Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0002707

Physical Location & Seq

Genomic Coordinates Forward (+)

2688676 .. 2693051

Gene Structure 4376 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00159490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGGCATGGGATTTTGTCGTGTCCGCTTTGTCAAATTTAACTTTGAATTTAGGTCGATTTGGATATGAATTTAAGACCTCGGTAAATCGGAGAAAAGGCTCGAGCATCAGACGAGATACCCTTCCTCTCTCTTATAAACGAGTCCGTTTGAAATTGGATTCCGGGAATACCACCAAAATAAAACCAGCTTGTATTAAAAATGACATAATCTCAATTAAGAAAATCCGCGGTTGCTCTGCTAGGGCAACGTGGGTTTTGCTGCCATCCTCCACCGGAATCTTCAAAGAATCAAGGTCAAATCCGGTAGCCATGTCTAGGGCTCGAGTCTACGCCGACGTCAACGTTCAGCGCCCCAGAGATTACTGGGATTACGAATCTTTCGTCCTTCAATGGGGCGTTCAAGATGAATATGAGGTTGTTCGGAAGGTTGGAAGAGGAAAGTACAGTGAGGTCTTCGAGGGTATAAATGTCACCAACAATGAAAAATGCGTGATCAAGATCCTTAAACCAGTCAAGAAAAAGAAGATTAAAAGGGAGATAAAAATATTGCAGAATCTTTGTGGAGGTCCAAATATTGTGAAGCTGCTTGATATTGTCAGGGATGAGCTCTCAAAAACTCCTAGTTTAATCTTTGAACATGTCAACAGTACAGATTTCAAAGTGTTGTACCCCACACTGACAGATTACGACATTCGCTACTATATATATGAACTTCTCAAGGCATTGGATTACTGTCATTCCCAGGGCATAATGCATCGAGATGTGAAGCCTCACAACGTTATGATTGATCACGAGTTGCGGAAACTTCGCCTGATTGATTGGGGTCTTGCTGAATTCTACCATCCTGGCAAAGAATACAATGTCCGAGTAGCTTCAAGGCACTATAAAGGGCCTGAGCTCCTTGTTGATTTGCAAGACTATGACTATTCATTAGACATGTGGAGCCTTGGCTGTATGTTTGCTGGAATGATCTTTCGCAAGGAACAATTCTTTTATGGTCATGACAATCAGGACCAGCTAGTGAAAATCGCTAAGGCCATCGATTTTCTAGACAAGCTTCTTCGATATGATCATCAGGACAGGCTTACTGCAAGAGAAGCAATGTGGGCGTTCAATGTTATAAAAATTACAAGTTTTGCTAATTCCATGTTTCAGGCACATCCATACTTCTCAGAAGTGAGAAGCGCAGAAATTAGCCGGATTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009791 GO:0010229 GO:0022414 GO:0032501 GO:0032502 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0090567

KEGG Pathways

Metabolic & Signaling

ko03008 ko04064 ko04137 ko04139 ko04310 ko04520 ko04712 ko05162 ko05168 ko05169 map03008 map04064 map04137 map04139 map04310 map04520 map04712 map05162 map05168 map05169

Pfam Domains

Protein Families

Pkinase

Protein Analysis

402

Amino Acids

46.96

Weight (kDa)

9.43

Isoelectric Point (pI)

