Rorug05G0444600

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000139
Physical Location & Seq
Reverse (-)
61569889 .. 61578149
8261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0444600.1

Sequence Viewer

Length: 2553 bp
ATGTATGAGTCGGGTCGAGACGTCAAAACGGAGAGGAGAGAAGGCGAAGCCAAACCGATCCGTCAGAGTCTCAACACAGTGATCGGAAAAACAAAAATGGACGACGAATTGGTGGAATTGCAGAAGCAATTCGAGTCGGCACAGCAAGCGAAATCGAGCATCAGATTGTCCGATCGAAACGTCGTCGAATTGGTCCAAAAGCTTCACGAGCTCCACATCATCGACTTCGACCTCCTCCACACCGTCTCCGGCAAAGAATACATCACTCCGGATCAATTGAGGCATGAAATACTGGTGGAGGTCAAGAGGTTGGGCCGTATATCACTGATTGACCTTGCAGATGCTATTGGGGTTGATTTGTACCATGTCGAGAAGCAAGCTCAACATGTGGTTTCTGATGATCCGGGGCTTATGTTGATTCAAGGGGAAATAATATCACAGTCCTATTGGGATTCCGTCGCTGAAGAAATCAATGAGAGGCTTCAAGAATGTAGCCAAGTTGCTTTGGCAGAACTTGCCGCGCAGTTACATGTCAGTTCTGAAATGGTGACATCTGTGTTGGAGCCGCGCCTTGGGACTATGGTGAAAGGTAGGCTTGAAGGTGGGCAATTGTATACCCCTGCTTATGTTGCACGTGTCACTGCCATGGTTCGTGGTGCTGCTAGGGCTATCACAGTTCCCACAAACTTATCAGTCTTGTGGAGTTCTTTACAGCAGTTGCTGCAAGAAATGGAGGGAGCCAGTGGTGTAGCTGTAGAAGGTTCATTTTTCCAATCCCTTTTTAACGGGCTTGTAAAGGAAGGCGAGATTCTTGGATCACTTCGTGCAGGAGTTCATTGGACACCAAATGTTTTTGCCATTGCTCAGAAGGAATCAATTGATTCTTTCTTCTCACAGAACTCTTTCATTAGCTATGACGTTCTGCAGAAGCTTAGAATTCCCCAGCCTGATCAGTTCTTGCAGTCCAGATATCCTGAATGCATACCTTTGGTTACTACATTTATTCACCCATCAATGATTGAGATGCTTGATGCTGCTATAGAAGATGCTCTTGAGCGTGGGAGCTGGATGGATTCTCTTTCTATACTGCCAATGTCCTTTGGATCTCAAGATGCATCCAAACTTTTGTCCCTTTGTCCTTCCATTCAGCAGGGCCTCAAGTCTGATAAGGCAATAATCTTCGGGGAATCCTTTGTATTTAGCAGTGCCTTTATCAAGGATGTGTACGATCGCCTGGAGAAAGAAATGGAAACCTTTATAGTTTCGAGTACTTCTGGTACTATGGTGTCTGATGATTTGCAGGAGACAAAAGTTGGTCATGAAACAAGCAGGTTCACCGAATCAAATGAAACGGTTAGTGACAGCAGCAGTAATAAACAGACCATGGAGAAAGGATCAAAGAAGAAAAAGGGTCGAGCGGCTGGAAATATGGGAGCAGGTGCGGCCGAAGGTGATCCGGAAAACCAGGACTCTGTTCCTACAAAATCTAAGAAAAACCAAAGGAAAGGCAAGAATTCCTCCTCCGCACAAGTTGCTGACTCAAAAGCATCTGCTAAGTTGGTAAAATCTAAAGAGGAAAATCTGAACATTCCTTCAGAAGATTGGATGATGAACAAGATTGCAACACTGGTTCCTGAATTCGAAGAACAAGGTCTTGATGATCCACAAACAATTATTAGACCTTTGGCAAACTATGTGAGACCTATGCTAATAAACTCTTGGAAGGAGAGAAGAAAGGCATTGTTTACTGAAAATGCAGAGAGAATGAAGCACTTGCTTGATAATTTGCAAAAGAAACTTGATGAGTCTTTCTTAAACATGCAGCTCTACGAAAAAGCTTTAGATTTGTTTGAAGATGACCCATCAACTTCAGTCATTTTGCACCGGCATTTGTTAAGAACAACAGCTACTACTATTGTGGATATGCTTCTTCTTAATTTGGACATGCACAACAAATTGAAGAATGGAGTTGAAGTTGAGGACACTCAGATTTCAGAATCTTCTTCACTTAACCCAGGAGAAAGAACTTCCATTGCTAAGAATTTTCCTGGATCTCTCTCAAAGAAGGCTCTTGTTGTTGTTGAAGCCTTGGAAGGAAAGCGGGTGGAAACATTCATGACTGCTTTAAGAGACATAGCTGAAGAAAGTGGTTTACTCCTGAAAAAGCTTGACAAGAAGTTGGAAAGAACACTTCTGCATTCGTATCAAAAGGATTTGGCGTCTCAAGTTTCTGCTGAAATGGATCCAGTCTCTGTTTTGGCCAAAGTTGTTTCCCTGCTATATGTACAGATTCACCATAAAGCTCTCCAAGCACCTGGAAGGGCCATCGCTGTTGCTGTTTCTCGGCTGAAGGATAAACTAGATGAATCAGCTTTCAAGATCCTGACAGAATATCAAACTGCAACAGTAACCCTCTTAGCACTAATATCCGCTGCAAGTGGAGATGAAGAAGACTGTACATCTGATAGAATATTGAGCAAAAGGGAGCTCCTGGAAAATCAAATGCCTGCACTGAAAGGCCTGGTTTTGCGCACTGGCACCTCACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_1

