pycom10g04730

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003608
Physical Location & Seq
Reverse (-)
4970218 .. 4970833
616 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04730.1

Sequence Viewer

Length: 549 bp
ATGGCCTTGACAACAACTTTAACAACAGCCACAATCCCACTCGTACCCCCATCATCGCCATTACCTCCTTTTAAGCAACTACCATTCTTTCCAACTCTTTCCGGCAATATGTCGCCGGCGATCAGCAAATGCAACAAACTTCGACGCATTGTTAGAATCCGTCAAATGCTACAACGCTGGCACAAGAAGGCCCGCCTCACGGCAGCTCGCGCGCCGTCCGACGTCCCCTCAGGACACGTTGCGGTCTGCGTGGGAAGCAGCTACAAGCGGTTTATCGTGCGCGCGACGTACCTGAACCACCCTATCTTCCAGAAGCTTCTAGTAGAGGCCGAGGAGGAGTACGGCTTCGCCAACAAGGGCCCCTTGGCCATCCCATGCGACGAGTCGTTTTTCGAAGAGGTTCTTCGGGTCCTGGCCCGGTCCGAGTCTGGTAACTCCGGGCGATTCTTGAACGTGGAGGATCTCCAGAGACGCTGCCACGGGGATGCTTTGAACCAGCTTGAGTATTTGGGTGAATCTGGGCCGTTGCTTCGCGCTTGGGTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

183

Amino Acids

20.29

Weight (kDa)

9.65

Isoelectric Point (pI)

