Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002349

Physical Location & Seq

Genomic Coordinates Reverse (-)

30796486 .. 30809488

Gene Structure 13003 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1242300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGATATGGAAGCACAGAGGTTTGTATGGGACGGAGCAATTCCTCTGCAGATTCATCTCCATGAATCCGACGTCGCCACTCTTCCTGCTCCCCCACCTGCCCTGATCTTAGCTCCTCGGATTGGATACCTGCCATTGTTGGCCTCACTTCTAAAACCTTACTTCAGCTCTGCACTCCCTCCTCGCTTAGACACCATTTGGTTCGAGTACAAAGGCTTGCCTTTGAAATGGTATATACCGACAGGAGTTCTGTTTGATCTTCTATGTGCGGAACCAGAAAGACCTTGGAATCTCACGGTGCATTTTAGAGGATATCCGGGGAACATATTGATTCCTTGTGATGATGACGATACTGTAAAGTGGAGCTTTATTAATTCTTTGAAAGAGGCTGCATATATAATCAATGGAAACTGTAAGAATGTGATGAACATGTCTCAACCAGATCAGGTGGAGCTCTGGCGTTCAGTCTTAAATGGTAATTTGGAAGCCTACTTCCGGGTATCTTCTAAACTTAAGCTTGGAACAGTTGGGGAAGAATGGGCAGCAAAGAATTTATGCGCTCCAAAATCCAGACCAAATAAGGCCGAAACTGATGTTTCTGGACAAGTGAAGGCAGGTCGAGTTCCAGTTCGCTTATATGTTTGGAGTGTCAGTGAGGATTTTGATGATTTAGAAGATGCACCTCAGATTGATAGCTGGGACAAAGTCTCTTACATTAACCGGCCTGTTGAAATACCAGGAGGAGAAGGAGGTAGATGCTTCACTCTAAATGATGCAATCAAGAGCCTTTTGCCAGAGTATTTTCCTGACAAATCCTTGATCAATGAAGAATCACCGACGGTAGCTGAAGAGGATGAACAAAAGGTTTCCGCTGAAGATGCGAGGGCCGAAGAGGAAGTAGAAAAGTCAGGTGAACAAACAGAGTCTTGCAACCAGTTTGTTGATGCCGAAATCAAGCTAGTCCGTATCCAAGGTATTGAGCCGAAATTGGAGATACCGTTCTCTTGGGTCGCAAACAACCTGCTGAACCCCGAGCACTTTCTTCATATCTGTGTGTATTTGAAAGTACCACAAGTTAGTACCATGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016020 GO:0016043 GO:0016236 GO:0018410 GO:0019538 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034045 GO:0034274 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0098805 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903008 GO:1905037 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04136 ko04137 ko04138 ko04140 ko04211 ko04213 ko04216 ko04621 ko04622 ko05131 map04136 map04137 map04138 map04140 map04211 map04213 map04216 map04621 map04622 map05131

Pfam Domains

Protein Families

APG5

Protein Analysis

364

Amino Acids

40.89

Weight (kDa)

4.71

Isoelectric Point (pI)