Protein Analysis

850

Amino Acids

94.45

Weight (kDa)

5.43

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E3_UFM1_ligase PF09743 36 - 320 6.1e-100 E3 UFM1-protein ligase 1
WHD_UFL1_5th PF25870 337 - 384 1.4e-09 Winged helix-turn-helix domain 5 in UFL1 protein
UFL1 PF23659 593 - 723 1.4e-39 E3 UFM1-protein ligase 1-like domain
UFL1_C PF25041 728 - 841 1.5e-29 E3 UFM1-protein ligase 1 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000139)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g08100 FvH4_1g08100 FvH4_3g41771 FvH4_3g42301 FvH4_3g42302 FvH4_3g42303 FvH4_3g42304 FvH4_3g42321 FvH4_3g42360 FvH4_3g42420 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42450 FvH4_3g42460 FvH4_3g42470 FvH4_3g42490 FvH4_3g42581 FvH4_3g42582 FvH4_5g30990 FvH4_5g30990 FvH4_5g37874 FvH4_6g47401
prunus_persica Prupe.2G278400_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.2G278600_v2.0.a1 Prupe.6G215000_v2.0.a1
pyrus_communis pycom15g26480
rosa_chinensis RchiOBHm_Chr1g0361131 RchiOBHm_Chr1g0380591 RchiOBHm_Chr1g0380601 RchiOBHm_Chr5g0075661 RchiOBHm_Chr5g0075691 RchiOBHm_Chr5g0075921 RchiOBHm_Chr5g0075931 RchiOBHm_Chr5g0075941 RchiOBHm_Chr5g0076031 RchiOBHm_Chr5g0076061 RchiOBHm_Chr5g0076091 RchiOBHm_Chr5g0076101 RchiOBHm_Chr5g0076131 RchiOBHm_Chr5g0076141 RchiOBHm_Chr5g0076151 RchiOBHm_Chr7g0226061 RchiOBHm_Chr7g0226071 RchiOBHm_Chr7g0226091 RchiOBHm_Chr7g0226431 RchiOBHm_Chr7g0226441
rosa_laevigata RLG00000027157 RLG00000036604
rosa_multiflora Rmu_co8028714.1_g000001 Rmu_co8069518.1_g000001 Rmu_co8225880.1_g000001 Rmu_co8266415.1_g000001 Rmu_sc0001470.1_g000003 Rmu_sc0001470.1_g000004 Rmu_sc0001764.1_g000007 Rmu_sc0002627.1_g000001 Rmu_sc0002652.1_g000008 Rmu_sc0002652.1_g000010 Rmu_sc0002652.1_g000011 Rmu_sc0002652.1_g000013 Rmu_sc0002652.1_g000016 Rmu_sc0002820.1_g000003 Rmu_sc0002820.1_g000004 Rmu_sc0002863.1_g000037 Rmu_sc0003016.1_g000001 Rmu_sc0003601.1_g000001 Rmu_sc0003945.1_g000010 Rmu_sc0004200.1_g000005 Rmu_sc0004250.1_g000018 Rmu_sc0004647.1_g000006 Rmu_sc0004647.1_g000007 Rmu_sc0005762.1_g000005 Rmu_sc0005762.1_g000011 Rmu_sc0005762.1_g000014 Rmu_sc0005961.1_g000010 Rmu_sc0007791.1_g000001 Rmu_sc0007791.1_g000006 Rmu_sc0007791.1_g000010 Rmu_sc0008955.1_g000006 Rmu_sc0008955.1_g000008 Rmu_sc0008955.1_g000019 Rmu_sc0008955.1_g000021 Rmu_sc0010684.1_g000002 Rmu_sc0012777.1_g000003 Rmu_sc0014532.1_g000001 Rmu_sc0018126.1_g000001 Rmu_sc0021483.1_g000001 Rmu_sc0025529.1_g000001 Rmu_sc0027085.1_g000001 Rmu_sc0027085.1_g000003 Rmu_sc0028007.1_g000001 Rmu_sc0031697.1_g000001 Rmu_sc0033228.1_g000001 Rmu_sc0039198.1_g000001 Rmu_sc0042295.1_g000001 Rmu_ssc0000123.1_g000001
