pycom08g01350

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003608
Physical Location & Seq
Reverse (-)
1029397 .. 1029993
597 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01350.1

Sequence Viewer

Length: 597 bp
ATGGTACTTTCAAAACCCCTCCTCTTTATAACTCCCTCTCGGCTCAGAAGCACAATCCCCCAAATCTTTCATTCAGCGCCTTATATTTTCCAGCGAACCAAACAGAACTCAGAAGTCTCTCATTTTCCTTCACTTTCCCAGAAAATGTCAGACGGACTCGGAAAATGCAGCAAGATCCACCACATTGTGCGGCTCCGCCAGTTGCTGCGGCGGTGGCGCAGCAAGGCCTGCACGTCCACCAAGCGCATACCCTCCGATGTTCCCGCTGGACACGTCGCAGTCTGCGTGGGCACCAGCTGCACCAGATTCGTGGTGCGCGCGTCGTACTTGAACCACCCGGTCTTCAAAAAGCTCCTTGTGCAAGCCGAGGAGGAGTACGGCTTCTCGAACAGCGGCCCGCTCGCGATCCCCTGCGACGAGTCGCTGTTCGAGGAGGTTCTCCGATTCATTTCCCGCTCCGAGTCGCGTAAGTCGACCCGGTTAGTCAACGTCGACGACTTCCAGAAATACTGCCACGTTGGTGCCCGGAGTAACCTCGAACTGTGGGCCGATTCTCGACCGCTGCTTCGTGGATTTTCCGAGAAGACAATTCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

199

Amino Acids

22.59

Weight (kDa)

10.03

Isoelectric Point (pI)

