MD15G1120800.v1.1

Cytochrome C oxidase, subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004102
Physical Location & Seq
Forward (+)
8685641 .. 8687836
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1120800.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTGGAGGCGCGTGGCATCTACTTCCTCGGCTCAGCTCCAAAACCTCGCCGCCTCAGCCTCACGCAGATCCTCGTCTCTCAATCACACCCGTCGGTTCGTAATCCCCTCGGCGTCTTCCCCTTCGTCGACGCCGTTGGCCAAATCCGCGCCTCCCTCTTCCCCCCACATTGCCTCCGCTTCCACCTCCGCTCTCAAGAAAAACCCTCAAGAAGTTCATCCGGCTGCTACCGGTCACGAGCTTGAGGAACTTGCTGCCGAGCTTGAGGGAAGGGACGTTCTTGAAATCAATTATCCGGTTGGTCCCTTCGGTACCAAGGAAGCACCTGCAATCGTCAAGTCTGTCTATGACAAAAGAATAGTTGGATGCCCTGGTATCGAGGGAGGAGAAGATGAGCACGATGTCGTGTGGTTTTGGCTGGAGAAAGGAAAGATATATGAATGCCCTGTCTGTACTCAAGTTTTTAAGCTGGAAGTGGTAGGCAAAGGGGGTCCTCCAGATGGTCATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005746 GO:0005751 GO:0006091 GO:0006119 GO:0006123 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006839 GO:0008150 GO:0008152 GO:0009058 GO:0009060 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0015672 GO:0015980 GO:0015985 GO:0015986 GO:0016020 GO:0016310 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019646 GO:0019693 GO:0019866 GO:0022900 GO:0022904 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0034220 GO:0034641 GO:0034654 GO:0042773 GO:0042775 GO:0042776 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045277 GO:0045333 GO:0046034 GO:0046390 GO:0046483 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055086 GO:0055114 GO:0070069 GO:0070469 GO:0071704 GO:0072521 GO:0072522 GO:0090407 GO:0098655 GO:0098660 GO:0098662 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600 GO:1990542
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04260 ko04714 ko04932 ko05010 ko05012 ko05016 map00190 map01100 map04260 map04714 map04932 map05010 map05012 map05016
Pfam Domains
Protein Families
COX5B

Protein Analysis

171

Amino Acids

18.22

Weight (kDa)

6.65

Isoelectric Point (pI)

