pycom05g04450

Indole-3-acetic acid-induced protein ARG7-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0003608
Physical Location & Seq
Reverse (-)
5228946 .. 5229398
453 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g04450.1

Sequence Viewer

Length: 453 bp
ATGTCGCCGGCGATCAGCAAATGCAACAAACTCCGACGAATTGTCAGAATCCGTCAAATGCTACAGCGCTGGCACAAGAAGGCGCGCCTCACGGCCGCACCCGCGCCGTCCGACGTCCCCTCGGGACACGTGGCGATCTGCGTAGGCAGCAGCTGCAAGCGGTTTGTCGTGCGCGCGACGTACCTGAACCACCCCATCTTCCAGAAGCTTCTAGTACAGGCCGAAGAGGAGTACGGCTTCGCCAACCAAGGCCCCTTGGCCATCCCGTGCGACGAGTCGTTTTTCGAAGAGGTTCTTCGGGTCCTGGCCCGGTCCGAGTCTGGTAACTCCGGCCGGTTCTTGAGCATCGAGGATCTCCAGAGACGCTGCCACGTTGATGCTCTGAATCAGCTCGAGTATTTGAGCGAATCTCGGCCGTTGCTTCGAGGGTTGGCTGATAAATCTGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Auxin_inducible

Protein Analysis

151

Amino Acids

16.91

Weight (kDa)

9.35

Isoelectric Point (pI)

