MD15G1014300.v1.1

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003608
Physical Location & Seq
Reverse (-)
821989 .. 822441
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1014300.v1.1.491

Sequence Viewer

Length: 453 bp
ATGTCAGCCGGACTCGGAAAATGCAGCAAGATCCGCCACATTGTGCGGCTCCGCCAGTTGCTGCGGCGGTGGCGCAGCAAGGCCTGCACGTCCGCCAAGCTCATACCCTCCGATGTTCCCGCAGGACACGTGGCCGTCTGCGTGGGCACCAGCTGCACCCGATTCGTGGTGCGCGCGTCGTACCTGAACCACCCCGTCTTCAAGAAGCTCTTAGTGCAAGCCGAGGAGGAGTACGGCTTCTCGAACAGCGGTCCGCTTGCGATCCCCTGCGACGAGTCCCAGTTCGAGGAGGTACTTCGGTTCATTTCCCGGTCCGAGTCGGGTAACTCGACCCGGTTAGTCAACATCGACGATTTCCACAGATACTGCCACGTCGGCGTCCGGAGTAACCTCGATTTGTGGGCCGATTCTCGACCGTTGCTTCGTGGACTTTCCGAGAAGACAATTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

151

Amino Acids

16.92

Weight (kDa)

9.5

Isoelectric Point (pI)

