MD15G1419800.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004760
Physical Location & Seq
Forward (+)
52134508 .. 52138104
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1419800.v1.1.491

Sequence Viewer

Length: 804 bp
ATGACATCAACAGCCAACCCAAACCCGAACCGGAACCCAATCCCAAACGGAAACGCAACCGCAAACGGCGACCACAGACCCCGTGCCCCACCGCCACGACAGCCGCCGTCGCCCTCCTCCTCTTCTCACCCCCACAACAGCAGCAACCACCGCCACTACTATCCCACGACCTCCTCATCCTCCTCCTCCTCCGCTTCCTTCAAGGGCTGCTGCTGCTGCCTCTTCTTACTCTTCTCCTTCCTAGCCCTCCTCGTCCTCGCCGTCGTCCTCGTCATCGTCCTCGCCCTCAAGCCGAAGAAGCCGCAGTTCGATCTCCAGCAAGTGGGCGTCCAGTACATGGGCATCAACTCACCCAATCCCACCCCAACCGCCGACCCCAACCAAAACCCTACCTCCGCCTCCCTCTCCCTCAACATTCGCATGCTCTTCTCCGCCGCTAACCCCAACAAGGTCGGGATCAAGTACGGCGAGTCCAGGTTCACTGTCATGTACCGCGGGATCCCATTGGGCAAGGCCTCCATCCCCGGCTTCTACCAGGACGCCCACACTGTCCGCCAAGTCGTCGCCACCATCGCCGTCGATCGGGTCAATTTGCTCCAAGCCGACGCCACCGATTTAGTTCGAGACGCTTCACTCAACGACCGAGTCGAGCTCAGGGTCTTGGGTGACGTTGGCGCCAAGATCCGCGTCTTAAACTTCGATTCCCCAGGCGTTCAGGTGTCGGTGGACTGTGCGATTGTCATAAGTCCAAGGAAGCAGTCTCTTTCATACAAGCAGTGCGGATTTGATGGATTGAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

268

Amino Acids

28.71

Weight (kDa)