62.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COX5B PF01215 75 - 164 8.9e-19 Cytochrome c oxidase subunit Vb
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 334
Acc65I GGTACC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 1 cut(s) 191
AccI GTMKAC 1 cut(s) 128
AccII CGCG 2 cut(s) 12, 149
AciI CCGC 4 cut(s) 51, 147, 177, 189
AclWI GGATC 1 cut(s) 63
AcoI YGGCCR 1 cut(s) 138
AcyI GRCGYC 2 cut(s) 113, 131
AfaI GTAC 2 cut(s) 313, 454
AfiI CCNNNNNNNGG 1 cut(s) 500
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 1 cut(s) 284
AjnI CCWGG 1 cut(s) 370
AloI GAACNNNNNNTCC 2 cut(s) 261, 293
AluBI AGCT 4 cut(s) 37, 241, 262, 469
AluI AGCT 4 cut(s) 37, 241, 262, 469
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 81
AlwI GGATC 1 cut(s) 63
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 224, 254
AsiGI ACCGGT 1 cut(s) 230
Asp718I GGTACC 1 cut(s) 311
AspLEI GCGC 2 cut(s) 12, 151
AspS9I GGNCC 2 cut(s) 302, 491
AvaII GGWCC 2 cut(s) 302, 491
BalI TGGCCA 1 cut(s) 140
BanI GGYRCC 1 cut(s) 311
BauI CACGAG 1 cut(s) 236
BbsI GAAGAC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 399
BbvCI CCTCAGC 1 cut(s) 55
BbvI GCAGC 2 cut(s) 211, 241
BccI CCATC 1 cut(s) 494
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 372
BcoDI GTCTC 1 cut(s) 81
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 3 cut(s) 51, 225, 255
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 3 cut(s) 52, 226, 256
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 2 cut(s) 302, 491
BmgT120I GGNCC 2 cut(s) 302, 491
BmiI GGNNCC 3 cut(s) 304, 313, 492
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 26, 356
BpiI GAAGAC 1 cut(s) 108
BpmI CTGGAG 2 cut(s) 440, 480
Bpu10I CCTNAGC 1 cut(s) 55
Bpu1102I GCTNAGC 1 cut(s) 33
BpuEI CTTGAG 5 cut(s) 179, 192, 263, 284, 441
BsaBI GATNNNNATC 1 cut(s) 504
BsaHI GRCGYC 2 cut(s) 113, 131
BsaJI CCNNGG 4 cut(s) 27, 108, 315, 370
BsaWI WCCGGW 2 cut(s) 230, 295
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 1 cut(s) 500
Bse118I RCCGGY 1 cut(s) 230
Bse3DI GCAATG 1 cut(s) 168
Bse8I GATNNNNATC 1 cut(s) 504
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 4 cut(s) 27, 108, 315, 370
BseGI GGATG 2 cut(s) 217, 371
BseJI GATNNNNATC 1 cut(s) 504
BseLI CCNNNNNNNGG 1 cut(s) 500
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 47, 69
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 2 cut(s) 211, 241
Bsh1236I CGCG 2 cut(s) 12, 149
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 311
BshTI ACCGGT 1 cut(s) 230
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 221, 231, 296
BslFI GGGAC 2 cut(s) 287, 288
BslI CCNNNNNNNGG 1 cut(s) 500
BsmAI GTCTC 1 cut(s) 81
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 2 cut(s) 287, 288
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 1 cut(s) 68
Bsp1720I GCTNAGC 1 cut(s) 33
BspACI CCGC 4 cut(s) 51, 147, 177, 189
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 2 cut(s) 46, 68
BspFNI CGCG 2 cut(s) 12, 149
BspLI GGNNCC 3 cut(s) 304, 313, 492
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 1 cut(s) 63
BspT107I GGYRCC 1 cut(s) 311
BsrBI CCGCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 1 cut(s) 230
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 4 cut(s) 27, 108, 315, 370
BssMI GATC 1 cut(s) 68
BssNI GRCGYC 2 cut(s) 113, 131
BssSI CACGAG 1 cut(s) 236
BssT1I CCWWGG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 236
Bst2UI CCWGG 1 cut(s) 372
Bst6I CTCTTC 1 cut(s) 163
BstACI GRCGYC 2 cut(s) 113, 131
BstDEI CTNAG 2 cut(s) 33, 55
BstF5I GGATG 2 cut(s) 217, 371
BstFNI CGCG 2 cut(s) 12, 149
BstHHI GCGC 2 cut(s) 12, 151
BstKTI GATC 1 cut(s) 71
BstMAI GTCTC 1 cut(s) 81
BstMBI GATC 1 cut(s) 68
BstMWI GCNNNNNNNGC 2 cut(s) 56, 146
BstNI CCWGG 1 cut(s) 372
BstSCI CCNGG 1 cut(s) 370
BstUI CGCG 2 cut(s) 12, 149
BstV1I GCAGC 2 cut(s) 211, 241
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
BsuRI GGCC 1 cut(s) 140
BtsCI GGATG 2 cut(s) 217, 371
