Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002349

Physical Location & Seq

Genomic Coordinates Reverse (-)

29713028 .. 29718254

Gene Structure 5227 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000002214

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGGACATGGAAGCACAGAGGTTTTTATGGGAAGGAGCAATTCCTTTGCAGATTCATCTCCACGAATCCGAAGTTACGACTCTACCTTCTCCCCCGCCCGCCCTGATCTTAGCTCCTCGGCTAGGGTACCTACCATTGTTGGCCTCGCATCTGAAGCCTTACTTCAGCACTGCACTTCCTCCTGGTGTTGACACTATTTGGTTCGACTACAAAGGCTTGCCCTTGAAATGGTATATACCAACAGGAGTTCTTTTTGATCTTCTTTGTGCAGAACCAGAGAGACCTTGGAATTTAACGGTTCATTTTAGAGGATATCCAGGAAACATATTGATCCCTTGTGAAGGTGAAGATAGCATAAAGTGGAACTTTATTAACTCATTGAAAGAGGCAGCATATATAATCAATGGAAACTGCAAGAATGTGATGAACATGTCTCAACCTGATCAGGTGGAGCTCTGGCGCTCTGTCTTAAATGGTAATTTGGAAGCCTACCGACGGGTATCTTCTAAACTTAAGCTTGAAGCCGTTGGGGAAATGAACTCGAGCTCGCCAAGATCCCGATCGAGTGTGGGTGAAGTTGATGTTTCTGGGCAGGTGAAGCCAGGCCGAGTTCCAGTTCGGTTATATGTTTGGAATGTCAGTGAGGACTTTGATGACTTAGAAGATGCACCACCGATTGAAAGTTGGGACAAAGTTTCTTACATAAACCGGCCTGTCGAGATACAAGAAGGTAGATGCTTCACTTTACATGATGCAGTGAAGAGCCTTCTGCCAGACTATTTTGTGGTCAAATCCTTGGGCACTGCTGAATCACAGACAGTAGCTGGTGATGATGAGCAGAGAGTTTCATCTGAAGATGCAAGTGATGATAAAAAAGCTGAAGAGGAAGTAGAAACTTCATCTGATGACCATGTAAAGGCTAGTTATGAATATGAAGAGCCCGAAATCAAGCTGGTTCGTATACAAGGTATTGAACCAAAACTGGAGATACCATTTTCTTGGGTGGCAAACAACTTAATGCACCCTGAGCACTTTCTTCATATTTGTGTATATTTGAAAGTACCACAAGTCAATAATACTATGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016020 GO:0016043 GO:0016236 GO:0018410 GO:0019538 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034045 GO:0034274 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0098805 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903008 GO:1905037 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04136 ko04137 ko04138 ko04140 ko04211 ko04213 ko04216 ko04621 ko04622 ko05131 map04136 map04137 map04138 map04140 map04211 map04213 map04216 map04621 map04622 map05131

Pfam Domains

Protein Families

APG5

Protein Analysis

363

Amino Acids

40.95

Weight (kDa)

4.84

Isoelectric Point (pI)

