Rw1G019160

tRNA 2'-phosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0002921
Physical Location & Seq
Reverse (-)
41958397 .. 41961462
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G019160.1

Sequence Viewer

Length: 900 bp
ATGTGGGCGTTTGTCACCCATAGCCACCTTCAAATTCTACGCTACTGTCGTGCTTTCCTTCCACGTAGCCCTAAACCTCTCCCTCTTCTTTCCCTTCCCAGCCTAACGTTCATGGACAACAGAACCACCACCACCAGCAATTCCTTCGCTCCTTTTGCCCGTAATAACAGAAGTGGTGAAGGTAGAGGAGGAGGCTGGGAGGTGAAAGATGAGGGAGAGAGATCGAGAGGTCGCGGCGGAGGAGCAAGGGGCCGTGGTGGTGGCTCTGGGAAAGATAGAGTCGATGCACTCGGTAGATTATTGACACGTATATTGCGGCATATGGCTTCTGAGTTGAATTTGGATATGCGGAGTGATGGATTTGTGAGGGTTCATGATATTTTAAAGCTGAACTTGAAGACATTTGCTAATATTCCATTGAGCTCACATACTGTTGATGAGATCAAGGAGGCTGTCAGAAGGGATAATAAGCAGCGTTTCAGCCTCTTGGAAGATAATGGGGAACTTTTGATACGTGCAAACCAAGGCCACTCAACAACGATAGTTGAAACTGAAAGATTATTAAAGCCAATCCTTTCATTTGAAGAAGTTCCAGTATGCGTGCATGGAACTTATAGGAAGAATTTGGATTCAATATTAGAGTCTGGTCTGAAGTGCATGACAAGATTGCATGTACACTTTGCAAGTGGCTTGCCAACAGATGGGGAAGTGATCAGCGGTATGAGACGGAATGTTAACATTTTGATCTTCCTTGATATCAGAAAAGCTTTAGCTGAGGGAATGAAGTTCTACATTTCAGACAACAAGGTGATCTTGACTGATGGCTTTGATGGTGTTGTTCCGGTCAAGTACTTCGAGAAAATAGAATCGTGGCCTGGTAGAAAGCCCATAGCTTTTTGA

Protein Analysis

299

Amino Acids

33.57

Weight (kDa)

