Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0000139

Physical Location & Seq

Genomic Coordinates Forward (+)

61562040 .. 61569368

Gene Structure 7329 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0444500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1869 bp

ATGTCGGGATTACTTAATTCTTCTCTAAACGGATCAGCCTCAAATCTTCCAGATAGTAGTGGACGTTTTGCCACATCTTTCTCTGGTCAATCGGGTGCTGCCTCTCCTGTATTTCATCACGCAGGATCTATTCAAGGATTGCATAACCTTCATGGGAGCTTCAATGTTCCTAACATGCCTGGTACATTAACTTCAAGAAACTCAACATTAACTAATGTTCCTTCTGGTGGAGTCCAACAACCAACTGGAAGCCTTTCTGGTGGACGATTTTCATCAAATAATCTTCCTGTTGCTCTGTCTCAGTTATCTCATGGAAGCTCTCATGGACATTCAGGAGTCACTAATAGAGGAGGCCAAATTTTGGGAAATGCAGGTCCACGGATTACAAGTTCTATGGGAAACATGGTTGGTGGGGGCAACATTGGGAGGAGTATTAGTTCTGGTGGAGGCTTGTCGGTGCCTGGTCTTACTTCCCGTCTAAATTTAAGTGGAAATAGTGGATCTGGTAGCTTAACTGTCCAAGGGCAGAATCGCTTGATGGGTGGTGTGATTCCACAAGGGTCCCCGCAGGTTATTTCAATGTTGGGAAATTCTTATCCAAGTGCTGGAGGTCCACTTTCTCAAAGCCATGTCCAAGTGAATAATTTGAGCTCTATGGGAATGTTGAATGATGTAAATTCTAATGACAGTTCTCCTTTTGACATGAATGATTTCCCTCAATTAACAAGCCGGCCAAGTTCTGCGGGAGGCCCTCAAGGGCAATTGGGTTCATTGCGAAAACAAGGTCTTGGTGTTAGTCCTATTGTTCAACAAAACCAAGAGTTCAGCATCCAAAATGAAGATTTTCCGGCTTTGCCTGGATTTAAAGGTGGTAATTCTGATTATCCAATGGATATGCACCAGAAAGAACAACTTCATGACAATACCGTGTCAATGATGCAATCTCAGCACTTCTCAATGGGAAGATCTGCCGGATTTAACTTGGGAGGGACTTATTCATCTCATCGTCCACAGCAGCAACAACAACATGCTCCATCAGTCAGCAATAGTGGTGTCTCATTTTCTCAAGTAAACAATCAGGATCTTCTCCATTTGCATGGCTCAGATATGTTCCCATCATCGCATTCAACCTATCATTCACAGACAAGTGGACCTCCTGGCATTGGATTGAGACCTTTAAATTCGGCCAATACAGTTTCTGGCATGGGTTCTTATGACCAGCTTATCCAGCAGTATCAACAACACCAAAATCAGTCACAGTTTCGTCTGCAACAGATGTCACCGGTCAATCAATCGTTTAGGGATCAGGGAATTAAGTCGATGCAGACCTCGCAATCTGCTCCTGACCCATTTGGTTTGCTTGGTTTGTTAAGTGTCATAAGGATGAGTGATCCTGATTTAACATCTCTTGCACTTGGAATTGATTTGACAACGCTTGGCTTAAACTTGAATTCAACAGAAAATCTGCACAAGACTTTTGGTTCCCCATGGTCTGACGAGCCAGCTAAGGGTGATCCAGAGTTTAGTGTTCCCCAATGTTATTATGCTAAACAACCACCTGCACTACATCAAGGATATTTTTCAAAGTTCTCGGTGGAAACATTATTTTACATATTTTACAGCATGCCTAAAGATGAAGCTCAATTACATGCTGCATATGAACTTTATAACAAGGGCTGGTTTTATCACAAGGAGCACCATCTATGGATTGCAAGAGTCCCCAATATGGAGCCACTTGTCAAGACAAACACATATGAGAGAGGGTCTTATCACTGTTTTGATCCCAACACATTTGAAATAGTTCGAAAGGAAAATTTTGTTGTGCATTATGATGTGCTGGATAAGAGACCGTCCTTACCGCAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005789 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009987 GO:0010467 GO:0012505 GO:0016020 GO:0016021 GO:0016485 GO:0016787 GO:0019538 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0042175 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0051604 GO:0070011 GO:0071586 GO:0071704 GO:0080120 GO:0098827 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00900 ko01130 map00900 map01130