41.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG5_UblA	PF20638	9 - 102	6.9e-29	Autophagy protein ATG5, UblA domain
ATG5_HBR	PF20637	117 - 172	1.3e-23	Autophagy protein ATG5, alpha-helical bundle region
ATG5_UblB	PF04106	207 - 352	2.2e-19	Autophagy protein ATG5, UblB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17290
fragaria_vesca	FvH4_5g28670 FvH4_5g28670
malus_domestica	MD08G1242300.v1.1 MD15G1436000.v1.1
prunus_persica	Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1
pyrus_communis	pycom08g21020 pycom15g38480
rosa_chinensis	RchiOBHm_Chr2g0106631 RchiOBHm_Chr5g0080521 RchiOBHm_Chr7g0220631
rosa_laevigata	RLG00000002214 RLG00000017523 RLG00000036864
rosa_multiflora	Rmu_co8045066.1_g000001 Rmu_sc0006221.1_g000004 Rmu_sc0006221.1_g000005 Rmu_ssc0000189.1_g000016
rosa_roxburghii	Rroxscaffold_1G00002880 Rroxscaffold_2G00136950 Rroxscaffold_3G00239270
rosa_rugosa	Rorug02G0136800 Rorug05G0469500 Rorug07G0195300
rosa_samantha	Rh2AG188100 Rh2BG199100 Rh2CG193200 Rh2DG194300 Rh5BG545000 Rh5BG545100 Rh5CG568400 Rh5DG555300 Rh7AG336500 Rh7BG327400 Rh7CG354300 Rh7DG332800
rosa_wichuraiana	Rw0G023170 Rw2G014770 Rw7G028690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	106
AatII	GACGTC	1 cut(s)	75
Acc36I	ACCTGC	4 cut(s)	106, 138, 605, 1029
AciI	CCGC	2 cut(s)	269, 870
AcsI	RAATTY	1 cut(s)	550
AcuI	CTGAAG	3 cut(s)	148, 867, 894
AcyI	GRCGYC	1 cut(s)	72
AfaI	GTAC	3 cut(s)	209, 1068, 1081
AfiI	CCNNNNNNNGG	2 cut(s)	122, 495
AflII	CTTAAG	1 cut(s)	512
AflIII	ACRYGT	1 cut(s)	429
AgsI	TTSAA	4 cut(s)	226, 382, 731, 1063
AjnI	CCWGG	1 cut(s)	736
AloI	GAACNNNNNNTCC	2 cut(s)	983, 1015
AluBI	AGCT	8 cut(s)	113, 168, 366, 454, 517, 696, 845, 958
AluI	AGCT	8 cut(s)	113, 168, 366, 454, 517, 696, 845, 958
Alw21I	GWGCWC	2 cut(s)	456, 1038
Alw26I	GTCTC	2 cut(s)	438, 712
Ama87I	CYCGRG	1 cut(s)	1031
AoxI	GGCC	4 cut(s)	141, 582, 722, 885
ApeKI	GCWGC	2 cut(s)	389, 542
ApoI	RAATTY	1 cut(s)	550
AseI	ATTAAT	1 cut(s)	372
AspLEI	GCGC	1 cut(s)	560
AspS9I	GGNCC	1 cut(s)	885
AsuC2I	CCSGG	2 cut(s)	318, 497
AsuHPI	GGTGA	2 cut(s)	825, 923
AvaI	CYCGRG	1 cut(s)	1031
BanII	GRGCYC	1 cut(s)	456
Bbv12I	GWGCWC	2 cut(s)	456, 1038
BbvI	GCAGC	2 cut(s)	376, 554
BciT130I	CCWGG	1 cut(s)	738
BciVI	GTATCC	2 cut(s)	119, 977
BclI	TGATCA	1 cut(s)	819
BcnI	CCSGG	2 cut(s)	318, 497
BcoDI	GTCTC	2 cut(s)	438, 712
BfaI	CTAG	1 cut(s)	959
BfmI	CTRYAG	1 cut(s)	47