73.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 16 - 102 2e-29 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 117
AccII CGCG 4 cut(s) 85, 104, 174, 176
AciI CCGC 3 cut(s) 96, 102, 160
AclWI GGATC 1 cut(s) 360
AcoI YGGCCR 4 cut(s) 93, 258, 331, 413
AcvI CACGTG 1 cut(s) 130
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 3 cut(s) 182, 216, 233
AfeI AGCGCT 1 cut(s) 68
AflIII ACRYGT 1 cut(s) 127
AhdI GACNNNNNGTC 1 cut(s) 41
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 3 cut(s) 153, 208, 391
AluI AGCT 3 cut(s) 153, 208, 391
Alw26I GTCTC 1 cut(s) 355
AlwI GGATC 1 cut(s) 360
AlwNI CAGNNNCTG 1 cut(s) 153
Ama87I CYCGRG 2 cut(s) 121, 392
Aor51HI AGCGCT 1 cut(s) 68
AoxI GGCC 7 cut(s) 93, 219, 250, 258, 306, 331, 413
ApeKI GCWGC 4 cut(s) 147, 150, 153, 366
AscI GGCGCGCC 1 cut(s) 83
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 6 cut(s) 69, 85, 87, 106, 174, 176
AspS9I GGNCC 4 cut(s) 251, 301, 307, 312
AsuC2I CCSGG 1 cut(s) 310
AsuII TTCGAA 1 cut(s) 285
AvaI CYCGRG 2 cut(s) 121, 392
AvaII GGWCC 2 cut(s) 301, 312
BalI TGGCCA 1 cut(s) 260
BbrPI CACGTG 1 cut(s) 130
BbvI GCAGC 4 cut(s) 140, 159, 162, 353
BccI CCATC 2 cut(s) 203, 269
BceAI ACGGC 4 cut(s) 91, 108, 250, 400
BciT130I CCWGG 1 cut(s) 305
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 355
BfaI CTAG 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 70
BisI GCNGC 5 cut(s) 96, 148, 151, 154, 367
BlsI GCNGC 5 cut(s) 97, 149, 152, 155, 368
Bme1390I CCNGG 2 cut(s) 305, 310
Bme18I GGWCC 2 cut(s) 301, 312
BmeRI GACNNNNNGTC 1 cut(s) 41
BmeT110I CYCGRG 2 cut(s) 121, 392
BmgT120I GGNCC 4 cut(s) 251, 301, 307, 312
BmiI GGNNCC 2 cut(s) 253, 302
BmrFI CCNGG 2 cut(s) 305, 310
BmsI GCATC 2 cut(s) 354, 367
BplI GAGNNNNNCTC 2 cut(s) 394, 426
BpmI CTGGAG 1 cut(s) 341
Bpu14I TTCGAA 1 cut(s) 285
BpuEI CTTGAG 1 cut(s) 361
BpuMI CCSGG 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 130
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 3 cut(s) 120, 247, 255
Bse118I RCCGGY 2 cut(s) 7, 333
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 3 cut(s) 120, 247, 255
BseGI GGATG 1 cut(s) 261
BsePI GCGCGC 2 cut(s) 83, 172
BseRI GAGGAG 1 cut(s) 242
BseX3I CGGCCG 3 cut(s) 93, 331, 413
BseXI GCAGC 4 cut(s) 140, 159, 162, 353
Bsh1236I CGCG 4 cut(s) 85, 104, 174, 176
Bsh1285I CGRYCG 3 cut(s) 96, 334, 416
BshFI GGCC 7 cut(s) 95, 221, 252, 260, 308, 333, 415
BsiEI CGRYCG 3 cut(s) 96, 334, 416
BsiHKCI CYCGRG 2 cut(s) 121, 392
BsiSI CCGG 4 cut(s) 8, 310, 330, 334
BslFI GGGAC 2 cut(s) 101, 138
BsmAI GTCTC 1 cut(s) 355
BsmBI CGTCTC 1 cut(s) 355
BsmFI GGGAC 2 cut(s) 101, 138
BsnI GGCC 7 cut(s) 95, 221, 252, 260, 308, 333, 415
BsoBI CYCGRG 2 cut(s) 121, 392
Bsp119I TTCGAA 1 cut(s) 285
Bsp143I GATC 3 cut(s) 12, 135, 352
BspACI CCGC 3 cut(s) 96, 102, 160
BspANI GGCC 7 cut(s) 95, 221, 252, 260, 308, 333, 415
BspFNI CGCG 4 cut(s) 85, 104, 174, 176
BspLI GGNNCC 2 cut(s) 253, 302
BspPI GGATC 1 cut(s) 360
BspT104I TTCGAA 1 cut(s) 285
BsrFI RCCGGY 2 cut(s) 7, 333
BssAI RCCGGY 2 cut(s) 7, 333
BssECI CCNNGG 3 cut(s) 120, 247, 255
BssHII GCGCGC 2 cut(s) 83, 172
BssMI GATC 3 cut(s) 12, 135, 352
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 247, 255