Pfam Domains

Protein Families

Peptidase_M48 Peptidase_M48_N

Protein Analysis

622

Amino Acids

66.79

Weight (kDa)

6.83

Isoelectric Point (pI)

54.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NOT2_3_5_C	PF04153	490 - 613	1.9e-39	NOT2/NOT3/NOT5 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0000139)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g08100 FvH4_1g08100 FvH4_3g41771 FvH4_3g42301 FvH4_3g42302 FvH4_3g42303 FvH4_3g42304 FvH4_3g42321 FvH4_3g42360 FvH4_3g42420 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42460 FvH4_3g42470 FvH4_3g42490 FvH4_3g42581 FvH4_3g42582 FvH4_5g30990 FvH4_5g30990 FvH4_5g37874 FvH4_6g47401
prunus_persica	Prupe.2G278400_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.6G215000_v2.0.a1
pyrus_communis	pycom15g26480
rosa_chinensis	RchiOBHm_Chr1g0361131 RchiOBHm_Chr1g0380591 RchiOBHm_Chr1g0380601 RchiOBHm_Chr5g0075661 RchiOBHm_Chr5g0075691 RchiOBHm_Chr5g0075921 RchiOBHm_Chr5g0075931 RchiOBHm_Chr5g0075941 RchiOBHm_Chr5g0076031 RchiOBHm_Chr5g0076061 RchiOBHm_Chr5g0076091 RchiOBHm_Chr5g0076101 RchiOBHm_Chr5g0076131 RchiOBHm_Chr5g0076141 RchiOBHm_Chr5g0076151 RchiOBHm_Chr7g0226061 RchiOBHm_Chr7g0226071 RchiOBHm_Chr7g0226091 RchiOBHm_Chr7g0226431 RchiOBHm_Chr7g0226441
rosa_laevigata	RLG00000027157 RLG00000036604
rosa_multiflora	Rmu_co8028714.1_g000001 Rmu_co8069518.1_g000001 Rmu_co8225880.1_g000001 Rmu_co8266415.1_g000001 Rmu_sc0001470.1_g000003 Rmu_sc0001470.1_g000004 Rmu_sc0001764.1_g000007 Rmu_sc0002627.1_g000001 Rmu_sc0002652.1_g000008 Rmu_sc0002652.1_g000010 Rmu_sc0002652.1_g000011 Rmu_sc0002652.1_g000013 Rmu_sc0002652.1_g000016 Rmu_sc0002820.1_g000003 Rmu_sc0002820.1_g000004 Rmu_sc0002863.1_g000037 Rmu_sc0003016.1_g000001 Rmu_sc0003601.1_g000001 Rmu_sc0003945.1_g000010 Rmu_sc0004200.1_g000005 Rmu_sc0004250.1_g000018 Rmu_sc0004647.1_g000006 Rmu_sc0004647.1_g000007 Rmu_sc0005762.1_g000005 Rmu_sc0005762.1_g000011 Rmu_sc0005762.1_g000014 Rmu_sc0005961.1_g000010 Rmu_sc0007791.1_g000001 Rmu_sc0007791.1_g000006 Rmu_sc0007791.1_g000010 Rmu_sc0008955.1_g000006 Rmu_sc0008955.1_g000008 Rmu_sc0008955.1_g000019 Rmu_sc0008955.1_g000021 Rmu_sc0010684.1_g000002 Rmu_sc0012777.1_g000003 Rmu_sc0014532.1_g000001 Rmu_sc0018126.1_g000001 Rmu_sc0021483.1_g000001 Rmu_sc0025529.1_g000001 Rmu_sc0027085.1_g000001 Rmu_sc0027085.1_g000003 Rmu_sc0028007.1_g000001 Rmu_sc0031697.1_g000001 Rmu_sc0033228.1_g000001 Rmu_sc0039198.1_g000001 Rmu_sc0042295.1_g000001 Rmu_ssc0000123.1_g000001