31.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	137 - 368	4.2e-55	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	139 - 343	2e-18	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002707)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g33300 FvH4_2g33300 FvH4_2g33300 FvH4_2g33300 FvH4_2g33300
malus_domestica	MD08G1087400.v1.1 MD15G1072400.v1.1 MD15G1314700.v1.1
prunus_persica	Prupe.1G425000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0357221 RchiOBHm_Chr1g0362391 RchiOBHm_Chr2g0131551 RchiOBHm_Chr6g0309321
rosa_laevigata	RLG00000009840 RLG00000010574 RLG00000021364 RLG00000031506
rosa_multiflora	Rmu_sc0003576.1_g000007 Rmu_sc0015692.1_g000002
rosa_roxburghii	Rroxscaffold_3G00228160 Rroxscaffold_6G00412270 Rroxscaffold_7G00159490
rosa_rugosa	Rorug01G0255000 Rorug05G0444500 Rorug05G0444500 Rorug06G0371000
rosa_samantha	Rh2BG357900 Rh5AG062100 Rh5AG134700 Rh5BG310400 Rh5DG320900 Rh6AG235600 Rh6AG484400 Rh6BG493500 Rh6CG498300 Rh6DG484800
rosa_wichuraiana	Rw6G028820 Rw6G042170 Rw7G020100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	135, 1121
AatII	GACGTC	1 cut(s)	339
AccI	GTMKAC	1 cut(s)	327
AccII	CGCG	1 cut(s)	228
AciI	CCGC	4 cut(s)	24, 226, 228, 799
AclI	AACGTT	2 cut(s)	342, 777
AclWI	GGATC	1 cut(s)	493
AcsI	RAATTY	4 cut(s)	34, 46, 68, 833
AcyI	GRCGYC	1 cut(s)	336
AfaI	GTAC	3 cut(s)	443, 649, 668
AfiI	CCNNNNNNNGG	2 cut(s)	315, 391
AflIII	ACRYGT	2 cut(s)	637, 936
AgsI	TTSAA	9 cut(s)	46, 151, 283, 389, 401, 635, 658, 876, 1114
AjnI	CCWGG	2 cut(s)	741, 844
AluBI	AGCT	7 cut(s)	188, 583, 607, 872, 898, 1018, 1057
AluI	AGCT	7 cut(s)	188, 583, 607, 872, 898, 1018, 1057
Alw21I	GWGCWC	2 cut(s)	609, 900
AlwI	GGATC	1 cut(s)	493
Ama87I	CYCGRG	2 cut(s)	100, 321
AoxI	GGCC	2 cut(s)	890, 1035
ApeKI	GCWGC	2 cut(s)	259, 583
ApoI	RAATTY	4 cut(s)	34, 46, 68, 833
ArsI	GACNNNNNNTTYG	2 cut(s)	366, 398
AspLEI	GCGC	2 cut(s)	351, 1187
AspS9I	GGNCC	3 cut(s)	566, 890, 1012
AsuC2I	CCSGG	1 cut(s)	163
AsuHPI	GGTGA	1 cut(s)	463
AvaI	CYCGRG	2 cut(s)	100, 321
AvaII	GGWCC	2 cut(s)	566, 1012
BanII	GRGCYC	3 cut(s)	322, 609, 900
BauI	CACGAG	1 cut(s)	791
BbsI	GAAGAC	1 cut(s)	445
Bbv12I	GWGCWC	2 cut(s)	609, 900
BbvI	GCAGC	2 cut(s)	246, 570
BccI	CCATC	3 cut(s)	271, 849, 1046
