Rh2DG231300

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003787
Physical Location & Seq
Forward (+)
22972141 .. 22973064
924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG231300.1

Sequence Viewer

Length: 504 bp
ATGTTGGGCTTCTGCTCCGAGCTCAAGTGCTTTGTCTATGAACATATAGAGTATAGTCCCATGCTGCACGAGGTCGTCAAGCAAAGCCTTCGTTGGCACGATCGCATTCGTATTGCCAGTCAGATTTGCTCGGCCTTGTCCTACATTATTAAGAGGGCTAGGTGTGAACCTATTGTTCCTGACTATCTCAATGCATCCAACATTATTATCGATCGCTACCTTAATGCCAAAATCAATGTTGTGCTGAAAAGATGCGACAAGCCGGATTTTGCATGGGATGTAAAGACTTTTGGCTATCTGGAAGTAATGAAGGATCCACATGTGGCTGCTGATGGATTTTCCTATGAGCGAGAAGGCATAGAGAAATGGTTCCAAACCGGGCACGACACATCACCAATGACCAATTTGAAGCTCAAGAATAAAATTCTTACCCGTAATGGTACCCTTAAGCTTCTTATCAAAGACTGGTATCGTGAAAGATCCATTCCTTATCCAAATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr U-box

Protein Analysis

167

Amino Acids

19.64

Weight (kDa)

