Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002707

Physical Location & Seq

Genomic Coordinates Forward (+)

7245919 .. 7248355

Gene Structure 2437 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1087400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 753 bp

ATGTCCAGGGCTCGCGTATACGCGGACGTCAACGTTCAGCGCCCCAGAGATTACTGGGATTACGAATCCGTCACTCTTCAATGGGGTGAACAAGATGACTATGAGGTTGTTAGGAAGATCGGAAGAGGAAAATACAGTGAGGTCTTTGAAGGTGTAAATGTCACCAAAAACGAAAAGTGCGTTATGAAGATCCTTAAACCCGTCATGAAAAAGAAGATAAAAAGGGAGATGAAGATACTACAGAATCTTTGTGGTGGTACAAACATCGTGAAGCTGCTTGACATTGTCAGGGATGAACACTCAAAAACTCCTAGCTTGATCTTTGAGTATGTGAACAGTGCAGATTTCAAAGTGTTGTATCCCACACTGACGGATTATGACGTTCGCTACTATATATATGAACTTCTCAAGGCATTGGATTACTGCCATTCGCAGGGCATAATGCATCGAGATGTCAAGCCTCACAATGTTATGATTGATCATGATCTCCGGAAACTTCGCTTGATTGATTGGGGTCTCGCTGATTTCTACCATCCTGGAAAAGAGTACAATGTCCGAGTAGCTTCAAGGCACTATAAGGGGCCTGAACTTCTCGTGGATCTGCAAGACTATGACTATTCGTTAGACTTGTGGAGCCTTGGCTGTATGTTTGCAGGAATGATCTTTCGCAAGGAACAATTCTTCTATGGTCATGACAATCAGGACCAGCTAGTGAAAATTGCTACGGTACTTTTGGTTTCGTACTTGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0008150 GO:0031974 GO:0031981 GO:0042752 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0050789 GO:0065007 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko03008 ko04064 ko04137 ko04139 ko04310 ko04520 ko04712 ko05162 ko05168 ko05169 map03008 map04064 map04137 map04139 map04310 map04520 map04712 map05162 map05168 map05169

Pfam Domains

Protein Families

Pkinase

Protein Analysis

251

Amino Acids

29.54

Weight (kDa)

7.66

Isoelectric Point (pI)

