MD07G1249800.v1.1

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003787
Physical Location & Seq
Reverse (-)
31865550 .. 31866680
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1249800.v1.1.491

Sequence Viewer

Length: 294 bp
ATGGATGGCACCACACTTGAGAGATTGAAAGAGGTTGCTGATACAGCAAAAAATTCAGCTCCTCCGGTTCAATGCCTACCTCCTAACCACTTCATCTGTCCAATTCTTAAGGATGTGATGGATGAGCCATGTGTGGCCGCAGATGGTTACACTTATGACCGCAAAGCGATCGAGAAATGGCTCGAAGAGAACGATAACTCACCGATGACAAATTTGCCATTGCCAAACAAGGATGTTATACTAAACTATACTCTTCTTTCTGCAATTATGGAGTGGAAGACCAGAAAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr U-box Usp

Protein Analysis

98

Amino Acids

11.02

Weight (kDa)

4.86

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 28 - 96 7.1e-19 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 8
AciI CCGC 2 cut(s) 138, 160
AcoI YGGCCR 1 cut(s) 135
AcsI RAATTY 2 cut(s) 52, 211
AflII CTTAAG 1 cut(s) 107
AgsI TTSAA 2 cut(s) 28, 71
AluBI AGCT 1 cut(s) 59
AluI AGCT 1 cut(s) 59
AoxI GGCC 1 cut(s) 135
ApoI RAATTY 2 cut(s) 52, 211
AsuHPI GGTGA 1 cut(s) 192
BanI GGYRCC 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 284
BccI CCATC 2 cut(s) 112, 137
BcgI CGANNNNNNTGC 2 cut(s) 151, 185
BfrI CTTAAG 1 cut(s) 107
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 10
BpiI GAAGAC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 38
BsaWI WCCGGW 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 218
BseGI GGATG 4 cut(s) 10, 118, 127, 238
BseMI GCAATG 1 cut(s) 218
BseRI GAGGAG 1 cut(s) 51
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 8
BsiEI CGRYCG 1 cut(s) 171
BsiSI CCGG 1 cut(s) 65
BsnI GGCC 1 cut(s) 137
Bsp143I GATC 1 cut(s) 168
BspACI CCGC 2 cut(s) 138, 160
BspANI GGCC 1 cut(s) 137
BspLI GGNNCC 1 cut(s) 10
BspT107I GGYRCC 1 cut(s) 8
BspTI CTTAAG 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 218
BssMI GATC 1 cut(s) 168
Bst6I CTCTTC 2 cut(s) 180, 258
BstAFI CTTAAG 1 cut(s) 107
BstF5I GGATG 4 cut(s) 10, 118, 127, 238
BstKTI GATC 1 cut(s) 171
BstMBI GATC 1 cut(s) 168
BstMCI CGRYCG 1 cut(s) 171
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 284
BsuRI GGCC 1 cut(s) 137
BtsCI GGATG 4 cut(s) 10, 118, 127, 238
CviAII CATG 1 cut(s) 129
CviJI RGCY 4 cut(s) 59, 127, 137, 181
CviKI_1 RGCY 4 cut(s) 59, 127, 137, 181
DpnI GATC 1 cut(s) 170
DpnII GATC 1 cut(s) 168
EaeI YGGCCR 1 cut(s) 135
Eam1104I CTCTTC 2 cut(s) 180, 258
EarI CTCTTC 2 cut(s) 180, 258
FaeI CATG 1 cut(s) 132
FaiI YATR 5 cut(s) 130, 156, 239, 249, 269
FatI CATG 1 cut(s) 128
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 4 cut(s) 17, 125, 134, 245
Fsp4HI GCNGC 1 cut(s) 138
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 65
Hin1II CATG 1 cut(s) 132
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 192
Hpy188III TCNNGA 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
Hsp92II CATG 1 cut(s) 132
Kzo9I GATC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 146
MalI GATC 1 cut(s) 170
MboI GATC 1 cut(s) 168
MboII GAAGA 3 cut(s) 197, 245, 289
MluCI AATT 4 cut(s) 52, 102, 211, 264
MnlI CCTC 3 cut(s) 25, 72, 90
MseI TTAA 1 cut(s) 108
MspCI CTTAAG 1 cut(s) 107
MspI CCGG 1 cut(s) 65
MwoI GCNNNNNNNGC 1 cut(s) 44
NdeII GATC 1 cut(s) 168
NlaIII CATG 1 cut(s) 132
NlaIV GGNNCC 1 cut(s) 10
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PkrI GCNGC 1 cut(s) 139
Ple19I CGATCG 1 cut(s) 171
PspN4I GGNNCC 1 cut(s) 10
PvuI CGATCG 1 cut(s) 171
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 1 cut(s) 168
SetI ASST 3 cut(s) 36, 61, 82
SgeI CNNG 6 cut(s) 29, 77, 141, 184, 194, 241
SmlI CTYRAG 2 cut(s) 17, 107
SmoI CTYRAG 2 cut(s) 17, 107
Sse9I AATT 4 cut(s) 52, 102, 211, 264
SsiI CCGC 2 cut(s) 138, 160
TaqI TCGA 2 cut(s) 171, 183
TasI AATT 4 cut(s) 52, 102, 211, 264
TauI GCSGC 1 cut(s) 140
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TspDTI ATGAA 1 cut(s) 82
Vha464I CTTAAG 1 cut(s) 107
XapI RAATTY 2 cut(s) 52, 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.