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	4 cut(s)	106, 138, 605, 1029
BfuI	GTATCC	2 cut(s)	119, 977
BisI	GCNGC	2 cut(s)	390, 543
BlsI	GCNGC	2 cut(s)	391, 544
Bme1390I	CCNGG	3 cut(s)	318, 497, 738
BmeT110I	CYCGRG	1 cut(s)	1031
BmgT120I	GGNCC	1 cut(s)	885
BmiI	GGNNCC	1 cut(s)	273
BmrFI	CCNGG	3 cut(s)	318, 497, 738
BmsI	GCATC	5 cut(s)	667, 746, 763, 868, 934
BpuMI	CCSGG	2 cut(s)	318, 497
BsaHI	GRCGYC	1 cut(s)	72
BsaJI	CCNNGG	4 cut(s)	116, 284, 317, 970
BsaXI	ACNNNNNCTCC	4 cut(s)	637, 667, 983, 1013
Bsc4I	CCNNNNNNNGG	2 cut(s)	122, 495
Bse118I	RCCGGY	1 cut(s)	720
Bse1I	ACTGG	2 cut(s)	626, 934
BseBI	CCWGG	1 cut(s)	738
BseDI	CCNNGG	4 cut(s)	116, 284, 317, 970
BseGI	GGATG	1 cut(s)	859
BseLI	CCNNNNNNNGG	2 cut(s)	122, 495
BseMII	CTCAG	1 cut(s)	698
BseNI	ACTGG	2 cut(s)	626, 934
BseRI	GAGGAG	3 cut(s)	105, 171, 756
BseXI	GCAGC	2 cut(s)	376, 554
BseYI	CCCAGC	1 cut(s)	696
BsgI	GTGCAG	1 cut(s)	156
BshFI	GGCC	4 cut(s)	143, 584, 724, 887
BsiHKAI	GWGCWC	2 cut(s)	456, 1038
BsiHKCI	CYCGRG	1 cut(s)	1031
BsiSI	CCGG	3 cut(s)	317, 496, 721
BslFI	GGGAC	2 cut(s)	44, 713
BslI	CCNNNNNNNGG	2 cut(s)	122, 495
BsmAI	GTCTC	2 cut(s)	438, 712
BsmFI	GGGAC	2 cut(s)	44, 713
BsnI	GGCC	4 cut(s)	143, 584, 724, 887
BsoBI	CYCGRG	1 cut(s)	1031
Bsp1286I	GDGCHC	2 cut(s)	456, 1038
Bsp143I	GATC	4 cut(s)	105, 256, 442, 819
BspACI	CCGC	2 cut(s)	269, 870
BspANI	GGCC	4 cut(s)	143, 584, 724, 887
BspCNI	CTCAG	1 cut(s)	697
BspLI	GGNNCC	1 cut(s)	273
BspMAI	CTGCAG	1 cut(s)	51
BspMI	ACCTGC	4 cut(s)	106, 138, 605, 1029
BspTI	CTTAAG	1 cut(s)	512
BsrFI	RCCGGY	1 cut(s)	720
BsrI	ACTGG	2 cut(s)	626, 934
BssAI	RCCGGY	1 cut(s)	720
BssECI	CCNNGG	4 cut(s)	116, 284, 317, 970
BssMI	GATC	4 cut(s)	105, 256, 442, 819
BssNI	GRCGYC	1 cut(s)	72
BssT1I	CCWWGG	2 cut(s)	284, 970
Bst2UI	CCWGG	1 cut(s)	738
Bst4CI	ACNGT	6 cut(s)	298, 355, 413, 526, 841, 999
Bst6I	CTCTTC	3 cut(s)	87, 843, 885
BstACI	GRCGYC	1 cut(s)	72
BstAFI	CTTAAG	1 cut(s)	512
BstC8I	GCNNGC	1 cut(s)	218
BstDEI	CTNAG	3 cut(s)	109, 187, 684
BstF5I	GGATG	1 cut(s)	859
BstHHI	GCGC	1 cut(s)	560
BstKTI	GATC	4 cut(s)	108, 259, 445, 822
BstMAI	GTCTC	2 cut(s)	438, 712
BstMBI	GATC	4 cut(s)	105, 256, 442, 819
BstMWI	GCNNNNNNNGC	1 cut(s)	878
BstNI	CCWGG	1 cut(s)	738
BstNSI	RCATGY	1 cut(s)	433
BstSCI	CCNGG	3 cut(s)	316, 495, 736
BstSFI	CTRYAG	1 cut(s)	47
BstV1I	GCAGC	2 cut(s)	376, 554
BsuI	GTATCC	2 cut(s)	119, 977
BsuRI	GGCC	4 cut(s)	143, 584, 724, 887
BtsCI	GGATG	1 cut(s)	859
BtsIMutI	CAGTG	1 cut(s)	658