66.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 64 - 150 3.5e-29 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
AccB1I GGYRCC 2 cut(s) 290, 521
AccBSI CCGCTC 2 cut(s) 400, 456
AccI GTMKAC 2 cut(s) 473, 492
AccII CGCG 4 cut(s) 318, 320, 404, 466
AciI CCGC 9 cut(s) 190, 196, 208, 211, 264, 393, 398, 454, 560
AclWI GGATC 2 cut(s) 169, 400
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 3 cut(s) 6, 326, 377
AflIII ACRYGT 1 cut(s) 271
AgsI TTSAA 3 cut(s) 12, 331, 346
AjiI CACGTC 2 cut(s) 234, 274
AleI CACNNNNGTG 1 cut(s) 519
AluBI AGCT 2 cut(s) 297, 352
AluI AGCT 2 cut(s) 297, 352
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 2 cut(s) 169, 400
AlwNI CAGNNNCTG 1 cut(s) 205
AoxI GGCC 3 cut(s) 225, 394, 546
ApeKI GCWGC 5 cut(s) 168, 205, 219, 297, 562
ArsI GACNNNNNNTTYG 2 cut(s) 549, 581
AspLEI GCGC 5 cut(s) 79, 219, 246, 318, 320
AspS9I GGNCC 2 cut(s) 395, 546
AsuC2I CCSGG 3 cut(s) 338, 478, 526
BaeGI GKGCMC 2 cut(s) 293, 526
BanI GGYRCC 2 cut(s) 290, 521
BbsI GAAGAC 2 cut(s) 334, 590
BbvI GCAGC 5 cut(s) 180, 192, 231, 284, 549
BceAI ACGGC 1 cut(s) 394
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BcnI CCSGG 3 cut(s) 338, 478, 526
BcoDI GTCTC 1 cut(s) 121
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 8 cut(s) 169, 191, 206, 209, 220, 298, 394, 563
BlsI GCNGC 8 cut(s) 170, 192, 207, 210, 221, 299, 395, 564
Bme1390I CCNGG 3 cut(s) 338, 478, 526
BmgBI CACGTC 2 cut(s) 234, 274
BmgT120I GGNCC 2 cut(s) 395, 546
BmiI GGNNCC 3 cut(s) 194, 292, 523
BmrFI CCNGG 3 cut(s) 338, 478, 526
BpiI GAAGAC 2 cut(s) 334, 590
BpuMI CCSGG 3 cut(s) 338, 478, 526
BsaJI CCNNGG 1 cut(s) 366
Bse1I ACTGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 58, 123
BseNI ACTGG 1 cut(s) 199
BsePI GCGCGC 1 cut(s) 316
BseRI GAGGAG 4 cut(s) 11, 383, 386, 446
BseSI GKGCMC 2 cut(s) 293, 526
BseXI GCAGC 5 cut(s) 180, 192, 231, 284, 549
BsgI GTGCAG 2 cut(s) 214, 283
Bsh1236I CGCG 4 cut(s) 318, 320, 404, 466
Bsh1285I CGRYCG 1 cut(s) 560
BshFI GGCC 3 cut(s) 227, 396, 548
BshNI GGYRCC 2 cut(s) 290, 521
BsiEI CGRYCG 1 cut(s) 560
BsiSI CCGG 3 cut(s) 338, 478, 526
BsmAI GTCTC 1 cut(s) 121
BsnI GGCC 3 cut(s) 227, 396, 548
Bsp1286I GDGCHC 2 cut(s) 293, 526
Bsp143I GATC 2 cut(s) 174, 405
Bsp68I TCGCGA 1 cut(s) 404
BspACI CCGC 9 cut(s) 190, 196, 208, 211, 264, 393, 398, 454, 560
BspANI GGCC 3 cut(s) 227, 396, 548
BspCNI CTCAG 2 cut(s) 57, 122
BspFNI CGCG 4 cut(s) 318, 320, 404, 466
BspLI GGNNCC 3 cut(s) 194, 292, 523
BspPI GGATC 2 cut(s) 169, 400
BspT107I GGYRCC 2 cut(s) 290, 521
BsrBI CCGCTC 2 cut(s) 400, 456
BsrI ACTGG 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 366
BssHII GCGCGC 1 cut(s) 316
BssMI GATC 2 cut(s) 174, 405
Bst4CI ACNGT 1 cut(s) 543
BstAPI GCANNNNNTGC 2 cut(s) 228, 297
BstC8I GCNNGC 5 cut(s) 229, 318, 363, 398, 402
BstDEI CTNAG 2 cut(s) 44, 109
BstFNI CGCG 4 cut(s) 318, 320, 404, 466
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 5 cut(s) 79, 219, 246, 318, 320
BstKTI GATC 2 cut(s) 177, 408
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 2 cut(s) 174, 405
BstMCI CGRYCG 1 cut(s) 560
BstMWI GCNNNNNNNGC 4 cut(s) 214, 228, 297, 358
BstSCI CCNGG 3 cut(s) 336, 476, 524
BstSLI GKGCMC 2 cut(s) 293, 526
BstUI CGCG 4 cut(s) 318, 320, 404, 466
BstV1I GCAGC 5 cut(s) 180, 192, 231, 284, 549
BstV2I GAAGAC 2 cut(s) 334, 590
BstX2I RGATCY 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 3 cut(s) 227, 396, 548
BtrI CACGTC 2 cut(s) 234, 274
BtuMI TCGCGA 1 cut(s) 404
Cac8I GCNNGC 5 cut(s) 229, 318, 363, 398, 402
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 5 cut(s) 79, 219, 246, 318, 320
Cfr13I GGNCC 2 cut(s) 395, 546
CseI GACGC 1 cut(s) 309
Csp6I GTAC 3 cut(s) 5, 325, 376
CviJI RGCY 9 cut(s) 43, 193, 227, 297, 352, 365, 381, 396, 548
CviKI_1 RGCY 9 cut(s) 43, 193, 227, 297, 352, 365, 381, 396, 548
CviQI GTAC 3 cut(s) 5, 325, 376
DdeI CTNAG 2 cut(s) 44, 109
