MD10G1061400.v1.1

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0003608
Physical Location & Seq
Reverse (-)
8286787 .. 8287227
441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1061400.v1.1.491

Sequence Viewer

Length: 441 bp
ATGTCACCGGCGATCAGCAAATGCAACAAGCTCCGACGCATTGTCAGAATCCGTCAAATGCTACAACGCTGGCACAAGAAGGCGCGCCTCACGGCAGCACGCGCGCCGTCCGACGTTCCCTCAGGACACGTTGCGGTCTGCGTGGGGAGCAACTGCAAGCGGTTTATCGTGCGCGCGACGTACCTGAACCACCCTATCTTCCAGAAGCTTCTAGTAGAGGCCGAGGAGGAGTACGGCTTCGCCAACCAGGGCCCCTTGGCCATCCCATGCGACGAGTCGTTTTTCGAAGAAGTTCTTCGGGTCCTGGCCCGGTCCGAGTCTGGTAACTCCGGGCGATTCTTGAGCGTGGAGGAACTCCAGAGACGCTGCCACGGGGATGCTTTGAACCAGCTTGAGTATTTGGGTGAATCTCGGCCGTTGCTTCGCGCGCGGGTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

147

Amino Acids

16.53

Weight (kDa)

9.66

Isoelectric Point (pI)