rosa_roxburghii Rroxscaffold_1G00005610 Rroxscaffold_1G00005660 Rroxscaffold_1G00005670 Rroxscaffold_1G00005680 Rroxscaffold_1G00005690 Rroxscaffold_1G00005700 Rroxscaffold_1G00005710 Rroxscaffold_1G00005730 Rroxscaffold_1G00005740 Rroxscaffold_1G00005750 Rroxscaffold_1G00005760 Rroxscaffold_1G00005920 Rroxscaffold_1G00005930 Rroxscaffold_1G00006710 Rroxscaffold_2G00084870 Rroxscaffold_3G00232830 Rroxscaffold_3G00233180 Rroxscaffold_3G00233260 Rroxscaffold_3G00233280 Rroxscaffold_4G00279150 Rroxscaffold_4G00279160
rosa_rugosa Rorug01G0285800 Rorug01G0285900 Rorug01G0348200 Rorug01G0419100 Rorug01G0419100 Rorug01G0422800 Rorug01G0422900 Rorug05G0435100 Rorug05G0435100 Rorug05G0435100 Rorug05G0435200 Rorug05G0442200 Rorug05G0442200 Rorug05G0442200 Rorug05G0444000 Rorug05G0444100 Rorug05G0444200 Rorug05G0444300 Rorug05G0444400 Rorug05G0444500 Rorug05G0444600 Rorug05G0444700 Rorug05G0444800 Rorug05G0444900 Rorug05G0445000 Rorug05G0445100 Rorug05G0445700.1 Rorug05G0445900.1 Rorug07G0237200
rosa_samantha Rh1BG318300 Rh1BG402600 Rh1DG349000 Rh1DG432800 Rh5AG498500 Rh5BG519400 Rh5BG521700 Rh5BG521800 Rh5BG521900 Rh5BG522100 Rh5BG522300 Rh5BG522600 Rh5BG522700 Rh5BG523100 Rh5CG543100 Rh5CG545700 Rh5CG545800 Rh5CG545900 Rh5CG546100 Rh5CG546300 Rh5CG546500 Rh5CG547000 Rh5DG525900 Rh5DG535600 Rh7BG368800 Rh7BG368900 Rh7CG387000 Rh7CG387100 Rh7DG379900
rosa_wichuraiana Rw0G009610 Rw0G011310 Rw1G038910 Rw1G038920 Rw2G049110 Rw5G045620 Rw5G046230 Rw5G046250 Rw5G046260 Rw5G046460 Rw5G046470 Rw5G046480 Rw5G046490 Rw5G046500 Rw5G046510 Rw5G046520 Rw7G019970 Rw7G032250 Rw7G032260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1427
AatII GACGTC 1 cut(s) 24
Acc16I TGCGCA 1 cut(s) 2531
Acc36I ACCTGC 2 cut(s) 1320, 1427
AccB1I GGYRCC 1 cut(s) 2537
AccBSI CCGCTC 1 cut(s) 1418
AccI GTMKAC 1 cut(s) 614
AccII CGCG 2 cut(s) 521, 568
AccIII TCCGGA 2 cut(s) 268, 1456
AciI CCGC 7 cut(s) 519, 566, 1418, 1442, 1524, 2101, 2430
AcoI YGGCCR 2 cut(s) 1443, 2259
AcsI RAATTY 4 cut(s) 936, 1513, 1637, 2041
AcuI CTGAAG 5 cut(s) 483, 1578, 1854, 2160, 2369
AcvI CACGTG 1 cut(s) 635
AcyI GRCGYC 2 cut(s) 21, 2219
AdeI CACNNNGTG 1 cut(s) 824
AfaI GTAC 6 cut(s) 362, 1226, 1270, 1279, 2286, 2458
AfiI CCNNNNNNNGG 1 cut(s) 572
AflIII ACRYGT 3 cut(s) 385, 529, 634