BveI	ACCTGC	4 cut(s)	106, 138, 605, 1029
Cac8I	GCNNGC	1 cut(s)	218
CfoI	GCGC	1 cut(s)	560
Cfr10I	RCCGGY	1 cut(s)	720
Cfr13I	GGNCC	1 cut(s)	885
Csp6I	GTAC	3 cut(s)	208, 1067, 1080
CviAII	CATG	3 cut(s)	62, 430, 1084
CviQI	GTAC	3 cut(s)	208, 1067, 1080
DdeI	CTNAG	3 cut(s)	109, 187, 684
DpnI	GATC	4 cut(s)	107, 258, 444, 821
DpnII	GATC	4 cut(s)	105, 256, 442, 819
Eam1104I	CTCTTC	3 cut(s)	87, 843, 885
EarI	CTCTTC	3 cut(s)	87, 843, 885
Ecl136II	GAGCTC	1 cut(s)	454
Eco130I	CCWWGG	2 cut(s)	284, 970
Eco24I	GRGCYC	1 cut(s)	456
Eco32I	GATATC	1 cut(s)	314
Eco53kI	GAGCTC	1 cut(s)	454
Eco57I	CTGAAG	3 cut(s)	148, 867, 894
Eco88I	CYCGRG	1 cut(s)	1031
EcoICRI	GAGCTC	1 cut(s)	454
EcoRII	CCWGG	1 cut(s)	736
EcoRV	GATATC	1 cut(s)	314
EcoT14I	CCWWGG	2 cut(s)	284, 970
EcoT38I	GRGCYC	1 cut(s)	456
ErhI	CCWWGG	2 cut(s)	284, 970
FaeI	CATG	3 cut(s)	65, 433, 1087
FalI	AAGNNNNNCTT	2 cut(s)	350, 382
FaqI	GGGAC	2 cut(s)	44, 713
FatI	CATG	3 cut(s)	61, 429, 1083
FbaI	TGATCA	1 cut(s)	819
Fnu4HI	GCNGC	2 cut(s)	390, 543
FokI	GGATG	1 cut(s)	866
FriOI	GRGCYC	1 cut(s)	456
Fsp4HI	GCNGC	2 cut(s)	390, 543
FspBI	CTAG	1 cut(s)	959
GlaI	GCGC	1 cut(s)	559
GluI	GCNGC	2 cut(s)	390, 543
GsaI	CCCAGC	1 cut(s)	700
HaeIII	GGCC	4 cut(s)	143, 584, 724, 887
HapII	CCGG	3 cut(s)	317, 496, 721
HhaI	GCGC	1 cut(s)	560
Hin1I	GRCGYC	1 cut(s)	72
Hin1II	CATG	3 cut(s)	65, 433, 1087
Hin6I	GCGC	1 cut(s)	558
HinP1I	GCGC	1 cut(s)	558
HindIII	AAGCTT	1 cut(s)	515
HinfI	GANTC	6 cut(s)	52, 65, 289, 331, 830, 923
HpaII	CCGG	3 cut(s)	317, 496, 721
HphI	GGTGA	2 cut(s)	825, 923
Hpy166II	GTNNAC	1 cut(s)	914
Hpy188I	TCNGA	3 cut(s)	70, 120, 687
Hpy188III	TCNNGA	4 cut(s)	570, 600, 781, 806
Hpy8I	GTNNAC	1 cut(s)	914
Hpy99I	CGWCG	3 cut(s)	74, 77, 841
HpyAV	CCTTC	2 cut(s)	604, 740
HpyCH4III	ACNGT	6 cut(s)	298, 355, 413, 526, 841, 999
HpyCH4IV	ACGT	1 cut(s)	72
HpyCH4V	TGCA	7 cut(s)	49, 173, 301, 392, 680, 776, 930
HpyF10VI	GCNNNNNNNGC	1 cut(s)	878
HpyF3I	CTNAG	3 cut(s)	109, 187, 684
HpySE526I	ACGT	1 cut(s)	72
Hsp92I	GRCGYC	1 cut(s)	72
Hsp92II	CATG	3 cut(s)	65, 433, 1087
HspAI	GCGC	1 cut(s)	558
Ksp22I	TGATCA	1 cut(s)	819
Kzo9I	GATC	4 cut(s)	105, 256, 442, 819
LmnI	GCTCC	6 cut(s)	35, 94, 118, 363, 451, 565
Lsp1109I	GCAGC	2 cut(s)	376, 554
LweI	GCATC	5 cut(s)	667, 746, 763, 868, 934
MaeI	CTAG	1 cut(s)	959
MaeII	ACGT	1 cut(s)	72
MalI	GATC	4 cut(s)	107, 258, 444, 821
MboI	GATC	4 cut(s)	105, 256, 442, 819
MhlI	GDGCHC	2 cut(s)	456, 1038