9.76

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTS_2-RNA PF01885 95 - 275 5.9e-57 RNA 2'-phosphotransferase, Tpt1 / KptA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 701
AccII CGCG 1 cut(s) 234
AciI CCGC 5 cut(s) 234, 237, 316, 349, 717
AclI AACGTT 1 cut(s) 107
AcsI RAATTY 3 cut(s) 33, 337, 622
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 2 cut(s) 675, 851
AfiI CCNNNNNNNGG 1 cut(s) 701
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 6 cut(s) 32, 337, 397, 548, 584, 633
AjnI CCWGG 1 cut(s) 874
AluBI AGCT 5 cut(s) 388, 423, 767, 773, 893
AluI AGCT 5 cut(s) 388, 423, 767, 773, 893
Alw21I GWGCWC 1 cut(s) 425
Alw26I GTCTC 1 cut(s) 718
AoxI GGCC 3 cut(s) 250, 526, 872
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 3 cut(s) 33, 337, 622
Asp700I GAANNNNTTC 1 cut(s) 588
AspS9I GGNCC 1 cut(s) 250
AsuHPI GGTGA 4 cut(s) 7, 188, 214, 820
BanII GRGCYC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 404
Bbv12I GWGCWC 1 cut(s) 425
BbvCI CCTCAGC 1 cut(s) 774
BbvI GCAGC 1 cut(s) 484
BccI CCATC 4 cut(s) 350, 695, 815, 824
BceAI ACGGC 1 cut(s) 237
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 876
BclI TGATCA 1 cut(s) 711
BcoDI GTCTC 1 cut(s) 718
BisI GCNGC 3 cut(s) 235, 317, 473
BlsI GCNGC 3 cut(s) 236, 318, 474
BmcAI AGTACT 1 cut(s) 851
Bme1390I CCNGG 1 cut(s) 876
BmgT120I GGNCC 1 cut(s) 250
BmiI GGNNCC 1 cut(s) 251
BmrFI CCNGG 1 cut(s) 876
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 404
Bpu10I CCTNAGC 1 cut(s) 774
BsaAI YACGTR 3 cut(s) 65, 308, 515
BsaJI CCNNGG 2 cut(s) 253, 523
BsaWI WCCGGW 1 cut(s) 841
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse1I ACTGG 1 cut(s) 593
BseBI CCWGG 1 cut(s) 876
BseDI CCNNGG 2 cut(s) 253, 523
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 2 cut(s) 321, 765
BseNI ACTGG 1 cut(s) 593
BseRI GAGGAG 3 cut(s) 201, 204, 255
BseXI GCAGC 1 cut(s) 484
BseYI CCCAGC 2 cut(s) 98, 195
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 3 cut(s) 252, 528, 874
BsiHKAI GWGCWC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 842
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 1 cut(s) 718
BsmBI CGTCTC 1 cut(s) 718
BsnI GGCC 3 cut(s) 252, 528, 874
Bsp1286I GDGCHC 1 cut(s) 425
Bsp1407I TGTACA 1 cut(s) 673
Bsp143I GATC 5 cut(s) 221, 441, 711, 744, 810
BspACI CCGC 5 cut(s) 234, 237, 316, 349, 717
BspANI GGCC 3 cut(s) 252, 528, 874
BspCNI CTCAG 2 cut(s) 322, 766
BspFNI CGCG 1 cut(s) 234
BspHI TCATGA 1 cut(s) 373
BspLI GGNNCC 1 cut(s) 251
BsrGI TGTACA 1 cut(s) 673
BsrI ACTGG 1 cut(s) 593
BssECI CCNNGG 2 cut(s) 253, 523
BssMI GATC 5 cut(s) 221, 441, 711, 744, 810
BssT1I CCWWGG 1 cut(s) 523
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 2 cut(s) 47, 433
Bst6I CTCTTC 1 cut(s) 90
BstAUI TGTACA 1 cut(s) 673
BstBAI YACGTR 3 cut(s) 65, 308, 515
BstC8I GCNNGC 2 cut(s) 602, 692
BstDEI CTNAG 2 cut(s) 330, 774
BstDSI CCRYGG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 234
BstKTI GATC 5 cut(s) 224, 444, 714, 747, 813
BstMAI GTCTC 1 cut(s) 718
BstMBI GATC 5 cut(s) 221, 441, 711, 744, 810
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 876
BstNSI RCATGY 1 cut(s) 674
BstSCI CCNGG 1 cut(s) 874
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 484
BstV2I GAAGAC 1 cut(s) 404
BsuRI GGCC 3 cut(s) 252, 528, 874
BtgI CCRYGG 1 cut(s) 253
Cac8I GCNNGC 2 cut(s) 602, 692
CciI TCATGA 1 cut(s) 373
Cfr13I GGNCC 1 cut(s) 250
Csp6I GTAC 2 cut(s) 674, 850
CviAII CATG 5 cut(s) 112, 374, 605, 658, 671
CviQI GTAC 2 cut(s) 674, 850
DdeI CTNAG 2 cut(s) 330, 774
DpnI GATC 5 cut(s) 223, 443, 713, 746, 812
DpnII GATC 5 cut(s) 221, 441, 711, 744, 810
DraI TTTAAA 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
EciI GGCGGA 1 cut(s) 252
Ecl136II GAGCTC 1 cut(s) 423
Eco130I CCWWGG 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 425
Eco32I GATATC 1 cut(s) 757
Eco53kI GAGCTC 1 cut(s) 423
Eco57I CTGAAG 1 cut(s) 671
EcoICRI GAGCTC 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 874
