MD07G1120400.v1.1

tRNA 2'-phosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002921
Physical Location & Seq
Reverse (-)
15033604 .. 15036419
2816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1120400.v1.1.491

Sequence Viewer

Length: 771 bp
ATGGACAACAACAATCGCAACGTCAGCAGCAGCAGTACCTCCGGAAGTGGAGGAGGAGGAAGACATTGGGAGGTGAAAAATGATAGAGAACGATCGAGGGGCCGCGGTGGCGGCGGAGTTGGTGGCGCTGGAAAAGATAAAATTGATGCACTTGGTAGATTATTGACGCGTATATTGCGCCATATGGCGGGTGAGTTGAGTTTGAATATGCGAAGCGATGGATATGTGAAGGTTAATGATCTGTTAAAGCTAAACATAAAGACATTTGCTAATATTCCAGTGAGATTACATACTATTGATGAGATTAAGGAGGCTGTCAGAAAGGATGATAAGCAGCGCTTTGGCCTCTTGGAAGAAAATGGGGAACTTTTGATACGTGCAAACCAAGGCCACACAACAAAGATAGTTGAATCTGAAAGATTGTTAACACCAATCATTTCATCCGAAGAAGTTCCAGTTTGCGTGCATGGGACCTATAGGAGGAATTTGGAATCGATATTAAGGTCCGGTCTAAAAAGCATGAAAAGATTACATGCTCACTTCTCACGTGGCTTGCCGACAGATGGGCAAGTGATTAGCGGTATGAGACGCGATGTCAATGTTTTGATCTATCTCGATGTTAAAAAAGCTTTGGAAGAGGGAATGAAGTTTTACATTTCAGAGAACAAGGTGATCTTGACTGAAGGTTTCGAGGGTGTTGTTCCAGTCAAGTACTTTGAGAAAATAGAATCATGGCCCGATAGAACGCCAATACCTTTCAGAAGAATCTGA

Protein Analysis

257

Amino Acids

28.76

Weight (kDa)