rosa_roxburghii	Rroxscaffold_1G00005610 Rroxscaffold_1G00005660 Rroxscaffold_1G00005670 Rroxscaffold_1G00005680 Rroxscaffold_1G00005690 Rroxscaffold_1G00005700 Rroxscaffold_1G00005710 Rroxscaffold_1G00005730 Rroxscaffold_1G00005740 Rroxscaffold_1G00005750 Rroxscaffold_1G00005760 Rroxscaffold_1G00005920 Rroxscaffold_1G00005930 Rroxscaffold_1G00006710 Rroxscaffold_2G00084870 Rroxscaffold_3G00232830 Rroxscaffold_3G00233180 Rroxscaffold_3G00233260 Rroxscaffold_3G00233280 Rroxscaffold_4G00279150 Rroxscaffold_4G00279160
rosa_rugosa	Rorug01G0285800 Rorug01G0285900 Rorug01G0348200 Rorug01G0419100 Rorug01G0419100 Rorug01G0422800 Rorug01G0422900 Rorug05G0435100 Rorug05G0435100 Rorug05G0435100 Rorug05G0435200 Rorug05G0442200 Rorug05G0442200 Rorug05G0442200 Rorug05G0444000 Rorug05G0444100 Rorug05G0444200 Rorug05G0444300 Rorug05G0444400 Rorug05G0444500 Rorug05G0444600 Rorug05G0444700 Rorug05G0444800 Rorug05G0444900 Rorug05G0445000 Rorug05G0445100 Rorug05G0445700.1 Rorug05G0445900.1 Rorug07G0237200
rosa_samantha	Rh1BG318300 Rh1BG402600 Rh1DG349000 Rh1DG432800 Rh5AG498500 Rh5BG519400 Rh5BG521700 Rh5BG521800 Rh5BG521900 Rh5BG522100 Rh5BG522300 Rh5BG522600 Rh5BG522700 Rh5BG523100 Rh5CG543100 Rh5CG545700 Rh5CG545800 Rh5CG545900 Rh5CG546100 Rh5CG546300 Rh5CG546500 Rh5CG547000 Rh5DG525900 Rh5DG535600 Rh7BG368800 Rh7BG368900 Rh7CG387000 Rh7CG387100 Rh7DG379900
rosa_wichuraiana	Rw0G009610 Rw0G011310 Rw1G038910 Rw1G038920 Rw2G049110 Rw5G045620 Rw5G046230 Rw5G046250 Rw5G046260 Rw5G046460 Rw5G046470 Rw5G046480 Rw5G046490 Rw5G046500 Rw5G046510 Rw5G046520 Rw7G019970 Rw7G032250 Rw7G032260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1668
AarI	CACCTGC	1 cut(s)	1567
Acc36I	ACCTGC	3 cut(s)	362, 559, 1567
AccB1I	GGYRCC	1 cut(s)	457
AccB7I	CCANNNNNTGG	2 cut(s)	361, 605
AciI	CCGC	3 cut(s)	566, 743, 1859
AclWI	GGATC	8 cut(s)	40, 133, 508, 1089, 1311, 1385, 1508, 1775
AcoI	YGGCCR	2 cut(s)	731, 1185
AcsI	RAATTY	7 cut(s)	357, 481, 589, 676, 1180, 1450, 1813
AfaI	GTAC	1 cut(s)	184
AfiI	CCNNNNNNNGG	6 cut(s)	227, 361, 605, 1163, 1508, 1726
AgeI	ACCGGT	1 cut(s)	1282
AjnI	CCWGG	4 cut(s)	178, 460, 856, 1156
AloI	GAACNNNNNNTCC	4 cut(s)	373, 405, 421, 453
AluBI	AGCT	7 cut(s)	159, 318, 510, 651, 1222, 1505, 1640
AluI	AGCT	7 cut(s)	159, 318, 510, 651, 1222, 1505, 1640
Alw21I	GWGCWC	2 cut(s)	653, 1698