32.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	34 - 239	1.8e-51	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	36 - 237	3.4e-19	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002707)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g33300 FvH4_2g33300 FvH4_2g33300 FvH4_2g33300 FvH4_2g33300
malus_domestica	MD08G1087400.v1.1 MD15G1072400.v1.1 MD15G1314700.v1.1
prunus_persica	Prupe.1G425000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0357221 RchiOBHm_Chr1g0362391 RchiOBHm_Chr2g0131551 RchiOBHm_Chr6g0309321
rosa_laevigata	RLG00000009840 RLG00000010574 RLG00000021364 RLG00000031506
rosa_multiflora	Rmu_sc0003576.1_g000007 Rmu_sc0015692.1_g000002
rosa_roxburghii	Rroxscaffold_3G00228160 Rroxscaffold_6G00412270 Rroxscaffold_7G00159490
rosa_rugosa	Rorug01G0255000 Rorug05G0444500 Rorug05G0444500 Rorug06G0371000
rosa_samantha	Rh2BG357900 Rh5AG062100 Rh5AG134700 Rh5BG310400 Rh5DG320900 Rh6AG235600 Rh6AG484400 Rh6BG493500 Rh6CG498300 Rh6DG484800
rosa_wichuraiana	Rw6G028820 Rw6G042170 Rw7G020100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	30
AccI	GTMKAC	1 cut(s)	18
AccII	CGCG	2 cut(s)	15, 23
AccIII	TCCGGA	1 cut(s)	489
AciI	CCGC	1 cut(s)	23
AclI	AACGTT	1 cut(s)	33
AclWI	GGATC	2 cut(s)	184, 606
AcyI	GRCGYC	1 cut(s)	27
AfaI	GTAC	4 cut(s)	259, 548, 729, 743
AfiI	CCNNNNNNNGG	1 cut(s)	433
AgsI	TTSAA	4 cut(s)	80, 149, 349, 567
AjnI	CCWGG	2 cut(s)	5, 535
AluBI	AGCT	4 cut(s)	274, 315, 563, 709
AluI	AGCT	4 cut(s)	274, 315, 563, 709
Alw26I	GTCTC	1 cut(s)	521
AlwI	GGATC	2 cut(s)	184, 606
Aor13HI	TCCGGA	1 cut(s)	489
AoxI	GGCC	1 cut(s)	581
ApeKI	GCWGC	1 cut(s)	274
AspLEI	GCGC	1 cut(s)	42
AspS9I	GGNCC	2 cut(s)	581, 703
AsuHPI	GGTGA	2 cut(s)	98, 154
AvaII	GGWCC	1 cut(s)	703
BanII	GRGCYC	1 cut(s)	13
BauI	CACGAG	1 cut(s)	593
BbvI	GCAGC	1 cut(s)	261
BccI	CCATC	1 cut(s)	540
BciT130I	CCWGG	2 cut(s)	7, 537
BciVI	GTATCC	1 cut(s)	369
BclI	TGATCA	1 cut(s)	478
BcoDI	GTCTC	1 cut(s)	521
BfaI	CTAG	2 cut(s)	312, 710
BfmI	CTRYAG	1 cut(s)	239
BfoI	RGCGCY	1 cut(s)	43
BfuI	GTATCC	1 cut(s)	369
BisI	GCNGC	1 cut(s)	275
BlsI	GCNGC	1 cut(s)	276
Bme1390I	CCNGG	2 cut(s)	7, 537
Bme18I	GGWCC	1 cut(s)	703
BmgT120I	GGNCC	2 cut(s)	581, 703
BmiI	GGNNCC	2 cut(s)	582, 635
BmrFI	CCNGG	2 cut(s)	7, 537
BmrI	ACTGGG	1 cut(s)	64
BmsI	GCATC	1 cut(s)	454
BmuI	ACTGGG	1 cut(s)	64
BpuEI	CTTGAG	1 cut(s)	392
BsaBI	GATNNNNATC	1 cut(s)	483
BsaHI	GRCGYC	1 cut(s)	27
BsaI	GGTCTC	1 cut(s)	521
BsaJI	CCNNGG	2 cut(s)	6, 637
BsaWI	WCCGGW	1 cut(s)	489
Bsc4I	CCNNNNNNNGG	1 cut(s)	433
Bse1I	ACTGG	1 cut(s)	59
Bse8I	GATNNNNATC	1 cut(s)	483
BseAI	TCCGGA	1 cut(s)	489
BseBI	CCWGG	2 cut(s)	7, 537
BseDI	CCNNGG	2 cut(s)	6, 637
BseGI	GGATG	2 cut(s)	298, 532
BseJI	GATNNNNATC	1 cut(s)	483
BseLI	CCNNNNNNNGG	1 cut(s)	433
BseNI	ACTGG	1 cut(s)	59
BseXI	GCAGC	1 cut(s)	261
BsgI	GTGCAG	1 cut(s)	360
Bsh1236I	CGCG	2 cut(s)	15, 23
BshFI	GGCC	1 cut(s)	583
BsiSI	CCGG	1 cut(s)	490
BslI	CCNNNNNNNGG	1 cut(s)	433