9.53

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 143 - 244 2.4e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 2 cut(s) 322, 337
AccII CGCG 2 cut(s) 495, 687
AclWI GGATC 4 cut(s) 464, 493, 506, 676
AcyI GRCGYC 4 cut(s) 327, 540, 606, 675
AfaI GTAC 3 cut(s) 335, 464, 491
AfiI CCNNNNNNNGG 4 cut(s) 322, 337, 448, 582
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 3 cut(s) 473, 534, 706
AluBI AGCT 1 cut(s) 652
AluI AGCT 1 cut(s) 652
Alw21I GWGCWC 1 cut(s) 654
Alw26I GTCTC 2 cut(s) 618, 765
AlwI GGATC 4 cut(s) 464, 493, 506, 676
AoxI GGCC 1 cut(s) 513
ApeKI GCWGC 5 cut(s) 141, 207, 210, 213, 216
AspLEI GCGC 1 cut(s) 677
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 3 cut(s) 119, 342, 677
BaeGI GKGCMC 1 cut(s) 88
BamHI GGATCC 1 cut(s) 498
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 654
Bbv12I GWGCWC 1 cut(s) 654
BbvI GCAGC 5 cut(s) 153, 194, 197, 200, 203
BccI CCATC 3 cut(s) 527, 578, 782
BceAI ACGGC 5 cut(s) 82, 91, 245, 481, 560
BciT130I CCWGG 3 cut(s) 475, 536, 708
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 618, 765
BfaI CTAG 1 cut(s) 242
BfoI RGCGCY 1 cut(s) 678
BisI GCNGC 8 cut(s) 104, 142, 208, 211, 214, 217, 302, 435
BlsI GCNGC 8 cut(s) 105, 143, 209, 212, 215, 218, 303, 436
Bme1390I CCNGG 4 cut(s) 475, 525, 536, 708
BmiI GGNNCC 3 cut(s) 35, 500, 676
BmrFI CCNGG 4 cut(s) 475, 525, 536, 708
BmsI GCATC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 636, 668
BpmI CTGGAG 1 cut(s) 299
Bpu10I CCTNAGC 1 cut(s) 653
BpuEI CTTGAG 1 cut(s) 272
BpuMI CCSGG 1 cut(s) 525
BsaHI GRCGYC 4 cut(s) 327, 540, 606, 675
BsaJI CCNNGG 4 cut(s) 493, 523, 706, 749
BsaWI WCCGGW 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 4 cut(s) 322, 337, 448, 582
Bse1I ACTGG 1 cut(s) 331
BseBI CCWGG 3 cut(s) 475, 536, 708
BseDI CCNNGG 4 cut(s) 493, 523, 706, 749
BseGI GGATG 2 cut(s) 176, 519
BseLI CCNNNNNNNGG 4 cut(s) 322, 337, 448, 582
BseMII CTCAG 1 cut(s) 667
BseNI ACTGG 1 cut(s) 331
BseRI GAGGAG 7 cut(s) 106, 109, 163, 172, 175, 178, 239
BseSI GKGCMC 1 cut(s) 88
BseXI GCAGC 5 cut(s) 153, 194, 197, 200, 203
Bsh1236I CGCG 2 cut(s) 495, 687
Bsh1285I CGRYCG 2 cut(s) 583, 643
BshFI GGCC 1 cut(s) 515
BshNI GGYRCC 1 cut(s) 674
BsiEI CGRYCG 2 cut(s) 583, 643
BsiHKAI GWGCWC 1 cut(s) 654
BsiSI CCGG 2 cut(s) 31, 525
BslI CCNNNNNNNGG 4 cut(s) 322, 337, 448, 582
BsmAI GTCTC 2 cut(s) 618, 765
BsmBI CGTCTC 1 cut(s) 618
BsnI GGCC 1 cut(s) 515
Bsp1286I GDGCHC 2 cut(s) 88, 654
Bsp143I GATC 5 cut(s) 310, 456, 498, 580, 681
BspANI GGCC 1 cut(s) 515
BspCNI CTCAG 1 cut(s) 666
BspFNI CGCG 2 cut(s) 495, 687
BspLI GGNNCC 3 cut(s) 35, 500, 676
BspPI GGATC 4 cut(s) 464, 493, 506, 676
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 674
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 4 cut(s) 493, 523, 706, 749
BssMI GATC 5 cut(s) 310, 456, 498, 580, 681
BssNI GRCGYC 4 cut(s) 327, 540, 606, 675
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 3 cut(s) 475, 536, 708
Bst4CI ACNGT 3 cut(s) 484, 550, 731
Bst6I CTCTTC 4 cut(s) 127, 227, 236, 431
BstACI GRCGYC 4 cut(s) 327, 540, 606, 675
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 653