66.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 16 - 102 7e-30 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 8 cut(s) 85, 102, 104, 174, 176, 426, 428, 430
AciI CCGC 3 cut(s) 134, 160, 430
AcoI YGGCCR 2 cut(s) 258, 413
AfaI GTAC 2 cut(s) 182, 233
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 1 cut(s) 385
AhdI GACNNNNNGTC 1 cut(s) 41
AjnI CCWGG 2 cut(s) 246, 303
AluBI AGCT 3 cut(s) 31, 208, 391
AluI AGCT 3 cut(s) 31, 208, 391
Alw26I GTCTC 1 cut(s) 355
AoxI GGCC 5 cut(s) 219, 250, 258, 306, 413
ApaI GGGCCC 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 95, 366
AscI GGCGCGCC 1 cut(s) 83
Asp700I GAANNNNTTC 2 cut(s) 291, 294
AspLEI GCGC 8 cut(s) 85, 87, 104, 106, 174, 176, 428, 430
AspS9I GGNCC 5 cut(s) 250, 251, 301, 307, 312
AsuC2I CCSGG 2 cut(s) 310, 331
AsuHPI GGTGA 1 cut(s) 416
AsuII TTCGAA 1 cut(s) 285
AvaII GGWCC 2 cut(s) 301, 312
AxyI CCTNAGG 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 254
BalI TGGCCA 1 cut(s) 260
BanII GRGCYC 1 cut(s) 254
BbvI GCAGC 2 cut(s) 107, 353
BccI CCATC 1 cut(s) 269
BceAI ACGGC 4 cut(s) 91, 108, 250, 400
BciT130I CCWGG 2 cut(s) 248, 305
BcnI CCSGG 2 cut(s) 310, 331
BcoDI GTCTC 1 cut(s) 355
BfaI CTAG 1 cut(s) 212
BisI GCNGC 2 cut(s) 96, 367
BlsI GCNGC 2 cut(s) 97, 368
Bme1390I CCNGG 4 cut(s) 248, 305, 310, 331
Bme18I GGWCC 2 cut(s) 301, 312
BmeRI GACNNNNNGTC 1 cut(s) 41
BmgT120I GGNCC 5 cut(s) 250, 251, 301, 307, 312
BmiI GGNNCC 3 cut(s) 252, 253, 302
BmrFI CCNGG 4 cut(s) 248, 305, 310, 331
BmsI GCATC 1 cut(s) 367
BpmI CTGGAG 1 cut(s) 341
Bpu14I TTCGAA 1 cut(s) 285
BpuEI CTTGAG 2 cut(s) 361, 413
BpuMI CCSGG 2 cut(s) 310, 331
BsaJI CCNNGG 4 cut(s) 222, 247, 255, 370
Bse118I RCCGGY 1 cut(s) 7
Bse21I CCTNAGG 1 cut(s) 121
BseBI CCWGG 2 cut(s) 248, 305
BseDI CCNNGG 4 cut(s) 222, 247, 255, 370
BseGI GGATG 2 cut(s) 261, 382
BseMII CTCAG 1 cut(s) 135
BsePI GCGCGC 4 cut(s) 83, 102, 172, 426
BseRI GAGGAG 2 cut(s) 239, 242
BseSI GKGCMC 1 cut(s) 254
BseX3I CGGCCG 1 cut(s) 413
BseXI GCAGC 2 cut(s) 107, 353
Bsh1236I CGCG 8 cut(s) 85, 102, 104, 174, 176, 426, 428, 430
Bsh1285I CGRYCG 1 cut(s) 416
BshFI GGCC 5 cut(s) 221, 252, 260, 308, 415
BsiEI CGRYCG 1 cut(s) 416
BsiSI CCGG 3 cut(s) 8, 310, 330
BsmAI GTCTC 1 cut(s) 355
BsmBI CGTCTC 1 cut(s) 355
BsnI GGCC 5 cut(s) 221, 252, 260, 308, 415
Bsp119I TTCGAA 1 cut(s) 285
Bsp120I GGGCCC 1 cut(s) 250
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 1 cut(s) 12
BspACI CCGC 3 cut(s) 134, 160, 430
BspANI GGCC 5 cut(s) 221, 252, 260, 308, 415
BspCNI CTCAG 1 cut(s) 134
BspFNI CGCG 8 cut(s) 85, 102, 104, 174, 176, 426, 428, 430
BspLI GGNNCC 3 cut(s) 252, 253, 302
BspT104I TTCGAA 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 7
BssAI RCCGGY 1 cut(s) 7
BssECI CCNNGG 4 cut(s) 222, 247, 255, 370
BssHII GCGCGC 4 cut(s) 83, 102, 172, 426
BssMI GATC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 248, 305
BstBI TTCGAA 1 cut(s) 285
BstC8I GCNNGC 7 cut(s) 71, 85, 100, 104, 158, 174, 428
BstDEI CTNAG 1 cut(s) 121
BstDSI CCRYGG 1 cut(s) 370
BstF5I GGATG 2 cut(s) 261, 382
BstFNI CGCG 8 cut(s) 85, 102, 104, 174, 176, 426, 428, 430
BstHHI GCGC 8 cut(s) 85, 87, 104, 106, 174, 176, 428, 430
BstKTI GATC 1 cut(s) 15
BstMAI GTCTC 1 cut(s) 355
BstMBI GATC 1 cut(s) 12
BstMCI CGRYCG 1 cut(s) 416
BstMWI GCNNNNNNNGC 3 cut(s) 101, 147, 427
BstNI CCWGG 2 cut(s) 248, 305
BstSCI CCNGG 4 cut(s) 246, 303, 308, 329
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 8 cut(s) 85, 102, 104, 174, 176, 426, 428, 430
BstV1I GCAGC 2 cut(s) 107, 353
BstZI CGGCCG 1 cut(s) 413
Bsu36I CCTNAGG 1 cut(s) 121
BsuRI GGCC 5 cut(s) 221, 252, 260, 308, 415
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 261, 382
Cac8I GCNNGC 7 cut(s) 71, 85, 100, 104, 158, 174, 428
CfoI GCGC 8 cut(s) 85, 87, 104, 106, 174, 176, 428, 430
Cfr10I RCCGGY 1 cut(s) 7
Cfr13I GGNCC 5 cut(s) 250, 251, 301, 307, 312
CpoI CGGWCCG 1 cut(s) 312
CseI GACGC 2 cut(s) 45, 372
Csp6I GTAC 2 cut(s) 181, 232
CspI CGGWCCG 1 cut(s) 312
CviAII CATG 1 cut(s) 267
CviQI GTAC 2 cut(s) 181, 232
DdeI CTNAG 1 cut(s) 121
DpnI GATC 1 cut(s) 14
DpnII GATC 1 cut(s) 12
DriI GACNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 2 cut(s) 258, 413
EagI CGGCCG 1 cut(s) 413
Eam1105I GACNNNNNGTC 1 cut(s) 41
