365
HapII	CCGG	3 cut(s)	29, 107, 194
HhaI	GCGC	2 cut(s)	25, 57
Hin1II	CATG	2 cut(s)	593, 1163
Hin6I	GCGC	2 cut(s)	23, 55
HinP1I	GCGC	2 cut(s)	23, 55
HincII	GTYRAC	1 cut(s)	719
HindII	GTYRAC	1 cut(s)	719
HinfI	GANTC	9 cut(s)	86, 323, 394, 400, 628, 750, 844, 1224, 1608
HpaII	CCGG	3 cut(s)	29, 107, 194
HphI	GGTGA	2 cut(s)	181, 970
Hpy166II	GTNNAC	4 cut(s)	719, 958, 1256, 1276
Hpy188I	TCNGA	9 cut(s)	85, 204, 349, 399, 449, 627, 738, 936, 1120
Hpy188III	TCNNGA	9 cut(s)	427, 433, 725, 778, 841, 1051, 1301, 1388, 1573
Hpy8I	GTNNAC	4 cut(s)	719, 958, 1256, 1276
Hpy99I	CGWCG	2 cut(s)	85, 1315
HpyAV	CCTTC	9 cut(s)	26, 160, 175, 617, 889, 980, 1057, 1346, 1411
HpyCH4IV	ACGT	3 cut(s)	948, 1064, 1134
HpyF10VI	GCNNNNNNNGC	3 cut(s)	109, 1174, 1505
HpySE526I	ACGT	3 cut(s)	948, 1064, 1134
Hsp92II	CATG	2 cut(s)	593, 1163
HspAI	GCGC	2 cut(s)	23, 55
Kzo9I	GATC	4 cut(s)	429, 904, 931, 1294
LmnI	GCTCC	5 cut(s)	100, 109, 449, 502, 532
Lsp1109I	GCAGC	6 cut(s)	439, 492, 1186, 1234, 1408, 1517
LweI	GCATC	1 cut(s)	1572
MaeI	CTAG	5 cut(s)	408, 657, 1259, 1550, 1672
MaeII	ACGT	3 cut(s)	948, 1064, 1134
MaeIII	GTNAC	3 cut(s)	533, 976, 1097
MalI	GATC	4 cut(s)	431, 906, 933, 1296
MbiI	CCGCTC	1 cut(s)	424
MboI	GATC	4 cut(s)	429, 904, 931, 1294
MflI	RGATCY	1 cut(s)	904
MhlI	GDGCHC	3 cut(s)	114, 209, 320
MluCI	AATT	7 cut(s)	176, 340, 642, 694, 1109, 1400, 1658
MlyI	GAGTC	3 cut(s)	332, 388, 622
MmeI	TCCRAC	1 cut(s)	63
MseI	TTAA	4 cut(s)	912, 1137, 1437, 1452
MspA1I	CMGCKG	1 cut(s)	497
MspI	CCGG	3 cut(s)	29, 107, 194
MspR9I	CCNGG	1 cut(s)	714
MvaI	CCWGG	1 cut(s)	714
MvnI	CGCG	1 cut(s)	23
MwoI	GCNNNNNNNGC	3 cut(s)	109, 1174, 1505
NcoI	CCATGG	1 cut(s)	1159
NdeII	GATC	4 cut(s)	429, 904, 931, 1294
NlaIII	CATG	2 cut(s)	593, 1163
NlaIV	GGNNCC	2 cut(s)	111, 192
NmuCI	GTSAC	1 cut(s)	976
NspV	TTCGAA	1 cut(s)	925
PaeR7I	CTCGAG	1 cut(s)	1344
PcsI	WCGNNNNNNNCGW	2 cut(s)	332, 1342
PfeI	GAWTC	6 cut(s)	86, 400, 750, 844, 1224, 1608
PflFI	GACNNNGTC	1 cut(s)	463
PflMI	CCANNNNNTGG	1 cut(s)	595
PkrI	GCNGC	8 cut(s)	27, 296, 454, 507, 1176, 1249, 1423, 1507
PleI	GAGTC	3 cut(s)	331, 388, 622