BsmAI	GTCTC	1 cut(s)	521
BsnI	GGCC	1 cut(s)	583
Bso31I	GGTCTC	1 cut(s)	521
Bsp1286I	GDGCHC	1 cut(s)	13
Bsp13I	TCCGGA	1 cut(s)	489
Bsp143I	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
BspACI	CCGC	1 cut(s)	23
BspANI	GGCC	1 cut(s)	583
BspEI	TCCGGA	1 cut(s)	489
BspFNI	CGCG	2 cut(s)	15, 23
BspHI	TCATGA	3 cut(s)	204, 481, 691
BspLI	GGNNCC	2 cut(s)	582, 635
BspPI	GGATC	2 cut(s)	184, 606
BspTNI	GGTCTC	1 cut(s)	521
BsrI	ACTGG	1 cut(s)	59
BssECI	CCNNGG	2 cut(s)	6, 637
BssMI	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
BssNAI	GTATAC	1 cut(s)	19
BssNI	GRCGYC	1 cut(s)	27
BssSI	CACGAG	1 cut(s)	593
BssT1I	CCWWGG	1 cut(s)	637
Bst1107I	GTATAC	1 cut(s)	19
Bst2BI	CACGAG	1 cut(s)	593
Bst2UI	CCWGG	2 cut(s)	7, 537
Bst4CI	ACNGT	3 cut(s)	137, 338, 727
Bst6I	CTCTTC	2 cut(s)	81, 118
BstACI	GRCGYC	1 cut(s)	27
BstC8I	GCNNGC	1 cut(s)	13
BstF5I	GGATG	2 cut(s)	298, 532
BstFNI	CGCG	2 cut(s)	15, 23
BstH2I	RGCGCY	1 cut(s)	43
BstHHI	GCGC	1 cut(s)	42
BstKTI	GATC	7 cut(s)	120, 192, 321, 481, 487, 601, 663
BstMAI	GTCTC	1 cut(s)	521
BstMBI	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
BstNI	CCWGG	2 cut(s)	7, 537
BstSCI	CCNGG	2 cut(s)	5, 535
BstSFI	CTRYAG	1 cut(s)	239
BstUI	CGCG	2 cut(s)	15, 23
BstV1I	GCAGC	1 cut(s)	261
BstX2I	RGATCY	2 cut(s)	189, 598
BstYI	RGATCY	2 cut(s)	189, 598
BstZ17I	GTATAC	1 cut(s)	19
BsuI	GTATCC	1 cut(s)	369
BsuRI	GGCC	1 cut(s)	583
BtsCI	GGATG	2 cut(s)	298, 532
BtsIMutI	CAGTG	3 cut(s)	142, 343, 365
Cac8I	GCNNGC	1 cut(s)	13
CciI	TCATGA	3 cut(s)	204, 481, 691
CfoI	GCGC	1 cut(s)	42
Cfr13I	GGNCC	2 cut(s)	581, 703
Csp6I	GTAC	4 cut(s)	258, 547, 728, 742
CviAII	CATG	3 cut(s)	205, 482, 692
CviQI	GTAC	4 cut(s)	258, 547, 728, 742
DpnI	GATC	7 cut(s)	119, 191, 320, 480, 486, 600, 662
DpnII	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
Eam1104I	CTCTTC	2 cut(s)	81, 118
EarI	CTCTTC	2 cut(s)	81, 118
Eco130I	CCWWGG	1 cut(s)	637
Eco24I	GRGCYC	1 cut(s)	13
Eco31I	GGTCTC	1 cut(s)	521
Eco47I	GGWCC	1 cut(s)	703
EcoO109I	RGGNCCY	1 cut(s)	581
EcoRII	CCWGG	2 cut(s)	5, 535
EcoT14I	CCWWGG	1 cut(s)	637
EcoT22I	ATGCAT	1 cut(s)	447
EcoT38I	GRGCYC	1 cut(s)	13
ErhI	CCWWGG	1 cut(s)	637
FaeI	CATG	3 cut(s)	208, 485, 695
FatI	CATG	3 cut(s)	204, 481, 691
FbaI	TGATCA	1 cut(s)	478
FblI	GTMKAC	1 cut(s)	18
Fnu4HI	GCNGC	1 cut(s)	275
FokI	GGATG	2 cut(s)	305, 519
FriOI	GRGCYC	1 cut(s)	13
Fsp4HI	GCNGC	1 cut(s)	275
FspBI	CTAG	2 cut(s)	312, 710
GlaI	GCGC	1 cut(s)	41
GluI	GCNGC	1 cut(s)	275
HaeII	RGCGCY	1 cut(s)	43
HaeIII	GGCC	1 cut(s)	583
HapII	CCGG	1 cut(s)	490
HhaI	GCGC	1 cut(s)	42
Hin1I	GRCGYC	1 cut(s)	27
Hin1II	CATG	3 cut(s)	208, 485, 695
Hin6I	GCGC	1 cut(s)	40
HinP1I	GCGC	1 cut(s)	40
HincII	GTYRAC	1 cut(s)	31
HindII	GTYRAC	1 cut(s)	31
HinfI	GANTC	2 cut(s)	65, 244
HpaII	CCGG	1 cut(s)	490
HphI	GGTGA	2 cut(s)	98, 154
Hpy166II	GTNNAC	4 cut(s)	19, 31, 89, 334
Hpy188I	TCNGA	2 cut(s)	122, 557
Hpy188III	TCNNGA	7 cut(s)	205, 268, 449, 482, 490, 692, 701