BciT130I	CCWGG	2 cut(s)	743, 846
BclI	TGATCA	3 cut(s)	492, 787, 1069
BcnI	CCSGG	1 cut(s)	163
BfaI	CTAG	5 cut(s)	240, 315, 621, 1019, 1049
BfoI	RGCGCY	1 cut(s)	352
BisI	GCNGC	2 cut(s)	260, 584
BlsI	GCNGC	2 cut(s)	261, 585
Bme1390I	CCNGG	3 cut(s)	163, 743, 846
Bme18I	GGWCC	2 cut(s)	566, 1012
BmeT110I	CYCGRG	2 cut(s)	100, 321
BmgT120I	GGNCC	3 cut(s)	566, 890, 1012
BmiI	GGNNCC	1 cut(s)	944
BmrFI	CCNGG	3 cut(s)	163, 743, 846
BmrI	ACTGGG	1 cut(s)	373
BmsI	GCATC	2 cut(s)	114, 763
BmuI	ACTGGG	1 cut(s)	373
BpiI	GAAGAC	1 cut(s)	445
Bpu10I	CCTNAGC	2 cut(s)	894, 1032
BpuEI	CTTGAG	1 cut(s)	701
BpuMI	CCSGG	1 cut(s)	163
Bsa29I	ATCGAT	1 cut(s)	1041
BsaBI	GATNNNNATC	1 cut(s)	497
BsaHI	GRCGYC	1 cut(s)	336
BsaJI	CCNNGG	5 cut(s)	78, 226, 741, 742, 946
BsaWI	WCCGGW	2 cut(s)	272, 301
Bsc4I	CCNNNNNNNGG	2 cut(s)	315, 391
Bse1I	ACTGG	2 cut(s)	368, 509
Bse3DI	GCAATG	1 cut(s)	1107
Bse8I	GATNNNNATC	1 cut(s)	497
BseBI	CCWGG	2 cut(s)	743, 846
BseCI	ATCGAT	1 cut(s)	1041
BseDI	CCNNGG	5 cut(s)	78, 226, 741, 742, 946
BseGI	GGATG	4 cut(s)	263, 607, 841, 1159
BseJI	GATNNNNATC	1 cut(s)	497
BseLI	CCNNNNNNNGG	2 cut(s)	315, 391
BseMI	GCAATG	1 cut(s)	1107
BseMII	CTCAG	2 cut(s)	885, 1185
BseNI	ACTGG	2 cut(s)	368, 509
BseXI	GCAGC	2 cut(s)	246, 570
Bsh1236I	CGCG	1 cut(s)	228
BshFI	GGCC	2 cut(s)	892, 1037
BshVI	ATCGAT	1 cut(s)	1041
BsiHKAI	GWGCWC	2 cut(s)	609, 900
BsiHKCI	CYCGRG	2 cut(s)	100, 321
BsiSI	CCGG	4 cut(s)	162, 273, 302, 1198
BslI	CCNNNNNNNGG	2 cut(s)	315, 391
BsnI	GGCC	2 cut(s)	892, 1037
BsoBI	CYCGRG	2 cut(s)	100, 321
Bsp1286I	GDGCHC	3 cut(s)	322, 609, 900
Bsp143I	GATC	5 cut(s)	492, 498, 787, 969, 1069
BspACI	CCGC	4 cut(s)	24, 226, 228, 799
BspANI	GGCC	2 cut(s)	892, 1037
BspCNI	CTCAG	2 cut(s)	886, 1184
BspDI	ATCGAT	1 cut(s)	1041
BspFNI	CGCG	1 cut(s)	228
BspHI	TCATGA	1 cut(s)	1000
BspLI	GGNNCC	1 cut(s)	944
BspPI	GGATC	1 cut(s)	493
BsrDI	GCAATG	1 cut(s)	1107
BsrI	ACTGG	2 cut(s)	368, 509
BssECI	CCNNGG	5 cut(s)	78, 226, 741, 742, 946
BssMI	GATC	5 cut(s)	492, 498, 787, 969, 1069
BssNI	GRCGYC	1 cut(s)	336
BssSI	CACGAG	1 cut(s)	791
BssT1I	CCWWGG	1 cut(s)	946
Bst2BI	CACGAG	1 cut(s)	791
Bst2UI	CCWGG	2 cut(s)	743, 846
Bst4CI	ACNGT	3 cut(s)	446, 647, 734
Bst6I	CTCTTC	1 cut(s)	427
BstACI	GRCGYC	1 cut(s)	336