Alw26I	GTCTC	4 cut(s)	303, 1060, 1165, 1840
AlwI	GGATC	8 cut(s)	40, 133, 508, 1089, 1311, 1385, 1508, 1775
AlwNI	CAGNNNCTG	1 cut(s)	1199
AoxI	GGCC	4 cut(s)	352, 731, 748, 1185
ApeKI	GCWGC	4 cut(s)	98, 1015, 1652, 1861
ApoI	RAATTY	7 cut(s)	357, 481, 589, 676, 1180, 1450, 1813
ArsI	GACNNNNNNTTYG	2 cut(s)	769, 801
AsiGI	ACCGGT	1 cut(s)	1282
Asp700I	GAANNNNTTC	5 cut(s)	253, 710, 843, 912, 1800
AspS9I	GGNCC	5 cut(s)	374, 561, 611, 749, 1151
AsuHPI	GGTGA	2 cut(s)	1272, 1523
AsuII	TTCGAA	1 cut(s)	1804
AvaII	GGWCC	4 cut(s)	374, 561, 611, 1151
BanI	GGYRCC	1 cut(s)	457
BanII	GRGCYC	1 cut(s)	653
BarI	GAAGNNNNNNTAC	4 cut(s)	175, 207, 1629, 1661
Bbv12I	GWGCWC	2 cut(s)	653, 1698
BbvI	GCAGC	3 cut(s)	85, 1027, 1639
BccI	CCATC	4 cut(s)	532, 1042, 1123, 1707
BciT130I	CCWGG	4 cut(s)	180, 462, 858, 1158
BcoDI	GTCTC	4 cut(s)	303, 1060, 1165, 1840
BfuAI	ACCTGC	3 cut(s)	362, 559, 1567
BglII	AGATCT	1 cut(s)	965
BisI	GCNGC	4 cut(s)	99, 1016, 1653, 1862
BlsI	GCNGC	4 cut(s)	100, 1017, 1654, 1863
Bme1390I	CCNGG	4 cut(s)	180, 462, 858, 1158
Bme18I	GGWCC	4 cut(s)	374, 561, 611, 1151
BmgT120I	GGNCC	5 cut(s)	374, 561, 611, 749, 1151
BmiI	GGNNCC	5 cut(s)	459, 562, 563, 1483, 1731
BmrFI	CCNGG	4 cut(s)	180, 462, 858, 1158
BmsI	GCATC	3 cut(s)	837, 927, 1311
BpmI	CTGGAG	1 cut(s)	627
Bpu10I	CCTNAGC	1 cut(s)	1506
Bpu14I	TTCGAA	1 cut(s)	1804
BpuEI	CTTGAG	2 cut(s)	738, 1050
BsaBI	GATNNNNATC	1 cut(s)	271
BsaI	GGTCTC	2 cut(s)	1165, 1840
BsaJI	CCNNGG	3 cut(s)	377, 520, 1487
BsaWI	WCCGGW	1 cut(s)	1282
BsaXI	ACNNNNNCTCC	2 cut(s)	421, 451
Bsc4I	CCNNNNNNNGG	6 cut(s)	227, 361, 605, 1163, 1508, 1726
Bse118I	RCCGGY	2 cut(s)	729, 1282
Bse1I	ACTGG	1 cut(s)	250
Bse3DI	GCAATG	1 cut(s)	770
Bse8I	GATNNNNATC	1 cut(s)	271
BseBI	CCWGG	4 cut(s)	180, 462, 858, 1158
BseDI	CCNNGG	3 cut(s)	377, 520, 1487
BseGI	GGATG	2 cut(s)	828, 1389
BseJI	GATNNNNATC	1 cut(s)	271
BseLI	CCNNNNNNNGG	6 cut(s)	227, 361, 605, 1163, 1508, 1726
BseMI	GCAATG	1 cut(s)	770
BseMII	CTCAG	3 cut(s)	314, 959, 1116
BseNI	ACTGG	1 cut(s)	250
BseRI	GAGGAG	2 cut(s)	363, 442
BseXI	GCAGC	3 cut(s)	85, 1027, 1639
BsgI	GTGCAG	2 cut(s)	1451, 1545
BshFI	GGCC	4 cut(s)	354, 733, 750, 1187
BshNI	GGYRCC	1 cut(s)	457
BshTI	ACCGGT	1 cut(s)	1282
BsiHKAI	GWGCWC	2 cut(s)	653, 1698
BsiSI	CCGG	4 cut(s)	730, 848, 972, 1283
BslFI	GGGAC	3 cut(s)	547, 1003, 1703
BslI	CCNNNNNNNGG	6 cut(s)	227, 361, 605, 1163, 1508, 1726
BsmAI	GTCTC	4 cut(s)	303, 1060, 1165, 1840
BsmFI	GGGAC	3 cut(s)	547, 1003, 1703
