MD08G1145100.v1.1

Cytochrome C oxidase, subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004102
Physical Location & Seq
Forward (+)
14418061 .. 14420228
2168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1145100.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTGGAGGCGCGTGGCATCTACTTCCTCTGCCCAGCTCCAAAACATCGCCGCCTCAGCCTCACGCAGATCCTCGTCTCTCAATCACACCCGTAGGTTCGTAATCTCCTCGGCGTCTTCCCCTTCGTCTACGCCGTTGCCCAAGTCCGCTCCTCCCGCCGCTCCCCACATTGCCTCCGCTTCCACCTCCCCTCTGAAGAAAAACCCTCAGGAAGCTAATCCGACTGCTACCGGTCACGAGCTTGAGGAACTTGCTGCCGAGCTTGAGGGAAGGGACGTTCTTGAAATCAATTATCCTGTTGGTCCTTTCGGTACCAAGGAAGCACCTGCGATCGTCAAGTCTGTCTATGACAAAAGAATAGTCGGATGCCCTGGTATTGAGGGAGGAGATGATGAGCATGATGTCGTGTGGTTTTGGCTGGAGAAAGGAAAGCCATACGAATGCCCCGTCTGTACCCAAGTTTTTAAGCTGCAAGTGGTTGGCAAAGGAGGTCCTCCAGATGGTCACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005751 GO:0006091 GO:0006119 GO:0006123 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006839 GO:0008150 GO:0008152 GO:0009058 GO:0009060 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0015672 GO:0015980 GO:0015985 GO:0015986 GO:0016020 GO:0016310 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019646 GO:0019693 GO:0019866 GO:0022900 GO:0022904 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0034220 GO:0034641 GO:0034654 GO:0042773 GO:0042775 GO:0042776 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045277 GO:0045333 GO:0046034 GO:0046390 GO:0046483 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055086 GO:0055114 GO:0070069 GO:0070469 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990542
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04260 ko04714 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04260 map04714 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
COX5B

Protein Analysis

171

Amino Acids

18.18

Weight (kDa)

7.01

Isoelectric Point (pI)