47.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG5_UblA	PF20638	9 - 102	4.7e-29	Autophagy protein ATG5, UblA domain
ATG5_HBR	PF20637	116 - 172	1.7e-23	Autophagy protein ATG5, alpha-helical bundle region
ATG5_UblB	PF04106	203 - 350	3.1e-19	Autophagy protein ATG5, UblB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17290
fragaria_vesca	FvH4_5g28670 FvH4_5g28670
malus_domestica	MD08G1242300.v1.1 MD15G1436000.v1.1
prunus_persica	Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1
pyrus_communis	pycom08g21020 pycom15g38480
rosa_chinensis	RchiOBHm_Chr2g0106631 RchiOBHm_Chr5g0080521 RchiOBHm_Chr7g0220631
rosa_laevigata	RLG00000002214 RLG00000017523 RLG00000036864
rosa_multiflora	Rmu_co8045066.1_g000001 Rmu_sc0006221.1_g000004 Rmu_sc0006221.1_g000005 Rmu_ssc0000189.1_g000016
rosa_roxburghii	Rroxscaffold_1G00002880 Rroxscaffold_2G00136950 Rroxscaffold_3G00239270
rosa_rugosa	Rorug02G0136800 Rorug05G0469500 Rorug07G0195300
rosa_samantha	Rh2AG188100 Rh2BG199100 Rh2CG193200 Rh2DG194300 Rh5BG545000 Rh5BG545100 Rh5CG568400 Rh5DG555300 Rh7AG336500 Rh7BG327400 Rh7CG354300 Rh7DG332800
rosa_wichuraiana	Rw0G023170 Rw2G014770 Rw7G028690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	583
Acc36I	ACCTGC	1 cut(s)	583
Acc65I	GGTACC	1 cut(s)	126
AccB1I	GGYRCC	1 cut(s)	126
AccI	GTMKAC	1 cut(s)	961
AciI	CCGC	2 cut(s)	95, 99
AclWI	GGATC	2 cut(s)	325, 549
AcsI	RAATTY	1 cut(s)	289
AcuI	CTGAAG	4 cut(s)	148, 173, 873, 900
AfaI	GTAC	2 cut(s)	128, 1062
AfiI	CCNNNNNNNGG	5 cut(s)	122, 228, 341, 495, 916
AflII	CTTAAG	1 cut(s)	512
AflIII	ACRYGT	1 cut(s)	429
AgsI	TTSAA	6 cut(s)	226, 382, 521, 680, 974, 1057
AjnI	CCWGG	3 cut(s)	181, 316, 601
AluBI	AGCT	7 cut(s)	113, 454, 517, 546, 824, 878, 952
AluI	AGCT	7 cut(s)	113, 454, 517, 546, 824, 878, 952
Alw21I	GWGCWC	3 cut(s)	456, 548, 1032
Alw26I	GTCTC	2 cut(s)	274, 438
AlwI	GGATC	2 cut(s)	325, 549
AlwNI	CAGNNNCTG	1 cut(s)	824
Ama87I	CYCGRG	1 cut(s)	541
AoxI	GGCC	3 cut(s)	141, 604, 710
ApeKI	GCWGC	1 cut(s)	389
ApoI	RAATTY	1 cut(s)	289
Asp718I	GGTACC	1 cut(s)	126
AspLEI	GCGC	1 cut(s)	462
AsuHPI	GGTGA	4 cut(s)	356, 584, 607, 839
AvaI	CYCGRG	1 cut(s)	541
BaeGI	GKGCMC	1 cut(s)	803
BanI	GGYRCC	1 cut(s)	126
BanII	GRGCYC	3 cut(s)	456, 548, 942
Bbv12I	GWGCWC	3 cut(s)	456, 548, 1032
BbvI	GCAGC	1 cut(s)	401
BceAI	ACGGC	1 cut(s)	509
BciT130I	CCWGG	3 cut(s)	183, 318, 603
BclI	TGATCA	1 cut(s)	442
BcoDI	GTCTC	2 cut(s)	274, 438
BfaI	CTAG	2 cut(s)	122, 921
BfoI	RGCGCY	1 cut(s)	463
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	1 cut(s)	583