68.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 16 - 102 4.8e-28 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 146
AccII CGCG 2 cut(s) 174, 176
AccIII TCCGGA 1 cut(s) 381
AciI CCGC 9 cut(s) 34, 46, 52, 64, 67, 93, 120, 249, 254
AclWI GGATC 2 cut(s) 25, 256
AcoI YGGCCR 1 cut(s) 132
AcvI CACGTG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 378
AdeI CACNNNGTG 1 cut(s) 43
AfaI GTAC 3 cut(s) 182, 233, 294
AfiI CCNNNNNNNGG 2 cut(s) 166, 286
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 1 cut(s) 202
AjiI CACGTC 2 cut(s) 90, 373
AluBI AGCT 3 cut(s) 100, 153, 208
AluI AGCT 3 cut(s) 100, 153, 208
AlwI GGATC 2 cut(s) 25, 256
AlwNI CAGNNNCTG 2 cut(s) 61, 366
Aor13HI TCCGGA 1 cut(s) 381
AoxI GGCC 3 cut(s) 81, 132, 402
ApeKI GCWGC 4 cut(s) 24, 61, 75, 153
ArsI GACNNNNNNTTYG 2 cut(s) 405, 437
AspLEI GCGC 3 cut(s) 75, 174, 176
AspS9I GGNCC 3 cut(s) 251, 312, 402
AsuC2I CCSGG 2 cut(s) 310, 334
AvaII GGWCC 2 cut(s) 251, 312
BaeGI GKGCMC 1 cut(s) 149
BaeI ACNNNNGTAYC 2 cut(s) 284, 317
BanI GGYRCC 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 130
BbsI GAAGAC 2 cut(s) 190, 446
BbvI GCAGC 4 cut(s) 36, 48, 87, 140
BceAI ACGGC 2 cut(s) 119, 250
BcgI CGANNNNNNTGC 2 cut(s) 145, 179
BcnI CCSGG 2 cut(s) 310, 334
BglI GCCNNNNNGGC 1 cut(s) 375
BisI GCNGC 6 cut(s) 25, 47, 62, 65, 76, 154
BlsI GCNGC 6 cut(s) 26, 48, 63, 66, 77, 155
Bme1390I CCNGG 2 cut(s) 310, 334
Bme18I GGWCC 2 cut(s) 251, 312
BmgBI CACGTC 2 cut(s) 90, 373
BmgT120I GGNCC 3 cut(s) 251, 312, 402
BmiI GGNNCC 2 cut(s) 50, 148
BmrFI CCNGG 2 cut(s) 310, 334
BmrI ACTGGG 1 cut(s) 274
BmuI ACTGGG 1 cut(s) 274
BpiI GAAGAC 2 cut(s) 190, 446
BpuMI CCSGG 2 cut(s) 310, 334
BsaAI YACGTR 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 381
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 286
Bse1I ACTGG 2 cut(s) 55, 280
BseAI TCCGGA 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 166, 286
BseNI ACTGG 2 cut(s) 55, 280
BsePI GCGCGC 1 cut(s) 172
BseRI GAGGAG 3 cut(s) 239, 242, 302
BseSI GKGCMC 1 cut(s) 149
BseXI GCAGC 4 cut(s) 36, 48, 87, 140
BsgI GTGCAG 2 cut(s) 70, 139
Bsh1236I CGCG 2 cut(s) 174, 176
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 3 cut(s) 83, 134, 404
BshNI GGYRCC 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 416
BsiSI CCGG 4 cut(s) 9, 310, 334, 382
BslFI GGGAC 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 166, 286
BsmFI GGGAC 1 cut(s) 262
BsnI GGCC 3 cut(s) 83, 134, 404
Bsp1286I GDGCHC 1 cut(s) 149
Bsp13I TCCGGA 1 cut(s) 381
Bsp143I GATC 2 cut(s) 30, 261
BspACI CCGC 9 cut(s) 34, 46, 52, 64, 67, 93, 120, 249, 254
BspANI GGCC 3 cut(s) 83, 134, 404
BspEI TCCGGA 1 cut(s) 381
BspFNI CGCG 2 cut(s) 174, 176
BspLI GGNNCC 2 cut(s) 50, 148
BspPI GGATC 2 cut(s) 25, 256
BspT107I GGYRCC 1 cut(s) 146
BsrI ACTGG 2 cut(s) 55, 280
BssECI CCNNGG 1 cut(s) 222
BssHII GCGCGC 1 cut(s) 172
BssMI GATC 2 cut(s) 30, 261
BssNI GRCGYC 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 417
BstACI GRCGYC 1 cut(s) 378
BstAPI GCANNNNNTGC 2 cut(s) 84, 153
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 4 cut(s) 85, 174, 219, 258
BstDEI CTNAG 1 cut(s) 211
BstFNI CGCG 2 cut(s) 174, 176
BstHHI GCGC 3 cut(s) 75, 174, 176
BstKTI GATC 2 cut(s) 33, 264
BstMBI GATC 2 cut(s) 30, 261
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 6 cut(s) 33, 70, 84, 153, 214, 375
BstSCI CCNGG 2 cut(s) 308, 332
BstSLI GKGCMC 1 cut(s) 149
BstUI CGCG 2 cut(s) 174, 176
BstV1I GCAGC 4 cut(s) 36, 48, 87, 140
BstV2I GAAGAC 2 cut(s) 190, 446
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BsuRI GGCC 3 cut(s) 83, 134, 404
BtrI CACGTC 2 cut(s) 90, 373
Cac8I GCNNGC 4 cut(s) 85, 174, 219, 258
CaiI CAGNNNCTG 2 cut(s) 61, 366
CfoI GCGC 3 cut(s) 75, 174, 176
Cfr13I GGNCC 3 cut(s) 251, 312, 402