BveI ACCTGC 1 cut(s) 334
CfoI GCGC 2 cut(s) 12, 151
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 2 cut(s) 302, 491
CseI GACGC 2 cut(s) 102, 139
Csp6I GTAC 2 cut(s) 312, 453
CspAI ACCGGT 1 cut(s) 230
CviJI RGCY 9 cut(s) 32, 37, 59, 140, 224, 241, 262, 418, 469
CviKI_1 RGCY 9 cut(s) 32, 37, 59, 140, 224, 241, 262, 418, 469
CviQI GTAC 2 cut(s) 312, 453
DdeI CTNAG 2 cut(s) 33, 55
DpnI GATC 1 cut(s) 70
DpnII GATC 1 cut(s) 68
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 163
EarI CTCTTC 1 cut(s) 163
Eco130I CCWWGG 1 cut(s) 315
Eco47I GGWCC 2 cut(s) 302, 491
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 370
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
Esp3I CGTCTC 1 cut(s) 81
FaiI YATR 3 cut(s) 348, 436, 438
FaqI GGGAC 2 cut(s) 287, 288
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 3 cut(s) 51, 225, 255
FokI GGATG 2 cut(s) 204, 378
Fsp4HI GCNGC 3 cut(s) 51, 225, 255
GlaI GCGC 2 cut(s) 11, 150
GluI GCNGC 3 cut(s) 51, 225, 255
GsuI CTGGAG 2 cut(s) 440, 480
HaeIII GGCC 1 cut(s) 140
HapII CCGG 3 cut(s) 221, 231, 296
HgaI GACGC 2 cut(s) 102, 139
HhaI GCGC 2 cut(s) 12, 151
Hin1I GRCGYC 2 cut(s) 113, 131
Hin6I GCGC 2 cut(s) 10, 149
HinP1I GCGC 2 cut(s) 10, 149
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HpaII CCGG 3 cut(s) 221, 231, 296
Hpy166II GTNNAC 1 cut(s) 129
Hpy188III TCNNGA 5 cut(s) 196, 209, 236, 281, 497
Hpy8I GTNNAC 1 cut(s) 129
Hpy99I CGWCG 3 cut(s) 96, 130, 133
HpyAV CCTTC 3 cut(s) 132, 264, 316
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 146
HpyF3I CTNAG 2 cut(s) 33, 55
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 2 cut(s) 113, 131
HspAI GCGC 2 cut(s) 10, 149
KpnI GGTACC 1 cut(s) 315
Kzo9I GATC 1 cut(s) 68
LmnI GCTCC 1 cut(s) 42
LpnPI CCDG 9 cut(s) 234, 244, 309, 339, 357, 384, 404, 455, 459
Lsp1109I GCAGC 2 cut(s) 211, 241
LweI GCATC 2 cut(s) 26, 356
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 1 cut(s) 233
MalI GATC 1 cut(s) 70
MbiI CCGCTC 1 cut(s) 191
MboI GATC 1 cut(s) 68
MboII GAAGA 3 cut(s) 108, 150, 401
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 1 cut(s) 289
MluNI TGGCCA 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 343
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 140
MspI CCGG 3 cut(s) 221, 231, 296
MspR9I CCNGG 1 cut(s) 372
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 2 cut(s) 12, 149
MwoI GCNNNNNNNGC 2 cut(s) 56, 146
NdeII GATC 1 cut(s) 68
NlaIV GGNNCC 3 cut(s) 304, 313, 492
NmeAIII GCCGAG 3 cut(s) 8, 89, 283
NmuCI GTSAC 1 cut(s) 233
PaqCI CACCTGC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 131
PctI GAATGC 1 cut(s) 446
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 3 cut(s) 52, 226, 256
PpuMI RGGWCCY 1 cut(s) 491
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 370
PspGI CCWGG 1 cut(s) 370
PspN4I GGNNCC 3 cut(s) 304, 313, 492
PspPI GGNCC 2 cut(s) 302, 491
PspPPI RGGWCCY 1 cut(s) 491
PsuI RGATCY 1 cut(s) 68
RsaI GTAC 2 cut(s) 313, 454
RsaNI GTAC 2 cut(s) 312, 453
SalI GTCGAC 1 cut(s) 127
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 3 cut(s) 51, 225, 255
Sau3AI GATC 1 cut(s) 68
Sau96I GGNCC 2 cut(s) 302, 491
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 399
SetI ASST 8 cut(s) 39, 48, 188, 243, 264, 279, 328, 471
SfaNI GCATC 2 cut(s) 26, 356
SgrDI CGTCGACG 1 cut(s) 127
SinI GGWCC 2 cut(s) 302, 491
SmlI CTYRAG 5 cut(s) 194, 207, 242, 263, 456
SmoI CTYRAG 5 cut(s) 194, 207, 242, 263, 456
Sse9I AATT 1 cut(s) 289
SsiI CCGC 4 cut(s) 51, 147, 177, 189
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 1 cut(s) 315
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 128, 378
TasI AATT 1 cut(s) 289
TatI WGTACW 1 cut(s) 452
TauI GCSGC 1 cut(s) 53
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 2 cut(s) 224, 254
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 206, 453
VpaK11BI GGWCC 2 cut(s) 302, 491
XmiI GTMKAC 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.