9.73

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTS_2-RNA PF01885 50 - 229 4.2e-57 RNA 2'-phosphotransferase, Tpt1 / KptA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 105, 169, 591
AccIII TCCGGA 1 cut(s) 41
AciI CCGC 6 cut(s) 103, 105, 111, 114, 188, 579
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 1 cut(s) 702
AcvI CACGTG 1 cut(s) 548
AfaI GTAC 2 cut(s) 37, 713
AfeI AGCGCT 1 cut(s) 338
AfiI CCNNNNNNNGG 3 cut(s) 187, 480, 563
AflIII ACRYGT 1 cut(s) 167
AgsI TTSAA 2 cut(s) 205, 410
AhdI GACNNNNNGTC 1 cut(s) 593
AluBI AGCT 2 cut(s) 250, 629
AluI AGCT 2 cut(s) 250, 629
Alw26I GTCTC 1 cut(s) 580
Aor13HI TCCGGA 1 cut(s) 41
Aor51HI AGCGCT 1 cut(s) 338
AoxI GGCC 4 cut(s) 100, 343, 388, 734
ApeKI GCWGC 3 cut(s) 27, 30, 334
ApoI RAATTY 1 cut(s) 484
Asp700I GAANNNNTTC 1 cut(s) 450
AspLEI GCGC 3 cut(s) 128, 180, 339
AspS9I GGNCC 4 cut(s) 100, 471, 504, 735
AsuHPI GGTGA 3 cut(s) 85, 203, 682
AvaII GGWCC 2 cut(s) 471, 504
BbrPI CACGTG 1 cut(s) 548
BbsI GAAGAC 1 cut(s) 67
BbvI GCAGC 3 cut(s) 39, 42, 346
BccI CCATC 2 cut(s) 212, 557
BcoDI GTCTC 1 cut(s) 580
BfmI CTRYAG 1 cut(s) 475
BfoI RGCGCY 2 cut(s) 129, 340
BglI GCCNNNNNGGC 1 cut(s) 108
BisI GCNGC 5 cut(s) 28, 31, 103, 112, 335
BlsI GCNGC 5 cut(s) 29, 32, 104, 113, 336
BmcAI AGTACT 1 cut(s) 713
Bme18I GGWCC 2 cut(s) 471, 504
BmeRI GACNNNNNGTC 1 cut(s) 593
BmgT120I GGNCC 4 cut(s) 100, 471, 504, 735
BmiI GGNNCC 2 cut(s) 101, 472
BmsI GCATC 1 cut(s) 136
BpiI GAAGAC 1 cut(s) 67
Bsa29I ATCGAT 1 cut(s) 494
BsaAI YACGTR 2 cut(s) 377, 548
BsaJI CCNNGG 2 cut(s) 103, 385
BsaWI WCCGGW 2 cut(s) 41, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 480, 563
Bse1I ACTGG 3 cut(s) 278, 455, 704
BseAI TCCGGA 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 103, 385
BseGI GGATG 2 cut(s) 331, 440
BseLI CCNNNNNNNGG 3 cut(s) 187, 480, 563
BseNI ACTGG 3 cut(s) 278, 455, 704
BseRI GAGGAG 2 cut(s) 66, 69
BseXI GCAGC 3 cut(s) 39, 42, 346
Bsh1236I CGCG 3 cut(s) 105, 169, 591
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 4 cut(s) 102, 345, 390, 736
BshVI ATCGAT 1 cut(s) 494
BsiEI CGRYCG 1 cut(s) 95
BsiSI CCGG 2 cut(s) 42, 507
BslFI GGGAC 1 cut(s) 484
BslI CCNNNNNNNGG 3 cut(s) 187, 480, 563
BsmAI GTCTC 1 cut(s) 580
BsmBI CGTCTC 1 cut(s) 580
BsmFI GGGAC 1 cut(s) 484
BsnI GGCC 4 cut(s) 102, 345, 390, 736
Bsp13I TCCGGA 1 cut(s) 41
Bsp143I GATC 4 cut(s) 92, 238, 606, 672
BspACI CCGC 6 cut(s) 103, 105, 111, 114, 188, 579
BspANI GGCC 4 cut(s) 102, 345, 390, 736
BspDI ATCGAT 1 cut(s) 494
BspEI TCCGGA 1 cut(s) 41
BspFNI CGCG 3 cut(s) 105, 169, 591
BspLI GGNNCC 2 cut(s) 101, 472
BsrI ACTGG 3 cut(s) 278, 455, 704
BssECI CCNNGG 2 cut(s) 103, 385
BssMI GATC 4 cut(s) 92, 238, 606, 672
BssT1I CCWWGG 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 630
