RLG00000003800

tRNA 2'-phosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0002921
Physical Location & Seq
Reverse (-)
52859390 .. 52860695
1306 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003800

Sequence Viewer

Length: 342 bp
ATGAGGGAGAGAGATCAAGAGGTCGTGGCAGAGGAAGGGGCTGTCGTAGCAGTGGCGGCGGGTAATTCACCTGACTGGCATAGGACATGTATATTGCGCCATATGGCTTCTGAGTTGAATTTGGATATGCGGAGTGATGGATTTGTGAAGGTTCATGATCTTTTAGAGCTAAACTTGAAGACATTTGCTAATATTCCCTCGAGCTCACATATTGTTGATGAGATTAAGGAGGCTGTTAGAAAGGATAATAAGCAGCGTTTCTTGGAAAAAAATGAGGAACTTTTGATACATGCAAACCAAGGCCACTCAACAACGACAACTATCCTCGTTTATCTTGCATAG

Protein Analysis

114

Amino Acids

12.78

Weight (kDa)

5.62

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTS_2-RNA PF01885 30 - 105 3.5e-18 RNA 2'-phosphotransferase, Tpt1 / KptA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 56, 59, 130
AcsI RAATTY 1 cut(s) 118
AflIII ACRYGT 1 cut(s) 86
AgsI TTSAA 2 cut(s) 118, 178
AluBI AGCT 2 cut(s) 169, 204
AluI AGCT 2 cut(s) 169, 204
Alw21I GWGCWC 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 199
AoxI GGCC 1 cut(s) 301
ApeKI GCWGC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 1 cut(s) 99
AsuHPI GGTGA 1 cut(s) 60
AvaI CYCGRG 1 cut(s) 199
BanII GRGCYC 1 cut(s) 206
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 206
BbvI GCAGC 1 cut(s) 265
BccI CCATC 1 cut(s) 131
BisI GCNGC 2 cut(s) 57, 254
BlsI GCNGC 2 cut(s) 58, 255
BmeT110I CYCGRG 1 cut(s) 199
BpiI GAAGAC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 298
Bse1I ACTGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 298
BseMII CTCAG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 80
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 199
BsnI GGCC 1 cut(s) 303
BsoBI CYCGRG 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 206
Bsp143I GATC 2 cut(s) 13, 157
BspACI CCGC 3 cut(s) 56, 59, 130
BspANI GGCC 1 cut(s) 303
BspCNI CTCAG 1 cut(s) 103
BspHI TCATGA 1 cut(s) 154
BsrI ACTGG 1 cut(s) 80
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 2 cut(s) 13, 157
BssT1I CCWWGG 1 cut(s) 298
BstDEI CTNAG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 99
BstKTI GATC 2 cut(s) 16, 160
BstMBI GATC 2 cut(s) 13, 157
BstMWI GCNNNNNNNGC 2 cut(s) 47, 56
BstNSI RCATGY 2 cut(s) 90, 293
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 1 cut(s) 185
BsuRI GGCC 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
CciI TCATGA 1 cut(s) 154
CfoI GCGC 1 cut(s) 99
CviAII CATG 3 cut(s) 87, 155, 290
CviJI RGCY 6 cut(s) 41, 107, 169, 204, 233, 303
CviKI_1 RGCY 6 cut(s) 41, 107, 169, 204, 233, 303
DdeI CTNAG 1 cut(s) 111
DpnI GATC 2 cut(s) 15, 159
DpnII GATC 2 cut(s) 13, 157
Ecl136II GAGCTC 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 204
Eco88I CYCGRG 1 cut(s) 199
EcoICRI GAGCTC 1 cut(s) 204
EcoT14I CCWWGG 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 298
FaeI CATG 3 cut(s) 90, 158, 293
FatI CATG 3 cut(s) 86, 154, 289
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 57, 254
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 2 cut(s) 57, 254
GlaI GCGC 1 cut(s) 98
GluI GCNGC 2 cut(s) 57, 254
HaeIII GGCC 1 cut(s) 303
HhaI GCGC 1 cut(s) 99
Hin1II CATG 3 cut(s) 90, 158, 293
Hin6I GCGC 1 cut(s) 97
HinP1I GCGC 1 cut(s) 97
HphI GGTGA 1 cut(s) 60
Hpy188I TCNGA 1 cut(s) 112
Hpy188III TCNNGA 2 cut(s) 17, 155
HpyAV CCTTC 2 cut(s) 29, 142
HpyCH4V TGCA 2 cut(s) 293, 338
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 56
HpyF3I CTNAG 1 cut(s) 111
Hsp92II CATG 3 cut(s) 90, 158, 293
HspAI GCGC 1 cut(s) 97
Kzo9I GATC 2 cut(s) 13, 157
LpnPI CCDG 2 cut(s) 61, 84
Lsp1109I GCAGC 1 cut(s) 265
MalI GATC 2 cut(s) 15, 159
MboI GATC 2 cut(s) 13, 157
MboII GAAGA 1 cut(s) 190
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 2 cut(s) 64, 118
MnlI CCTC 6 cut(s) 13, 25, 208, 223, 268, 335
MseI TTAA 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 47, 56
NdeI CATATG 1 cut(s) 102
NdeII GATC 2 cut(s) 13, 157
NlaIII CATG 3 cut(s) 90, 158, 293
NspI RCATGY 2 cut(s) 90, 293
PaeR7I CTCGAG 1 cut(s) 199
PagI TCATGA 1 cut(s) 154
PciI ACATGT 1 cut(s) 86
PkrI GCNGC 2 cut(s) 58, 255
PscI ACATGT 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 206
PspXI VCTCGAGB 1 cut(s) 199
PsrI GAACNNNNNNTAC 2 cut(s) 270, 302
SacI GAGCTC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 2 cut(s) 57, 254
Sau3AI GATC 2 cut(s) 13, 157
SduI GDGCHC 1 cut(s) 206
SetI ASST 5 cut(s) 24, 73, 153, 171, 206
Sfr274I CTCGAG 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 199
SmoI CTYRAG 1 cut(s) 199
Sse9I AATT 2 cut(s) 64, 118
SsiI CCGC 3 cut(s) 56, 59, 130
SspI AATATT 1 cut(s) 193
SstI GAGCTC 1 cut(s) 206
StyI CCWWGG 1 cut(s) 298
TaqI TCGA 1 cut(s) 200
TasI AATT 2 cut(s) 64, 118
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TscAI CASTG 1 cut(s) 57
TseI GCWGC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 143
TspRI CASTG 1 cut(s) 57
XapI RAATTY 1 cut(s) 118
XceI RCATGY 2 cut(s) 90, 293
XhoI CTCGAG 1 cut(s) 199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.