AgsI TTSAA 8 cut(s) 422, 485, 599, 1853, 1960, 1973, 2084, 2377
AjnI CCWGG 7 cut(s) 1233, 1464, 2014, 2047, 2314, 2490, 2520
Alw21I GWGCWC 2 cut(s) 213, 2490
Alw26I GTCTC 8 cut(s) 12, 74, 250, 1298, 1693, 2124, 2226, 2254
AlwNI CAGNNNCTG 2 cut(s) 721, 2252
Aor13HI TCCGGA 2 cut(s) 268, 1456
AoxI GGCC 6 cut(s) 313, 1153, 1443, 2259, 2322, 2518
ApeKI GCWGC 6 cut(s) 659, 721, 1034, 1365, 1822, 2432
ApoI RAATTY 4 cut(s) 936, 1513, 1637, 2041
Asp700I GAANNNNTTC 2 cut(s) 128, 902
AspLEI GCGC 3 cut(s) 523, 570, 2532
AspS9I GGNCC 4 cut(s) 193, 313, 1153, 2322
AsuC2I CCSGG 1 cut(s) 405
AsuHPI GGTGA 6 cut(s) 559, 595, 998, 1327, 1463, 2285
AsuII TTCGAA 1 cut(s) 1641
AvaII GGWCC 1 cut(s) 193
BaeI ACNNNNGTAYC 2 cut(s) 1269, 1302
BalI TGGCCA 1 cut(s) 2261
BamHI GGATCC 1 cut(s) 2242
BanI GGYRCC 1 cut(s) 2537
BanII GRGCYC 2 cut(s) 213, 2490
BauI CACGAG 1 cut(s) 206
BbrPI CACGTG 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 2457
Bbv12I GWGCWC 2 cut(s) 213, 2490
BbvI GCAGC 6 cut(s) 646, 708, 1021, 1377, 1834, 2419
BccI CCATC 4 cut(s) 1018, 1063, 1870, 2333
BceAI ACGGC 1 cut(s) 300
BciT130I CCWGG 7 cut(s) 1235, 1466, 2016, 2049, 2316, 2492, 2522
BclI TGATCA 1 cut(s) 949
BcnI CCSGG 1 cut(s) 405
BcoDI GTCTC 8 cut(s) 12, 74, 250, 1298, 1693, 2124, 2226, 2254
BfaI CTAG 2 cut(s) 663, 2360
BfmI CTRYAG 3 cut(s) 753, 923, 1038
BfuAI ACCTGC 2 cut(s) 1320, 1427
BmcAI AGTACT 1 cut(s) 1270
Bme1390I CCNGG 8 cut(s) 405, 1235, 1466, 2016, 2049, 2316, 2492, 2522
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 4 cut(s) 193, 313, 1153, 2322
BmiI GGNNCC 5 cut(s) 564, 739, 1632, 2244, 2539
BmrFI CCNGG 8 cut(s) 405, 1235, 1466, 2016, 2049, 2316, 2492, 2522
BmsI GCATC 8 cut(s) 168, 331, 1014, 1021, 1036, 1102, 1124, 1556
BpiI GAAGAC 1 cut(s) 2457
BpmI CTGGAG 1 cut(s) 1256
Bpu14I TTCGAA 1 cut(s) 1641
BpuEI CTTGAG 4 cut(s) 1073, 1092, 1142, 2208
BpuMI CCSGG 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 635
BsaHI GRCGYC 2 cut(s) 21, 2219
BsaI GGTCTC 1 cut(s) 1693
BsaJI CCNNGG 6 cut(s) 404, 571, 645, 1383, 2014, 2088
BsaWI WCCGGW 2 cut(s) 268, 1456
BsaXI ACNNNNNCTCC 4 cut(s) 230, 260, 1959, 1989
Bsc4I CCNNNNNNNGG 1 cut(s) 572
Bse118I RCCGGY 1 cut(s) 1884
Bse1I ACTGG 5 cut(s) 297, 741, 1632, 2246, 2539
Bse3DI GCAATG 2 cut(s) 858, 2031
BseAI TCCGGA 2 cut(s) 268, 1456
BseBI CCWGG 7 cut(s) 1235, 1466, 2016, 2049, 2316, 2492, 2522
BseDI CCNNGG 6 cut(s) 404, 571, 645, 1383, 2014, 2088
BseGI GGATG 4 cut(s) 1074, 1115, 1225, 1611
BseLI CCNNNNNNNGG 1 cut(s) 572
BseMI GCAATG 2 cut(s) 858, 2031
BseMII CTCAG 2 cut(s) 878, 2000
BseNI ACTGG 5 cut(s) 297, 741, 1632, 2246, 2539
