RchiOBHm_Chr7g0223311

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004760
Physical Location & Seq
Reverse (-)
44998121 .. 45001476
3356 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19995

Sequence Viewer

Length: 459 bp
ATGGGCATCAACTCGCCCAATCCCACCACCGACCCGAACCCGGCCTCCTCCGCCTCTCTCTCCCTCAGCATTCGCCTGCTCTTCTCCGCCGAGAACCCCAACAAGGTCGGAATCAAGTACGGCGAGTCCAGGTTCACTGTCATGTACCGCGGCATCCCTTTGGGCAAGGCCTCCGTCCCCGGCTTCTTCCAGCAGGCCCACAGCGTCAGGCAGGTCGTCGCCACCATCGCCGTCGATCGCGCCAATCTCATTCAGGCCGACGCCGCCGACTTAATCCGCGACGCCTCCCTCAACGACCGGGTCGAGCTCCGGGTCTTGGGTGACGTCGGCGCCAAGATCCGCGTCTTGAACATCGATTCCCCTGGCGTTCAGGTTTCTGTGGATTGTGCAATCGTTATAAGTCCAAGGAAGCAGTCTCTTACTTACAAGCAGTGTGGATTTGATGGATTGAGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

152

Amino Acids

16.23

Weight (kDa)

9.06

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 28 - 127 4.3e-12 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 398
AatII GACGTC 1 cut(s) 327
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 329
AccII CGCG 4 cut(s) 150, 240, 279, 342
AciI CCGC 7 cut(s) 51, 87, 148, 150, 264, 277, 340
AclWI GGATC 1 cut(s) 331
AcyI GRCGYC 4 cut(s) 261, 282, 324, 330
AfaI GTAC 2 cut(s) 119, 146
AfiI CCNNNNNNNGG 3 cut(s) 40, 103, 316
AgsI TTSAA 1 cut(s) 349
AjnI CCWGG 2 cut(s) 128, 361
AloI GAACNNNNNNTCC 4 cut(s) 29, 61, 341, 373
AluBI AGCT 1 cut(s) 307
AluI AGCT 1 cut(s) 307
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 1 cut(s) 420
AlwI GGATC 1 cut(s) 331
AoxI GGCC 4 cut(s) 42, 168, 195, 255
AspLEI GCGC 2 cut(s) 242, 332
AspS9I GGNCC 1 cut(s) 196
AsuC2I CCSGG 4 cut(s) 41, 180, 299, 311
AsuHPI GGTGA 1 cut(s) 332
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 309
Bbv12I GWGCWC 1 cut(s) 309
BbvCI CCTCAGC 1 cut(s) 65
BccI CCATC 2 cut(s) 233, 437
BceAI ACGGC 2 cut(s) 136, 215
BciT130I CCWGG 2 cut(s) 130, 363
BcnI CCSGG 4 cut(s) 41, 180, 299, 311
BcoDI GTCTC 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 202
BisI GCNGC 2 cut(s) 151, 264
BlsI GCNGC 2 cut(s) 152, 265
Bme1390I CCNGG 6 cut(s) 41, 130, 180, 299, 311, 363
BmgT120I GGNCC 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 6 cut(s) 41, 130, 180, 299, 311, 363
BmsI GCATC 2 cut(s) 15, 162
Bpu10I CCTNAGC 1 cut(s) 65
BpuMI CCSGG 4 cut(s) 41, 180, 299, 311
Bsa29I ATCGAT 1 cut(s) 354
BsaHI GRCGYC 4 cut(s) 261, 282, 324, 330
BsaJI CCNNGG 4 cut(s) 148, 178, 361, 404
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 3 cut(s) 40, 103, 316
BseBI CCWGG 2 cut(s) 130, 363
BseCI ATCGAT 1 cut(s) 354
BseDI CCNNGG 4 cut(s) 148, 178, 361, 404
BseGI GGATG 1 cut(s) 153
BseLI CCNNNNNNNGG 3 cut(s) 40, 103, 316
BseMII CTCAG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 37
Bsh1236I CGCG 4 cut(s) 150, 240, 279, 342
Bsh1285I CGRYCG 2 cut(s) 238, 298
BshFI GGCC 4 cut(s) 44, 170, 197, 257
BshNI GGYRCC 1 cut(s) 329
BshVI ATCGAT 1 cut(s) 354
BsiEI CGRYCG 2 cut(s) 238, 298
BsiHKAI GWGCWC 1 cut(s) 309
BsiSI CCGG 4 cut(s) 41, 180, 298, 310
BslFI GGGAC 1 cut(s) 161
BslI CCNNNNNNNGG 3 cut(s) 40, 103, 316
BsmAI GTCTC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 161
BsmI GAATGC 1 cut(s) 69
BsnI GGCC 4 cut(s) 44, 170, 197, 257
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 2 cut(s) 235, 336
