MD08G1015100.v1.1

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003608
Physical Location & Seq
Reverse (-)
1172890 .. 1173342
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1015100.v1.1.491

Sequence Viewer

Length: 453 bp
ATGTCAGCCGGACTCGGAAAATGCAGCAAGATCCGCCACATTGTGCGGCTCCACCAGTTGCTGCGGCGGTGGCGCAGCAAGGCCTGCACGTCCAACAAGCGCATACCCTCCGATGTTCCCACTGGACACGTCGCAGTCTGCGTGGGGACCAGCCGCACCAGATTCGTGGTGCGCGCGTCGTACTTGAACCACCCCGTCTTCAAAAAGCTCCTTGTGCAAGCCGAGGAGGAGTACGGCTTCTCGAACAGCGGCCCGCTCGCGATCCCCTGCGACGAGTCGCTGTTCGAGGAGGTTCTCCGATTCATTTCCCGCTCCGAGTCGGGTAAGTCGACCCGGTTAGTCAACGTCGACGACTTCCAGAAATACTGCCACGTTGGCGCCCGGAGTAACCCCGAATTGTGGGCCGATTCTCGACCGCTGCTTCGTGGATTTTCCGAGAAGACAATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

151

Amino Acids

17.02

Weight (kDa)

9.79

Isoelectric Point (pI)