BsmI	GAATGC	1 cut(s)	1123
BsnI	GGCC	4 cut(s)	354, 733, 750, 1187
Bso31I	GGTCTC	2 cut(s)	1165, 1840
Bsp119I	TTCGAA	1 cut(s)	1804
Bsp1286I	GDGCHC	2 cut(s)	653, 1698
Bsp143I	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
Bsp19I	CCATGG	1 cut(s)	1487
BspACI	CCGC	3 cut(s)	566, 743, 1859
BspANI	GGCC	4 cut(s)	354, 733, 750, 1187
BspCNI	CTCAG	3 cut(s)	313, 958, 1115
BspHI	TCATGA	1 cut(s)	916
BspLI	GGNNCC	5 cut(s)	459, 562, 563, 1483, 1731
BspMI	ACCTGC	3 cut(s)	362, 559, 1567
BspPI	GGATC	8 cut(s)	40, 133, 508, 1089, 1311, 1385, 1508, 1775
BspT104I	TTCGAA	1 cut(s)	1804
BspT107I	GGYRCC	1 cut(s)	457
BspTNI	GGTCTC	2 cut(s)	1165, 1840
BsrDI	GCAATG	1 cut(s)	770
BsrFI	RCCGGY	2 cut(s)	729, 1282
BsrI	ACTGG	1 cut(s)	250
BssAI	RCCGGY	2 cut(s)	729, 1282
BssECI	CCNNGG	3 cut(s)	377, 520, 1487
BssMI	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
BssT1I	CCWWGG	2 cut(s)	520, 1487
Bst2UI	CCWGG	4 cut(s)	180, 462, 858, 1158
Bst4CI	ACNGT	7 cut(s)	517, 689, 928, 1195, 1260, 1775, 1851
BstBI	TTCGAA	1 cut(s)	1804
BstC8I	GCNNGC	3 cut(s)	731, 1503, 1625
BstDEI	CTNAG	4 cut(s)	300, 945, 1102, 1506
BstDSI	CCRYGG	2 cut(s)	377, 1487
BstF5I	GGATG	2 cut(s)	828, 1389
BstKTI	GATC	9 cut(s)	35, 128, 503, 968, 1084, 1306, 1393, 1516, 1783
BstMAI	GTCTC	4 cut(s)	303, 1060, 1165, 1840
BstMBI	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
BstMWI	GCNNNNNNNGC	3 cut(s)	946, 1228, 1330
BstNI	CCWGG	4 cut(s)	180, 462, 858, 1158
BstNSI	RCATGY	4 cut(s)	178, 1031, 1627, 1652
BstSCI	CCNGG	4 cut(s)	178, 460, 856, 1156
BstV1I	GCAGC	3 cut(s)	85, 1027, 1639
BstX2I	RGATCY	4 cut(s)	125, 500, 965, 1081
BstXI	CCANNNNNNTGG	1 cut(s)	1097
BstYI	RGATCY	4 cut(s)	125, 500, 965, 1081
BsuRI	GGCC	4 cut(s)	354, 733, 750, 1187
BtgI	CCRYGG	2 cut(s)	377, 1487
BtgZI	GCGATG	1 cut(s)	1104
BtsCI	GGATG	2 cut(s)	828, 1389
BtsIMutI	CAGTG	1 cut(s)	1771
BveI	ACCTGC	3 cut(s)	362, 559, 1567
Cac8I	GCNNGC	3 cut(s)	731, 1503, 1625
CaiI	CAGNNNCTG	1 cut(s)	1199
CciI	TCATGA	1 cut(s)	916
Cfr10I	RCCGGY	2 cut(s)	729, 1282
Cfr13I	GGNCC	5 cut(s)	374, 561, 611, 749, 1151
Csp6I	GTAC	1 cut(s)	183
CspAI	ACCGGT	1 cut(s)	1282
CviQI	GTAC	1 cut(s)	183
DdeI	CTNAG	4 cut(s)	300, 945, 1102, 1506
DpnI	GATC	9 cut(s)	34, 127, 502, 967, 1083, 1305, 1392, 1515, 1782
DpnII	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
DraI	TTTAAA	2 cut(s)	865, 1179
EaeI	YGGCCR	2 cut(s)	731, 1185
Ecl136II	GAGCTC	1 cut(s)	651
Eco130I	CCWWGG	2 cut(s)	520, 1487