BseRI GAGGAG 3 cut(s) 49, 224, 1510
BseX3I CGGCCG 1 cut(s) 1443
BseXI GCAGC 6 cut(s) 646, 708, 1021, 1377, 1834, 2419
BseYI CCCAGC 1 cut(s) 942
BsgI GTGCAG 2 cut(s) 846, 2493
Bsh1236I CGCG 2 cut(s) 521, 568
Bsh1285I CGRYCG 3 cut(s) 175, 1231, 1446
BshFI GGCC 6 cut(s) 315, 1155, 1445, 2261, 2324, 2520
BshNI GGYRCC 1 cut(s) 2537
BsiEI CGRYCG 3 cut(s) 175, 1231, 1446
BsiHKAI GWGCWC 2 cut(s) 213, 2490
BsiSI CCGG 5 cut(s) 249, 269, 404, 1457, 1885
BslFI GGGAC 2 cut(s) 589, 1114
BslI CCNNNNNNNGG 1 cut(s) 572
BsmAI GTCTC 8 cut(s) 12, 74, 250, 1298, 1693, 2124, 2226, 2254
BsmBI CGTCTC 3 cut(s) 12, 250, 2226
BsmFI GGGAC 2 cut(s) 589, 1114
BsmI GAATGC 2 cut(s) 983, 2197
BsnI GGCC 6 cut(s) 315, 1155, 1445, 2261, 2324, 2520
Bso31I GGTCTC 1 cut(s) 1693
Bsp119I TTCGAA 1 cut(s) 1641
Bsp1286I GDGCHC 2 cut(s) 213, 2490
Bsp13I TCCGGA 2 cut(s) 268, 1456
Bsp1407I TGTACA 2 cut(s) 2284, 2456
Bsp19I CCATGG 2 cut(s) 645, 1383
BspACI CCGC 7 cut(s) 519, 566, 1418, 1442, 1524, 2101, 2430
BspANI GGCC 6 cut(s) 315, 1155, 1445, 2261, 2324, 2520
BspCNI CTCAG 2 cut(s) 877, 1999
BspEI TCCGGA 2 cut(s) 268, 1456
BspFNI CGCG 2 cut(s) 521, 568
BspHI TCATGA 2 cut(s) 1318, 2115
BspLI GGNNCC 5 cut(s) 564, 739, 1632, 2244, 2539
BspMAI CTGCAG 1 cut(s) 927
BspMI ACCTGC 2 cut(s) 1320, 1427
BspT104I TTCGAA 1 cut(s) 1641
BspT107I GGYRCC 1 cut(s) 2537
BspTNI GGTCTC 1 cut(s) 1693
BsrBI CCGCTC 1 cut(s) 1418
BsrDI GCAATG 2 cut(s) 858, 2031
BsrFI RCCGGY 1 cut(s) 1884
BsrGI TGTACA 2 cut(s) 2284, 2456
BsrI ACTGG 5 cut(s) 297, 741, 1632, 2246, 2539
BssAI RCCGGY 1 cut(s) 1884
BssECI CCNNGG 6 cut(s) 404, 571, 645, 1383, 2014, 2088
BssNAI GTATAC 1 cut(s) 615
BssNI GRCGYC 2 cut(s) 21, 2219
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 4 cut(s) 571, 645, 1383, 2088
Bst1107I GTATAC 1 cut(s) 615
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 7 cut(s) 1235, 1466, 2016, 2049, 2316, 2492, 2522
Bst4CI ACNGT 7 cut(s) 79, 244, 441, 676, 1354, 2407, 2456
BstACI GRCGYC 2 cut(s) 21, 2219
BstAPI GCANNNNNTGC 3 cut(s) 515, 721, 1532
BstAUI TGTACA 2 cut(s) 2284, 2456
BstBAI YACGTR 1 cut(s) 635
BstBI TTCGAA 1 cut(s) 1641
BstC8I GCNNGC 3 cut(s) 147, 378, 2508
BstDEI CTNAG 7 cut(s) 864, 932, 1488, 1554, 1986, 2037, 2416
BstDSI CCRYGG 2 cut(s) 645, 1383
BstF5I GGATG 4 cut(s) 1074, 1115, 1225, 1611
BstFNI CGCG 2 cut(s) 521, 568
BstHHI GCGC 3 cut(s) 523, 570, 2532
BstMAI GTCTC 8 cut(s) 12, 74, 250, 1298, 1693, 2124, 2226, 2254
BstMCI CGRYCG 3 cut(s) 175, 1231, 1446
BstMWI GCNNNNNNNGC 9 cut(s) 146, 208, 515, 629, 665, 721, 1442, 1532, 2309
BstNI CCWGG 7 cut(s) 1235, 1466, 2016, 2049, 2316, 2492, 2522