BisI	GCNGC	1 cut(s)	390
BlsI	GCNGC	1 cut(s)	391
Bme1390I	CCNGG	3 cut(s)	183, 318, 603
BmeT110I	CYCGRG	1 cut(s)	541
BmiI	GGNNCC	1 cut(s)	128
BmrFI	CCNGG	3 cut(s)	183, 318, 603
BmsI	GCATC	5 cut(s)	157, 655, 725, 742, 847
BpmI	CTGGAG	1 cut(s)	1004
Bpu10I	CCTNAGC	1 cut(s)	1026
BsaBI	GATNNNNATC	1 cut(s)	559
BsaI	GGTCTC	1 cut(s)	274
BsaJI	CCNNGG	3 cut(s)	116, 284, 795
Bsc4I	CCNNNNNNNGG	5 cut(s)	122, 228, 341, 495, 916
Bse118I	RCCGGY	1 cut(s)	708
Bse1I	ACTGG	2 cut(s)	614, 987
Bse8I	GATNNNNATC	1 cut(s)	559
BseBI	CCWGG	3 cut(s)	183, 318, 603
BseDI	CCNNGG	3 cut(s)	116, 284, 795
BseJI	GATNNNNATC	1 cut(s)	559
BseLI	CCNNNNNNNGG	5 cut(s)	122, 228, 341, 495, 916
BseMII	CTCAG	1 cut(s)	1017
BseNI	ACTGG	2 cut(s)	614, 987
BseRI	GAGGAG	1 cut(s)	105
BseSI	GKGCMC	1 cut(s)	803
BseXI	GCAGC	1 cut(s)	401
BsgI	GTGCAG	2 cut(s)	156, 288
Bsh1285I	CGRYCG	1 cut(s)	563
BshFI	GGCC	3 cut(s)	143, 606, 712
BshNI	GGYRCC	1 cut(s)	126
BsiEI	CGRYCG	1 cut(s)	563
BsiHKAI	GWGCWC	3 cut(s)	456, 548, 1032
BsiHKCI	CYCGRG	1 cut(s)	541
BsiSI	CCGG	1 cut(s)	709
BslFI	GGGAC	1 cut(s)	701
BslI	CCNNNNNNNGG	5 cut(s)	122, 228, 341, 495, 916
BsmAI	GTCTC	2 cut(s)	274, 438
BsmFI	GGGAC	1 cut(s)	701
BsnI	GGCC	3 cut(s)	143, 606, 712
Bso31I	GGTCTC	1 cut(s)	274
BsoBI	CYCGRG	1 cut(s)	541
Bsp1286I	GDGCHC	5 cut(s)	456, 548, 803, 942, 1032
Bsp143I	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
BspACI	CCGC	2 cut(s)	95, 99
BspANI	GGCC	3 cut(s)	143, 606, 712
BspCNI	CTCAG	1 cut(s)	1018
BspLI	GGNNCC	1 cut(s)	128
BspMI	ACCTGC	1 cut(s)	583
BspPI	GGATC	2 cut(s)	325, 549
BspQI	GCTCTTC	2 cut(s)	755, 930
BspT107I	GGYRCC	1 cut(s)	126
BspTI	CTTAAG	1 cut(s)	512
BspTNI	GGTCTC	1 cut(s)	274
BsrFI	RCCGGY	1 cut(s)	708
BsrI	ACTGG	2 cut(s)	614, 987
BssAI	RCCGGY	1 cut(s)	708
BssECI	CCNNGG	3 cut(s)	116, 284, 795
BssMI	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
BssNAI	GTATAC	1 cut(s)	962
BssT1I	CCWWGG	2 cut(s)	284, 795
Bst1107I	GTATAC	1 cut(s)	962
Bst2UI	CCWGG	3 cut(s)	183, 318, 603
Bst4CI	ACNGT	2 cut(s)	298, 820
Bst6I	CTCTTC	3 cut(s)	755, 876, 930
BstAFI	CTTAAG	1 cut(s)	512
BstC8I	GCNNGC	3 cut(s)	99, 218, 548
BstDEI	CTNAG	3 cut(s)	109, 658, 1026
BstENI	CCTNNNNNAGG	2 cut(s)	120, 339
BstH2I	RGCGCY	1 cut(s)	463
BstHHI	GCGC	1 cut(s)	462
BstKTI	GATC	6 cut(s)	108, 259, 333, 445, 557, 563
BstMAI	GTCTC	2 cut(s)	274, 438
BstMBI	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
BstMCI	CGRYCG	1 cut(s)	563
BstMWI	GCNNNNNNNGC	3 cut(s)	154, 598, 1027
BstNI	CCWGG	3 cut(s)	183, 318, 603
BstNSI	RCATGY	1 cut(s)	433
BstSCI	CCNGG	3 cut(s)	181, 316, 601