EcoRV GATATC 1 cut(s) 757
EcoT14I CCWWGG 1 cut(s) 523
EcoT38I GRGCYC 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 523
Esp3I CGTCTC 1 cut(s) 718
FaeI CATG 5 cut(s) 115, 377, 608, 661, 674
FalI AAGNNNNNCTT 4 cut(s) 377, 409, 797, 829
FatI CATG 5 cut(s) 111, 373, 604, 657, 670
FauNDI CATATG 1 cut(s) 321
FbaI TGATCA 1 cut(s) 711
Fnu4HI GCNGC 3 cut(s) 235, 317, 473
FriOI GRGCYC 1 cut(s) 425
Fsp4HI GCNGC 3 cut(s) 235, 317, 473
GluI GCNGC 3 cut(s) 235, 317, 473
GsaI CCCAGC 2 cut(s) 102, 199
HaeIII GGCC 3 cut(s) 252, 528, 874
HapII CCGG 1 cut(s) 842
Hin1II CATG 5 cut(s) 115, 377, 608, 661, 674
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 4 cut(s) 279, 629, 641, 866
HpaI GTTAAC 1 cut(s) 736
HpaII CCGG 1 cut(s) 842
HphI GGTGA 4 cut(s) 7, 188, 214, 820
Hpy166II GTNNAC 2 cut(s) 676, 736
Hpy188I TCNGA 5 cut(s) 331, 458, 651, 761, 799
Hpy188III TCNNGA 4 cut(s) 225, 374, 814, 856
Hpy8I GTNNAC 2 cut(s) 676, 736
HpyAV CCTTC 6 cut(s) 38, 68, 104, 154, 173, 453
HpyCH4III ACNGT 2 cut(s) 47, 433
HpyCH4IV ACGT 4 cut(s) 64, 107, 307, 514
HpyCH4V TGCA 6 cut(s) 287, 518, 604, 657, 670, 683
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 330, 774
HpySE526I ACGT 4 cut(s) 64, 107, 307, 514
Hsp92II CATG 5 cut(s) 115, 377, 608, 661, 674
Ksp22I TGATCA 1 cut(s) 711
KspAI GTTAAC 1 cut(s) 736
Kzo9I GATC 5 cut(s) 221, 441, 711, 744, 810
LmnI GCTCC 2 cut(s) 154, 242
LpnPI CCDG 9 cut(s) 112, 148, 181, 252, 606, 630, 855, 861, 888
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 1 cut(s) 274
MaeII ACGT 4 cut(s) 64, 107, 307, 514
MaeIII GTNAC 1 cut(s) 13
MalI GATC 5 cut(s) 223, 443, 713, 746, 812
MboI GATC 5 cut(s) 221, 441, 711, 744, 810
MboII GAAGA 6 cut(s) 77, 409, 503, 596, 631, 739
MhlI GDGCHC 1 cut(s) 425
MluCI AATT 4 cut(s) 33, 139, 337, 622
MlyI GAGTC 2 cut(s) 288, 650
MroXI GAANNNNTTC 1 cut(s) 588
MseI TTAA 3 cut(s) 383, 563, 735
MspA1I CMGCKG 1 cut(s) 717
MspI CCGG 1 cut(s) 842
MspR9I CCNGG 1 cut(s) 876
MvaI CCWGG 1 cut(s) 876
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 1 cut(s) 155
NdeI CATATG 1 cut(s) 321
NdeII GATC 5 cut(s) 221, 441, 711, 744, 810
NlaIII CATG 5 cut(s) 115, 377, 608, 661, 674
NlaIV GGNNCC 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 13
NspI RCATGY 1 cut(s) 674
PagI TCATGA 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PdmI GAANNNNTTC 1 cut(s) 588
PfeI GAWTC 2 cut(s) 629, 866
PflMI CCANNNNNTGG 1 cut(s) 701
PkrI GCNGC 3 cut(s) 236, 318, 474
PleI GAGTC 2 cut(s) 287, 649
PpsI GAGTC 2 cut(s) 287, 649
Ppu21I YACGTR 3 cut(s) 65, 308, 515
Psp124BI GAGCTC 1 cut(s) 425
Psp1406I AACGTT 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 2 cut(s) 98, 195
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 1 cut(s) 251
PspPI GGNCC 1 cut(s) 250
PsrI GAACNNNNNNTAC 2 cut(s) 495, 527
RsaI GTAC 2 cut(s) 675, 851
RsaNI GTAC 2 cut(s) 674, 850
SacI GAGCTC 1 cut(s) 425
SaqAI TTAA 3 cut(s) 383, 563, 735
SatI GCNGC 3 cut(s) 235, 317, 473
Sau3AI GATC 5 cut(s) 221, 441, 711, 744, 810
Sau96I GGNCC 1 cut(s) 250
ScaI AGTACT 1 cut(s) 851
SchI GAGTC 2 cut(s) 288, 650
ScrFI CCNGG 1 cut(s) 876
SduI GDGCHC 1 cut(s) 425
SfaNI GCATC 1 cut(s) 274
Sse9I AATT 4 cut(s) 33, 139, 337, 622
SsiI CCGC 5 cut(s) 234, 237, 316, 349, 717
SspI AATATT 2 cut(s) 412, 636
SstI GAGCTC 1 cut(s) 425
StyD4I CCNGG 1 cut(s) 874
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 2 cut(s) 47, 433
TaiI ACGT 4 cut(s) 67, 110, 310, 517
TaqI TCGA 3 cut(s) 224, 282, 855
TasI AATT 4 cut(s) 33, 139, 337, 622
TatI WGTACW 2 cut(s) 673, 849
TauI GCSGC 2 cut(s) 237, 319
TfiI GAWTC 2 cut(s) 629, 866
Tru1I TTAA 3 cut(s) 383, 563, 735
Tru9I TTAA 3 cut(s) 383, 563, 735
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 4 cut(s) 100, 362, 567, 797
TspGWI ACGGA 1 cut(s) 742
Van91I CCANNNNNTGG 1 cut(s) 701
XapI RAATTY 3 cut(s) 33, 337, 622
XceI RCATGY 1 cut(s) 674
XmnI GAANNNNTTC 1 cut(s) 588
ZrmI AGTACT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.