BstDEI	CTNAG	3 cut(s)	894, 1032, 1171
BstDSI	CCRYGG	1 cut(s)	226
BstF5I	GGATG	4 cut(s)	263, 607, 841, 1159
BstFNI	CGCG	1 cut(s)	228
BstH2I	RGCGCY	1 cut(s)	352
BstHHI	GCGC	2 cut(s)	351, 1187
BstKTI	GATC	5 cut(s)	495, 501, 790, 972, 1072
BstMBI	GATC	5 cut(s)	492, 498, 787, 969, 1069
BstMWI	GCNNNNNNNGC	2 cut(s)	1097, 1106
BstNI	CCWGG	2 cut(s)	743, 846
BstNSI	RCATGY	2 cut(s)	641, 940
BstSCI	CCNGG	3 cut(s)	161, 741, 844
BstUI	CGCG	1 cut(s)	228
BstV1I	GCAGC	2 cut(s)	246, 570
BstV2I	GAAGAC	1 cut(s)	445
BstX2I	RGATCY	1 cut(s)	498
BstYI	RGATCY	1 cut(s)	498
Bsu15I	ATCGAT	1 cut(s)	1041
BsuRI	GGCC	2 cut(s)	892, 1037
BsuTUI	ATCGAT	1 cut(s)	1041
BtgI	CCRYGG	1 cut(s)	226
BtsCI	GGATG	4 cut(s)	263, 607, 841, 1159
BtsIMutI	CAGTG	2 cut(s)	451, 674
CciI	TCATGA	1 cut(s)	1000
CfoI	GCGC	2 cut(s)	351, 1187
Cfr13I	GGNCC	3 cut(s)	566, 890, 1012
Cfr42I	CCGCGG	1 cut(s)	229
ClaI	ATCGAT	1 cut(s)	1041
Csp6I	GTAC	3 cut(s)	442, 648, 667
CviAII	CATG	6 cut(s)	6, 310, 638, 937, 1001, 1147
CviQI	GTAC	3 cut(s)	442, 648, 667
DdeI	CTNAG	3 cut(s)	894, 1032, 1171
DpnI	GATC	5 cut(s)	494, 500, 789, 971, 1071
DpnII	GATC	5 cut(s)	492, 498, 787, 969, 1069
Eam1104I	CTCTTC	1 cut(s)	427
EarI	CTCTTC	1 cut(s)	427
Ecl136II	GAGCTC	2 cut(s)	607, 898
Eco130I	CCWWGG	1 cut(s)	946
Eco24I	GRGCYC	3 cut(s)	322, 609, 900
Eco47I	GGWCC	2 cut(s)	566, 1012
Eco53kI	GAGCTC	2 cut(s)	607, 898
Eco88I	CYCGRG	2 cut(s)	100, 321
EcoICRI	GAGCTC	2 cut(s)	607, 898
EcoO109I	RGGNCCY	1 cut(s)	890
EcoRI	GAATTC	1 cut(s)	833
EcoRII	CCWGG	2 cut(s)	741, 844
EcoT14I	CCWWGG	1 cut(s)	946
EcoT22I	ATGCAT	1 cut(s)	756
EcoT38I	GRGCYC	3 cut(s)	322, 609, 900
ErhI	CCWWGG	1 cut(s)	946
FaeI	CATG	6 cut(s)	9, 313, 641, 940, 1004, 1150
FatI	CATG	6 cut(s)	5, 309, 637, 936, 1000, 1146
FbaI	TGATCA	3 cut(s)	492, 787, 1069
FblI	GTMKAC	1 cut(s)	327
Fnu4HI	GCNGC	2 cut(s)	260, 584
FokI	GGATG	4 cut(s)	250, 614, 828, 1146
FriOI	GRGCYC	3 cut(s)	322, 609, 900
Fsp4HI	GCNGC	2 cut(s)	260, 584
FspBI	CTAG	5 cut(s)	240, 315, 621, 1019, 1049
GlaI	GCGC	2 cut(s)	350, 1186
GluI	GCNGC	2 cut(s)	260, 584
HaeII	RGCGCY	1 cut(s)	352
HaeIII	GGCC	2 cut(s)	892, 1037
HapII	CCGG	4 cut(s)	162, 273, 302, 1198
HhaI	GCGC	2 cut(s)	351, 1187
Hin1I	GRCGYC	1 cut(s)	336
Hin1II	CATG	6 cut(s)	9, 313, 641, 940, 1004, 1150