66.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX5B PF01215 75 - 164 4.4e-18 Cytochrome c oxidase subunit Vb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 334
Acc65I GGTACC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 2 cut(s) 149, 161
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 12
AciI CCGC 5 cut(s) 51, 147, 156, 159, 177
AclWI GGATC 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 215
AcyI GRCGYC 1 cut(s) 113
AfaI GTAC 2 cut(s) 313, 454
AfiI CCNNNNNNNGG 1 cut(s) 500
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 370
AloI GAACNNNNNNTCC 2 cut(s) 261, 293
AluBI AGCT 5 cut(s) 37, 215, 241, 262, 469
AluI AGCT 5 cut(s) 37, 215, 241, 262, 469
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 254, 469
ArsI GACNNNNNNTTYG 4 cut(s) 345, 377, 432, 464
AsiGI ACCGGT 1 cut(s) 230
Asp718I GGTACC 1 cut(s) 311
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 2 cut(s) 302, 491
AsuHPI GGTGA 1 cut(s) 497
AvaII GGWCC 2 cut(s) 302, 491
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 1 cut(s) 311
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 108
BbvCI CCTCAGC 1 cut(s) 55
BbvI GCAGC 2 cut(s) 241, 456
BccI CCATC 1 cut(s) 494
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 4 cut(s) 51, 159, 255, 470
BlsI GCNGC 4 cut(s) 52, 160, 256, 471
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 302, 491
BmgT120I GGNCC 2 cut(s) 302, 491
BmiI GGNNCC 1 cut(s) 313
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 26, 356
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 2 cut(s) 440, 480
Bpu10I CCTNAGC 1 cut(s) 55
BpuEI CTTGAG 2 cut(s) 263, 284
BsaHI GRCGYC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 108, 315, 370
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse118I RCCGGY 1 cut(s) 230
Bse21I CCTNAGG 1 cut(s) 207
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 3 cut(s) 108, 315, 370
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 69, 221
BseRI GAGGAG 3 cut(s) 97, 141, 399
BseXI GCAGC 2 cut(s) 241, 456
BseYI CCCAGC 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 12
Bsh1285I CGRYCG 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 311
BshTI ACCGGT 1 cut(s) 230
BsiEI CGRYCG 1 cut(s) 333
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 81
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 446
Bsp143I GATC 2 cut(s) 68, 330
BspACI CCGC 5 cut(s) 51, 147, 156, 159, 177
BspCNI CTCAG 2 cut(s) 68, 220
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 311
BsrBI CCGCTC 2 cut(s) 149, 161
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 230
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 3 cut(s) 108, 315, 370
BssMI GATC 2 cut(s) 68, 330
BssNI GRCGYC 1 cut(s) 113
BssSI CACGAG 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 372
BstACI GRCGYC 1 cut(s) 113
BstDEI CTNAG 2 cut(s) 55, 207
BstEII GGTNACC 1 cut(s) 503
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 2 cut(s) 71, 333
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 2 cut(s) 68, 330
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 2 cut(s) 56, 155
BstNI CCWGG 1 cut(s) 372
BstPI GGTNACC 1 cut(s) 503
BstSCI CCNGG 1 cut(s) 370
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 241, 456
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
Bsu36I CCTNAGG 1 cut(s) 207
BtgZI GCGATG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 371
BveI ACCTGC 1 cut(s) 334
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 2 cut(s) 302, 491
CseI GACGC 1 cut(s) 102
Csp6I GTAC 2 cut(s) 312, 453
CspAI ACCGGT 1 cut(s) 230
CviAII CATG 1 cut(s) 398
CviJI RGCY 8 cut(s) 37, 59, 215, 241, 262, 418, 433, 469
CviKI_1 RGCY 8 cut(s) 37, 59, 215, 241, 262, 418, 433, 469
CviQI GTAC 2 cut(s) 312, 453
DdeI CTNAG 2 cut(s) 55, 207
DpnI GATC 2 cut(s) 70, 332
DpnII GATC 2 cut(s) 68, 330
Eco130I CCWWGG 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 302, 491
Eco57I CTGAAG 1 cut(s) 215
Eco81I CCTNAGG 1 cut(s) 207
Eco91I GGTNACC 1 cut(s) 503
EcoO109I RGGNCCY 1 cut(s) 491
EcoO65I GGTNACC 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 1 cut(s) 401
FaiI YATR 3 cut(s) 348, 399, 436
FaqI GGGAC 1 cut(s) 287
FatI CATG 1 cut(s) 397
FauI CCCGC 1 cut(s) 163
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 4 cut(s) 51, 159, 255, 470
FokI GGATG 1 cut(s) 378
Fsp4HI GCNGC 4 cut(s) 51, 159, 255, 470
GlaI GCGC 1 cut(s) 11
GluI GCNGC 4 cut(s) 51, 159, 255, 470
GsaI CCCAGC 1 cut(s) 37
GsuI CTGGAG 2 cut(s) 440, 480
HapII CCGG 1 cut(s) 231
HgaI GACGC 1 cut(s) 102
HhaI GCGC 1 cut(s) 12
Hin1I GRCGYC 1 cut(s) 113
Hin1II CATG 1 cut(s) 401
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 3 cut(s) 195, 222, 365
Hpy188III TCNNGA 4 cut(s) 209, 236, 281, 497
Hpy8I GTNNAC 1 cut(s) 129
HpyAV CCTTC 2 cut(s) 132, 264
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 155
HpyF3I CTNAG 2 cut(s) 55, 207
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 113
Hsp92II CATG 1 cut(s) 401
HspAI GCGC 1 cut(s) 10
KpnI GGTACC 1 cut(s) 315
Kzo9I GATC 2 cut(s) 68, 330
LmnI GCTCC 3 cut(s) 42, 154, 166
LpnPI CCDG 8 cut(s) 47, 194, 244, 309, 339, 357, 384, 404
Lsp1109I GCAGC 2 cut(s) 241, 456
LweI GCATC 2 cut(s) 26, 356
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 233, 503
MalI GATC 2 cut(s) 70, 332
MbiI CCGCTC 2 cut(s) 149, 161
MboI GATC 2 cut(s) 68, 330
MboII GAAGA 2 cut(s) 108, 208
MflI RGATCY 1 cut(s) 68
MluCI AATT 1 cut(s) 289
MmeI TCCRAC 2 cut(s) 245, 343
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 1 cut(s) 439
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 372
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 2 cut(s) 56, 155
NdeII GATC 2 cut(s) 68, 330
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 1 cut(s) 313
NmeAIII GCCGAG 2 cut(s) 89, 283
NmuCI GTSAC 2 cut(s) 233, 503
PaqCI CACCTGC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 2 cut(s) 131, 444
PctI GAATGC 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 4 cut(s) 52, 160, 256, 471
Ple19I CGATCG 1 cut(s) 333
PpuMI RGGWCCY 1 cut(s) 491
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 370
PspEI GGTNACC 1 cut(s) 503
PspFI CCCAGC 1 cut(s) 33
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 1 cut(s) 313
PspPI GGNCC 2 cut(s) 302, 491
PspPPI RGGWCCY 1 cut(s) 491
PsuI RGATCY 1 cut(s) 68
PvuI CGATCG 1 cut(s) 333
RsaI GTAC 2 cut(s) 313, 454
RsaNI GTAC 2 cut(s) 312, 453
RseI CAYNNNNRTG 1 cut(s) 439
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 4 cut(s) 51, 159, 255, 470
Sau3AI GATC 2 cut(s) 68, 330
Sau96I GGNCC 2 cut(s) 302, 491
ScrFI CCNGG 1 cut(s) 372
SfaNI GCATC 2 cut(s) 26, 356
SinI GGWCC 2 cut(s) 302, 491
SmiMI CAYNNNNRTG 1 cut(s) 439
SmlI CTYRAG 2 cut(s) 242, 263
SmoI CTYRAG 2 cut(s) 242, 263
Sse9I AATT 1 cut(s) 289
SsiI CCGC 5 cut(s) 51, 147, 156, 159, 177
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 1 cut(s) 315
TaiI ACGT 1 cut(s) 279
TasI AATT 1 cut(s) 289
TauI GCSGC 2 cut(s) 53, 161
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TseFI GTSAC 2 cut(s) 233, 503
TseI GCWGC 2 cut(s) 254, 469
Tsp45I GTSAC 2 cut(s) 233, 503
VpaK11BI GGWCC 2 cut(s) 302, 491
XmiI GTMKAC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.