BstNSI RCATGY 4 cut(s) 389, 533, 1822, 1948
BstSCI CCNGG 8 cut(s) 403, 1233, 1464, 2014, 2047, 2314, 2490, 2520
BstSFI CTRYAG 3 cut(s) 753, 923, 1038
BstUI CGCG 2 cut(s) 521, 568
BstV1I GCAGC 6 cut(s) 646, 708, 1021, 1377, 1834, 2419
BstV2I GAAGAC 1 cut(s) 2457
BstX2I RGATCY 4 cut(s) 1103, 2051, 2242, 2379
BstXI CCANNNNNNTGG 1 cut(s) 2315
BstYI RGATCY 4 cut(s) 1103, 2051, 2242, 2379
BstZ17I GTATAC 1 cut(s) 615
BstZI CGGCCG 1 cut(s) 1443
BsuRI GGCC 6 cut(s) 315, 1155, 1445, 2261, 2324, 2520
BtgI CCRYGG 2 cut(s) 645, 1383
BtgZI GCGATG 1 cut(s) 2311
BtsCI GGATG 4 cut(s) 1074, 1115, 1225, 1611
BtsI GCAGTG 2 cut(s) 639, 1210
BtsIMutI CAGTG 8 cut(s) 84, 323, 639, 748, 1210, 1625, 2510, 2532
BveI ACCTGC 2 cut(s) 1320, 1427
Cac8I GCNNGC 3 cut(s) 147, 378, 2508
CaiI CAGNNNCTG 2 cut(s) 721, 2252
CciI TCATGA 2 cut(s) 1318, 2115
CfoI GCGC 3 cut(s) 523, 570, 2532
Cfr10I RCCGGY 1 cut(s) 1884
Cfr13I GGNCC 4 cut(s) 193, 313, 1153, 2322
CseI GACGC 1 cut(s) 2208
Csp6I GTAC 6 cut(s) 361, 1225, 1269, 1278, 2285, 2457
CviQI GTAC 6 cut(s) 361, 1225, 1269, 1278, 2285, 2457
DdeI CTNAG 7 cut(s) 864, 932, 1488, 1554, 1986, 2037, 2416
DraIII CACNNNGTG 1 cut(s) 824
EaeI YGGCCR 2 cut(s) 1443, 2259
EagI CGGCCG 1 cut(s) 1443
Ecl136II GAGCTC 2 cut(s) 211, 2488
EclXI CGGCCG 1 cut(s) 1443
Eco130I CCWWGG 4 cut(s) 571, 645, 1383, 2088
Eco147I AGGCCT 1 cut(s) 2520
Eco24I GRGCYC 2 cut(s) 213, 2490
Eco31I GGTCTC 1 cut(s) 1693
Eco32I GATATC 1 cut(s) 971
Eco47I GGWCC 1 cut(s) 193
Eco52I CGGCCG 1 cut(s) 1443
Eco53kI GAGCTC 2 cut(s) 211, 2488
Eco57I CTGAAG 5 cut(s) 483, 1578, 1854, 2160, 2369
Eco72I CACGTG 1 cut(s) 635
EcoICRI GAGCTC 2 cut(s) 211, 2488
EcoO109I RGGNCCY 1 cut(s) 1153
EcoRI GAATTC 3 cut(s) 936, 1513, 1637
EcoRII CCWGG 7 cut(s) 1233, 1464, 2014, 2047, 2314, 2490, 2520
EcoRV GATATC 1 cut(s) 971
EcoT14I CCWWGG 4 cut(s) 571, 645, 1383, 2088
EcoT22I ATGCAT 2 cut(s) 983, 1117
EcoT38I GRGCYC 2 cut(s) 213, 2490
ErhI CCWWGG 4 cut(s) 571, 645, 1383, 2088
Esp3I CGTCTC 3 cut(s) 12, 250, 2226
FalI AAGNNNNNCTT 4 cut(s) 579, 611, 1035, 1067
FaqI GGGAC 2 cut(s) 589, 1114
FauI CCCGC 1 cut(s) 2094
FbaI TGATCA 1 cut(s) 949
FblI GTMKAC 1 cut(s) 614
FokI GGATG 4 cut(s) 1081, 1102, 1232, 1618
FriOI GRGCYC 2 cut(s) 213, 2490
FspBI CTAG 2 cut(s) 663, 2360
FspI TGCGCA 1 cut(s) 2531
GlaI GCGC 3 cut(s) 522, 569, 2531
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 1 cut(s) 1256
HaeIII GGCC 6 cut(s) 315, 1155, 1445, 2261, 2324, 2520
HapII CCGG 5 cut(s) 249, 269, 404, 1457, 1885
HgaI GACGC 1 cut(s) 2208
HhaI GCGC 3 cut(s) 523, 570, 2532
Hin1I GRCGYC 2 cut(s) 21, 2219