BspACI CCGC 7 cut(s) 51, 87, 148, 150, 264, 277, 340
BspANI GGCC 4 cut(s) 44, 170, 197, 257
BspCNI CTCAG 1 cut(s) 78
BspDI ATCGAT 1 cut(s) 354
BspFNI CGCG 4 cut(s) 150, 240, 279, 342
BspLI GGNNCC 1 cut(s) 331
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 1 cut(s) 331
BspQI GCTCTTC 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 329
BssECI CCNNGG 4 cut(s) 148, 178, 361, 404
BssMI GATC 2 cut(s) 235, 336
BssNI GRCGYC 4 cut(s) 261, 282, 324, 330
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 130, 363
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 86
BstACI GRCGYC 4 cut(s) 261, 282, 324, 330
BstC8I GCNNGC 2 cut(s) 77, 195
BstDEI CTNAG 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 148
BstF5I GGATG 1 cut(s) 153
BstFNI CGCG 4 cut(s) 150, 240, 279, 342
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 2 cut(s) 242, 332
BstKTI GATC 2 cut(s) 238, 339
BstMAI GTCTC 1 cut(s) 420
BstMBI GATC 2 cut(s) 235, 336
BstMCI CGRYCG 2 cut(s) 238, 298
BstMWI GCNNNNNNNGC 3 cut(s) 50, 227, 263
BstNI CCWGG 2 cut(s) 130, 363
BstSCI CCNGG 6 cut(s) 39, 128, 178, 297, 309, 361
BstUI CGCG 4 cut(s) 150, 240, 279, 342
BstX2I RGATCY 1 cut(s) 336
BstYI RGATCY 1 cut(s) 336
Bsu15I ATCGAT 1 cut(s) 354
BsuRI GGCC 4 cut(s) 44, 170, 197, 257
BsuTUI ATCGAT 1 cut(s) 354
BtgI CCRYGG 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 211
BtsCI GGATG 1 cut(s) 153
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 2 cut(s) 135, 437
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 2 cut(s) 77, 195
CfoI GCGC 2 cut(s) 242, 332
Cfr13I GGNCC 1 cut(s) 196
Cfr42I CCGCGG 1 cut(s) 151
ClaI ATCGAT 1 cut(s) 354
CseI GACGC 4 cut(s) 193, 269, 290, 331
Csp6I GTAC 2 cut(s) 118, 145
CspCI CAANNNNNGTGG 2 cut(s) 415, 450
CviAII CATG 1 cut(s) 142
CviJI RGCY 6 cut(s) 44, 170, 183, 197, 257, 307
CviKI_1 RGCY 6 cut(s) 44, 170, 183, 197, 257, 307
CviQI GTAC 2 cut(s) 118, 145
DdeI CTNAG 1 cut(s) 65
DinI GGCGCC 1 cut(s) 331
DpnI GATC 2 cut(s) 237, 338
DpnII GATC 2 cut(s) 235, 336
Eam1104I CTCTTC 1 cut(s) 86
EarI CTCTTC 1 cut(s) 86
EciI GGCGGA 2 cut(s) 40, 76
Ecl136II GAGCTC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 404
Eco147I AGGCCT 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 309
Eco53kI GAGCTC 1 cut(s) 307
EcoICRI GAGCTC 1 cut(s) 307
EcoRII CCWGG 2 cut(s) 128, 361
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 309
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 1 cut(s) 145
FaiI YATR 2 cut(s) 143, 398
FaqI GGGAC 1 cut(s) 161
FatI CATG 1 cut(s) 141
Fnu4HI GCNGC 2 cut(s) 151, 264
FokI GGATG 1 cut(s) 140
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 2 cut(s) 151, 264
GlaI GCGC 2 cut(s) 241, 331
GluI GCNGC 2 cut(s) 151, 264
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 4 cut(s) 44, 170, 197, 257
HapII CCGG 4 cut(s) 41, 180, 298, 310
HgaI GACGC 4 cut(s) 193, 269, 290, 331
HhaI GCGC 2 cut(s) 242, 332
Hin1I GRCGYC 4 cut(s) 261, 282, 324, 330
Hin1II CATG 1 cut(s) 145
Hin6I GCGC 2 cut(s) 240, 330
HinP1I GCGC 2 cut(s) 240, 330
HinfI GANTC 3 cut(s) 111, 125, 356
HpaII CCGG 4 cut(s) 41, 180, 298, 310
HphI GGTGA 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 135