DpnI GATC 2 cut(s) 176, 407
DpnII GATC 2 cut(s) 174, 405
DraIII CACNNNGTG 1 cut(s) 187
EciI GGCGGA 1 cut(s) 185
Eco147I AGGCCT 1 cut(s) 227
FaiI YATR 3 cut(s) 29, 84, 248
FauI CCCGC 3 cut(s) 271, 405, 461
FblI GTMKAC 2 cut(s) 473, 492
Fnu4HI GCNGC 8 cut(s) 169, 191, 206, 209, 220, 298, 394, 563
Fsp4HI GCNGC 8 cut(s) 169, 191, 206, 209, 220, 298, 394, 563
GlaI GCGC 5 cut(s) 78, 218, 245, 317, 319
GluI GCNGC 8 cut(s) 169, 191, 206, 209, 220, 298, 394, 563
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 3 cut(s) 227, 396, 548
HapII CCGG 3 cut(s) 338, 478, 526
HgaI GACGC 1 cut(s) 309
HhaI GCGC 5 cut(s) 79, 219, 246, 318, 320
Hin6I GCGC 5 cut(s) 77, 217, 244, 316, 318
HinP1I GCGC 5 cut(s) 77, 217, 244, 316, 318
HincII GTYRAC 3 cut(s) 474, 487, 493
HindII GTYRAC 3 cut(s) 474, 487, 493
HinfI GANTC 6 cut(s) 156, 306, 419, 444, 461, 551
HpaII CCGG 3 cut(s) 338, 478, 526
Hpy166II GTNNAC 4 cut(s) 237, 474, 487, 493
Hpy188I TCNGA 8 cut(s) 47, 112, 151, 161, 256, 443, 460, 580
Hpy188III TCNNGA 4 cut(s) 385, 403, 502, 555
Hpy8I GTNNAC 4 cut(s) 237, 474, 487, 493
Hpy99I CGWCG 5 cut(s) 278, 325, 419, 494, 497
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 1 cut(s) 543
HpyCH4IV ACGT 4 cut(s) 233, 273, 489, 516
HpyCH4V TGCA 4 cut(s) 168, 231, 300, 361
HpyF10VI GCNNNNNNNGC 4 cut(s) 214, 228, 297, 358
HpyF3I CTNAG 2 cut(s) 44, 109
HpySE526I ACGT 4 cut(s) 233, 273, 489, 516
HspAI GCGC 5 cut(s) 77, 217, 244, 316, 318
Kzo9I GATC 2 cut(s) 174, 405
LmnI GCTCC 3 cut(s) 198, 357, 461
Lsp1109I GCAGC 5 cut(s) 180, 192, 231, 284, 549
MaeII ACGT 4 cut(s) 233, 273, 489, 516
MaeIII GTNAC 1 cut(s) 530
MalI GATC 2 cut(s) 176, 407
MbiI CCGCTC 2 cut(s) 400, 456
MboI GATC 2 cut(s) 174, 405
MboII GAAGA 2 cut(s) 334, 595
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 2 cut(s) 293, 526
MluCI AATT 1 cut(s) 588
MlyI GAGTC 3 cut(s) 150, 428, 470
MnlI CCTC 9 cut(s) 29, 32, 46, 262, 361, 364, 424, 427, 545
MslI CAYNNNNRTG 1 cut(s) 519
MspA1I CMGCKG 4 cut(s) 266, 297, 393, 562
MspI CCGG 3 cut(s) 338, 478, 526
MspR9I CCNGG 3 cut(s) 338, 478, 526
MvnI CGCG 4 cut(s) 318, 320, 404, 466
MwoI GCNNNNNNNGC 4 cut(s) 214, 228, 297, 358
NciI CCSGG 3 cut(s) 338, 478, 526
NdeII GATC 2 cut(s) 174, 405
NlaIV GGNNCC 3 cut(s) 194, 292, 523
NmeAIII GCCGAG 2 cut(s) 19, 391
NruI TCGCGA 1 cut(s) 404
OliI CACNNNNGTG 1 cut(s) 519
PauI GCGCGC 1 cut(s) 316
PceI AGGCCT 1 cut(s) 227
PcsI WCGNNNNNNNCGW 2 cut(s) 282, 470
PfeI GAWTC 3 cut(s) 306, 444, 551
PkrI GCNGC 8 cut(s) 170, 192, 207, 210, 221, 299, 395, 564
PleI GAGTC 3 cut(s) 150, 427, 469
PpsI GAGTC 3 cut(s) 150, 427, 469
PsiI TTATAA 1 cut(s) 29
PspN4I GGNNCC 3 cut(s) 194, 292, 523
PspPI GGNCC 2 cut(s) 395, 546
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 174
PteI GCGCGC 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 297
RruI TCGCGA 1 cut(s) 404
RsaI GTAC 3 cut(s) 6, 326, 377
RsaNI GTAC 3 cut(s) 5, 325, 376
RseI CAYNNNNRTG 1 cut(s) 519
SalI GTCGAC 2 cut(s) 472, 491
SatI GCNGC 8 cut(s) 169, 191, 206, 209, 220, 298, 394, 563
Sau3AI GATC 2 cut(s) 174, 405
Sau96I GGNCC 2 cut(s) 395, 546
SchI GAGTC 3 cut(s) 150, 428, 470
ScrFI CCNGG 3 cut(s) 338, 478, 526
SduI GDGCHC 2 cut(s) 293, 526
SetI ASST 8 cut(s) 236, 276, 299, 354, 438, 492, 519, 537
SgrDI CGTCGACG 1 cut(s) 491
SmiMI CAYNNNNRTG 1 cut(s) 519
Sse9I AATT 1 cut(s) 588
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 9 cut(s) 190, 196, 208, 211, 264, 393, 398, 454, 560
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 3 cut(s) 336, 476, 524
TaaI ACNGT 1 cut(s) 543
TaiI ACGT 4 cut(s) 236, 276, 492, 519
TaqI TCGA 6 cut(s) 386, 429, 473, 492, 537, 556
TasI AATT 1 cut(s) 588
TauI GCSGC 3 cut(s) 193, 211, 396
TfiI GAWTC 3 cut(s) 306, 444, 551
TseI GCWGC 5 cut(s) 168, 205, 219, 297, 562
TspDTI ATGAA 2 cut(s) 59, 436
TspGWI ACGGA 1 cut(s) 168
XmiI GTMKAC 2 cut(s) 473, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.