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 2 cut(s) 176, 519
BstFNI CGCG 2 cut(s) 495, 687
BstH2I RGCGCY 1 cut(s) 678
BstHHI GCGC 1 cut(s) 677
BstKTI GATC 5 cut(s) 313, 459, 501, 583, 684
BstMAI GTCTC 2 cut(s) 618, 765
BstMBI GATC 5 cut(s) 310, 456, 498, 580, 681
BstMCI CGRYCG 2 cut(s) 583, 643
BstMWI GCNNNNNNNGC 7 cut(s) 100, 109, 150, 213, 216, 298, 572
BstNI CCWGG 3 cut(s) 475, 536, 708
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 473, 523, 534, 706
BstSLI GKGCMC 1 cut(s) 88
BstUI CGCG 2 cut(s) 495, 687
BstV1I GCAGC 5 cut(s) 153, 194, 197, 200, 203
BstX2I RGATCY 2 cut(s) 498, 681
BstYI RGATCY 2 cut(s) 498, 681
BsuRI GGCC 1 cut(s) 515
BtgI CCRYGG 1 cut(s) 493
BtgZI GCGATG 1 cut(s) 556
BtsCI GGATG 2 cut(s) 176, 519
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 3 cut(s) 480, 546, 782
Cac8I GCNNGC 1 cut(s) 422
CfoI GCGC 1 cut(s) 677
Cfr42I CCGCGG 1 cut(s) 496
CseI GACGC 5 cut(s) 316, 548, 614, 635, 676
Csp6I GTAC 3 cut(s) 334, 463, 490
CviAII CATG 3 cut(s) 337, 421, 487
CviQI GTAC 3 cut(s) 334, 463, 490
DdeI CTNAG 1 cut(s) 653
DinI GGCGCC 1 cut(s) 676
DpnI GATC 5 cut(s) 312, 458, 500, 582, 683
DpnII GATC 5 cut(s) 310, 456, 498, 580, 681
Eam1104I CTCTTC 4 cut(s) 127, 227, 236, 431
EarI CTCTTC 4 cut(s) 127, 227, 236, 431
EciI GGCGGA 3 cut(s) 385, 421, 542
Ecl136II GAGCTC 1 cut(s) 652
Eco130I CCWWGG 1 cut(s) 749
Eco147I AGGCCT 1 cut(s) 515
Eco24I GRGCYC 1 cut(s) 654
Eco53kI GAGCTC 1 cut(s) 652
EcoICRI GAGCTC 1 cut(s) 652
EcoRII CCWGG 3 cut(s) 473, 534, 706
EcoT14I CCWWGG 1 cut(s) 749
EcoT38I GRGCYC 1 cut(s) 654
EgeI GGCGCC 1 cut(s) 676
EheI GGCGCC 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 749
Esp3I CGTCTC 1 cut(s) 618
FaeI CATG 3 cut(s) 340, 424, 490
FaiI YATR 5 cut(s) 338, 422, 488, 743, 769
FatI CATG 3 cut(s) 336, 420, 486
FauI CCCGC 1 cut(s) 488
Fnu4HI GCNGC 8 cut(s) 104, 142, 208, 211, 214, 217, 302, 435
FokI GGATG 2 cut(s) 163, 506
FriOI GRGCYC 1 cut(s) 654
Fsp4HI GCNGC 8 cut(s) 104, 142, 208, 211, 214, 217, 302, 435
FspBI CTAG 1 cut(s) 242
GlaI GCGC 1 cut(s) 676
GluI GCNGC 8 cut(s) 104, 142, 208, 211, 214, 217, 302, 435
GsuI CTGGAG 1 cut(s) 299
HaeII RGCGCY 1 cut(s) 678
HaeIII GGCC 1 cut(s) 515
HapII CCGG 2 cut(s) 31, 525
HgaI GACGC 5 cut(s) 316, 548, 614, 635, 676
HhaI GCGC 1 cut(s) 677
Hin1I GRCGYC 4 cut(s) 327, 540, 606, 675
Hin1II CATG 3 cut(s) 340, 424, 490
Hin6I GCGC 1 cut(s) 675
HinP1I GCGC 1 cut(s) 675
HinfI GANTC 3 cut(s) 470, 645, 701
HpaII CCGG 2 cut(s) 31, 525
HphI GGTGA 3 cut(s) 119, 342, 677
Hpy166II GTNNAC 2 cut(s) 480, 727
Hpy188III TCNNGA 2 cut(s) 454, 623
Hpy8I GTNNAC 2 cut(s) 480, 727
Hpy99I CGWCG 5 cut(s) 112, 266, 566, 581, 608
HpyAV CCTTC 2 cut(s) 208, 247
HpyCH4III ACNGT 3 cut(s) 484, 550, 731
HpyCH4IV ACGT 1 cut(s) 669
HpyF10VI GCNNNNNNNGC 7 cut(s) 100, 109, 150, 213, 216, 298, 572
HpyF3I CTNAG 1 cut(s) 653
HpySE526I ACGT 1 cut(s) 669
Hsp92I GRCGYC 4 cut(s) 327, 540, 606, 675
Hsp92II CATG 3 cut(s) 340, 424, 490
HspAI GCGC 1 cut(s) 675
KasI GGCGCC 1 cut(s) 674
KspI CCGCGG 1 cut(s) 496
Kzo9I GATC 5 cut(s) 310, 456, 498, 580, 681
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 1 cut(s) 600