EclXI CGGCCG 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 2 cut(s) 301, 312
Eco52I CGGCCG 1 cut(s) 413
Eco81I CCTNAGG 1 cut(s) 121
EcoO109I RGGNCCY 3 cut(s) 250, 251, 301
EcoRII CCWGG 2 cut(s) 246, 303
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 254
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 355
FaeI CATG 1 cut(s) 270
FaiI YATR 1 cut(s) 268
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 1 cut(s) 266
FauI CCCGC 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 96, 367
FokI GGATG 2 cut(s) 248, 389
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 2 cut(s) 96, 367
FspBI CTAG 1 cut(s) 212
GlaI GCGC 8 cut(s) 84, 86, 103, 105, 173, 175, 427, 429
GluI GCNGC 2 cut(s) 96, 367
GsuI CTGGAG 1 cut(s) 341
HaeIII GGCC 5 cut(s) 221, 252, 260, 308, 415
HapII CCGG 3 cut(s) 8, 310, 330
HgaI GACGC 2 cut(s) 45, 372
HhaI GCGC 8 cut(s) 85, 87, 104, 106, 174, 176, 428, 430
Hin1II CATG 1 cut(s) 270
Hin6I GCGC 8 cut(s) 83, 85, 102, 104, 172, 174, 426, 428
HinP1I GCGC 8 cut(s) 83, 85, 102, 104, 172, 174, 426, 428
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 5 cut(s) 48, 275, 317, 336, 407
HpaII CCGG 3 cut(s) 8, 310, 330
HphI GGTGA 1 cut(s) 416
Hpy188I TCNGA 4 cut(s) 35, 47, 112, 316
Hpy188III TCNNGA 4 cut(s) 123, 202, 340, 358
Hpy99I CGWCG 4 cut(s) 39, 116, 181, 275
HpyAV CCTTC 1 cut(s) 73
HpyCH4IV ACGT 3 cut(s) 114, 129, 179
HpyCH4V TGCA 2 cut(s) 24, 156
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 147, 427
HpyF3I CTNAG 1 cut(s) 121
HpySE526I ACGT 3 cut(s) 114, 129, 179
Hsp92II CATG 1 cut(s) 270
HspAI GCGC 8 cut(s) 83, 85, 102, 104, 172, 174, 426, 428
Kzo9I GATC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 36, 147
Lsp1109I GCAGC 2 cut(s) 107, 353
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 212
MaeII ACGT 3 cut(s) 114, 129, 179
MaeIII GTNAC 2 cut(s) 3, 323
MalI GATC 1 cut(s) 14
MauBI CGCGCGCG 1 cut(s) 426
MboI GATC 1 cut(s) 12
MboII GAAGA 3 cut(s) 190, 287, 299
MhlI GDGCHC 1 cut(s) 254
MlsI TGGCCA 1 cut(s) 260
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 2 cut(s) 284, 326
MmeI TCCRAC 2 cut(s) 58, 135
MnlI CCTC 6 cut(s) 98, 130, 211, 217, 220, 343
Mox20I TGGCCA 1 cut(s) 260
MroXI GAANNNNTTC 2 cut(s) 291, 294
MscI TGGCCA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 260
MspI CCGG 3 cut(s) 8, 310, 330
MspR9I CCNGG 4 cut(s) 248, 305, 310, 331
MvaI CCWGG 2 cut(s) 248, 305
MvnI CGCG 8 cut(s) 85, 102, 104, 174, 176, 426, 428, 430
MwoI GCNNNNNNNGC 3 cut(s) 101, 147, 427
NciI CCSGG 2 cut(s) 310, 331
NdeII GATC 1 cut(s) 12
NlaIII CATG 1 cut(s) 270
NlaIV GGNNCC 3 cut(s) 252, 253, 302
NmeAIII GCCGAG 2 cut(s) 247, 391
NmuCI GTSAC 1 cut(s) 3
NspV TTCGAA 1 cut(s) 285
PalAI GGCGCGCC 1 cut(s) 83
PauI GCGCGC 4 cut(s) 83, 102, 172, 426
PdmI GAANNNNTTC 2 cut(s) 291, 294
PfeI GAWTC 3 cut(s) 48, 336, 407
PkrI GCNGC 2 cut(s) 97, 368
PleI GAGTC 2 cut(s) 283, 325
PpsI GAGTC 2 cut(s) 283, 325
PpuMI RGGWCCY 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 246, 303
PspGI CCWGG 2 cut(s) 246, 303
PspN4I GGNNCC 3 cut(s) 252, 253, 302
PspOMI GGGCCC 1 cut(s) 250
PspPI GGNCC 5 cut(s) 250, 251, 301, 307, 312
PspPPI RGGWCCY 1 cut(s) 301
PteI GCGCGC 4 cut(s) 83, 102, 172, 426
RsaI GTAC 2 cut(s) 182, 233
RsaNI GTAC 2 cut(s) 181, 232
RseI CAYNNNNRTG 1 cut(s) 375
Rsr2I CGGWCCG 1 cut(s) 312
RsrII CGGWCCG 1 cut(s) 312
SatI GCNGC 2 cut(s) 96, 367
Sau3AI GATC 1 cut(s) 12
Sau96I GGNCC 5 cut(s) 250, 251, 301, 307, 312
SchI GAGTC 2 cut(s) 284, 326
ScrFI CCNGG 4 cut(s) 248, 305, 310, 331
SduI GDGCHC 1 cut(s) 254
SetI ASST 7 cut(s) 33, 117, 132, 182, 186, 210, 393
SfaNI GCATC 1 cut(s) 367
SfuI TTCGAA 1 cut(s) 285
SgrAI CRCCGGYG 1 cut(s) 7
SgsI GGCGCGCC 1 cut(s) 83
SinI GGWCC 2 cut(s) 301, 312
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 2 cut(s) 340, 392
SmoI CTYRAG 2 cut(s) 340, 392
SsiI CCGC 3 cut(s) 134, 160, 430
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 4 cut(s) 246, 303, 308, 329
StyI CCWWGG 1 cut(s) 255
TaiI ACGT 3 cut(s) 117, 132, 182
TaqI TCGA 1 cut(s) 285
TfiI GAWTC 3 cut(s) 48, 336, 407
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 95, 366
Tsp45I GTSAC 1 cut(s) 3
TspGWI ACGGA 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 301, 312
XmnI GAANNNNTTC 2 cut(s) 291, 294
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.