RLG00000013131

tRNA 2'-phosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002921
Physical Location & Seq
Reverse (-)
26621837 .. 26623761
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013131

Sequence Viewer

Length: 510 bp
ATGTCTATAGCTGCTTCTTCTTGCCAGATCTACGAGTCGGAAAGAGAGGGTTTGGGCTCTATCGTCGCCACTCCTGCAACCATGCGAAAGATGAGTCGGGCATCGCTCACGCGTTTGGGAAGAGGAGGAGGTTGGGGGGTGAAAGATGAGGGAGAGAAATCAAAAGGTCGCGGAGGAGGAAGGGGTCGTCGTGGTGGCGGCGGTGGCTCTGGCAAAGATAGAGTTGATGCACTCGGTATATTATCGACACATATATTGCGCCATATGGCTTCTGAGTTGAATTTGGATATGCGAAGTGATGAATTTGTGAAGGTTCATGATCTTTTAGAGCTAAACTTGAAGACATTTGCTAATATTCCATTGAGCTTGTATACTGTTGATGTGATTAAGGAGGCTGTCAGAAAGGATAATAAGCAACGTTTCAGCCTCTTGAAAGAAAATGGGGAACTTTTGGTATGTGTAAACCAAGGCCACTCAACAACGGTAACTATCCTCGTTTATCTTGCATAG

Protein Analysis

170

Amino Acids

18.29

Weight (kDa)