Hpy8I	GTNNAC	4 cut(s)	19, 31, 89, 334
HpyAV	CCTTC	1 cut(s)	143
HpyCH4III	ACNGT	3 cut(s)	137, 338, 727
HpyCH4IV	ACGT	3 cut(s)	27, 33, 381
HpyCH4V	TGCA	4 cut(s)	341, 445, 604, 653
HpySE526I	ACGT	3 cut(s)	27, 33, 381
Hsp92I	GRCGYC	1 cut(s)	27
Hsp92II	CATG	3 cut(s)	208, 485, 695
HspAI	GCGC	1 cut(s)	40
Kpn2I	TCCGGA	1 cut(s)	489
Ksp22I	TGATCA	1 cut(s)	478
Kzo9I	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
LmnI	GCTCC	1 cut(s)	633
Lsp1109I	GCAGC	1 cut(s)	261
LweI	GCATC	1 cut(s)	454
MaeI	CTAG	2 cut(s)	312, 710
MaeII	ACGT	3 cut(s)	27, 33, 381
MaeIII	GTNAC	2 cut(s)	70, 160
MalI	GATC	7 cut(s)	119, 191, 320, 480, 486, 600, 662
MboI	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
MboII	GAAGA	7 cut(s)	68, 127, 135, 199, 226, 244, 673
MflI	RGATCY	2 cut(s)	189, 598
MhlI	GDGCHC	1 cut(s)	13
MluCI	AATT	2 cut(s)	677, 717
MnlI	CCTC	4 cut(s)	97, 119, 133, 471
Mph1103I	ATGCAT	1 cut(s)	447
MroI	TCCGGA	1 cut(s)	489
MseI	TTAA	1 cut(s)	195
MslI	CAYNNNNRTG	1 cut(s)	450
MspI	CCGG	1 cut(s)	490
MspR9I	CCNGG	2 cut(s)	7, 537
MvaI	CCWGG	2 cut(s)	7, 537
MvnI	CGCG	2 cut(s)	15, 23
NdeII	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
NlaIII	CATG	3 cut(s)	208, 485, 695
NlaIV	GGNNCC	2 cut(s)	582, 635
NmuCI	GTSAC	2 cut(s)	70, 160
NsiI	ATGCAT	1 cut(s)	447
PagI	TCATGA	3 cut(s)	204, 481, 691
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PfeI	GAWTC	2 cut(s)	65, 244
PflFI	GACNNNGTC	1 cut(s)	284
PfoI	TCCNGGA	1 cut(s)	535
PkrI	GCNGC	1 cut(s)	276
Psp1406I	AACGTT	1 cut(s)	33
Psp6I	CCWGG	2 cut(s)	5, 535
PspGI	CCWGG	2 cut(s)	5, 535
PspN4I	GGNNCC	2 cut(s)	582, 635
PspPI	GGNCC	2 cut(s)	581, 703
PsuI	RGATCY	2 cut(s)	189, 598
PsyI	GACNNNGTC	1 cut(s)	284
RsaI	GTAC	4 cut(s)	259, 548, 729, 743
RsaNI	GTAC	4 cut(s)	258, 547, 728, 742
RseI	CAYNNNNRTG	1 cut(s)	450
SaqAI	TTAA	1 cut(s)	195
SatI	GCNGC	1 cut(s)	275
Sau3AI	GATC	7 cut(s)	117, 189, 318, 478, 484, 598, 660
Sau96I	GGNCC	2 cut(s)	581, 703
ScrFI	CCNGG	2 cut(s)	7, 537
SduI	GDGCHC	1 cut(s)	13
SfaNI	GCATC	1 cut(s)	454
SfcI	CTRYAG	1 cut(s)	239
SinI	GGWCC	1 cut(s)	703
SmiMI	CAYNNNNRTG	1 cut(s)	450
SmlI	CTYRAG	1 cut(s)	407
SmoI	CTYRAG	1 cut(s)	407
Sse9I	AATT	2 cut(s)	677, 717
SsiI	CCGC	1 cut(s)	23
SspMI	CTAG	2 cut(s)	312, 710
StyD4I	CCNGG	2 cut(s)	5, 535
StyI	CCWWGG	1 cut(s)	637
TaaI	ACNGT	3 cut(s)	137, 338, 727
TaiI	ACGT	3 cut(s)	30, 36, 384
TaqI	TCGA	1 cut(s)	448
TasI	AATT	2 cut(s)	677, 717
TatI	WGTACW	1 cut(s)	546
TfiI	GAWTC	2 cut(s)	65, 244
Tru1I	TTAA	1 cut(s)	195
Tru9I	TTAA	1 cut(s)	195
TscAI	CASTG	3 cut(s)	142, 343, 372
TseFI	GTSAC	2 cut(s)	70, 160
TseI	GCWGC	1 cut(s)	274
Tsp45I	GTSAC	2 cut(s)	70, 160
TspDTI	ATGAA	5 cut(s)	200, 221, 245, 309, 414
TspGWI	ACGGA	2 cut(s)	58, 386
TspRI	CASTG	3 cut(s)	142, 343, 372
Tth111I	GACNNNGTC	1 cut(s)	284
VpaK11BI	GGWCC	1 cut(s)	703
XmiI	GTMKAC	1 cut(s)	18
XspI	CTAG	2 cut(s)	312, 710
ZraI	GACGTC	1 cut(s)	28
Zsp2I	ATGCAT	1 cut(s)	447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.