Rh2AG223700

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003787
Physical Location & Seq
Forward (+)
23663037 .. 23664499
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG223700.1

Sequence Viewer

Length: 606 bp
ATGAAGCAGTTTCCATCAAATTCCTCACCTCCCCCCAAGAACATGTTTTTGAATCTAGGGGCTAAGTCCCTCAGACTAATTAGACACCCTCACCTTCTTTCAATGTTGGGCTTCTGCTCCGAGCTCAAGTGCTTTGTCTATGAACATATAGAGTATAGTCCCATGCTGCACGAGGTCGTCAAGCAAAGCCTTCGTTGGCACGATCGCATTCGTATTGCCAGTCAGATTTGCTCGGCCTTGTCCTACATTATTAAGAGGGCTAGGTGTGAACCTATTGTTCCTGACTATCTCAATGCATCCAACATTATTATCGATCGCTACCTTAATGCCAAAATCAATGTTGTGCTGAAAAGATGCGACAAGCCGGATTTTGCATGGGATGTAAAGACTTTTGGCTATCTGGAAGTAATGAAGGATCCACATGTGGCTGCTGATGGATTTTCCTATGAGCGAGAAGGCATAGAGAAATGGTTCCAAACCGGGCACGACACATCACCAATGACCAATTTGAAGCTCAAGAATAAAATTCTTACCCGTAATGGTACCCTTAAGCTTCTTATCAAAGACTGGTATCGTGAAAGATCCATTCCTTATCCAAATCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr U-box

Protein Analysis

201

Amino Acids

23.48

Weight (kDa)

