Rh7DG181100

tRNA 2'-phosphotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0002921
Physical Location & Seq
Forward (+)
16765058 .. 16766836
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG181100.1

Sequence Viewer

Length: 372 bp
ATGCTATCACCGCCACCTCTAGATACCTCCACGATTGAAGAGCTCCAATGTCGCCCGTCCTGTGACCACGGAAAGACGAGTCGTGAGTCGCGCACGCGATTGGGAAGGACACGTATATTGCGCCATATGGCTTCTGAGTTGAATTTGGATATGCGGAGTGATGGATTTGTGAAGGTTCATGATCTTTTAGAGCTAAACTTGAAGGCATTTGCTAATATTCCCTTAAGCTCACATATTGTTGATGAGATTAAGGAGGCTGTTAGAAAGGATAATAAGCAGCGTTTTAGCCTCTTGGAAGAAAATGAGGAGCTTTTGATGCGTGCAAACCAAGGACGCTCAACAACGGCAACTATCCTCGTTTATCTTGCATAG

Protein Analysis

123

Amino Acids

14.03

Weight (kDa)

6.91

Isoelectric Point (pI)

62.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTS_2-RNA PF01885 37 - 114 1.8e-17 RNA 2'-phosphotransferase, Tpt1 / KptA family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 91, 97
AciI CCGC 2 cut(s) 11, 154
AcsI RAATTY 1 cut(s) 142
AflII CTTAAG 1 cut(s) 223
AflIII ACRYGT 1 cut(s) 110
AgsI TTSAA 3 cut(s) 38, 142, 202
AluBI AGCT 4 cut(s) 43, 193, 228, 310
AluI AGCT 4 cut(s) 43, 193, 228, 310
Alw21I GWGCWC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 277
ApoI RAATTY 1 cut(s) 142
AspLEI GCGC 2 cut(s) 93, 123
BanII GRGCYC 1 cut(s) 45
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 289
BccI CCATC 1 cut(s) 155
BceAI ACGGC 1 cut(s) 360
BfaI CTAG 1 cut(s) 20
BfrI CTTAAG 1 cut(s) 223
BisI GCNGC 1 cut(s) 278
BlsI GCNGC 1 cut(s) 279
BmsI GCATC 1 cut(s) 306
BsaAI YACGTR 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 67, 328
BseDI CCNNGG 2 cut(s) 67, 328
BseMII CTCAG 1 cut(s) 126
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 289
Bsh1236I CGCG 2 cut(s) 91, 97
BsiHKAI GWGCWC 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 2 cut(s) 11, 154
BspCNI CTCAG 1 cut(s) 127
BspFNI CGCG 2 cut(s) 91, 97
BspHI TCATGA 1 cut(s) 178
BspQI GCTCTTC 1 cut(s) 33
BspTI CTTAAG 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 67, 328
BssMI GATC 1 cut(s) 181
BssT1I CCWWGG 1 cut(s) 328
Bst6I CTCTTC 1 cut(s) 33
BstAFI CTTAAG 1 cut(s) 223
BstBAI YACGTR 1 cut(s) 113
BstC8I GCNNGC 2 cut(s) 95, 321
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 1 cut(s) 67
BstFNI CGCG 2 cut(s) 91, 97
BstHHI GCGC 2 cut(s) 93, 123
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMWI GCNNNNNNNGC 2 cut(s) 10, 316
BstUI CGCG 2 cut(s) 91, 97
BstV1I GCAGC 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 67
Cac8I GCNNGC 2 cut(s) 95, 321
CciI TCATGA 1 cut(s) 178
CfoI GCGC 2 cut(s) 93, 123
CseI GACGC 1 cut(s) 342
CviAII CATG 1 cut(s) 179
CviJI RGCY 7 cut(s) 43, 131, 193, 228, 257, 288, 310
CviKI_1 RGCY 7 cut(s) 43, 131, 193, 228, 257, 288, 310
DdeI CTNAG 1 cut(s) 135
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 45
Eco53kI GAGCTC 1 cut(s) 43
EcoICRI GAGCTC 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 1 cut(s) 182
FaiI YATR 7 cut(s) 116, 126, 128, 152, 180, 234, 370
FatI CATG 1 cut(s) 178
FauNDI CATATG 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 1 cut(s) 278
FspBI CTAG 1 cut(s) 20
GlaI GCGC 2 cut(s) 92, 122
GluI GCNGC 1 cut(s) 278
HgaI GACGC 1 cut(s) 342
HhaI GCGC 2 cut(s) 93, 123
Hin1II CATG 1 cut(s) 182
Hin6I GCGC 2 cut(s) 91, 121
HinP1I GCGC 2 cut(s) 91, 121
HinfI GANTC 2 cut(s) 79, 86
Hpy188I TCNGA 1 cut(s) 136
Hpy188III TCNNGA 3 cut(s) 20, 83, 179
HpyAV CCTTC 3 cut(s) 99, 166, 196
HpyCH4IV ACGT 1 cut(s) 112
HpyCH4V TGCA 2 cut(s) 323, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 316
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 1 cut(s) 182
HspAI GCGC 2 cut(s) 91, 121
Kzo9I GATC 1 cut(s) 181
LguI GCTCTTC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 48, 307
LpnPI CCDG 1 cut(s) 73
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 1 cut(s) 306
MaeI CTAG 1 cut(s) 20
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 62
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 2 cut(s) 50, 308
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 1 cut(s) 142
MlyI GAGTC 2 cut(s) 88, 95
MnlI CCTC 6 cut(s) 27, 37, 247, 298, 299, 365
MseI TTAA 2 cut(s) 224, 249
MspCI CTTAAG 1 cut(s) 223
MvnI CGCG 2 cut(s) 91, 97
MwoI GCNNNNNNNGC 2 cut(s) 10, 316
NdeI CATATG 1 cut(s) 126
NdeII GATC 1 cut(s) 181
NlaIII CATG 1 cut(s) 182
NmuCI GTSAC 1 cut(s) 62
PagI TCATGA 1 cut(s) 178
PciSI GCTCTTC 1 cut(s) 33
PkrI GCNGC 1 cut(s) 279
PleI GAGTC 2 cut(s) 87, 94
PpsI GAGTC 2 cut(s) 87, 94
Ppu21I YACGTR 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 45
SacI GAGCTC 1 cut(s) 45
SapI GCTCTTC 1 cut(s) 33
SaqAI TTAA 2 cut(s) 224, 249
SatI GCNGC 1 cut(s) 278
Sau3AI GATC 1 cut(s) 181
SchI GAGTC 2 cut(s) 88, 95
SduI GDGCHC 1 cut(s) 45
SetI ASST 8 cut(s) 19, 29, 45, 115, 177, 195, 230, 312
SfaNI GCATC 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 1 cut(s) 142
SsiI CCGC 2 cut(s) 11, 154
SspI AATATT 1 cut(s) 217
SspMI CTAG 1 cut(s) 20
SstI GAGCTC 1 cut(s) 45
StyI CCWWGG 1 cut(s) 328
TaiI ACGT 1 cut(s) 115
TasI AATT 1 cut(s) 142
Tru1I TTAA 2 cut(s) 224, 249
Tru9I TTAA 2 cut(s) 224, 249
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 1 cut(s) 167
TspGWI ACGGA 1 cut(s) 84
Vha464I CTTAAG 1 cut(s) 223
XapI RAATTY 1 cut(s) 142
XbaI TCTAGA 1 cut(s) 19
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.