9.51

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTS_2-RNA PF01885 84 - 162 1.7e-15 RNA 2'-phosphotransferase, Tpt1 / KptA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 371
AccII CGCG 2 cut(s) 112, 171
AciI CCGC 3 cut(s) 171, 198, 201
AclI AACGTT 1 cut(s) 418
AcsI RAATTY 2 cut(s) 280, 302
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 3 cut(s) 280, 340, 433
AluBI AGCT 3 cut(s) 11, 331, 366
AluI AGCT 3 cut(s) 11, 331, 366
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 2 cut(s) 280, 302
ArsI GACNNNNNNTTYG 2 cut(s) 79, 111
AspLEI GCGC 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 151
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 1 cut(s) 347
BfmI CTRYAG 1 cut(s) 6
BglII AGATCT 1 cut(s) 27
BisI GCNGC 2 cut(s) 12, 199
BlsI GCNGC 2 cut(s) 13, 200
BmsI GCATC 2 cut(s) 110, 217
BpiI GAAGAC 1 cut(s) 347
BsaJI CCNNGG 1 cut(s) 466
BseDI CCNNGG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 264
BseRI GAGGAG 3 cut(s) 138, 141, 189
Bsh1236I CGCG 2 cut(s) 112, 171
BshFI GGCC 1 cut(s) 471
BsnI GGCC 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 59
Bsp143I GATC 2 cut(s) 27, 319
BspACI CCGC 3 cut(s) 171, 198, 201
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 2 cut(s) 112, 171
BspHI TCATGA 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 2 cut(s) 27, 319
BssNAI GTATAC 1 cut(s) 372
BssT1I CCWWGG 1 cut(s) 466
Bst1107I GTATAC 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 376, 484
Bst6I CTCTTC 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 273
BstFNI CGCG 2 cut(s) 112, 171
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 2 cut(s) 30, 322
BstMBI GATC 2 cut(s) 27, 319
BstMWI GCNNNNNNNGC 2 cut(s) 74, 204
BstSFI CTRYAG 1 cut(s) 6
BstUI CGCG 2 cut(s) 112, 171
BstV2I GAAGAC 1 cut(s) 347
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BstZ17I GTATAC 1 cut(s) 372
BsuRI GGCC 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 87
CciI TCATGA 1 cut(s) 316
CfoI GCGC 1 cut(s) 261
CviAII CATG 2 cut(s) 82, 317
CviJI RGCY 9 cut(s) 11, 57, 207, 269, 331, 366, 395, 426, 471
CviKI_1 RGCY 9 cut(s) 11, 57, 207, 269, 331, 366, 395, 426, 471
DdeI CTNAG 1 cut(s) 273
DpnI GATC 2 cut(s) 29, 321
DpnII GATC 2 cut(s) 27, 319
Eam1104I CTCTTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 1 cut(s) 466
FaeI CATG 2 cut(s) 85, 320
FatI CATG 2 cut(s) 81, 316
FauNDI CATATG 1 cut(s) 264
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 2 cut(s) 12, 199
FriOI GRGCYC 1 cut(s) 59
Fsp4HI GCNGC 2 cut(s) 12, 199
GlaI GCGC 1 cut(s) 260
GluI GCNGC 2 cut(s) 12, 199
HaeIII GGCC 1 cut(s) 471
HhaI GCGC 1 cut(s) 261
Hin1II CATG 2 cut(s) 85, 320
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HinfI GANTC 2 cut(s) 35, 94
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 2 cut(s) 372, 463
Hpy188I TCNGA 3 cut(s) 40, 274, 401
Hpy188III TCNNGA 2 cut(s) 317, 430
Hpy8I GTNNAC 2 cut(s) 372, 463
Hpy99I CGWCG 2 cut(s) 68, 192
HpyAV CCTTC 2 cut(s) 174, 304
HpyCH4III ACNGT 2 cut(s) 376, 484
HpyCH4IV ACGT 1 cut(s) 418
HpyCH4V TGCA 3 cut(s) 77, 230, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 204
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 1 cut(s) 418
Hsp92II CATG 2 cut(s) 85, 320
HspAI GCGC 1 cut(s) 259
Kzo9I GATC 2 cut(s) 27, 319
LpnPI CCDG 3 cut(s) 38, 87, 195
LweI GCATC 2 cut(s) 110, 217
MaeII ACGT 1 cut(s) 418
MaeIII GTNAC 1 cut(s) 484
MalI GATC 2 cut(s) 29, 321
MboI GATC 2 cut(s) 27, 319
MboII GAAGA 3 cut(s) 9, 132, 352
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 2 cut(s) 280, 302
MluI ACGCGT 1 cut(s) 110
MlyI GAGTC 2 cut(s) 44, 103
MmeI TCCRAC 1 cut(s) 18
MseI TTAA 1 cut(s) 387
MvnI CGCG 2 cut(s) 112, 171
MwoI GCNNNNNNNGC 2 cut(s) 74, 204
NdeI CATATG 1 cut(s) 264
NdeII GATC 2 cut(s) 27, 319
NlaIII CATG 2 cut(s) 85, 320
PagI TCATGA 1 cut(s) 316
PkrI GCNGC 2 cut(s) 13, 200
PleI GAGTC 2 cut(s) 43, 102
PpsI GAGTC 2 cut(s) 43, 102
Psp1406I AACGTT 1 cut(s) 418
PsuI RGATCY 1 cut(s) 27
SaqAI TTAA 1 cut(s) 387
SatI GCNGC 2 cut(s) 12, 199
Sau3AI GATC 2 cut(s) 27, 319
SchI GAGTC 2 cut(s) 44, 103
SduI GDGCHC 1 cut(s) 59
SetI ASST 7 cut(s) 13, 133, 169, 315, 333, 368, 421
SfaNI GCATC 2 cut(s) 110, 217
SfcI CTRYAG 1 cut(s) 6
Sse9I AATT 2 cut(s) 280, 302
SsiI CCGC 3 cut(s) 171, 198, 201
SspI AATATT 1 cut(s) 355
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 2 cut(s) 376, 484
TaiI ACGT 1 cut(s) 421
TaqI TCGA 1 cut(s) 245
TasI AATT 2 cut(s) 280, 302
TauI GCSGC 1 cut(s) 201
Tru1I TTAA 1 cut(s) 387
Tru9I TTAA 1 cut(s) 387
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 305, 315
XapI RAATTY 2 cut(s) 280, 302
XmiI GTMKAC 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.