MD08G1227400.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0004760
Physical Location & Seq
Forward (+)
29377683 .. 29381476
3794 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1227400.v1.1.491

Sequence Viewer

Length: 777 bp
ATGACATCAAGAGACAACCCAACCCCAAACGGAAACGCAACCGCAAACGGAGACCACAGAACCCGCGTCCCACCGCCGCCGTCGTCCTCCTCCTCAAACCCCCACAGCAGCAGCAACCACCACCGCTACTACCCGACGAGGTCGTCATACTCCTCCTCCTCTTCCGCTTCCTTCAAGGGCTGCTGCTGCTGCCTTTTCTTACTCTTCTCCTTCCTAGCCCTCCTCGTCCTCGCCGTCGTCCTCGTCATCATCCTCGCCGTCAAGCCGAAGAAGCCGCAGTTTGATCTCCAGCAAGTCGGCGTCCAGTACATGGGCATCAACTCGCCCAATCCCACCGCAACCGCCGACCCCAACCAAAACCCTACCTCCGCCTCACTCTCCCTCAACATTCGCATGCTCTTCTCCGCTGCTAACCCCAACAAGGTCGGGATCAAGTACGGCGAGTCCAGGTTCACTGTCATGTACCGCGGGATCCCATTGGGCAAGGCCTCCATCCCCGGCTTCTACCAGGACGCCCACACTGTCCGCCAAGTCGTCGCCACCATCGCCGTCGATCGGGTCAATTTGCTCCAAGCCGACGCCGCCGATTTAGTCCGAGACGCTTCCCTCAACGACCGAGTCGAGCTCAGGGTCCTGGGTGACGTTGGCGCCAAGATCCGCGTCTTGAACTTCGATTCCCCTGGCGTTCAGGTGTCCGTGGATTGTGCAATTGTCATAAGTCCAAGGAAGCAGTCTCTCACATACAAGCAGTGTGGATTTGATGGATTGAGTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

259

Amino Acids

27.79

Weight (kDa)