BstSLI	GKGCMC	1 cut(s)	803
BstV1I	GCAGC	1 cut(s)	401
BstX2I	RGATCY	1 cut(s)	554
BstXI	CCANNNNNNTGG	1 cut(s)	999
BstYI	RGATCY	1 cut(s)	554
BstZ17I	GTATAC	1 cut(s)	962
BsuRI	GGCC	3 cut(s)	143, 606, 712
BtsI	GCAGTG	3 cut(s)	168, 762, 801
BtsIMutI	CAGTG	4 cut(s)	168, 646, 762, 801
BveI	ACCTGC	1 cut(s)	583
Cac8I	GCNNGC	3 cut(s)	99, 218, 548
CaiI	CAGNNNCTG	1 cut(s)	824
CfoI	GCGC	1 cut(s)	462
Cfr10I	RCCGGY	1 cut(s)	708
Csp6I	GTAC	2 cut(s)	127, 1061
CviAII	CATG	4 cut(s)	7, 430, 749, 911
CviQI	GTAC	2 cut(s)	127, 1061
DdeI	CTNAG	3 cut(s)	109, 658, 1026
DpnI	GATC	6 cut(s)	107, 258, 332, 444, 556, 562
DpnII	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
Eam1104I	CTCTTC	3 cut(s)	755, 876, 930
EarI	CTCTTC	3 cut(s)	755, 876, 930
Ecl136II	GAGCTC	2 cut(s)	454, 546
Eco130I	CCWWGG	2 cut(s)	284, 795
Eco24I	GRGCYC	3 cut(s)	456, 548, 942
Eco31I	GGTCTC	1 cut(s)	274
Eco32I	GATATC	1 cut(s)	314
Eco53kI	GAGCTC	2 cut(s)	454, 546
Eco57I	CTGAAG	4 cut(s)	148, 173, 873, 900
Eco88I	CYCGRG	1 cut(s)	541
EcoICRI	GAGCTC	2 cut(s)	454, 546
EcoNI	CCTNNNNNAGG	2 cut(s)	120, 339
EcoRII	CCWGG	3 cut(s)	181, 316, 601
EcoRV	GATATC	1 cut(s)	314
EcoT14I	CCWWGG	2 cut(s)	284, 795
EcoT38I	GRGCYC	3 cut(s)	456, 548, 942
ErhI	CCWWGG	2 cut(s)	284, 795
FaeI	CATG	4 cut(s)	10, 433, 752, 914
FalI	AAGNNNNNCTT	4 cut(s)	146, 178, 350, 382
FaqI	GGGAC	1 cut(s)	701
FatI	CATG	4 cut(s)	6, 429, 748, 910
FauI	CCCGC	2 cut(s)	102, 106
FbaI	TGATCA	1 cut(s)	442
FblI	GTMKAC	1 cut(s)	961
Fnu4HI	GCNGC	1 cut(s)	390
FriOI	GRGCYC	3 cut(s)	456, 548, 942
Fsp4HI	GCNGC	1 cut(s)	390
FspBI	CTAG	2 cut(s)	122, 921
GlaI	GCGC	1 cut(s)	461
GluI	GCNGC	1 cut(s)	390
GsuI	CTGGAG	1 cut(s)	1004
HaeII	RGCGCY	1 cut(s)	463
HaeIII	GGCC	3 cut(s)	143, 606, 712
HapII	CCGG	1 cut(s)	709
HhaI	GCGC	1 cut(s)	462
Hin1II	CATG	4 cut(s)	10, 433, 752, 914
Hin6I	GCGC	1 cut(s)	460
HinP1I	GCGC	1 cut(s)	460
HincII	GTYRAC	1 cut(s)	190
HindII	GTYRAC	1 cut(s)	190
HindIII	AAGCTT	1 cut(s)	515
HinfI	GANTC	4 cut(s)	52, 65, 79, 809
HpaII	CCGG	1 cut(s)	709
HphI	GGTGA	4 cut(s)	356, 584, 607, 839
Hpy166II	GTNNAC	2 cut(s)	190, 962
Hpy188I	TCNGA	4 cut(s)	70, 153, 853, 904
Hpy188III	TCNNGA	2 cut(s)	558, 718
Hpy8I	GTNNAC	2 cut(s)	190, 962
Hpy99I	CGWCG	1 cut(s)	498
HpyAV	CCTTC	5 cut(s)	26, 96, 335, 722, 776
HpyCH4III	ACNGT	2 cut(s)	298, 820
HpyCH4V	TGCA	8 cut(s)	49, 173, 269, 414, 668, 755, 860, 1021
HpyF10VI	GCNNNNNNNGC	3 cut(s)	154, 598, 1027
HpyF3I	CTNAG	3 cut(s)	109, 658, 1026
Hsp92II	CATG	4 cut(s)	10, 433, 752, 914
HspAI	GCGC	1 cut(s)	460
KpnI	GGTACC	1 cut(s)	130
Ksp22I	TGATCA	1 cut(s)	442