Bst2UI CCWGG 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 219, 282
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 153
BstBAI YACGTR 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 285
BstC8I GCNNGC 5 cut(s) 9, 71, 85, 158, 174
BstF5I GGATG 1 cut(s) 261
BstFNI CGCG 4 cut(s) 85, 104, 174, 176
BstH2I RGCGCY 1 cut(s) 70
BstHHI GCGC 6 cut(s) 69, 85, 87, 106, 174, 176
BstKTI GATC 3 cut(s) 15, 138, 355
BstMAI GTCTC 1 cut(s) 355
BstMBI GATC 3 cut(s) 12, 135, 352
BstMCI CGRYCG 3 cut(s) 96, 334, 416
BstMWI GCNNNNNNNGC 3 cut(s) 101, 147, 153
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 2 cut(s) 303, 308
BstSFI CTRYAG 1 cut(s) 62
BstUI CGCG 4 cut(s) 85, 104, 174, 176
BstV1I GCAGC 4 cut(s) 140, 159, 162, 353
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BstZI CGGCCG 3 cut(s) 93, 331, 413
BsuRI GGCC 7 cut(s) 95, 221, 252, 260, 308, 333, 415
BtsCI GGATG 1 cut(s) 261
Cac8I GCNNGC 5 cut(s) 9, 71, 85, 158, 174
CaiI CAGNNNCTG 1 cut(s) 153
CfoI GCGC 6 cut(s) 69, 85, 87, 106, 174, 176
Cfr10I RCCGGY 2 cut(s) 7, 333
Cfr13I GGNCC 4 cut(s) 251, 301, 307, 312
CpoI CGGWCCG 1 cut(s) 312
CseI GACGC 1 cut(s) 372
Csp6I GTAC 3 cut(s) 181, 215, 232
CspI CGGWCCG 1 cut(s) 312
CviQI GTAC 3 cut(s) 181, 215, 232
DpnI GATC 3 cut(s) 14, 137, 354
DpnII GATC 3 cut(s) 12, 135, 352
DriI GACNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 4 cut(s) 93, 258, 331, 413
EagI CGGCCG 3 cut(s) 93, 331, 413
Eam1104I CTCTTC 2 cut(s) 219, 282
Eam1105I GACNNNNNGTC 1 cut(s) 41
EarI CTCTTC 2 cut(s) 219, 282
EclXI CGGCCG 3 cut(s) 93, 331, 413
Eco130I CCWWGG 2 cut(s) 247, 255
Eco47I GGWCC 2 cut(s) 301, 312
Eco47III AGCGCT 1 cut(s) 68
Eco52I CGGCCG 3 cut(s) 93, 331, 413
Eco72I CACGTG 1 cut(s) 130
Eco88I CYCGRG 2 cut(s) 121, 392
EcoO109I RGGNCCY 2 cut(s) 251, 301
EcoRII CCWGG 1 cut(s) 303
EcoT14I CCWWGG 2 cut(s) 247, 255
ErhI CCWWGG 2 cut(s) 247, 255
Esp3I CGTCTC 1 cut(s) 355
FaiI YATR 1 cut(s) 448
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 2 cut(s) 101, 138
FauI CCCGC 1 cut(s) 109
Fnu4HI GCNGC 5 cut(s) 96, 148, 151, 154, 367
FokI GGATG 1 cut(s) 248
Fsp4HI GCNGC 5 cut(s) 96, 148, 151, 154, 367
FspBI CTAG 1 cut(s) 212
GlaI GCGC 6 cut(s) 68, 84, 86, 105, 173, 175
GluI GCNGC 5 cut(s) 96, 148, 151, 154, 367
GsuI CTGGAG 1 cut(s) 341
HaeII RGCGCY 1 cut(s) 70
HaeIII GGCC 7 cut(s) 95, 221, 252, 260, 308, 333, 415
HapII CCGG 4 cut(s) 8, 310, 330, 334
HgaI GACGC 1 cut(s) 372
HhaI GCGC 6 cut(s) 69, 85, 87, 106, 174, 176
Hin1I GRCGYC 1 cut(s) 114
Hin6I GCGC 6 cut(s) 67, 83, 85, 104, 172, 174
HinP1I GCGC 6 cut(s) 67, 83, 85, 104, 172, 174
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 5 cut(s) 48, 275, 317, 385, 407
HpaII CCGG 4 cut(s) 8, 310, 330, 334
Hpy188I TCNGA 5 cut(s) 35, 47, 112, 316, 384
Hpy188III TCNNGA 4 cut(s) 123, 202, 340, 358
Hpy99I CGWCG 4 cut(s) 39, 116, 181, 275
HpyAV CCTTC 1 cut(s) 73
HpyCH4IV ACGT 4 cut(s) 114, 129, 179, 372
HpyCH4V TGCA 2 cut(s) 24, 156
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 147, 153
HpySE526I ACGT 4 cut(s) 114, 129, 179, 372
Hsp92I GRCGYC 1 cut(s) 114
HspAI GCGC 6 cut(s) 67, 83, 85, 104, 172, 174
KroI GCCGGC 1 cut(s) 7
KroNI GCCGGC 1 cut(s) 9