8.92

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 100 - 161 3.8e-12 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 440
AclWI GGATC 3 cut(s) 308, 321, 474
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 1 cut(s) 442
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 1 cut(s) 319
AgsI TTSAA 1 cut(s) 409
AluBI AGCT 3 cut(s) 22, 412, 451
AluI AGCT 3 cut(s) 22, 412, 451
Alw21I GWGCWC 1 cut(s) 24
AlwI GGATC 3 cut(s) 308, 321, 474
AoxI GGCC 1 cut(s) 132
ApeKI GCWGC 2 cut(s) 64, 326
ApoI RAATTY 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 368
Asp718I GGTACC 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 379
AsuHPI GGTGA 1 cut(s) 384
BaeGI GKGCMC 1 cut(s) 384
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 24
BauI CACGAG 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 2 cut(s) 51, 313
BccI CCATC 1 cut(s) 326
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BcnI CCSGG 1 cut(s) 379
BfaI CTAG 1 cut(s) 159
BfrI CTTAAG 1 cut(s) 446
BisI GCNGC 2 cut(s) 65, 327
BlsI GCNGC 2 cut(s) 66, 328
Bme1390I CCNGG 1 cut(s) 379
BmiI GGNNCC 3 cut(s) 315, 371, 442
BmrFI CCNGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 203, 242
BpuEI CTTGAG 2 cut(s) 8, 398
BpuMI CCSGG 1 cut(s) 379
Bsa29I ATCGAT 1 cut(s) 210
Bse1I ACTGG 2 cut(s) 117, 470
BseCI ATCGAT 1 cut(s) 210
BseGI GGATG 2 cut(s) 194, 283
BseNI ACTGG 2 cut(s) 117, 470
BseSI GKGCMC 1 cut(s) 384
BseXI GCAGC 2 cut(s) 51, 313
BsgI GTGCAG 1 cut(s) 50
Bsh1285I CGRYCG 2 cut(s) 103, 214
BshFI GGCC 1 cut(s) 134
BshNI GGYRCC 1 cut(s) 440
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 2 cut(s) 103, 214
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 2 cut(s) 263, 378
BslFI GGGAC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 42
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 24, 384
Bsp143I GATC 4 cut(s) 100, 211, 313, 479
BspANI GGCC 1 cut(s) 134
BspDI ATCGAT 1 cut(s) 210
BspLI GGNNCC 3 cut(s) 315, 371, 442
BspPI GGATC 3 cut(s) 308, 321, 474
BspT107I GGYRCC 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 446
BsrI ACTGG 2 cut(s) 117, 470
BssMI GATC 4 cut(s) 100, 211, 313, 479
BssSI CACGAG 1 cut(s) 68
Bst2BI CACGAG 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 446
BstF5I GGATG 2 cut(s) 194, 283
BstKTI GATC 4 cut(s) 103, 214, 316, 482
BstMBI GATC 4 cut(s) 100, 211, 313, 479
BstMCI CGRYCG 2 cut(s) 103, 214
BstNSI RCATGY 1 cut(s) 323
BstSCI CCNGG 1 cut(s) 377
BstSLI GKGCMC 1 cut(s) 384
BstV1I GCAGC 2 cut(s) 51, 313
BstX2I RGATCY 2 cut(s) 313, 479
BstYI RGATCY 2 cut(s) 313, 479
Bsu15I ATCGAT 1 cut(s) 210
BsuRI GGCC 1 cut(s) 134
BsuTUI ATCGAT 1 cut(s) 210
BtsCI GGATG 2 cut(s) 194, 283
ClaI ATCGAT 1 cut(s) 210
Csp6I GTAC 1 cut(s) 441
CviAII CATG 3 cut(s) 61, 273, 320
CviQI GTAC 1 cut(s) 441
DpnI GATC 4 cut(s) 102, 213, 315, 481
DpnII GATC 4 cut(s) 100, 211, 313, 479
Ecl136II GAGCTC 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 24
Eco53kI GAGCTC 1 cut(s) 22
EcoICRI GAGCTC 1 cut(s) 22
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 24
FaeI CATG 3 cut(s) 64, 276, 323
FaqI GGGAC 1 cut(s) 42
FatI CATG 3 cut(s) 60, 272, 319
Fnu4HI GCNGC 2 cut(s) 65, 327
FokI GGATG 2 cut(s) 181, 290
FriOI GRGCYC 1 cut(s) 24
Fsp4HI GCNGC 2 cut(s) 65, 327
FspBI CTAG 1 cut(s) 159
GluI GCNGC 2 cut(s) 65, 327
HaeIII GGCC 1 cut(s) 134
HapII CCGG 2 cut(s) 263, 378
Hin1II CATG 3 cut(s) 64, 276, 323
HindIII AAGCTT 1 cut(s) 449
HpaII CCGG 2 cut(s) 263, 378
HphI GGTGA 1 cut(s) 384
Hpy166II GTNNAC 1 cut(s) 167
Hpy188I TCNGA 2 cut(s) 19, 123
Hpy188III TCNNGA 4 cut(s) 179, 299, 415, 473
Hpy8I GTNNAC 1 cut(s) 167
HpyAV CCTTC 3 cut(s) 98, 304, 347
HpyCH4V TGCA 3 cut(s) 67, 194, 272
Hsp92II CATG 3 cut(s) 64, 276, 323
KpnI GGTACC 1 cut(s) 444
Kzo9I GATC 4 cut(s) 100, 211, 313, 479
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 6 cut(s) 130, 192, 276, 284, 391, 451
Lsp1109I GCAGC 2 cut(s) 51, 313
LweI GCATC 2 cut(s) 203, 242
MaeI CTAG 1 cut(s) 159
MalI GATC 4 cut(s) 102, 213, 315, 481
MboI GATC 4 cut(s) 100, 211, 313, 479
MflI RGATCY 2 cut(s) 313, 479
MhlI GDGCHC 2 cut(s) 24, 384
MluCI AATT 2 cut(s) 403, 423
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 2 cut(s) 64, 147
Mph1103I ATGCAT 1 cut(s) 196
MroXI GAANNNNTTC 1 cut(s) 368
MseI TTAA 3 cut(s) 150, 222, 447
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 2 cut(s) 263, 378
MspR9I CCNGG 1 cut(s) 379
Mva1269I GAATGC 1 cut(s) 105
NciI CCSGG 1 cut(s) 379
NdeII GATC 4 cut(s) 100, 211, 313, 479
NlaIII CATG 3 cut(s) 64, 276, 323
NlaIV GGNNCC 3 cut(s) 315, 371, 442
NmeAIII GCCGAG 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 196
NspI RCATGY 1 cut(s) 323
PciI ACATGT 1 cut(s) 319
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 368
PkrI GCNGC 2 cut(s) 66, 328
Ple19I CGATCG 2 cut(s) 103, 214
PscI ACATGT 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 24
PspN4I GGNNCC 3 cut(s) 315, 371, 442
PsuI RGATCY 2 cut(s) 313, 479
PvuI CGATCG 2 cut(s) 103, 214
RsaI GTAC 1 cut(s) 442
RsaNI GTAC 1 cut(s) 441
SacI GAGCTC 1 cut(s) 24
SaqAI TTAA 3 cut(s) 150, 222, 447
SatI GCNGC 2 cut(s) 65, 327
Sau3AI GATC 4 cut(s) 100, 211, 313, 479
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 2 cut(s) 24, 384
SetI ASST 7 cut(s) 24, 75, 164, 172, 222, 414, 453
SfaNI GCATC 2 cut(s) 203, 242
SmlI CTYRAG 3 cut(s) 23, 413, 446
SmoI CTYRAG 3 cut(s) 23, 413, 446
Sse9I AATT 2 cut(s) 403, 423
SspMI CTAG 1 cut(s) 159
SstI GAGCTC 1 cut(s) 24
StyD4I CCNGG 1 cut(s) 377
TaqI TCGA 1 cut(s) 210
TasI AATT 2 cut(s) 403, 423
Tru1I TTAA 3 cut(s) 150, 222, 447
Tru9I TTAA 3 cut(s) 150, 222, 447
TseI GCWGC 2 cut(s) 64, 326
TspDTI ATGAA 2 cut(s) 54, 323
Vha464I CTTAAG 1 cut(s) 446
XapI RAATTY 1 cut(s) 423
XceI RCATGY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 368
XspI CTAG 1 cut(s) 159
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.