61.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 16 - 102 9.8e-30 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AccBSI CCGCTC 2 cut(s) 256, 312
AccI GTMKAC 2 cut(s) 329, 348
AccII CGCG 3 cut(s) 174, 176, 260
AciI CCGC 9 cut(s) 34, 46, 64, 67, 154, 249, 254, 310, 416
AclWI GGATC 2 cut(s) 25, 256
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 43
AfaI GTAC 2 cut(s) 182, 233
AfiI CCNNNNNNNGG 1 cut(s) 399
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 2 cut(s) 187, 202
AjiI CACGTC 2 cut(s) 90, 130
AluBI AGCT 1 cut(s) 208
AluI AGCT 1 cut(s) 208
AlwI GGATC 2 cut(s) 25, 256
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 3 cut(s) 81, 250, 402
ApeKI GCWGC 4 cut(s) 24, 61, 75, 418
ArsI GACNNNNNNTTYG 2 cut(s) 405, 437
AspLEI GCGC 5 cut(s) 75, 102, 174, 176, 380
AspS9I GGNCC 3 cut(s) 147, 251, 402
AsuC2I CCSGG 2 cut(s) 334, 382
AvaII GGWCC 1 cut(s) 147
BanI GGYRCC 1 cut(s) 377
BbsI GAAGAC 2 cut(s) 190, 446
BbvI GCAGC 4 cut(s) 36, 48, 87, 405
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BcnI CCSGG 2 cut(s) 334, 382
BfoI RGCGCY 1 cut(s) 381
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 8 cut(s) 25, 47, 62, 65, 76, 154, 250, 419
BlsI GCNGC 8 cut(s) 26, 48, 63, 66, 77, 155, 251, 420
Bme1390I CCNGG 2 cut(s) 334, 382
Bme18I GGWCC 1 cut(s) 147
BmgBI CACGTC 2 cut(s) 90, 130
BmgT120I GGNCC 3 cut(s) 147, 251, 402
BmiI GGNNCC 3 cut(s) 50, 148, 379
BmrFI CCNGG 2 cut(s) 334, 382
BpiI GAAGAC 2 cut(s) 190, 446
BpuMI CCSGG 2 cut(s) 334, 382
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 2 cut(s) 55, 127
BseDI CCNNGG 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 399
BseNI ACTGG 2 cut(s) 55, 127
BsePI GCGCGC 1 cut(s) 172
BseRI GAGGAG 3 cut(s) 239, 242, 302
BseXI GCAGC 4 cut(s) 36, 48, 87, 405
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 3 cut(s) 174, 176, 260
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 83, 252, 404
BshNI GGYRCC 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 416
BsiSI CCGG 3 cut(s) 9, 334, 382
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 399
BsmFI GGGAC 1 cut(s) 160
BsnI GGCC 3 cut(s) 83, 252, 404
Bsp143I GATC 2 cut(s) 30, 261
Bsp68I TCGCGA 1 cut(s) 260
BspACI CCGC 9 cut(s) 34, 46, 64, 67, 154, 249, 254, 310, 416
BspANI GGCC 3 cut(s) 83, 252, 404
BspFNI CGCG 3 cut(s) 174, 176, 260
BspLI GGNNCC 3 cut(s) 50, 148, 379
BspPI GGATC 2 cut(s) 25, 256
BspT107I GGYRCC 1 cut(s) 377
BsrBI CCGCTC 2 cut(s) 256, 312
BsrI ACTGG 2 cut(s) 55, 127
BssECI CCNNGG 1 cut(s) 222
BssHII GCGCGC 1 cut(s) 172
BssMI GATC 2 cut(s) 30, 261
BssNI GRCGYC 1 cut(s) 378
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 1 cut(s) 84
BstC8I GCNNGC 5 cut(s) 85, 174, 219, 254, 258
BstFNI CGCG 3 cut(s) 174, 176, 260
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 5 cut(s) 75, 102, 174, 176, 380
BstKTI GATC 2 cut(s) 33, 264
BstMBI GATC 2 cut(s) 30, 261
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 5 cut(s) 33, 70, 84, 214, 375
BstSCI CCNGG 2 cut(s) 332, 380
BstUI CGCG 3 cut(s) 174, 176, 260
BstV1I GCAGC 4 cut(s) 36, 48, 87, 405
BstV2I GAAGAC 2 cut(s) 190, 446
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 166
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 3 cut(s) 83, 252, 404
BtrI CACGTC 2 cut(s) 90, 130
BtsIMutI CAGTG 1 cut(s) 120
BtuMI TCGCGA 1 cut(s) 260
Cac8I GCNNGC 5 cut(s) 85, 174, 219, 254, 258
CaiI CAGNNNCTG 1 cut(s) 61
CfoI GCGC 5 cut(s) 75, 102, 174, 176, 380
Cfr13I GGNCC 3 cut(s) 147, 251, 402
CseI GACGC 1 cut(s) 165
Csp6I GTAC 2 cut(s) 181, 232
CviJI RGCY 9 cut(s) 8, 49, 83, 153, 208, 221, 237, 252, 404
CviKI_1 RGCY 9 cut(s) 8, 49, 83, 153, 208, 221, 237, 252, 404
CviQI GTAC 2 cut(s) 181, 232
DinI GGCGCC 1 cut(s) 379
DpnI GATC 2 cut(s) 32, 263