9.41

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 134 - 235 1.6e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 142
AccB1I GGYRCC 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 310
AccII CGCG 3 cut(s) 66, 468, 660
AclWI GGATC 4 cut(s) 437, 466, 479, 649
AcyI GRCGYC 4 cut(s) 300, 513, 579, 648
AfaI GTAC 3 cut(s) 308, 437, 464
AfiI CCNNNNNNNGG 3 cut(s) 310, 421, 555
AgsI TTSAA 2 cut(s) 175, 667
AjnI CCWGG 4 cut(s) 446, 507, 633, 679
AloI GAACNNNNNNTCC 2 cut(s) 659, 691
AluBI AGCT 1 cut(s) 625
AluI AGCT 1 cut(s) 625
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 4 cut(s) 6, 45, 591, 738
AlwI GGATC 4 cut(s) 437, 466, 479, 649
AoxI GGCC 1 cut(s) 486
ApeKI GCWGC 7 cut(s) 108, 111, 180, 183, 186, 189, 407
AspLEI GCGC 1 cut(s) 650
AspS9I GGNCC 1 cut(s) 631
AsuC2I CCSGG 1 cut(s) 498
AsuHPI GGTGA 1 cut(s) 650
AvaII GGWCC 1 cut(s) 631
BamHI GGATCC 1 cut(s) 471
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 7 cut(s) 120, 123, 167, 170, 173, 176, 394
BccI CCATC 3 cut(s) 500, 551, 755
BceAI ACGGC 5 cut(s) 64, 218, 242, 454, 533
BciT130I CCWGG 4 cut(s) 448, 509, 635, 681
BcnI CCSGG 1 cut(s) 498
BcoDI GTCTC 4 cut(s) 6, 45, 591, 738
BfaI CTAG 1 cut(s) 215
BfoI RGCGCY 1 cut(s) 651
Bme1390I CCNGG 5 cut(s) 448, 498, 509, 635, 681
Bme18I GGWCC 1 cut(s) 631
BmgT120I GGNCC 1 cut(s) 631
BmiI GGNNCC 3 cut(s) 473, 632, 649
BmrFI CCNGG 5 cut(s) 448, 498, 509, 635, 681
BmsI GCATC 1 cut(s) 324
BplI GAGNNNNNCTC 2 cut(s) 609, 641
BpmI CTGGAG 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 626
BpuMI CCSGG 1 cut(s) 498
BsaHI GRCGYC 4 cut(s) 300, 513, 579, 648
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 6 cut(s) 466, 496, 634, 679, 696, 722
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 350, 380
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 421, 555
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 4 cut(s) 448, 509, 635, 681
BseDI CCNNGG 6 cut(s) 466, 496, 634, 679, 696, 722
BseGI GGATG 2 cut(s) 249, 492
BseLI CCNNNNNNNGG 3 cut(s) 310, 421, 555
BseMII CTCAG 1 cut(s) 640
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 6 cut(s) 79, 82, 142, 145, 148, 212
BseXI GCAGC 7 cut(s) 120, 123, 167, 170, 173, 176, 394
Bsh1236I CGCG 3 cut(s) 66, 468, 660
Bsh1285I CGRYCG 2 cut(s) 556, 616
BshFI GGCC 1 cut(s) 488
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 2 cut(s) 556, 616
BsiHKAI GWGCWC 1 cut(s) 627
BsiSI CCGG 1 cut(s) 498
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 3 cut(s) 310, 421, 555
BsmAI GTCTC 4 cut(s) 6, 45, 591, 738
BsmBI CGTCTC 1 cut(s) 591
BsmFI GGGAC 1 cut(s) 53
BsnI GGCC 1 cut(s) 488
Bso31I GGTCTC 1 cut(s) 45
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 5 cut(s) 283, 429, 471, 553, 654
BspANI GGCC 1 cut(s) 488
BspCNI CTCAG 1 cut(s) 639
BspFNI CGCG 3 cut(s) 66, 468, 660
BspLI GGNNCC 3 cut(s) 473, 632, 649
BspPI GGATC 4 cut(s) 437, 466, 479, 649
BspQI GCTCTTC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 45
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 6 cut(s) 466, 496, 634, 679, 696, 722
BssMI GATC 5 cut(s) 283, 429, 471, 553, 654
BssNI GRCGYC 4 cut(s) 300, 513, 579, 648
BssT1I CCWWGG 1 cut(s) 722
Bst2UI CCWGG 4 cut(s) 448, 509, 635, 681
Bst4CI ACNGT 2 cut(s) 457, 523
Bst6I CTCTTC 3 cut(s) 166, 209, 404