Hin6I	GCGC	2 cut(s)	349, 1185
HinP1I	GCGC	2 cut(s)	349, 1185
HincII	GTYRAC	2 cut(s)	340, 643
HindII	GTYRAC	2 cut(s)	340, 643
HindIII	AAGCTT	1 cut(s)	1055
HinfI	GANTC	8 cut(s)	141, 158, 276, 287, 324, 374, 553, 1201
HpaII	CCGG	4 cut(s)	162, 273, 302, 1198
HphI	GGTGA	1 cut(s)	463
Hpy166II	GTNNAC	3 cut(s)	328, 340, 643
Hpy188I	TCNGA	5 cut(s)	90, 110, 423, 866, 1174
Hpy188III	TCNNGA	9 cut(s)	401, 496, 514, 758, 791, 1001, 1010, 1049, 1076
Hpy8I	GTNNAC	3 cut(s)	328, 340, 643
Hpy99I	CGWCG	1 cut(s)	338
HpyAV	CCTTC	3 cut(s)	131, 395, 418
HpyCH4III	ACNGT	3 cut(s)	446, 647, 734
HpyCH4IV	ACGT	4 cut(s)	248, 336, 342, 777
HpyCH4V	TGCA	4 cut(s)	550, 754, 913, 1091
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1097, 1106
HpyF3I	CTNAG	3 cut(s)	894, 1032, 1171
HpySE526I	ACGT	4 cut(s)	248, 336, 342, 777
Hsp92I	GRCGYC	1 cut(s)	336
Hsp92II	CATG	6 cut(s)	9, 313, 641, 940, 1004, 1150
HspAI	GCGC	2 cut(s)	349, 1185
Ksp22I	TGATCA	3 cut(s)	492, 787, 1069
KspI	CCGCGG	1 cut(s)	229
Kzo9I	GATC	5 cut(s)	492, 498, 787, 969, 1069
LmnI	GCTCC	2 cut(s)	903, 942
Lsp1109I	GCAGC	2 cut(s)	246, 570
LweI	GCATC	2 cut(s)	114, 763
MaeI	CTAG	5 cut(s)	240, 315, 621, 1019, 1049
MaeII	ACGT	4 cut(s)	248, 336, 342, 777
MaeIII	GTNAC	1 cut(s)	469
MalI	GATC	5 cut(s)	494, 500, 789, 971, 1071
MboI	GATC	5 cut(s)	492, 498, 787, 969, 1069
MboII	GAAGA	5 cut(s)	271, 444, 445, 535, 1052
MflI	RGATCY	1 cut(s)	498
MhlI	GDGCHC	3 cut(s)	322, 609, 900
MlyI	GAGTC	2 cut(s)	150, 333
MmeI	TCCRAC	1 cut(s)	409
MnlI	CCTC	9 cut(s)	88, 135, 277, 406, 428, 442, 451, 557, 780
Mph1103I	ATGCAT	1 cut(s)	756
MseI	TTAA	7 cut(s)	38, 72, 195, 216, 504, 528, 626
MslI	CAYNNNNRTG	1 cut(s)	759
MspA1I	CMGCKG	1 cut(s)	228
MspI	CCGG	4 cut(s)	162, 273, 302, 1198
MspR9I	CCNGG	3 cut(s)	163, 743, 846
MvaI	CCWGG	2 cut(s)	743, 846
MvnI	CGCG	1 cut(s)	228
MwoI	GCNNNNNNNGC	2 cut(s)	1097, 1106
NciI	CCSGG	1 cut(s)	163
NdeII	GATC	5 cut(s)	492, 498, 787, 969, 1069
NlaIII	CATG	6 cut(s)	9, 313, 641, 940, 1004, 1150
NlaIV	GGNNCC	1 cut(s)	944
NmuCI	GTSAC	1 cut(s)	469
NsiI	ATGCAT	1 cut(s)	756
NspI	RCATGY	2 cut(s)	641, 940
PaeR7I	CTCGAG	2 cut(s)	100, 321
PagI	TCATGA	1 cut(s)	1000
PasI	CCCWGGG	1 cut(s)	742
PciI	ACATGT	2 cut(s)	637, 936
PcsI	WCGNNNNNNNCGW	1 cut(s)	378