Lsp1109I GCAGC 5 cut(s) 153, 194, 197, 200, 203
LweI GCATC 1 cut(s) 351
MaeI CTAG 1 cut(s) 242
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 1 cut(s) 665
MalI GATC 5 cut(s) 312, 458, 500, 582, 683
MboI GATC 5 cut(s) 310, 456, 498, 580, 681
MboII GAAGA 5 cut(s) 114, 214, 223, 307, 418
MflI RGATCY 2 cut(s) 498, 681
MhlI GDGCHC 2 cut(s) 88, 654
MluCI AATT 1 cut(s) 589
Mly113I GGCGCC 1 cut(s) 675
MlyI GAGTC 2 cut(s) 479, 654
MseI TTAA 1 cut(s) 692
MslI CAYNNNNRTG 2 cut(s) 419, 485
MspA1I CMGCKG 1 cut(s) 495
MspI CCGG 2 cut(s) 31, 525
MspR9I CCNGG 4 cut(s) 475, 525, 536, 708
MvaI CCWGG 3 cut(s) 475, 536, 708
MvnI CGCG 2 cut(s) 495, 687
MwoI GCNNNNNNNGC 7 cut(s) 100, 109, 150, 213, 216, 298, 572
NarI GGCGCC 1 cut(s) 675
NciI CCSGG 1 cut(s) 525
NdeII GATC 5 cut(s) 310, 456, 498, 580, 681
NlaIII CATG 3 cut(s) 340, 424, 490
NlaIV GGNNCC 3 cut(s) 35, 500, 676
NmuCI GTSAC 1 cut(s) 665
NspI RCATGY 1 cut(s) 424
PaeI GCATGC 1 cut(s) 424
PceI AGGCCT 1 cut(s) 515
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 645
PfeI GAWTC 1 cut(s) 701
PflFI GACNNNGTC 1 cut(s) 644
PflMI CCANNNNNTGG 2 cut(s) 322, 337
PkrI GCNGC 8 cut(s) 105, 143, 209, 212, 215, 218, 303, 436
Ple19I CGATCG 1 cut(s) 583
PleI GAGTC 2 cut(s) 478, 653
PluTI GGCGCC 1 cut(s) 678
PpsI GAGTC 2 cut(s) 478, 653
Psp124BI GAGCTC 1 cut(s) 654
Psp6I CCWGG 3 cut(s) 473, 534, 706
PspGI CCWGG 3 cut(s) 473, 534, 706
PspN4I GGNNCC 3 cut(s) 35, 500, 676
PsuI RGATCY 2 cut(s) 498, 681
PsyI GACNNNGTC 1 cut(s) 644
PvuI CGATCG 1 cut(s) 583
RsaI GTAC 3 cut(s) 335, 464, 491
RsaNI GTAC 3 cut(s) 334, 463, 490
RseI CAYNNNNRTG 2 cut(s) 419, 485
SacI GAGCTC 1 cut(s) 654
SacII CCGCGG 1 cut(s) 496
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 1 cut(s) 692
SatI GCNGC 8 cut(s) 104, 142, 208, 211, 214, 217, 302, 435
Sau3AI GATC 5 cut(s) 310, 456, 498, 580, 681
SchI GAGTC 2 cut(s) 479, 654
ScrFI CCNGG 4 cut(s) 475, 525, 536, 708
SduI GDGCHC 2 cut(s) 88, 654
SetI ASST 7 cut(s) 173, 395, 453, 479, 654, 672, 720
SfaNI GCATC 1 cut(s) 351
SfoI GGCGCC 1 cut(s) 676
Sfr303I CCGCGG 1 cut(s) 496
SgrBI CCGCGG 1 cut(s) 496
SmiMI CAYNNNNRTG 2 cut(s) 419, 485
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SphI GCATGC 1 cut(s) 424
Sse9I AATT 1 cut(s) 589
SseBI AGGCCT 1 cut(s) 515
SspDI GGCGCC 1 cut(s) 674
SspMI CTAG 1 cut(s) 242
SstI GAGCTC 1 cut(s) 654
StuI AGGCCT 1 cut(s) 515
StyD4I CCNGG 4 cut(s) 473, 523, 534, 706
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 3 cut(s) 484, 550, 731
TaiI ACGT 1 cut(s) 672
TaqI TCGA 5 cut(s) 309, 579, 622, 648, 699
TaqII GACCGA 1 cut(s) 657
TasI AATT 1 cut(s) 589
TatI WGTACW 1 cut(s) 333
TauI GCSGC 3 cut(s) 106, 304, 437
TfiI GAWTC 1 cut(s) 701
Tru1I TTAA 1 cut(s) 692
Tru9I TTAA 1 cut(s) 692
TscAI CASTG 3 cut(s) 487, 553, 782
TseFI GTSAC 1 cut(s) 665
TseI GCWGC 5 cut(s) 141, 207, 210, 213, 216
Tsp45I GTSAC 1 cut(s) 665
TspDTI ATGAA 1 cut(s) 756
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 3 cut(s) 487, 553, 782
Tth111I GACNNNGTC 1 cut(s) 644
Van91I CCANNNNNTGG 2 cut(s) 322, 337
XceI RCATGY 1 cut(s) 424
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.