MluCI	AATT	5 cut(s)	39, 373, 478, 550, 986
MlyI	GAGTC	1 cut(s)	932
MmeI	TCCRAC	1 cut(s)	93
MseI	TTAA	4 cut(s)	372, 470, 513, 717
MslI	CAYNNNNRTG	2 cut(s)	60, 1050
MspA1I	CMGCKG	1 cut(s)	872
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	3 cut(s)	317, 496, 721
MspR9I	CCNGG	3 cut(s)	318, 497, 738
MvaI	CCWGG	1 cut(s)	738
MwoI	GCNNNNNNNGC	1 cut(s)	878
NciI	CCSGG	2 cut(s)	318, 497
NdeII	GATC	4 cut(s)	105, 256, 442, 819
NlaIII	CATG	3 cut(s)	65, 433, 1087
NlaIV	GGNNCC	1 cut(s)	273
NspI	RCATGY	1 cut(s)	433
PaqCI	CACCTGC	1 cut(s)	106
PciI	ACATGT	1 cut(s)	429
PfeI	GAWTC	5 cut(s)	52, 65, 289, 331, 830
PflFI	GACNNNGTC	1 cut(s)	704
PkrI	GCNGC	2 cut(s)	391, 544
PleI	GAGTC	1 cut(s)	931
PpsI	GAGTC	1 cut(s)	931
PscI	ACATGT	1 cut(s)	429
PshBI	ATTAAT	1 cut(s)	372
Psp124BI	GAGCTC	1 cut(s)	456
Psp6I	CCWGG	1 cut(s)	736
PspFI	CCCAGC	1 cut(s)	696
PspGI	CCWGG	1 cut(s)	736
PspN4I	GGNNCC	1 cut(s)	273
PspPI	GGNCC	1 cut(s)	885
PstI	CTGCAG	1 cut(s)	51
PsyI	GACNNNGTC	1 cut(s)	704
RsaI	GTAC	3 cut(s)	209, 1068, 1081
RsaNI	GTAC	3 cut(s)	208, 1067, 1080
RseI	CAYNNNNRTG	2 cut(s)	60, 1050
SacI	GAGCTC	1 cut(s)	456
SaqAI	TTAA	4 cut(s)	372, 470, 513, 717
SatI	GCNGC	2 cut(s)	390, 543
Sau3AI	GATC	4 cut(s)	105, 256, 442, 819
Sau96I	GGNCC	1 cut(s)	885
SchI	GAGTC	1 cut(s)	932
ScrFI	CCNGG	3 cut(s)	318, 497, 738
SduI	GDGCHC	2 cut(s)	456, 1038
SfaNI	GCATC	5 cut(s)	667, 746, 763, 868, 934
SfcI	CTRYAG	1 cut(s)	47
SmiMI	CAYNNNNRTG	2 cut(s)	60, 1050
SmlI	CTYRAG	1 cut(s)	512
SmoI	CTYRAG	1 cut(s)	512
Sse9I	AATT	5 cut(s)	39, 373, 478, 550, 986
SsiI	CCGC	2 cut(s)	269, 870
SspMI	CTAG	1 cut(s)	959
SstI	GAGCTC	1 cut(s)	456
StyD4I	CCNGG	3 cut(s)	316, 495, 736
StyI	CCWWGG	2 cut(s)	284, 970
TaaI	ACNGT	6 cut(s)	298, 355, 413, 526, 841, 999
TaiI	ACGT	1 cut(s)	75
TaqI	TCGA	2 cut(s)	204, 619
TasI	AATT	5 cut(s)	39, 373, 478, 550, 986
TatI	WGTACW	1 cut(s)	207
TfiI	GAWTC	5 cut(s)	52, 65, 289, 331, 830
Tru1I	TTAA	4 cut(s)	372, 470, 513, 717
Tru9I	TTAA	4 cut(s)	372, 470, 513, 717
TscAI	CASTG	1 cut(s)	658
TseI	GCWGC	2 cut(s)	389, 542
TspDTI	ATGAA	6 cut(s)	44, 78, 440, 840, 870, 1034
TspGWI	ACGGA	2 cut(s)	48, 953
TspRI	CASTG	1 cut(s)	658
Tth111I	GACNNNGTC	1 cut(s)	704
Vha464I	CTTAAG	1 cut(s)	512
VspI	ATTAAT	1 cut(s)	372
XapI	RAATTY	1 cut(s)	550
XceI	RCATGY	1 cut(s)	433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	282
XspI	CTAG	1 cut(s)	959
ZraI	GACGTC	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.