BstACI GRCGYC 4 cut(s) 300, 513, 579, 648
BstC8I GCNNGC 1 cut(s) 395
BstDEI CTNAG 1 cut(s) 626
BstDSI CCRYGG 2 cut(s) 466, 696
BstF5I GGATG 2 cut(s) 249, 492
BstFNI CGCG 3 cut(s) 66, 468, 660
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 1 cut(s) 650
BstKTI GATC 5 cut(s) 286, 432, 474, 556, 657
BstMAI GTCTC 4 cut(s) 6, 45, 591, 738
BstMBI GATC 5 cut(s) 283, 429, 471, 553, 654
BstMCI CGRYCG 2 cut(s) 556, 616
BstMWI GCNNNNNNNGC 5 cut(s) 186, 189, 271, 545, 581
BstNI CCWGG 4 cut(s) 448, 509, 635, 681
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 5 cut(s) 446, 496, 507, 633, 679
BstUI CGCG 3 cut(s) 66, 468, 660
BstV1I GCAGC 7 cut(s) 120, 123, 167, 170, 173, 176, 394
BstX2I RGATCY 2 cut(s) 471, 654
BstYI RGATCY 2 cut(s) 471, 654
BsuRI GGCC 1 cut(s) 488
BtgI CCRYGG 2 cut(s) 466, 696
BtgZI GCGATG 1 cut(s) 529
BtsCI GGATG 2 cut(s) 249, 492
BtsI GCAGTG 1 cut(s) 755
BtsIMutI CAGTG 3 cut(s) 453, 519, 755
Cac8I GCNNGC 1 cut(s) 395
CfoI GCGC 1 cut(s) 650
Cfr13I GGNCC 1 cut(s) 631
Cfr42I CCGCGG 1 cut(s) 469
CseI GACGC 6 cut(s) 55, 289, 521, 587, 608, 649
Csp6I GTAC 3 cut(s) 307, 436, 463
CspCI CAANNNNNGTGG 2 cut(s) 733, 768
CviAII CATG 3 cut(s) 310, 394, 460
CviJI RGCY 8 cut(s) 180, 218, 265, 274, 488, 501, 575, 625
CviKI_1 RGCY 8 cut(s) 180, 218, 265, 274, 488, 501, 575, 625
CviQI GTAC 3 cut(s) 307, 436, 463
DdeI CTNAG 1 cut(s) 626
DinI GGCGCC 1 cut(s) 649
DpnI GATC 5 cut(s) 285, 431, 473, 555, 656
DpnII GATC 5 cut(s) 283, 429, 471, 553, 654
DrdI GACNNNNNNGTC 1 cut(s) 142
DseDI GACNNNNNNGTC 1 cut(s) 142
Eam1104I CTCTTC 3 cut(s) 166, 209, 404
EarI CTCTTC 3 cut(s) 166, 209, 404
EciI GGCGGA 2 cut(s) 358, 515
Ecl136II GAGCTC 1 cut(s) 625
Eco130I CCWWGG 1 cut(s) 722
Eco147I AGGCCT 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 625
EcoICRI GAGCTC 1 cut(s) 625
EcoO109I RGGNCCY 1 cut(s) 631
EcoRII CCWGG 4 cut(s) 446, 507, 633, 679
EcoT14I CCWWGG 1 cut(s) 722
EcoT38I GRGCYC 1 cut(s) 627
EgeI GGCGCC 1 cut(s) 649
EheI GGCGCC 1 cut(s) 649
ErhI CCWWGG 1 cut(s) 722
Esp3I CGTCTC 1 cut(s) 591
FaeI CATG 3 cut(s) 313, 397, 463
FaiI YATR 6 cut(s) 148, 311, 395, 461, 716, 742
FaqI GGGAC 1 cut(s) 53
FatI CATG 3 cut(s) 309, 393, 459
FauI CCCGC 2 cut(s) 71, 461
FokI GGATG 2 cut(s) 236, 479
FriOI GRGCYC 1 cut(s) 627
FspBI CTAG 1 cut(s) 215
GlaI GCGC 1 cut(s) 649
GsuI CTGGAG 1 cut(s) 272
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 1 cut(s) 488
HapII CCGG 1 cut(s) 498
HgaI GACGC 6 cut(s) 55, 289, 521, 587, 608, 649
HhaI GCGC 1 cut(s) 650
Hin1I GRCGYC 4 cut(s) 300, 513, 579, 648
Hin1II CATG 3 cut(s) 313, 397, 463
Hin6I GCGC 1 cut(s) 648
HinP1I GCGC 1 cut(s) 648
HinfI GANTC 3 cut(s) 443, 618, 674
HpaII CCGG 1 cut(s) 498
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 1 cut(s) 596
Hpy188III TCNNGA 3 cut(s) 9, 427, 664
Hpy8I GTNNAC 1 cut(s) 453
Hpy99I CGWCG 6 cut(s) 85, 139, 239, 539, 554, 581
HpyAV CCTTC 2 cut(s) 181, 220
HpyCH4III ACNGT 2 cut(s) 457, 523
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 1 cut(s) 707
HpyF10VI GCNNNNNNNGC 5 cut(s) 186, 189, 271, 545, 581
HpyF3I CTNAG 1 cut(s) 626
HpySE526I ACGT 1 cut(s) 642
Hsp92I GRCGYC 4 cut(s) 300, 513, 579, 648
Hsp92II CATG 3 cut(s) 313, 397, 463
HspAI GCGC 1 cut(s) 648
KasI GGCGCC 1 cut(s) 647
KspI CCGCGG 1 cut(s) 469