67.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 52 - 138 4.3e-29 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
AccII CGCG 5 cut(s) 210, 212, 282, 284, 534
AciI CCGC 3 cut(s) 193, 242, 268
AclWI GGATC 1 cut(s) 468
AcoI YGGCCR 1 cut(s) 366
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 45, 290, 341
AfiI CCNNNNNNNGG 1 cut(s) 199
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 2 cut(s) 451, 493
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 4 cut(s) 206, 261, 316, 499
AluI AGCT 4 cut(s) 206, 261, 316, 499
Alw26I GTCTC 1 cut(s) 463
AlwI GGATC 1 cut(s) 468
AoxI GGCC 7 cut(s) 3, 189, 327, 358, 366, 414, 521
ApaI GGGCCC 1 cut(s) 362
ApeKI GCWGC 3 cut(s) 203, 258, 474
Asp700I GAANNNNTTC 1 cut(s) 399
AspLEI GCGC 5 cut(s) 212, 214, 282, 284, 536
AspS9I GGNCC 7 cut(s) 190, 358, 359, 409, 415, 420, 521
AsuC2I CCSGG 2 cut(s) 418, 439
AsuHPI GGTGA 1 cut(s) 524
AsuII TTCGAA 1 cut(s) 393
AvaII GGWCC 2 cut(s) 409, 420
AxyI CCTNAGG 1 cut(s) 229
BaeGI GKGCMC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 368
BanII GRGCYC 1 cut(s) 362
BbvI GCAGC 3 cut(s) 215, 270, 461
BccI CCATC 2 cut(s) 58, 377
BceAI ACGGC 4 cut(s) 199, 216, 358, 508
BciT130I CCWGG 1 cut(s) 413
BcnI CCSGG 2 cut(s) 418, 439
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 1 cut(s) 320
BisI GCNGC 3 cut(s) 204, 259, 475
BlsI GCNGC 3 cut(s) 205, 260, 476
Bme1390I CCNGG 3 cut(s) 413, 418, 439
Bme18I GGWCC 2 cut(s) 409, 420
BmgT120I GGNCC 7 cut(s) 190, 358, 359, 409, 415, 420, 521
BmiI GGNNCC 3 cut(s) 360, 361, 410
BmrFI CCNGG 3 cut(s) 413, 418, 439
BmsI GCATC 1 cut(s) 475
BpmI CTGGAG 1 cut(s) 449
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 2 cut(s) 418, 439
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 330, 363, 478
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse118I RCCGGY 1 cut(s) 115
Bse21I CCTNAGG 1 cut(s) 229
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 3 cut(s) 330, 363, 478
BseGI GGATG 2 cut(s) 369, 490
BseLI CCNNNNNNNGG 1 cut(s) 199
BseMII CTCAG 1 cut(s) 243
BsePI GCGCGC 2 cut(s) 210, 280
BseRI GAGGAG 2 cut(s) 347, 350
BseSI GKGCMC 1 cut(s) 362
BseXI GCAGC 3 cut(s) 215, 270, 461
Bsh1236I CGCG 5 cut(s) 210, 212, 282, 284, 534
BshFI GGCC 7 cut(s) 5, 191, 329, 360, 368, 416, 523
BsiSI CCGG 4 cut(s) 102, 116, 418, 438
BslFI GGGAC 1 cut(s) 209
BslI CCNNNNNNNGG 1 cut(s) 199
BsmAI GTCTC 1 cut(s) 463
BsmBI CGTCTC 1 cut(s) 463
BsmFI GGGAC 1 cut(s) 209
BsnI GGCC 7 cut(s) 5, 191, 329, 360, 368, 416, 523
Bsp119I TTCGAA 1 cut(s) 393
Bsp120I GGGCCC 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 2 cut(s) 120, 460
BspACI CCGC 3 cut(s) 193, 242, 268
BspANI GGCC 7 cut(s) 5, 191, 329, 360, 368, 416, 523
BspCNI CTCAG 1 cut(s) 242
BspFNI CGCG 5 cut(s) 210, 212, 282, 284, 534
BspLI GGNNCC 3 cut(s) 360, 361, 410
BspPI GGATC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 393
BsrFI RCCGGY 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 330, 363, 478
BssHII GCGCGC 2 cut(s) 210, 280
BssMI GATC 2 cut(s) 120, 460
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 1 cut(s) 413
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 6 cut(s) 117, 179, 193, 208, 212, 282
BstDEI CTNAG 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 478
BstF5I GGATG 2 cut(s) 369, 490
BstFNI CGCG 5 cut(s) 210, 212, 282, 284, 534
BstHHI GCGC 5 cut(s) 212, 214, 282, 284, 536
BstKTI GATC 2 cut(s) 123, 463
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 2 cut(s) 120, 460
BstMWI GCNNNNNNNGC 2 cut(s) 209, 255
BstNI CCWGG 1 cut(s) 413
BstSCI CCNGG 3 cut(s) 411, 416, 437
BstSLI GKGCMC 1 cut(s) 362
BstUI CGCG 5 cut(s) 210, 212, 282, 284, 534
BstV1I GCAGC 3 cut(s) 215, 270, 461
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
Bsu36I CCTNAGG 1 cut(s) 229
BsuRI GGCC 7 cut(s) 5, 191, 329, 360, 368, 416, 523
BtgI CCRYGG 1 cut(s) 478
BtgZI GCGATG 1 cut(s) 39
BtsCI GGATG 2 cut(s) 369, 490
Cac8I GCNNGC 6 cut(s) 117, 179, 193, 208, 212, 282
CfoI GCGC 5 cut(s) 212, 214, 282, 284, 536
Cfr10I RCCGGY 1 cut(s) 115
Cfr13I GGNCC 7 cut(s) 190, 358, 359, 409, 415, 420, 521
CpoI CGGWCCG 1 cut(s) 420
CseI GACGC 2 cut(s) 153, 480
Csp6I GTAC 3 cut(s) 44, 289, 340
CspI CGGWCCG 1 cut(s) 420
CviAII CATG 1 cut(s) 375
CviQI GTAC 3 cut(s) 44, 289, 340
DdeI CTNAG 1 cut(s) 229
DpnI GATC 2 cut(s) 122, 462
DpnII GATC 2 cut(s) 120, 460