BstBAI YACGTR 2 cut(s) 377, 548
BstC8I GCNNGC 2 cut(s) 464, 554
BstDSI CCRYGG 1 cut(s) 103
BstENI CCTNNNNNAGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 331, 440
BstFNI CGCG 3 cut(s) 105, 169, 591
BstH2I RGCGCY 2 cut(s) 129, 340
BstHHI GCGC 3 cut(s) 128, 180, 339
BstKTI GATC 4 cut(s) 95, 241, 609, 675
BstMAI GTCTC 1 cut(s) 580
BstMBI GATC 4 cut(s) 92, 238, 606, 672
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 4 cut(s) 24, 108, 111, 175
BstNSI RCATGY 1 cut(s) 536
BstSFI CTRYAG 1 cut(s) 475
BstUI CGCG 3 cut(s) 105, 169, 591
BstV1I GCAGC 3 cut(s) 39, 42, 346
BstV2I GAAGAC 1 cut(s) 67
Bsu15I ATCGAT 1 cut(s) 494
BsuRI GGCC 4 cut(s) 102, 345, 390, 736
BsuTUI ATCGAT 1 cut(s) 494
BtgI CCRYGG 1 cut(s) 103
BtgZI GCGATG 2 cut(s) 231, 606
BtsCI GGATG 2 cut(s) 331, 440
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 464, 554
CfoI GCGC 3 cut(s) 128, 180, 339
Cfr13I GGNCC 4 cut(s) 100, 471, 504, 735
Cfr42I CCGCGG 1 cut(s) 106
ClaI ATCGAT 1 cut(s) 494
CseI GACGC 2 cut(s) 175, 597
Csp6I GTAC 2 cut(s) 36, 712
CviAII CATG 4 cut(s) 467, 520, 533, 732
CviJI RGCY 8 cut(s) 102, 250, 314, 345, 390, 552, 629, 736
CviKI_1 RGCY 8 cut(s) 102, 250, 314, 345, 390, 552, 629, 736
CviQI GTAC 2 cut(s) 36, 712
DpnI GATC 4 cut(s) 94, 240, 608, 674
DpnII GATC 4 cut(s) 92, 238, 606, 672
DriI GACNNNNNGTC 1 cut(s) 593
Eam1104I CTCTTC 1 cut(s) 630
Eam1105I GACNNNNNGTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 385
Eco47I GGWCC 2 cut(s) 471, 504
Eco47III AGCGCT 1 cut(s) 338
Eco57I CTGAAG 1 cut(s) 702
Eco72I CACGTG 1 cut(s) 548
EcoNI CCTNNNNNAGG 1 cut(s) 478
EcoO109I RGGNCCY 1 cut(s) 471
EcoT14I CCWWGG 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 580
FaeI CATG 4 cut(s) 470, 523, 536, 735
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 659, 691
FaqI GGGAC 1 cut(s) 484
FatI CATG 4 cut(s) 466, 519, 532, 731
FauI CCCGC 1 cut(s) 181
FauNDI CATATG 1 cut(s) 183
Fnu4HI GCNGC 5 cut(s) 28, 31, 103, 112, 335
FokI GGATG 2 cut(s) 338, 427
Fsp4HI GCNGC 5 cut(s) 28, 31, 103, 112, 335
GlaI GCGC 3 cut(s) 127, 179, 338
GluI GCNGC 5 cut(s) 28, 31, 103, 112, 335
HaeII RGCGCY 2 cut(s) 129, 340
HaeIII GGCC 4 cut(s) 102, 345, 390, 736
HapII CCGG 2 cut(s) 42, 507
HgaI GACGC 2 cut(s) 175, 597
HhaI GCGC 3 cut(s) 128, 180, 339
Hin1II CATG 4 cut(s) 470, 523, 536, 735
Hin6I GCGC 3 cut(s) 126, 178, 337
HinP1I GCGC 3 cut(s) 126, 178, 337
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 4 cut(s) 410, 491, 728, 765
HpaI GTTAAC 1 cut(s) 426
HpaII CCGG 2 cut(s) 42, 507
HphI GGTGA 3 cut(s) 85, 203, 682
Hpy166II GTNNAC 1 cut(s) 426
Hpy188I TCNGA 6 cut(s) 320, 415, 445, 661, 761, 770
Hpy188III TCNNGA 3 cut(s) 42, 614, 676
Hpy8I GTNNAC 1 cut(s) 426
HpyAV CCTTC 2 cut(s) 223, 677
