Rw7G015660

tRNA 2'-phosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002921
Physical Location & Seq
Forward (+)
15878613 .. 15883316
4704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G015660.1

Sequence Viewer

Length: 711 bp
ATGGCCTCCAAACGCATCTTGAAGGAGCTTAAGGATTTTCAGAAGGATCTTCCCACTTCCTGCAGCGCCGGACTAAGGTCTTCTACCCGAATATCAATAGCAATGGAAGCATTTGTCTTGATATCCTCAAAGAACAATGGAGTCCAGCTCTTACTATCTCTAAGAGGAGGAGGTTGGGTGGTAAAAGATGAGGGTGGGAGATCAAGAGGTAGAGACAGAGGATGGGGCTGTCGTGGCGGTGGCGACTGCTCTGGCAAAGATAGAGTTGATGCACTTGGTATATTATTGACACGTATATTGCGCCATATGGCTTCTGAGTTGAATTTTGATATGCAGAGTGATGGATTTGTGAAGGTTCATGATCATTTAGAGCTAAACTTGAAGACATTTGCTAATATTCCATTGAGCTCACATAGTGTTGATGAAAATAAGGAGGTTGTCAGAAAGGACAATAAGCAACGTTTTAGCCTCTTGGAAGAAAATGGGAAACTTTTGATACGTGCAAACCAAGGCCACTCAACAACGACGCTAGTCTTCATTCTTATAAAGACTTGTAAAGTTTTGAAGATAGGAAGAAGAACCCATTTTTCTTTGATTTCTTGGGAGAGAGTCACCAATCACCCTAAATGCTTGCGAGATATATATATATATATATATATGGATGAAACAATGGTTGTCACCAACATATATTTTAACCAAATTGAGACCTAA

Protein Analysis

236

Amino Acids

26.73

Weight (kDa)