DpnII GATC 2 cut(s) 30, 261
DraIII CACNNNGTG 1 cut(s) 43
EciI GGCGGA 1 cut(s) 23
Eco147I AGGCCT 1 cut(s) 83
Eco47I GGWCC 1 cut(s) 147
EgeI GGCGCC 1 cut(s) 379
EheI GGCGCC 1 cut(s) 379
FaiI YATR 1 cut(s) 104
FaqI GGGAC 1 cut(s) 160
FauI CCCGC 2 cut(s) 261, 317
FblI GTMKAC 2 cut(s) 329, 348
Fnu4HI GCNGC 8 cut(s) 25, 47, 62, 65, 76, 154, 250, 419
Fsp4HI GCNGC 8 cut(s) 25, 47, 62, 65, 76, 154, 250, 419
GlaI GCGC 5 cut(s) 74, 101, 173, 175, 379
GluI GCNGC 8 cut(s) 25, 47, 62, 65, 76, 154, 250, 419
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 3 cut(s) 83, 252, 404
HapII CCGG 3 cut(s) 9, 334, 382
HgaI GACGC 1 cut(s) 165
HhaI GCGC 5 cut(s) 75, 102, 174, 176, 380
Hin1I GRCGYC 1 cut(s) 378
Hin6I GCGC 5 cut(s) 73, 100, 172, 174, 378
HinP1I GCGC 5 cut(s) 73, 100, 172, 174, 378
HincII GTYRAC 3 cut(s) 330, 343, 349
HindII GTYRAC 3 cut(s) 330, 343, 349
HinfI GANTC 6 cut(s) 12, 162, 275, 300, 317, 407
HpaII CCGG 3 cut(s) 9, 334, 382
Hpy166II GTNNAC 3 cut(s) 330, 343, 349
Hpy188I TCNGA 5 cut(s) 17, 112, 299, 316, 436
Hpy188III TCNNGA 4 cut(s) 241, 259, 358, 411
Hpy8I GTNNAC 3 cut(s) 330, 343, 349
Hpy99I CGWCG 5 cut(s) 134, 181, 275, 350, 353
HpyCH4IV ACGT 4 cut(s) 89, 129, 345, 372
HpyCH4V TGCA 3 cut(s) 24, 87, 217
HpyF10VI GCNNNNNNNGC 5 cut(s) 33, 70, 84, 214, 375
HpySE526I ACGT 4 cut(s) 89, 129, 345, 372
Hsp92I GRCGYC 1 cut(s) 378
HspAI GCGC 5 cut(s) 73, 100, 172, 174, 378
KasI GGCGCC 1 cut(s) 377
Kzo9I GATC 2 cut(s) 30, 261
LmnI GCTCC 3 cut(s) 54, 213, 317
Lsp1109I GCAGC 4 cut(s) 36, 48, 87, 405
MaeII ACGT 4 cut(s) 89, 129, 345, 372
MaeIII GTNAC 1 cut(s) 386
MalI GATC 2 cut(s) 32, 263
MbiI CCGCTC 2 cut(s) 256, 312
MboI GATC 2 cut(s) 30, 261
MboII GAAGA 2 cut(s) 190, 451
MflI RGATCY 1 cut(s) 30
MluCI AATT 2 cut(s) 395, 444
Mly113I GGCGCC 1 cut(s) 378
MlyI GAGTC 3 cut(s) 6, 284, 326
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 5 cut(s) 118, 217, 220, 280, 283
MspA1I CMGCKG 2 cut(s) 249, 418
MspI CCGG 3 cut(s) 9, 334, 382
MspR9I CCNGG 2 cut(s) 334, 382
MvnI CGCG 3 cut(s) 174, 176, 260
MwoI GCNNNNNNNGC 5 cut(s) 33, 70, 84, 214, 375
NarI GGCGCC 1 cut(s) 378
NciI CCSGG 2 cut(s) 334, 382
NdeII GATC 2 cut(s) 30, 261
NlaIV GGNNCC 3 cut(s) 50, 148, 379
NmeAIII GCCGAG 1 cut(s) 247
NruI TCGCGA 1 cut(s) 260
PauI GCGCGC 1 cut(s) 172
PceI AGGCCT 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 326
PfeI GAWTC 3 cut(s) 162, 300, 407
PkrI GCNGC 8 cut(s) 26, 48, 63, 66, 77, 155, 251, 420
PleI GAGTC 3 cut(s) 6, 283, 325
PluTI GGCGCC 1 cut(s) 381
PpsI GAGTC 3 cut(s) 6, 283, 325
PspN4I GGNNCC 3 cut(s) 50, 148, 379
PspPI GGNCC 3 cut(s) 147, 251, 402
PstNI CAGNNNCTG 1 cut(s) 61
PsuI RGATCY 1 cut(s) 30
PteI GCGCGC 1 cut(s) 172
RruI TCGCGA 1 cut(s) 260
RsaI GTAC 2 cut(s) 182, 233
RsaNI GTAC 2 cut(s) 181, 232
SalI GTCGAC 2 cut(s) 328, 347
SatI GCNGC 8 cut(s) 25, 47, 62, 65, 76, 154, 250, 419
Sau3AI GATC 2 cut(s) 30, 261
Sau96I GGNCC 3 cut(s) 147, 251, 402
SchI GAGTC 3 cut(s) 6, 284, 326
ScrFI CCNGG 2 cut(s) 334, 382
SetI ASST 6 cut(s) 92, 132, 210, 294, 348, 375
SfoI GGCGCC 1 cut(s) 379
SgrDI CGTCGACG 1 cut(s) 347
SinI GGWCC 1 cut(s) 147
Sse9I AATT 2 cut(s) 395, 444
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 9 cut(s) 34, 46, 64, 67, 154, 249, 254, 310, 416
SspDI GGCGCC 1 cut(s) 377
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 332, 380
TaiI ACGT 4 cut(s) 92, 132, 348, 375
TaqI TCGA 5 cut(s) 242, 285, 329, 348, 412
TasI AATT 2 cut(s) 395, 444
TauI GCSGC 4 cut(s) 49, 67, 156, 252
TfiI GAWTC 3 cut(s) 162, 300, 407
TscAI CASTG 1 cut(s) 127
TseI GCWGC 4 cut(s) 24, 61, 75, 418
TspDTI ATGAA 1 cut(s) 292
TspRI CASTG 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 147
XmiI GTMKAC 2 cut(s) 329, 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.