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 1 cut(s) 346
Hpy8I GTNNAC 1 cut(s) 135
Hpy99I CGWCG 5 cut(s) 221, 236, 263, 284, 329
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 227, 263
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 1 cut(s) 324
Hsp92I GRCGYC 4 cut(s) 261, 282, 324, 330
Hsp92II CATG 1 cut(s) 145
HspAI GCGC 2 cut(s) 240, 330
KasI GGCGCC 1 cut(s) 329
KspI CCGCGG 1 cut(s) 151
Kzo9I GATC 2 cut(s) 235, 336
LguI GCTCTTC 1 cut(s) 86
LmnI GCTCC 1 cut(s) 312
LweI GCATC 2 cut(s) 15, 162
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 320
MalI GATC 2 cut(s) 237, 338
MboI GATC 2 cut(s) 235, 336
MboII GAAGA 2 cut(s) 73, 178
MflI RGATCY 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 309
Mly113I GGCGCC 1 cut(s) 330
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 7 cut(s) 55, 58, 64, 74, 181, 295, 299
MseI TTAA 1 cut(s) 272
MslI CAYNNNNRTG 1 cut(s) 140
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 4 cut(s) 41, 180, 298, 310
MspR9I CCNGG 6 cut(s) 41, 130, 180, 299, 311, 363
Mva1269I GAATGC 1 cut(s) 69
MvaI CCWGG 2 cut(s) 130, 363
MvnI CGCG 4 cut(s) 150, 240, 279, 342
MwoI GCNNNNNNNGC 3 cut(s) 50, 227, 263
NarI GGCGCC 1 cut(s) 330
NciI CCSGG 4 cut(s) 41, 180, 299, 311
NdeII GATC 2 cut(s) 235, 336
NlaIII CATG 1 cut(s) 145
NlaIV GGNNCC 1 cut(s) 331
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 320
PceI AGGCCT 1 cut(s) 170
PciSI GCTCTTC 1 cut(s) 86
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 69
PfeI GAWTC 2 cut(s) 111, 356
PflFI GACNNNGTC 1 cut(s) 299
PkrI GCNGC 2 cut(s) 152, 265
Ple19I CGATCG 1 cut(s) 238
PleI GAGTC 1 cut(s) 133
PluTI GGCGCC 1 cut(s) 333
PpsI GAGTC 1 cut(s) 133
PsiI TTATAA 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 2 cut(s) 128, 361
PspGI CCWGG 2 cut(s) 128, 361
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 1 cut(s) 196
PsuI RGATCY 1 cut(s) 336
PsyI GACNNNGTC 1 cut(s) 299
PvuI CGATCG 1 cut(s) 238
RsaI GTAC 2 cut(s) 119, 146
RsaNI GTAC 2 cut(s) 118, 145
RseI CAYNNNNRTG 1 cut(s) 140
SacI GAGCTC 1 cut(s) 309
SacII CCGCGG 1 cut(s) 151
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 2 cut(s) 151, 264
Sau3AI GATC 2 cut(s) 235, 336
Sau96I GGNCC 1 cut(s) 196
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 6 cut(s) 41, 130, 180, 299, 311, 363
SduI GDGCHC 1 cut(s) 309
SetI ASST 6 cut(s) 108, 134, 216, 309, 327, 375
SfaNI GCATC 2 cut(s) 15, 162
SfoI GGCGCC 1 cut(s) 331
Sfr303I CCGCGG 1 cut(s) 151
SgrBI CCGCGG 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 140
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 7 cut(s) 51, 87, 148, 150, 264, 277, 340
SspDI GGCGCC 1 cut(s) 329
SstI GAGCTC 1 cut(s) 309
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 6 cut(s) 39, 128, 178, 297, 309, 361
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 234, 303, 354
TauI GCSGC 2 cut(s) 153, 266
TfiI GAWTC 2 cut(s) 111, 356
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 2 cut(s) 142, 437
TseFI GTSAC 1 cut(s) 320
Tsp45I GTSAC 1 cut(s) 320
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 2 cut(s) 142, 437
Tth111I GACNNNGTC 1 cut(s) 299
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.