PfeI	GAWTC	6 cut(s)	158, 276, 287, 374, 553, 1201
PfoI	TCCNGGA	1 cut(s)	161
PkrI	GCNGC	2 cut(s)	261, 585
PleI	GAGTC	2 cut(s)	149, 332
PpsI	GAGTC	2 cut(s)	149, 332
PscI	ACATGT	2 cut(s)	637, 936
PsiI	TTATAA	2 cut(s)	135, 1121
Psp124BI	GAGCTC	2 cut(s)	609, 900
Psp1406I	AACGTT	2 cut(s)	342, 777
Psp6I	CCWGG	2 cut(s)	741, 844
PspGI	CCWGG	2 cut(s)	741, 844
PspN4I	GGNNCC	1 cut(s)	944
PspPI	GGNCC	3 cut(s)	566, 890, 1012
PspXI	VCTCGAGB	2 cut(s)	100, 321
PsuI	RGATCY	1 cut(s)	498
RsaI	GTAC	3 cut(s)	443, 649, 668
RsaNI	GTAC	3 cut(s)	442, 648, 667
RseI	CAYNNNNRTG	1 cut(s)	759
SacI	GAGCTC	2 cut(s)	609, 900
SacII	CCGCGG	1 cut(s)	229
SaqAI	TTAA	7 cut(s)	38, 72, 195, 216, 504, 528, 626
SatI	GCNGC	2 cut(s)	260, 584
Sau3AI	GATC	5 cut(s)	492, 498, 787, 969, 1069
Sau96I	GGNCC	3 cut(s)	566, 890, 1012
SchI	GAGTC	2 cut(s)	150, 333
ScrFI	CCNGG	3 cut(s)	163, 743, 846
SduI	GDGCHC	3 cut(s)	322, 609, 900
SfaNI	GCATC	2 cut(s)	114, 763
Sfr274I	CTCGAG	2 cut(s)	100, 321
Sfr303I	CCGCGG	1 cut(s)	229
SgrBI	CCGCGG	1 cut(s)	229
SinI	GGWCC	2 cut(s)	566, 1012
SlaI	CTCGAG	2 cut(s)	100, 321
SmiMI	CAYNNNNRTG	1 cut(s)	759
SmlI	CTYRAG	3 cut(s)	100, 321, 716
SmoI	CTYRAG	3 cut(s)	100, 321, 716
SsiI	CCGC	4 cut(s)	24, 226, 228, 799
SspI	AATATT	2 cut(s)	546, 574
SspMI	CTAG	5 cut(s)	240, 315, 621, 1019, 1049
SstI	GAGCTC	2 cut(s)	609, 900
StyD4I	CCNGG	3 cut(s)	161, 741, 844
StyI	CCWWGG	1 cut(s)	946
TaaI	ACNGT	3 cut(s)	446, 647, 734
TaiI	ACGT	4 cut(s)	251, 339, 345, 780
TaqI	TCGA	7 cut(s)	55, 101, 322, 456, 757, 1041, 1063
TatI	WGTACW	2 cut(s)	441, 647
TfiI	GAWTC	6 cut(s)	158, 276, 287, 374, 553, 1201
Tru1I	TTAA	7 cut(s)	38, 72, 195, 216, 504, 528, 626
Tru9I	TTAA	7 cut(s)	38, 72, 195, 216, 504, 528, 626
TscAI	CASTG	2 cut(s)	451, 681
TseFI	GTSAC	1 cut(s)	469
TseI	GCWGC	2 cut(s)	259, 583
Tsp45I	GTSAC	1 cut(s)	469
TspDTI	ATGAA	5 cut(s)	81, 420, 495, 723, 918
TspGWI	ACGGA	1 cut(s)	134
TspRI	CASTG	2 cut(s)	451, 681
VpaK11BI	GGWCC	2 cut(s)	566, 1012
XapI	RAATTY	4 cut(s)	34, 46, 68, 833
XbaI	TCTAGA	1 cut(s)	1048
XceI	RCATGY	2 cut(s)	641, 940
XhoI	CTCGAG	2 cut(s)	100, 321
XmiI	GTMKAC	1 cut(s)	327
XspI	CTAG	5 cut(s)	240, 315, 621, 1019, 1049
ZraI	GACGTC	1 cut(s)	337
Zsp2I	ATGCAT	1 cut(s)	756

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.