Hin6I GCGC 3 cut(s) 521, 568, 2530
HinP1I GCGC 3 cut(s) 521, 568, 2530
HindIII AAGCTT 4 cut(s) 200, 929, 1836, 2165
HpaII CCGG 5 cut(s) 249, 269, 404, 1457, 1885
HphI GGTGA 6 cut(s) 559, 595, 998, 1327, 1463, 2285
Hpy166II GTNNAC 5 cut(s) 615, 1225, 1335, 1746, 2153
Hpy8I GTNNAC 5 cut(s) 615, 1225, 1335, 1746, 2153
Hpy99I CGWCG 4 cut(s) 107, 185, 188, 461
HpyCH4III ACNGT 7 cut(s) 79, 244, 441, 676, 1354, 2407, 2456
HpyCH4IV ACGT 4 cut(s) 21, 180, 634, 918
HpyF10VI GCNNNNNNNGC 9 cut(s) 146, 208, 515, 629, 665, 721, 1442, 1532, 2309
HpyF3I CTNAG 7 cut(s) 864, 932, 1488, 1554, 1986, 2037, 2416
HpySE526I ACGT 4 cut(s) 21, 180, 634, 918
Hsp92I GRCGYC 2 cut(s) 21, 2219
HspAI GCGC 3 cut(s) 521, 568, 2530
Kpn2I TCCGGA 2 cut(s) 268, 1456
Ksp22I TGATCA 1 cut(s) 949
LmnI GCTCC 7 cut(s) 216, 562, 737, 1062, 1433, 2485, 2493
Lsp1109I GCAGC 6 cut(s) 646, 708, 1021, 1377, 1834, 2419
LweI GCATC 8 cut(s) 168, 331, 1014, 1021, 1036, 1102, 1124, 1556
MaeI CTAG 2 cut(s) 663, 2360
MaeII ACGT 4 cut(s) 21, 180, 634, 918
MaeIII GTNAC 6 cut(s) 525, 547, 637, 991, 1358, 2407
MbiI CCGCTC 1 cut(s) 1418
MfeI CAATTG 3 cut(s) 275, 608, 876
MflI RGATCY 4 cut(s) 1103, 2051, 2242, 2379
MhlI GDGCHC 2 cut(s) 213, 2490
MlsI TGGCCA 1 cut(s) 2261
MluNI TGGCCA 1 cut(s) 2261
MlyI GAGTC 6 cut(s) 17, 76, 143, 1463, 1532, 1814
MmeI TCCRAC 2 cut(s) 540, 2160
Mox20I TGGCCA 1 cut(s) 2261
Mph1103I ATGCAT 2 cut(s) 983, 1117
MroI TCCGGA 2 cut(s) 268, 1456
MroXI GAANNNNTTC 2 cut(s) 128, 902
MscI TGGCCA 1 cut(s) 2261
MseI TTAA 6 cut(s) 783, 1814, 1895, 1935, 2010, 2126
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 2261
MspA1I CMGCKG 1 cut(s) 2432
MspI CCGG 5 cut(s) 249, 269, 404, 1457, 1885
MspR9I CCNGG 8 cut(s) 405, 1235, 1466, 2016, 2049, 2316, 2492, 2522
MunI CAATTG 3 cut(s) 275, 608, 876
Mva1269I GAATGC 2 cut(s) 983, 2197
MvaI CCWGG 7 cut(s) 1235, 1466, 2016, 2049, 2316, 2492, 2522
MvnI CGCG 2 cut(s) 521, 568
MwoI GCNNNNNNNGC 9 cut(s) 146, 208, 515, 629, 665, 721, 1442, 1532, 2309
NciI CCSGG 1 cut(s) 405
NcoI CCATGG 2 cut(s) 645, 1383
NlaIV GGNNCC 5 cut(s) 564, 739, 1632, 2244, 2539
NmeAIII GCCGAG 1 cut(s) 2323
NmuCI GTSAC 3 cut(s) 547, 637, 1358
NsbI TGCGCA 1 cut(s) 2531
NsiI ATGCAT 2 cut(s) 983, 1117
NspI RCATGY 4 cut(s) 389, 533, 1822, 1948
NspV TTCGAA 1 cut(s) 1641
PagI TCATGA 2 cut(s) 1318, 2115
PaqCI CACCTGC 1 cut(s) 1427
PceI AGGCCT 1 cut(s) 2520
PciI ACATGT 2 cut(s) 385, 529
PctI GAATGC 2 cut(s) 983, 2197
PdmI GAANNNNTTC 2 cut(s) 128, 902
PfoI TCCNGGA 2 cut(s) 2047, 2490
Ple19I CGATCG 2 cut(s) 175, 1231
PleI GAGTC 6 cut(s) 16, 75, 142, 1463, 1532, 1813