9.51

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTS_2-RNA PF01885 93 - 176 1.6e-17 RNA 2'-phosphotransferase, Tpt1 / KptA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
AciI CCGC 1 cut(s) 237
AclI AACGTT 1 cut(s) 460
AclWI GGATC 1 cut(s) 54
AcsI RAATTY 1 cut(s) 322
AdeI CACNNNGTG 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 75
AflII CTTAAG 1 cut(s) 29
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 4 cut(s) 22, 322, 382, 565
AluBI AGCT 4 cut(s) 28, 148, 373, 408
AluI AGCT 4 cut(s) 28, 148, 373, 408
Alw21I GWGCWC 1 cut(s) 410
Alw26I GTCTC 2 cut(s) 207, 698
AlwI GGATC 1 cut(s) 54
AoxI GGCC 2 cut(s) 3, 511
ApeKI GCWGC 1 cut(s) 63
ApoI RAATTY 1 cut(s) 322
AspLEI GCGC 2 cut(s) 68, 303
AsuHPI GGTGA 3 cut(s) 604, 611, 670
BanII GRGCYC 1 cut(s) 410
BbsI GAAGAC 3 cut(s) 72, 389, 526
Bbv12I GWGCWC 1 cut(s) 410
BbvI GCAGC 1 cut(s) 75
BccI CCATC 2 cut(s) 216, 335
BclI TGATCA 1 cut(s) 361
BcoDI GTCTC 2 cut(s) 207, 698
BfaI CTAG 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 61
BfoI RGCGCY 1 cut(s) 69
BfrI CTTAAG 1 cut(s) 29
BisI GCNGC 1 cut(s) 64
BlsI GCNGC 1 cut(s) 65
BmsI GCATC 2 cut(s) 24, 259
BoxI GACNNNNGTC 2 cut(s) 76, 530
BpiI GAAGAC 3 cut(s) 72, 389, 526
BplI GAGNNNNNCTC 2 cut(s) 132, 164
BsaAI YACGTR 2 cut(s) 293, 500
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 1 cut(s) 508
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 508
BseGI GGATG 2 cut(s) 227, 667
BseLI CCNNNNNNNGG 1 cut(s) 75
BseMI GCAATG 1 cut(s) 108
BseMII CTCAG 1 cut(s) 306
BseRI GAGGAG 2 cut(s) 180, 183
BseXI GCAGC 1 cut(s) 75
BshFI GGCC 2 cut(s) 5, 513
BsiHKAI GWGCWC 1 cut(s) 410
BsiSI CCGG 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 2 cut(s) 207, 698
BsnI GGCC 2 cut(s) 5, 513
Bso31I GGTCTC 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 410
Bsp143I GATC 3 cut(s) 46, 200, 361
BspACI CCGC 1 cut(s) 237
BspANI GGCC 2 cut(s) 5, 513
BspCNI CTCAG 1 cut(s) 307
BspHI TCATGA 1 cut(s) 358
BspMAI CTGCAG 1 cut(s) 65
BspPI GGATC 1 cut(s) 54
BspTI CTTAAG 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 508
BssMI GATC 3 cut(s) 46, 200, 361
BssT1I CCWWGG 1 cut(s) 508
BstAFI CTTAAG 1 cut(s) 29
BstBAI YACGTR 2 cut(s) 293, 500
BstC8I GCNNGC 1 cut(s) 632
BstDEI CTNAG 3 cut(s) 74, 161, 315
BstF5I GGATG 2 cut(s) 227, 667
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 303
BstKTI GATC 3 cut(s) 49, 203, 364
BstMAI GTCTC 2 cut(s) 207, 698
BstMBI GATC 3 cut(s) 46, 200, 361
BstMWI GCNNNNNNNGC 2 cut(s) 107, 234
BstPAI GACNNNNGTC 2 cut(s) 76, 530
BstSFI CTRYAG 1 cut(s) 61
BstV1I GCAGC 1 cut(s) 75
BstV2I GAAGAC 3 cut(s) 72, 389, 526
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BsuRI GGCC 2 cut(s) 5, 513
BtsCI GGATG 2 cut(s) 227, 667
Cac8I GCNNGC 1 cut(s) 632
CciI TCATGA 1 cut(s) 358
CfoI GCGC 2 cut(s) 68, 303
CseI GACGC 1 cut(s) 535
CviAII CATG 1 cut(s) 359
CviJI RGCY 9 cut(s) 5, 28, 148, 228, 311, 373, 408, 468, 513
CviKI_1 RGCY 9 cut(s) 5, 28, 148, 228, 311, 373, 408, 468, 513
DdeI CTNAG 3 cut(s) 74, 161, 315
DpnI GATC 3 cut(s) 48, 202, 363
DpnII GATC 3 cut(s) 46, 200, 361
DraIII CACNNNGTG 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 408
Eco130I CCWWGG 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 410
Eco31I GGTCTC 1 cut(s) 698
Eco32I GATATC 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 408
EcoRV GATATC 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 508
EcoT38I GRGCYC 1 cut(s) 410
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 1 cut(s) 362
FatI CATG 1 cut(s) 358
FauNDI CATATG 1 cut(s) 306
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 1 cut(s) 64
FokI GGATG 2 cut(s) 234, 674
FriOI GRGCYC 1 cut(s) 410
Fsp4HI GCNGC 1 cut(s) 64
FspBI CTAG 1 cut(s) 530
GlaI GCGC 2 cut(s) 67, 302
GluI GCNGC 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 2 cut(s) 5, 513
HapII CCGG 1 cut(s) 69
HgaI GACGC 1 cut(s) 535
HhaI GCGC 2 cut(s) 68, 303
Hin1II CATG 1 cut(s) 362
Hin6I GCGC 2 cut(s) 66, 301
HinP1I GCGC 2 cut(s) 66, 301
HinfI GANTC 2 cut(s) 141, 609
HpaII CCGG 1 cut(s) 69
HphI GGTGA 3 cut(s) 604, 611, 670
Hpy188I TCNGA 3 cut(s) 42, 316, 443
Hpy188III TCNNGA 4 cut(s) 19, 118, 204, 359
Hpy99I CGWCG 1 cut(s) 529
HpyAV CCTTC 3 cut(s) 16, 37, 346
HpyCH4IV ACGT 3 cut(s) 292, 460, 499
HpyCH4V TGCA 4 cut(s) 63, 272, 334, 503
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 234
HpyF3I CTNAG 3 cut(s) 74, 161, 315
HpySE526I ACGT 3 cut(s) 292, 460, 499
Hsp92II CATG 1 cut(s) 362
HspAI GCGC 2 cut(s) 66, 301
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 3 cut(s) 46, 200, 361
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 4 cut(s) 73, 82, 158, 237
Lsp1109I GCAGC 1 cut(s) 75
LweI GCATC 2 cut(s) 24, 259
MaeI CTAG 1 cut(s) 530
MaeII ACGT 3 cut(s) 292, 460, 499
MaeIII GTNAC 2 cut(s) 610, 676
MalI GATC 3 cut(s) 48, 202, 363
MboI GATC 3 cut(s) 46, 200, 361
MboII GAAGA 8 cut(s) 41, 72, 394, 488, 526, 577, 585, 588
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 410
MluCI AATT 2 cut(s) 322, 699
MlyI GAGTC 2 cut(s) 150, 618
MseI TTAA 2 cut(s) 30, 693
MspCI CTTAAG 1 cut(s) 29
MspI CCGG 1 cut(s) 69
MwoI GCNNNNNNNGC 2 cut(s) 107, 234
NdeI CATATG 1 cut(s) 306
NdeII GATC 3 cut(s) 46, 200, 361
NlaIII CATG 1 cut(s) 362
NmuCI GTSAC 2 cut(s) 610, 676
PagI TCATGA 1 cut(s) 358
PkrI GCNGC 1 cut(s) 65
PleI GAGTC 2 cut(s) 149, 617
PpsI GAGTC 2 cut(s) 149, 617
Ppu21I YACGTR 2 cut(s) 293, 500
PshAI GACNNNNGTC 2 cut(s) 76, 530
PsiI TTATAA 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 410
Psp1406I AACGTT 1 cut(s) 460
PstI CTGCAG 1 cut(s) 65
PsuI RGATCY 1 cut(s) 46
SacI GAGCTC 1 cut(s) 410
SaqAI TTAA 2 cut(s) 30, 693
SatI GCNGC 1 cut(s) 64
Sau3AI GATC 3 cut(s) 46, 200, 361
SchI GAGTC 2 cut(s) 150, 618
SduI GDGCHC 1 cut(s) 410
SfaNI GCATC 2 cut(s) 24, 259
SfcI CTRYAG 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 2 cut(s) 322, 699
SsiI CCGC 1 cut(s) 237
SspI AATATT 1 cut(s) 397
SspMI CTAG 1 cut(s) 530
SstI GAGCTC 1 cut(s) 410
StyI CCWWGG 1 cut(s) 508
TaiI ACGT 3 cut(s) 295, 463, 502
TasI AATT 2 cut(s) 322, 699
Tru1I TTAA 2 cut(s) 30, 693
Tru9I TTAA 2 cut(s) 30, 693
TseFI GTSAC 2 cut(s) 610, 676
TseI GCWGC 1 cut(s) 63
Tsp45I GTSAC 2 cut(s) 610, 676
TspDTI ATGAA 4 cut(s) 347, 438, 526, 678
Vha464I CTTAAG 1 cut(s) 29
XapI RAATTY 1 cut(s) 322
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.