Eco24I	GRGCYC	1 cut(s)	653
Eco31I	GGTCTC	2 cut(s)	1165, 1840
Eco47I	GGWCC	4 cut(s)	374, 561, 611, 1151
Eco53kI	GAGCTC	1 cut(s)	651
EcoICRI	GAGCTC	1 cut(s)	651
EcoO109I	RGGNCCY	2 cut(s)	561, 749
EcoRI	GAATTC	1 cut(s)	1450
EcoRII	CCWGG	4 cut(s)	178, 460, 856, 1156
EcoT14I	CCWWGG	2 cut(s)	520, 1487
EcoT38I	GRGCYC	1 cut(s)	653
ErhI	CCWWGG	2 cut(s)	520, 1487
FalI	AAGNNNNNCTT	2 cut(s)	897, 929
FaqI	GGGAC	3 cut(s)	547, 1003, 1703
FauI	CCCGC	2 cut(s)	573, 736
FauNDI	CATATG	2 cut(s)	1657, 1753
Fnu4HI	GCNGC	4 cut(s)	99, 1016, 1653, 1862
FokI	GGATG	2 cut(s)	815, 1396
FriOI	GRGCYC	1 cut(s)	653
Fsp4HI	GCNGC	4 cut(s)	99, 1016, 1653, 1862
GluI	GCNGC	4 cut(s)	99, 1016, 1653, 1862
GsuI	CTGGAG	1 cut(s)	627
HaeIII	GGCC	4 cut(s)	354, 733, 750, 1187
HapII	CCGG	4 cut(s)	730, 848, 972, 1283
HinfI	GANTC	5 cut(s)	231, 336, 529, 550, 1716
HpaII	CCGG	4 cut(s)	730, 848, 972, 1283
HphI	GGTGA	2 cut(s)	1272, 1523
Hpy166II	GTNNAC	7 cut(s)	62, 263, 377, 614, 1010, 1072, 1151
Hpy188I	TCNGA	3 cut(s)	880, 1105, 1495
Hpy8I	GTNNAC	7 cut(s)	62, 263, 377, 614, 1010, 1072, 1151
HpyAV	CCTTC	2 cut(s)	158, 231
HpyCH4III	ACNGT	7 cut(s)	517, 689, 928, 1195, 1260, 1775, 1851
HpyCH4IV	ACGT	1 cut(s)	64
HpyF10VI	GCNNNNNNNGC	3 cut(s)	946, 1228, 1330
HpyF3I	CTNAG	4 cut(s)	300, 945, 1102, 1506
HpySE526I	ACGT	1 cut(s)	64
KflI	GGGWCCC	1 cut(s)	561
KroI	GCCGGC	1 cut(s)	729
KroNI	GCCGGC	1 cut(s)	731
Kzo9I	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
LmnI	GCTCC	5 cut(s)	156, 1036, 1345, 1693, 1729
Lsp1109I	GCAGC	3 cut(s)	85, 1027, 1639
LweI	GCATC	3 cut(s)	837, 927, 1311
MaeII	ACGT	1 cut(s)	64
MaeIII	GTNAC	3 cut(s)	337, 1254, 1278
MalI	GATC	9 cut(s)	34, 127, 502, 967, 1083, 1305, 1392, 1515, 1782
MboI	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
MboII	GAAGA	6 cut(s)	12, 38, 275, 851, 975, 1076
MfeI	CAATTG	1 cut(s)	761
MflI	RGATCY	4 cut(s)	125, 500, 965, 1081
MhlI	GDGCHC	2 cut(s)	653, 1698
MlyI	GAGTC	3 cut(s)	240, 345, 1725
MmeI	TCCRAC	1 cut(s)	259
MroNI	GCCGGC	1 cut(s)	729
MroXI	GAANNNNTTC	5 cut(s)	253, 710, 843, 912, 1800
MslI	CAYNNNNRTG	3 cut(s)	1095, 1382, 1830
MspI	CCGG	4 cut(s)	730, 848, 972, 1283
MspR9I	CCNGG	4 cut(s)	180, 462, 858, 1158
MunI	CAATTG	1 cut(s)	761
Mva1269I	GAATGC	1 cut(s)	1123
MvaI	CCWGG	4 cut(s)	180, 462, 858, 1158
MwoI	GCNNNNNNNGC	3 cut(s)	946, 1228, 1330
NaeI	GCCGGC	1 cut(s)	731
NcoI	CCATGG	1 cut(s)	1487
NdeI	CATATG	2 cut(s)	1657, 1753