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
PpsI GAGTC 6 cut(s) 16, 75, 142, 1463, 1532, 1813
Ppu21I YACGTR 1 cut(s) 635
PscI ACATGT 2 cut(s) 385, 529
Psp124BI GAGCTC 2 cut(s) 213, 2490
Psp6I CCWGG 7 cut(s) 1233, 1464, 2014, 2047, 2314, 2490, 2520
PspCI CACGTG 1 cut(s) 635
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 7 cut(s) 1233, 1464, 2014, 2047, 2314, 2490, 2520
PspN4I GGNNCC 5 cut(s) 564, 739, 1632, 2244, 2539
PspPI GGNCC 4 cut(s) 193, 313, 1153, 2322
PsrI GAACNNNNNNTAC 2 cut(s) 1891, 1923
PstI CTGCAG 1 cut(s) 927
PstNI CAGNNNCTG 2 cut(s) 721, 2252
PsuI RGATCY 4 cut(s) 1103, 2051, 2242, 2379
PvuI CGATCG 2 cut(s) 175, 1231
RsaI GTAC 6 cut(s) 362, 1226, 1270, 1279, 2286, 2458
RsaNI GTAC 6 cut(s) 361, 1225, 1269, 1278, 2285, 2457
RseI CAYNNNNRTG 1 cut(s) 644
SacI GAGCTC 2 cut(s) 213, 2490
SaqAI TTAA 6 cut(s) 783, 1814, 1895, 1935, 2010, 2126
Sau96I GGNCC 4 cut(s) 193, 313, 1153, 2322
ScaI AGTACT 1 cut(s) 1270
SchI GAGTC 6 cut(s) 17, 76, 143, 1463, 1532, 1814
ScrFI CCNGG 8 cut(s) 405, 1235, 1466, 2016, 2049, 2316, 2492, 2522
SduI GDGCHC 2 cut(s) 213, 2490
SfaNI GCATC 8 cut(s) 168, 331, 1014, 1021, 1036, 1102, 1124, 1556
SfcI CTRYAG 3 cut(s) 753, 923, 1038
SfuI TTCGAA 1 cut(s) 1641
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 1 cut(s) 644
SmlI CTYRAG 4 cut(s) 1052, 1107, 1157, 2223
SmoI CTYRAG 4 cut(s) 1052, 1107, 1157, 2223
SseBI AGGCCT 1 cut(s) 2520
SsiI CCGC 7 cut(s) 519, 566, 1418, 1442, 1524, 2101, 2430
SspI AATATT 1 cut(s) 2472
SspMI CTAG 2 cut(s) 663, 2360
SstI GAGCTC 2 cut(s) 213, 2490
StuI AGGCCT 1 cut(s) 2520
StyD4I CCNGG 8 cut(s) 403, 1233, 1464, 2014, 2047, 2314, 2490, 2520
StyI CCWWGG 4 cut(s) 571, 645, 1383, 2088
TaaI ACNGT 7 cut(s) 79, 244, 441, 676, 1354, 2407, 2456
TaiI ACGT 4 cut(s) 24, 183, 637, 921
TatI WGTACW 3 cut(s) 1268, 2284, 2456
TauI GCSGC 4 cut(s) 521, 568, 1421, 1445
Tru1I TTAA 6 cut(s) 783, 1814, 1895, 1935, 2010, 2126
Tru9I TTAA 6 cut(s) 783, 1814, 1895, 1935, 2010, 2126
TscAI CASTG 8 cut(s) 84, 330, 646, 748, 1210, 1632, 2517, 2539
TseFI GTSAC 3 cut(s) 547, 637, 1358
TseI GCWGC 6 cut(s) 659, 721, 1034, 1365, 1822, 2432
Tsp45I GTSAC 3 cut(s) 547, 637, 1358
TspGWI ACGGA 3 cut(s) 44, 50, 445
TspRI CASTG 8 cut(s) 84, 330, 646, 748, 1210, 1632, 2517, 2539
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 4 cut(s) 936, 1513, 1637, 2041
XceI RCATGY 4 cut(s) 389, 533, 1822, 1948
XmiI GTMKAC 1 cut(s) 614
XmnI GAANNNNTTC 2 cut(s) 128, 902
XspI CTAG 2 cut(s) 663, 2360
ZraI GACGTC 1 cut(s) 22
ZrmI AGTACT 1 cut(s) 1270
Zsp2I ATGCAT 2 cut(s) 983, 1117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.