Kzo9I GATC 3 cut(s) 12, 135, 352
Lsp1109I GCAGC 4 cut(s) 140, 159, 162, 353
LweI GCATC 2 cut(s) 354, 367
MaeI CTAG 1 cut(s) 212
MaeII ACGT 4 cut(s) 114, 129, 179, 372
MaeIII GTNAC 1 cut(s) 323
MalI GATC 3 cut(s) 14, 137, 354
MboI GATC 3 cut(s) 12, 135, 352
MboII GAAGA 4 cut(s) 190, 236, 287, 299
MflI RGATCY 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 1 cut(s) 39
MluNI TGGCCA 1 cut(s) 260
MlyI GAGTC 2 cut(s) 284, 326
MmeI TCCRAC 2 cut(s) 58, 135
MnlI CCTC 6 cut(s) 98, 130, 220, 283, 343, 419
Mox20I TGGCCA 1 cut(s) 260
MreI CGCCGGCG 1 cut(s) 7
MroNI GCCGGC 1 cut(s) 7
MroXI GAANNNNTTC 1 cut(s) 291
MscI TGGCCA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 375
Msp20I TGGCCA 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 153
MspI CCGG 4 cut(s) 8, 310, 330, 334
MspR9I CCNGG 2 cut(s) 305, 310
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 4 cut(s) 85, 104, 174, 176
MwoI GCNNNNNNNGC 3 cut(s) 101, 147, 153
NaeI GCCGGC 1 cut(s) 9
NciI CCSGG 1 cut(s) 310
NdeII GATC 3 cut(s) 12, 135, 352
NgoMIV GCCGGC 1 cut(s) 7
NlaIV GGNNCC 2 cut(s) 253, 302
NmeAIII GCCGAG 1 cut(s) 391
NspV TTCGAA 1 cut(s) 285
PaeR7I CTCGAG 1 cut(s) 392
PalAI GGCGCGCC 1 cut(s) 83
PauI GCGCGC 2 cut(s) 83, 172
PdiI GCCGGC 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 48, 385, 407
PkrI GCNGC 5 cut(s) 97, 149, 152, 155, 368
PleI GAGTC 2 cut(s) 283, 325
PmaCI CACGTG 1 cut(s) 130
PmlI CACGTG 1 cut(s) 130
PpsI GAGTC 2 cut(s) 283, 325
Ppu21I YACGTR 1 cut(s) 130
PpuMI RGGWCCY 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 303
PspCI CACGTG 1 cut(s) 130
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 253, 302
PspPI GGNCC 4 cut(s) 251, 301, 307, 312
PspPPI RGGWCCY 1 cut(s) 301
PspXI VCTCGAGB 1 cut(s) 392
PstNI CAGNNNCTG 1 cut(s) 153
PsuI RGATCY 1 cut(s) 352
PteI GCGCGC 2 cut(s) 83, 172
PvuII CAGCTG 1 cut(s) 153
RsaI GTAC 3 cut(s) 182, 216, 233
RsaNI GTAC 3 cut(s) 181, 215, 232
RseI CAYNNNNRTG 1 cut(s) 375
Rsr2I CGGWCCG 1 cut(s) 312
RsrII CGGWCCG 1 cut(s) 312
SatI GCNGC 5 cut(s) 96, 148, 151, 154, 367
Sau3AI GATC 3 cut(s) 12, 135, 352
Sau96I GGNCC 4 cut(s) 251, 301, 307, 312
SchI GAGTC 2 cut(s) 284, 326
ScrFI CCNGG 2 cut(s) 305, 310
SetI ASST 9 cut(s) 117, 132, 155, 182, 186, 210, 294, 375, 393
SfaNI GCATC 2 cut(s) 354, 367
SfcI CTRYAG 1 cut(s) 62
Sfr274I CTCGAG 1 cut(s) 392
SfuI TTCGAA 1 cut(s) 285
SgrAI CRCCGGYG 1 cut(s) 7
SgsI GGCGCGCC 1 cut(s) 83
SinI GGWCC 2 cut(s) 301, 312
SlaI CTCGAG 1 cut(s) 392
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 2 cut(s) 340, 392
SmoI CTYRAG 2 cut(s) 340, 392
Sse9I AATT 1 cut(s) 39
SsiI CCGC 3 cut(s) 96, 102, 160
SspMI CTAG 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 303, 308
StyI CCWWGG 2 cut(s) 247, 255
TaiI ACGT 4 cut(s) 117, 132, 182, 375
TaqI TCGA 4 cut(s) 285, 348, 393, 424
TasI AATT 1 cut(s) 39
TatI WGTACW 1 cut(s) 214
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 3 cut(s) 48, 385, 407
TseI GCWGC 4 cut(s) 147, 150, 153, 366
TspGWI ACGGA 1 cut(s) 41
VpaK11BI GGWCC 2 cut(s) 301, 312
XhoI CTCGAG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 1 cut(s) 212
ZraI GACGTC 1 cut(s) 115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.