PpsI	GAGTC	3 cut(s)	331, 388, 622
PpuMI	RGGWCCY	1 cut(s)	604
Psp5II	RGGWCCY	1 cut(s)	604
Psp6I	CCWGG	1 cut(s)	712
PspEI	GGTNACC	1 cut(s)	976
PspGI	CCWGG	1 cut(s)	712
PspN4I	GGNNCC	2 cut(s)	111, 192
PspPI	GGNCC	1 cut(s)	604
PspPPI	RGGWCCY	1 cut(s)	604
PspXI	VCTCGAGB	1 cut(s)	1344
PsrI	GAACNNNNNNTAC	2 cut(s)	564, 596
PstNI	CAGNNNCTG	1 cut(s)	467
PsuI	RGATCY	1 cut(s)	904
PsyI	GACNNNGTC	1 cut(s)	463
PvuII	CAGCTG	1 cut(s)	497
RsaI	GTAC	2 cut(s)	97, 1237
RsaNI	GTAC	2 cut(s)	96, 1236
SalI	GTCGAC	1 cut(s)	717
SaqAI	TTAA	4 cut(s)	912, 1137, 1437, 1452
SatI	GCNGC	8 cut(s)	26, 295, 453, 506, 1175, 1248, 1422, 1506
Sau3AI	GATC	4 cut(s)	429, 904, 931, 1294
Sau96I	GGNCC	1 cut(s)	604
SchI	GAGTC	3 cut(s)	332, 388, 622
ScrFI	CCNGG	1 cut(s)	714
SduI	GDGCHC	3 cut(s)	114, 209, 320
SfaNI	GCATC	1 cut(s)	1572
Sfr274I	CTCGAG	1 cut(s)	1344
SfuI	TTCGAA	1 cut(s)	925
SinI	GGWCC	1 cut(s)	604
SlaI	CTCGAG	1 cut(s)	1344
SmlI	CTYRAG	1 cut(s)	1344
SmoI	CTYRAG	1 cut(s)	1344
Sse9I	AATT	7 cut(s)	176, 340, 642, 694, 1109, 1400, 1658
SsiI	CCGC	7 cut(s)	26, 92, 294, 297, 422, 1499, 1644
SspMI	CTAG	5 cut(s)	408, 657, 1259, 1550, 1672
StyD4I	CCNGG	1 cut(s)	712
StyI	CCWWGG	3 cut(s)	744, 1159, 1533
TaiI	ACGT	3 cut(s)	951, 1067, 1137
TaqI	TCGA	6 cut(s)	718, 925, 1336, 1345, 1600, 1636
TasI	AATT	7 cut(s)	176, 340, 642, 694, 1109, 1400, 1658
TatI	WGTACW	1 cut(s)	1235
TauI	GCSGC	2 cut(s)	28, 297
TfiI	GAWTC	6 cut(s)	86, 400, 750, 844, 1224, 1608
Tru1I	TTAA	4 cut(s)	912, 1137, 1437, 1452
Tru9I	TTAA	4 cut(s)	912, 1137, 1437, 1452
TscAI	CASTG	2 cut(s)	1110, 1618
TseFI	GTSAC	1 cut(s)	976
TseI	GCWGC	6 cut(s)	452, 505, 1174, 1247, 1421, 1505
Tsp45I	GTSAC	1 cut(s)	976
TspDTI	ATGAA	8 cut(s)	392, 742, 801, 842, 999, 1320, 1393, 1470
TspGWI	ACGGA	4 cut(s)	113, 335, 552, 1328
TspRI	CASTG	2 cut(s)	1110, 1618
Tth111I	GACNNNGTC	1 cut(s)	463
Van91I	CCANNNNNTGG	1 cut(s)	595
VpaK11BI	GGWCC	1 cut(s)	604
XapI	RAATTY	3 cut(s)	340, 694, 1109
XcmI	CCANNNNNNNNNTGG	1 cut(s)	988
XhoI	CTCGAG	1 cut(s)	1344
XmiI	GTMKAC	1 cut(s)	718
XspI	CTAG	5 cut(s)	408, 657, 1259, 1550, 1672

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.