NdeII	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
NgoMIV	GCCGGC	1 cut(s)	729
NlaIV	GGNNCC	5 cut(s)	459, 562, 563, 1483, 1731
NmuCI	GTSAC	3 cut(s)	337, 1254, 1278
NspI	RCATGY	4 cut(s)	178, 1031, 1627, 1652
NspV	TTCGAA	1 cut(s)	1804
PaeI	GCATGC	1 cut(s)	1627
PagI	TCATGA	1 cut(s)	916
PaqCI	CACCTGC	1 cut(s)	1567
PctI	GAATGC	1 cut(s)	1123
PdiI	GCCGGC	1 cut(s)	731
PdmI	GAANNNNTTC	5 cut(s)	253, 710, 843, 912, 1800
PfeI	GAWTC	2 cut(s)	529, 550
PflMI	CCANNNNNTGG	2 cut(s)	361, 605
PinAI	ACCGGT	1 cut(s)	1282
PkrI	GCNGC	4 cut(s)	100, 1017, 1654, 1863
PleI	GAGTC	3 cut(s)	239, 344, 1724
PpsI	GAGTC	3 cut(s)	239, 344, 1724
PpuMI	RGGWCCY	1 cut(s)	561
PsiI	TTATAA	1 cut(s)	1668
Psp124BI	GAGCTC	1 cut(s)	653
Psp5II	RGGWCCY	1 cut(s)	561
Psp6I	CCWGG	4 cut(s)	178, 460, 856, 1156
PspGI	CCWGG	4 cut(s)	178, 460, 856, 1156
PspN4I	GGNNCC	5 cut(s)	459, 562, 563, 1483, 1731
PspPI	GGNCC	5 cut(s)	374, 561, 611, 749, 1151
PspPPI	RGGWCCY	1 cut(s)	561
PstNI	CAGNNNCTG	1 cut(s)	1199
PsuI	RGATCY	4 cut(s)	125, 500, 965, 1081
RsaI	GTAC	1 cut(s)	184
RsaNI	GTAC	1 cut(s)	183
RseI	CAYNNNNRTG	3 cut(s)	1095, 1382, 1830
SacI	GAGCTC	1 cut(s)	653
SatI	GCNGC	4 cut(s)	99, 1016, 1653, 1862
Sau3AI	GATC	9 cut(s)	32, 125, 500, 965, 1081, 1303, 1390, 1513, 1780
Sau96I	GGNCC	5 cut(s)	374, 561, 611, 749, 1151
SchI	GAGTC	3 cut(s)	240, 345, 1725
ScrFI	CCNGG	4 cut(s)	180, 462, 858, 1158
SduI	GDGCHC	2 cut(s)	653, 1698
SfaNI	GCATC	3 cut(s)	837, 927, 1311
SfuI	TTCGAA	1 cut(s)	1804
SinI	GGWCC	4 cut(s)	374, 561, 611, 1151
SmiMI	CAYNNNNRTG	3 cut(s)	1095, 1382, 1830
SmlI	CTYRAG	2 cut(s)	753, 1065
SmoI	CTYRAG	2 cut(s)	753, 1065
SphI	GCATGC	1 cut(s)	1627
SsiI	CCGC	3 cut(s)	566, 743, 1859
SstI	GAGCTC	1 cut(s)	653
StyD4I	CCNGG	4 cut(s)	178, 460, 856, 1156
StyI	CCWWGG	2 cut(s)	520, 1487
TaaI	ACNGT	7 cut(s)	517, 689, 928, 1195, 1260, 1775, 1851
TaiI	ACGT	1 cut(s)	67
TaqI	TCGA	2 cut(s)	1319, 1804
TfiI	GAWTC	2 cut(s)	529, 550
TscAI	CASTG	1 cut(s)	1778
TseFI	GTSAC	3 cut(s)	337, 1254, 1278
TseI	GCWGC	4 cut(s)	98, 1015, 1652, 1861
Tsp45I	GTSAC	3 cut(s)	337, 1254, 1278
TspGWI	ACGGA	2 cut(s)	45, 394
TspRI	CASTG	1 cut(s)	1778
Van91I	CCANNNNNTGG	2 cut(s)	361, 605
VpaK11BI	GGWCC	4 cut(s)	374, 561, 611, 1151
XapI	RAATTY	7 cut(s)	357, 481, 589, 676, 1180, 1450, 1813
XceI	RCATGY	4 cut(s)	178, 1031, 1627, 1652
XcmI	CCANNNNNNNNNTGG	1 cut(s)	242
XmnI	GAANNNNTTC	5 cut(s)	253, 710, 843, 912, 1800

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.