CpoI CGGWCCG 2 cut(s) 251, 312
CseI GACGC 2 cut(s) 165, 367
Csp6I GTAC 3 cut(s) 181, 232, 293
CspI CGGWCCG 2 cut(s) 251, 312
CviQI GTAC 3 cut(s) 181, 232, 293
DdeI CTNAG 1 cut(s) 211
DpnI GATC 2 cut(s) 32, 263
DpnII GATC 2 cut(s) 30, 261
DraIII CACNNNGTG 1 cut(s) 43
EaeI YGGCCR 1 cut(s) 132
EciI GGCGGA 3 cut(s) 23, 41, 82
Eco147I AGGCCT 1 cut(s) 83
Eco47I GGWCC 2 cut(s) 251, 312
Eco72I CACGTG 1 cut(s) 130
FaiI YATR 1 cut(s) 104
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 262
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 6 cut(s) 25, 47, 62, 65, 76, 154
Fsp4HI GCNGC 6 cut(s) 25, 47, 62, 65, 76, 154
GlaI GCGC 3 cut(s) 74, 173, 175
GluI GCNGC 6 cut(s) 25, 47, 62, 65, 76, 154
HaeIII GGCC 3 cut(s) 83, 134, 404
HapII CCGG 4 cut(s) 9, 310, 334, 382
HgaI GACGC 2 cut(s) 165, 367
HhaI GCGC 3 cut(s) 75, 174, 176
Hin1I GRCGYC 1 cut(s) 378
Hin6I GCGC 3 cut(s) 73, 172, 174
HinP1I GCGC 3 cut(s) 73, 172, 174
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 5 cut(s) 12, 162, 275, 317, 407
HpaII CCGG 4 cut(s) 9, 310, 334, 382
Hpy166II GTNNAC 2 cut(s) 343, 428
Hpy188I TCNGA 4 cut(s) 17, 112, 316, 436
Hpy188III TCNNGA 4 cut(s) 202, 241, 382, 411
Hpy8I GTNNAC 2 cut(s) 343, 428
Hpy99I CGWCG 4 cut(s) 181, 275, 353, 377
HpyCH4III ACNGT 1 cut(s) 417
HpyCH4IV ACGT 3 cut(s) 89, 129, 372
HpyCH4V TGCA 4 cut(s) 24, 87, 156, 217
HpyF10VI GCNNNNNNNGC 6 cut(s) 33, 70, 84, 153, 214, 375
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 3 cut(s) 89, 129, 372
Hsp92I GRCGYC 1 cut(s) 378
HspAI GCGC 3 cut(s) 73, 172, 174
Kpn2I TCCGGA 1 cut(s) 381
Kzo9I GATC 2 cut(s) 30, 261
LmnI GCTCC 1 cut(s) 54
Lsp1109I GCAGC 4 cut(s) 36, 48, 87, 140
MaeII ACGT 3 cut(s) 89, 129, 372
MaeIII GTNAC 2 cut(s) 323, 386
MalI GATC 2 cut(s) 32, 263
MboI GATC 2 cut(s) 30, 261
MboII GAAGA 2 cut(s) 190, 451
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 149
MluCI AATT 1 cut(s) 444
MlyI GAGTC 3 cut(s) 6, 284, 326
MnlI CCTC 6 cut(s) 118, 217, 220, 280, 283, 401
MroI TCCGGA 1 cut(s) 381
MspA1I CMGCKG 2 cut(s) 153, 249
MspI CCGG 4 cut(s) 9, 310, 334, 382
MspR9I CCNGG 2 cut(s) 310, 334
MvnI CGCG 2 cut(s) 174, 176
MwoI GCNNNNNNNGC 6 cut(s) 33, 70, 84, 153, 214, 375
NciI CCSGG 2 cut(s) 310, 334
NdeII GATC 2 cut(s) 30, 261
NlaIV GGNNCC 2 cut(s) 50, 148
NmeAIII GCCGAG 1 cut(s) 247
PauI GCGCGC 1 cut(s) 172
PceI AGGCCT 1 cut(s) 83
PcsI WCGNNNNNNNCGW 1 cut(s) 326
PfeI GAWTC 2 cut(s) 162, 407
PkrI GCNGC 6 cut(s) 26, 48, 63, 66, 77, 155
PleI GAGTC 3 cut(s) 6, 283, 325
PmaCI CACGTG 1 cut(s) 130
PmlI CACGTG 1 cut(s) 130
PpsI GAGTC 3 cut(s) 6, 283, 325
Ppu21I YACGTR 1 cut(s) 130
PspCI CACGTG 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 50, 148
PspPI GGNCC 3 cut(s) 251, 312, 402
PstNI CAGNNNCTG 2 cut(s) 61, 366
PsuI RGATCY 1 cut(s) 30
PteI GCGCGC 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 153
RsaI GTAC 3 cut(s) 182, 233, 294
RsaNI GTAC 3 cut(s) 181, 232, 293
Rsr2I CGGWCCG 2 cut(s) 251, 312
RsrII CGGWCCG 2 cut(s) 251, 312
SatI GCNGC 6 cut(s) 25, 47, 62, 65, 76, 154
Sau3AI GATC 2 cut(s) 30, 261
Sau96I GGNCC 3 cut(s) 251, 312, 402
SchI GAGTC 3 cut(s) 6, 284, 326
ScrFI CCNGG 2 cut(s) 310, 334
SduI GDGCHC 1 cut(s) 149
SetI ASST 9 cut(s) 92, 102, 132, 155, 186, 210, 294, 375, 393
SinI GGWCC 2 cut(s) 251, 312
Sse9I AATT 1 cut(s) 444
SseBI AGGCCT 1 cut(s) 83
SsiI CCGC 9 cut(s) 34, 46, 52, 64, 67, 93, 120, 249, 254
StuI AGGCCT 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 308, 332
TaaI ACNGT 1 cut(s) 417
TaiI ACGT 3 cut(s) 92, 132, 375
TaqI TCGA 6 cut(s) 242, 285, 329, 348, 393, 412
TasI AATT 1 cut(s) 444
TauI GCSGC 2 cut(s) 49, 67
TfiI GAWTC 2 cut(s) 162, 407
TseI GCWGC 4 cut(s) 24, 61, 75, 153
TspDTI ATGAA 1 cut(s) 292
VpaK11BI GGWCC 2 cut(s) 251, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.