HpyCH4IV ACGT 3 cut(s) 21, 376, 547
HpyCH4V TGCA 3 cut(s) 149, 380, 466
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 108, 111, 175
HpySE526I ACGT 3 cut(s) 21, 376, 547
Hsp92II CATG 4 cut(s) 470, 523, 536, 735
HspAI GCGC 3 cut(s) 126, 178, 337
Kpn2I TCCGGA 1 cut(s) 41
KspAI GTTAAC 1 cut(s) 426
KspI CCGCGG 1 cut(s) 106
Kzo9I GATC 4 cut(s) 92, 238, 606, 672
LpnPI CCDG 6 cut(s) 55, 114, 291, 468, 520, 717
Lsp1109I GCAGC 3 cut(s) 39, 42, 346
LweI GCATC 1 cut(s) 136
MaeII ACGT 3 cut(s) 21, 376, 547
MalI GATC 4 cut(s) 94, 240, 608, 674
MboI GATC 4 cut(s) 92, 238, 606, 672
MboII GAAGA 4 cut(s) 72, 365, 458, 647
MluCI AATT 2 cut(s) 141, 484
MluI ACGCGT 1 cut(s) 167
MroI TCCGGA 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 450
MseI TTAA 6 cut(s) 234, 245, 306, 425, 500, 621
MspA1I CMGCKG 1 cut(s) 105
MspI CCGG 2 cut(s) 42, 507
MvnI CGCG 3 cut(s) 105, 169, 591
MwoI GCNNNNNNNGC 4 cut(s) 24, 108, 111, 175
NdeI CATATG 1 cut(s) 183
NdeII GATC 4 cut(s) 92, 238, 606, 672
NlaIII CATG 4 cut(s) 470, 523, 536, 735
NlaIV GGNNCC 2 cut(s) 101, 472
NspI RCATGY 1 cut(s) 536
PdmI GAANNNNTTC 1 cut(s) 450
PfeI GAWTC 4 cut(s) 410, 491, 728, 765
PkrI GCNGC 5 cut(s) 29, 32, 104, 113, 336
Ple19I CGATCG 1 cut(s) 95
PmaCI CACGTG 1 cut(s) 548
PmlI CACGTG 1 cut(s) 548
Ppu21I YACGTR 2 cut(s) 377, 548
PpuMI RGGWCCY 1 cut(s) 471
Psp5II RGGWCCY 1 cut(s) 471
PspCI CACGTG 1 cut(s) 548
PspN4I GGNNCC 2 cut(s) 101, 472
PspPI GGNCC 4 cut(s) 100, 471, 504, 735
PspPPI RGGWCCY 1 cut(s) 471
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PvuI CGATCG 1 cut(s) 95
RsaI GTAC 2 cut(s) 37, 713
RsaNI GTAC 2 cut(s) 36, 712
SacII CCGCGG 1 cut(s) 106
SaqAI TTAA 6 cut(s) 234, 245, 306, 425, 500, 621
SatI GCNGC 5 cut(s) 28, 31, 103, 112, 335
Sau3AI GATC 4 cut(s) 92, 238, 606, 672
Sau96I GGNCC 4 cut(s) 100, 471, 504, 735
ScaI AGTACT 1 cut(s) 713
SfaNI GCATC 1 cut(s) 136
SfcI CTRYAG 1 cut(s) 475
Sfr303I CCGCGG 1 cut(s) 106
SgrBI CCGCGG 1 cut(s) 106
SinI GGWCC 2 cut(s) 471, 504
Sse9I AATT 2 cut(s) 141, 484
SsiI CCGC 6 cut(s) 103, 105, 111, 114, 188, 579
SspI AATATT 1 cut(s) 274
StyI CCWWGG 1 cut(s) 385
TaiI ACGT 3 cut(s) 24, 379, 550
TaqI TCGA 4 cut(s) 95, 494, 615, 690
TasI AATT 2 cut(s) 141, 484
TatI WGTACW 1 cut(s) 711
TauI GCSGC 2 cut(s) 105, 114
TfiI GAWTC 4 cut(s) 410, 491, 728, 765
Tru1I TTAA 6 cut(s) 234, 245, 306, 425, 500, 621
Tru9I TTAA 6 cut(s) 234, 245, 306, 425, 500, 621
TscAI CASTG 1 cut(s) 285
TseI GCWGC 3 cut(s) 27, 30, 334
TspDTI ATGAA 3 cut(s) 429, 536, 659
TspRI CASTG 1 cut(s) 285
VpaK11BI GGWCC 2 cut(s) 471, 504
XagI CCTNNNNNAGG 1 cut(s) 478
XapI RAATTY 1 cut(s) 484
XceI RCATGY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 450
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.