9.41

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 5 - 112 1.9e-07 Protein tyrosine and serine/threonine kinase
U-box PF04564 134 - 195 5.6e-12 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 542
AclWI GGATC 3 cut(s) 410, 423, 576
AcsI RAATTY 2 cut(s) 19, 525
AfaI GTAC 1 cut(s) 544
AflII CTTAAG 1 cut(s) 548
AflIII ACRYGT 2 cut(s) 42, 421
AgsI TTSAA 3 cut(s) 52, 102, 511
AluBI AGCT 3 cut(s) 124, 514, 553
AluI AGCT 3 cut(s) 124, 514, 553
Alw21I GWGCWC 1 cut(s) 126
AlwI GGATC 3 cut(s) 410, 423, 576
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 166, 428
ApoI RAATTY 2 cut(s) 19, 525
Asp700I GAANNNNTTC 1 cut(s) 470
Asp718I GGTACC 1 cut(s) 542
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 18, 83, 486
BaeGI GKGCMC 1 cut(s) 486
BamHI GGATCC 1 cut(s) 415
BanI GGYRCC 1 cut(s) 542
BanII GRGCYC 1 cut(s) 126
BauI CACGAG 1 cut(s) 170
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 153, 415
BccI CCATC 2 cut(s) 22, 428
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BcnI CCSGG 1 cut(s) 481
BfaI CTAG 2 cut(s) 56, 261
BfrI CTTAAG 1 cut(s) 548
BisI GCNGC 2 cut(s) 167, 429
BlsI GCNGC 2 cut(s) 168, 430
Bme1390I CCNGG 1 cut(s) 481
BmiI GGNNCC 3 cut(s) 417, 473, 544
BmrFI CCNGG 1 cut(s) 481
BmsI GCATC 2 cut(s) 305, 344
BpuEI CTTGAG 2 cut(s) 110, 500
BpuMI CCSGG 1 cut(s) 481
Bsa29I ATCGAT 1 cut(s) 312
Bse1I ACTGG 2 cut(s) 219, 572
BseCI ATCGAT 1 cut(s) 312
BseGI GGATG 2 cut(s) 296, 385
BseMII CTCAG 1 cut(s) 85
BseNI ACTGG 2 cut(s) 219, 572
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 2 cut(s) 153, 415
BsgI GTGCAG 1 cut(s) 152
Bsh1285I CGRYCG 2 cut(s) 205, 316
BshFI GGCC 1 cut(s) 236
BshNI GGYRCC 1 cut(s) 542
BshVI ATCGAT 1 cut(s) 312
BsiEI CGRYCG 2 cut(s) 205, 316
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 2 cut(s) 365, 480
BslFI GGGAC 2 cut(s) 52, 144
BsmFI GGGAC 2 cut(s) 52, 144
BsmI GAATGC 1 cut(s) 207
BsnI GGCC 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 126, 486
Bsp143I GATC 4 cut(s) 202, 313, 415, 581
BspANI GGCC 1 cut(s) 236
BspCNI CTCAG 1 cut(s) 84
BspDI ATCGAT 1 cut(s) 312
BspLI GGNNCC 3 cut(s) 417, 473, 544
BspPI GGATC 3 cut(s) 410, 423, 576
BspT107I GGYRCC 1 cut(s) 542
BspTI CTTAAG 1 cut(s) 548
BsrI ACTGG 2 cut(s) 219, 572
BssMI GATC 4 cut(s) 202, 313, 415, 581
BssSI CACGAG 1 cut(s) 170
Bst2BI CACGAG 1 cut(s) 170
BstAFI CTTAAG 1 cut(s) 548
BstDEI CTNAG 2 cut(s) 63, 71
BstF5I GGATG 2 cut(s) 296, 385
BstKTI GATC 4 cut(s) 205, 316, 418, 584
BstMBI GATC 4 cut(s) 202, 313, 415, 581
BstMCI CGRYCG 2 cut(s) 205, 316
BstNSI RCATGY 2 cut(s) 46, 425
BstSCI CCNGG 1 cut(s) 479
BstSLI GKGCMC 1 cut(s) 486
BstV1I GCAGC 2 cut(s) 153, 415
BstX2I RGATCY 2 cut(s) 415, 581
BstYI RGATCY 2 cut(s) 415, 581
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 1 cut(s) 236
BsuTUI ATCGAT 1 cut(s) 312
BtsCI GGATG 2 cut(s) 296, 385
ClaI ATCGAT 1 cut(s) 312
Csp6I GTAC 1 cut(s) 543
CviAII CATG 4 cut(s) 43, 163, 375, 422
CviQI GTAC 1 cut(s) 543
DdeI CTNAG 2 cut(s) 63, 71
DpnI GATC 4 cut(s) 204, 315, 417, 583
DpnII GATC 4 cut(s) 202, 313, 415, 581
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 126
FaeI CATG 4 cut(s) 46, 166, 378, 425
FaqI GGGAC 2 cut(s) 52, 144
FatI CATG 4 cut(s) 42, 162, 374, 421
Fnu4HI GCNGC 2 cut(s) 167, 429
FokI GGATG 2 cut(s) 283, 392
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 2 cut(s) 167, 429
FspBI CTAG 2 cut(s) 56, 261
GluI GCNGC 2 cut(s) 167, 429
HaeIII GGCC 1 cut(s) 236
HapII CCGG 2 cut(s) 365, 480
Hin1II CATG 4 cut(s) 46, 166, 378, 425
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 1 cut(s) 52
HpaII CCGG 2 cut(s) 365, 480
HphI GGTGA 3 cut(s) 18, 83, 486
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 3 cut(s) 74, 121, 225
Hpy188III TCNNGA 4 cut(s) 281, 401, 517, 575
Hpy8I GTNNAC 1 cut(s) 269
HpyAV CCTTC 4 cut(s) 104, 200, 406, 449
HpyCH4V TGCA 3 cut(s) 169, 296, 374
HpyF3I CTNAG 2 cut(s) 63, 71
Hsp92II CATG 4 cut(s) 46, 166, 378, 425
KpnI GGTACC 1 cut(s) 546
Kzo9I GATC 4 cut(s) 202, 313, 415, 581
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 6 cut(s) 232, 294, 378, 386, 493, 553
Lsp1109I GCAGC 2 cut(s) 153, 415
LweI GCATC 2 cut(s) 305, 344
MaeI CTAG 2 cut(s) 56, 261
MalI GATC 4 cut(s) 204, 315, 417, 583
MboI GATC 4 cut(s) 202, 313, 415, 581
MflI RGATCY 2 cut(s) 415, 581
MhlI GDGCHC 2 cut(s) 126, 486
MluCI AATT 4 cut(s) 19, 78, 505, 525
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 6 cut(s) 34, 39, 80, 99, 166, 249
Mph1103I ATGCAT 1 cut(s) 298
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 3 cut(s) 252, 324, 549
MspCI CTTAAG 1 cut(s) 548
MspI CCGG 2 cut(s) 365, 480
MspR9I CCNGG 1 cut(s) 481
Mva1269I GAATGC 1 cut(s) 207
NciI CCSGG 1 cut(s) 481
NdeII GATC 4 cut(s) 202, 313, 415, 581
NlaIII CATG 4 cut(s) 46, 166, 378, 425
NlaIV GGNNCC 3 cut(s) 417, 473, 544
NmeAIII GCCGAG 1 cut(s) 212
NsiI ATGCAT 1 cut(s) 298
NspI RCATGY 2 cut(s) 46, 425
PciI ACATGT 2 cut(s) 42, 421
PctI GAATGC 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 470
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 2 cut(s) 168, 430
Ple19I CGATCG 2 cut(s) 205, 316
PscI ACATGT 2 cut(s) 42, 421
Psp124BI GAGCTC 1 cut(s) 126
PspN4I GGNNCC 3 cut(s) 417, 473, 544
PsuI RGATCY 2 cut(s) 415, 581
PvuI CGATCG 2 cut(s) 205, 316
RsaI GTAC 1 cut(s) 544
RsaNI GTAC 1 cut(s) 543
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 252, 324, 549
SatI GCNGC 2 cut(s) 167, 429
Sau3AI GATC 4 cut(s) 202, 313, 415, 581
ScrFI CCNGG 1 cut(s) 481
SduI GDGCHC 2 cut(s) 126, 486
SetI ASST 9 cut(s) 31, 96, 126, 177, 266, 274, 324, 516, 555
SfaNI GCATC 2 cut(s) 305, 344
SmlI CTYRAG 3 cut(s) 125, 515, 548
SmoI CTYRAG 3 cut(s) 125, 515, 548
Sse9I AATT 4 cut(s) 19, 78, 505, 525
SspMI CTAG 2 cut(s) 56, 261
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 1 cut(s) 479
TaqI TCGA 1 cut(s) 312
TasI AATT 4 cut(s) 19, 78, 505, 525
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 3 cut(s) 252, 324, 549
Tru9I TTAA 3 cut(s) 252, 324, 549
TseI GCWGC 2 cut(s) 166, 428
TspDTI ATGAA 3 cut(s) 17, 156, 425
Vha464I CTTAAG 1 cut(s) 548
XapI RAATTY 2 cut(s) 19, 525
XceI RCATGY 2 cut(s) 46, 425
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 2 cut(s) 56, 261
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.