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 2 cut(s) 409, 420
Eco81I CCTNAGG 1 cut(s) 229
EcoO109I RGGNCCY 3 cut(s) 358, 359, 409
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 463
FaeI CATG 1 cut(s) 378
FaiI YATR 2 cut(s) 110, 376
FalI AAGNNNNNCTT 4 cut(s) 347, 379, 387, 419
FaqI GGGAC 1 cut(s) 209
FatI CATG 1 cut(s) 374
FauI CCCGC 1 cut(s) 200
Fnu4HI GCNGC 3 cut(s) 204, 259, 475
FokI GGATG 2 cut(s) 356, 497
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 3 cut(s) 204, 259, 475
FspBI CTAG 1 cut(s) 320
GlaI GCGC 5 cut(s) 211, 213, 281, 283, 535
GluI GCNGC 3 cut(s) 204, 259, 475
GsuI CTGGAG 1 cut(s) 449
HaeIII GGCC 7 cut(s) 5, 191, 329, 360, 368, 416, 523
HapII CCGG 4 cut(s) 102, 116, 418, 438
HgaI GACGC 2 cut(s) 153, 480
HhaI GCGC 5 cut(s) 212, 214, 282, 284, 536
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 1 cut(s) 378
Hin6I GCGC 5 cut(s) 210, 212, 280, 282, 534
HinP1I GCGC 5 cut(s) 210, 212, 280, 282, 534
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 5 cut(s) 156, 383, 425, 444, 515
HpaII CCGG 4 cut(s) 102, 116, 418, 438
HphI GGTGA 1 cut(s) 524
Hpy188I TCNGA 2 cut(s) 220, 424
Hpy188III TCNNGA 4 cut(s) 231, 310, 448, 466
Hpy99I CGWCG 4 cut(s) 147, 224, 289, 383
HpyAV CCTTC 1 cut(s) 181
HpyCH4IV ACGT 4 cut(s) 222, 237, 287, 453
HpyCH4V TGCA 1 cut(s) 132
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 255
HpyF3I CTNAG 1 cut(s) 229
HpySE526I ACGT 4 cut(s) 222, 237, 287, 453
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 1 cut(s) 378
HspAI GCGC 5 cut(s) 210, 212, 280, 282, 534
KroI GCCGGC 1 cut(s) 115
KroNI GCCGGC 1 cut(s) 117
Kzo9I GATC 2 cut(s) 120, 460
Lsp1109I GCAGC 3 cut(s) 215, 270, 461
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 320
MaeII ACGT 4 cut(s) 222, 237, 287, 453
MaeIII GTNAC 1 cut(s) 431
MalI GATC 2 cut(s) 122, 462
MboI GATC 2 cut(s) 120, 460
MboII GAAGA 3 cut(s) 298, 395, 407
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 362
MlsI TGGCCA 1 cut(s) 368
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 2 cut(s) 392, 434
MmeI TCCRAC 2 cut(s) 116, 243
MnlI CCTC 8 cut(s) 75, 206, 238, 319, 325, 328, 391, 451
Mox20I TGGCCA 1 cut(s) 368
MreI CGCCGGCG 1 cut(s) 115
MroNI GCCGGC 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 399
MscI TGGCCA 1 cut(s) 368
MseI TTAA 2 cut(s) 20, 72
MslI CAYNNNNRTG 1 cut(s) 483
Msp20I TGGCCA 1 cut(s) 368
MspI CCGG 4 cut(s) 102, 116, 418, 438
MspR9I CCNGG 3 cut(s) 413, 418, 439
MvaI CCWGG 1 cut(s) 413
MvnI CGCG 5 cut(s) 210, 212, 282, 284, 534
MwoI GCNNNNNNNGC 2 cut(s) 209, 255
NaeI GCCGGC 1 cut(s) 117
NciI CCSGG 2 cut(s) 418, 439
NdeII GATC 2 cut(s) 120, 460
NgoMIV GCCGGC 1 cut(s) 115
NlaIII CATG 1 cut(s) 378
NlaIV GGNNCC 3 cut(s) 360, 361, 410
NmeAIII GCCGAG 1 cut(s) 355
NspV TTCGAA 1 cut(s) 393
PauI GCGCGC 2 cut(s) 210, 280
PdiI GCCGGC 1 cut(s) 117
PdmI GAANNNNTTC 1 cut(s) 399
PfeI GAWTC 3 cut(s) 156, 444, 515
PkrI GCNGC 3 cut(s) 205, 260, 476
PleI GAGTC 2 cut(s) 391, 433
PpsI GAGTC 2 cut(s) 391, 433
PpuMI RGGWCCY 1 cut(s) 409
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 411
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 3 cut(s) 360, 361, 410
PspOMI GGGCCC 1 cut(s) 358
PspPI GGNCC 7 cut(s) 190, 358, 359, 409, 415, 420, 521
PspPPI RGGWCCY 1 cut(s) 409
PsuI RGATCY 1 cut(s) 460
PteI GCGCGC 2 cut(s) 210, 280
RsaI GTAC 3 cut(s) 45, 290, 341
RsaNI GTAC 3 cut(s) 44, 289, 340
RseI CAYNNNNRTG 1 cut(s) 483
Rsr2I CGGWCCG 1 cut(s) 420
RsrII CGGWCCG 1 cut(s) 420
SaqAI TTAA 2 cut(s) 20, 72
SatI GCNGC 3 cut(s) 204, 259, 475
Sau3AI GATC 2 cut(s) 120, 460
Sau96I GGNCC 7 cut(s) 190, 358, 359, 409, 415, 420, 521
SchI GAGTC 2 cut(s) 392, 434
ScrFI CCNGG 3 cut(s) 413, 418, 439
SduI GDGCHC 1 cut(s) 362
SfaNI GCATC 1 cut(s) 475
SfuI TTCGAA 1 cut(s) 393
SgrAI CRCCGGYG 1 cut(s) 115
SinI GGWCC 2 cut(s) 409, 420
SmiMI CAYNNNNRTG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
SsiI CCGC 3 cut(s) 193, 242, 268
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 3 cut(s) 411, 416, 437
StyI CCWWGG 1 cut(s) 363
TaiI ACGT 4 cut(s) 225, 240, 290, 456
TaqI TCGA 2 cut(s) 142, 393
TfiI GAWTC 3 cut(s) 156, 444, 515
Tru1I TTAA 2 cut(s) 20, 72
Tru9I TTAA 2 cut(s) 20, 72
TseI GCWGC 3 cut(s) 203, 258, 474
TspGWI ACGGA 1 cut(s) 149
VpaK11BI GGWCC 2 cut(s) 409, 420
XmnI GAANNNNTTC 1 cut(s) 399
XspI CTAG 1 cut(s) 320
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.