Kzo9I GATC 5 cut(s) 283, 429, 471, 553, 654
LguI GCTCTTC 1 cut(s) 404
LmnI GCTCC 1 cut(s) 573
Lsp1109I GCAGC 7 cut(s) 120, 123, 167, 170, 173, 176, 394
LweI GCATC 1 cut(s) 324
MaeI CTAG 1 cut(s) 215
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 1 cut(s) 638
MalI GATC 5 cut(s) 285, 431, 473, 555, 656
MboI GATC 5 cut(s) 283, 429, 471, 553, 654
MboII GAAGA 4 cut(s) 153, 196, 280, 391
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 2 cut(s) 471, 654
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 2 cut(s) 562, 708
Mly113I GGCGCC 1 cut(s) 648
MlyI GAGTC 2 cut(s) 452, 627
MslI CAYNNNNRTG 2 cut(s) 392, 458
MspA1I CMGCKG 2 cut(s) 407, 468
MspI CCGG 1 cut(s) 498
MspR9I CCNGG 5 cut(s) 448, 498, 509, 635, 681
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 4 cut(s) 448, 509, 635, 681
MvnI CGCG 3 cut(s) 66, 468, 660
MwoI GCNNNNNNNGC 5 cut(s) 186, 189, 271, 545, 581
NarI GGCGCC 1 cut(s) 648
NciI CCSGG 1 cut(s) 498
NdeII GATC 5 cut(s) 283, 429, 471, 553, 654
NlaIII CATG 3 cut(s) 313, 397, 463
NlaIV GGNNCC 3 cut(s) 473, 632, 649
NmuCI GTSAC 1 cut(s) 638
NspI RCATGY 1 cut(s) 397
PaeI GCATGC 1 cut(s) 397
PceI AGGCCT 1 cut(s) 488
PciSI GCTCTTC 1 cut(s) 404
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 618
PfeI GAWTC 1 cut(s) 674
PflFI GACNNNGTC 2 cut(s) 139, 617
PflMI CCANNNNNTGG 1 cut(s) 310
Ple19I CGATCG 1 cut(s) 556
PleI GAGTC 2 cut(s) 451, 626
PluTI GGCGCC 1 cut(s) 651
PpsI GAGTC 2 cut(s) 451, 626
PpuMI RGGWCCY 1 cut(s) 631
Psp124BI GAGCTC 1 cut(s) 627
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 4 cut(s) 446, 507, 633, 679
PspGI CCWGG 4 cut(s) 446, 507, 633, 679
PspN4I GGNNCC 3 cut(s) 473, 632, 649
PspPI GGNCC 1 cut(s) 631
PspPPI RGGWCCY 1 cut(s) 631
PsuI RGATCY 2 cut(s) 471, 654
PsyI GACNNNGTC 2 cut(s) 139, 617
PvuI CGATCG 1 cut(s) 556
RsaI GTAC 3 cut(s) 308, 437, 464
RsaNI GTAC 3 cut(s) 307, 436, 463
RseI CAYNNNNRTG 2 cut(s) 392, 458
SacI GAGCTC 1 cut(s) 627
SacII CCGCGG 1 cut(s) 469
SapI GCTCTTC 1 cut(s) 404
Sau3AI GATC 5 cut(s) 283, 429, 471, 553, 654
Sau96I GGNCC 1 cut(s) 631
SchI GAGTC 2 cut(s) 452, 627
ScrFI CCNGG 5 cut(s) 448, 498, 509, 635, 681
SduI GDGCHC 1 cut(s) 627
SetI ASST 7 cut(s) 143, 368, 426, 452, 627, 645, 693
SfaNI GCATC 1 cut(s) 324
SfoI GGCGCC 1 cut(s) 649
Sfr303I CCGCGG 1 cut(s) 469
SgrBI CCGCGG 1 cut(s) 469
SinI GGWCC 1 cut(s) 631
SmiMI CAYNNNNRTG 2 cut(s) 392, 458
SphI GCATGC 1 cut(s) 397
Sse9I AATT 2 cut(s) 562, 708
SseBI AGGCCT 1 cut(s) 488
SspDI GGCGCC 1 cut(s) 647
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 627
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 5 cut(s) 446, 496, 507, 633, 679
StyI CCWWGG 1 cut(s) 722
TaaI ACNGT 2 cut(s) 457, 523
TaiI ACGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 552, 621, 672
TaqII GACCGA 1 cut(s) 630
TasI AATT 2 cut(s) 562, 708
TatI WGTACW 1 cut(s) 306
TauI GCSGC 3 cut(s) 79, 277, 584
TfiI GAWTC 1 cut(s) 674
TscAI CASTG 3 cut(s) 460, 526, 755
TseFI GTSAC 1 cut(s) 638
TseI GCWGC 7 cut(s) 108, 111, 180, 183, 186, 189, 407
Tsp45I GTSAC 1 cut(s) 638
TspGWI ACGGA 3 cut(s) 45, 63, 685
TspRI CASTG 3 cut(s) 460, 526, 755
Tth111I GACNNNGTC 2 cut(s) 139, 617
Van91I CCANNNNNTGG 1 cut(s) 310
VpaK11BI GGWCC 1 cut(s) 631
XceI RCATGY 1 cut(s) 397
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.