Kzo9I	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
LguI	GCTCTTC	2 cut(s)	755, 930
LmnI	GCTCC	3 cut(s)	35, 118, 451
Lsp1109I	GCAGC	1 cut(s)	401
LweI	GCATC	5 cut(s)	157, 655, 725, 742, 847
MaeI	CTAG	2 cut(s)	122, 921
MaeIII	GTNAC	1 cut(s)	73
MalI	GATC	6 cut(s)	107, 258, 332, 444, 556, 562
MboI	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
MboII	GAAGA	9 cut(s)	251, 359, 495, 674, 772, 866, 893, 947, 1028
MflI	RGATCY	1 cut(s)	554
MhlI	GDGCHC	5 cut(s)	456, 548, 803, 942, 1032
MluCI	AATT	3 cut(s)	39, 289, 478
MlyI	GAGTC	1 cut(s)	73
MnlI	CCTC	8 cut(s)	12, 126, 154, 189, 302, 379, 637, 877
MseI	TTAA	5 cut(s)	293, 372, 470, 513, 1016
MslI	CAYNNNNRTG	1 cut(s)	1044
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	1 cut(s)	709
MspR9I	CCNGG	3 cut(s)	183, 318, 603
MvaI	CCWGG	3 cut(s)	183, 318, 603
MwoI	GCNNNNNNNGC	3 cut(s)	154, 598, 1027
NdeII	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
NlaIII	CATG	4 cut(s)	10, 433, 752, 914
NlaIV	GGNNCC	1 cut(s)	128
NmeAIII	GCCGAG	2 cut(s)	97, 632
NspI	RCATGY	1 cut(s)	433
PaeR7I	CTCGAG	1 cut(s)	541
PaqCI	CACCTGC	1 cut(s)	583
PciI	ACATGT	1 cut(s)	429
PciSI	GCTCTTC	2 cut(s)	755, 930
PfeI	GAWTC	3 cut(s)	52, 65, 809
PfoI	TCCNGGA	1 cut(s)	316
PkrI	GCNGC	1 cut(s)	391
Ple19I	CGATCG	1 cut(s)	563
PleI	GAGTC	1 cut(s)	73
PpsI	GAGTC	1 cut(s)	73
PscI	ACATGT	1 cut(s)	429
Psp124BI	GAGCTC	2 cut(s)	456, 548
Psp6I	CCWGG	3 cut(s)	181, 316, 601
PspGI	CCWGG	3 cut(s)	181, 316, 601
PspN4I	GGNNCC	1 cut(s)	128
PspXI	VCTCGAGB	1 cut(s)	541
PstNI	CAGNNNCTG	1 cut(s)	824
PsuI	RGATCY	1 cut(s)	554
PvuI	CGATCG	1 cut(s)	563
RsaI	GTAC	2 cut(s)	128, 1062
RsaNI	GTAC	2 cut(s)	127, 1061
RseI	CAYNNNNRTG	1 cut(s)	1044
SacI	GAGCTC	2 cut(s)	456, 548
SapI	GCTCTTC	2 cut(s)	755, 930
SaqAI	TTAA	5 cut(s)	293, 372, 470, 513, 1016
SatI	GCNGC	1 cut(s)	390
Sau3AI	GATC	6 cut(s)	105, 256, 330, 442, 554, 560
SchI	GAGTC	1 cut(s)	73
ScrFI	CCNGG	3 cut(s)	183, 318, 603
SduI	GDGCHC	5 cut(s)	456, 548, 803, 942, 1032
SfaNI	GCATC	5 cut(s)	157, 655, 725, 742, 847
Sfr274I	CTCGAG	1 cut(s)	541
SlaI	CTCGAG	1 cut(s)	541
SmiMI	CAYNNNNRTG	1 cut(s)	1044
SmlI	CTYRAG	2 cut(s)	512, 541
SmoI	CTYRAG	2 cut(s)	512, 541
Sse9I	AATT	3 cut(s)	39, 289, 478
SsiI	CCGC	2 cut(s)	95, 99
SspMI	CTAG	2 cut(s)	122, 921
SstI	GAGCTC	2 cut(s)	456, 548
StyD4I	CCNGG	3 cut(s)	181, 316, 601
StyI	CCWWGG	2 cut(s)	284, 795
TaaI	ACNGT	2 cut(s)	298, 820
TaqI	TCGA	4 cut(s)	204, 542, 563, 717
TasI	AATT	3 cut(s)	39, 289, 478
TfiI	GAWTC	3 cut(s)	52, 65, 809
Tru1I	TTAA	5 cut(s)	293, 372, 470, 513, 1016
Tru9I	TTAA	5 cut(s)	293, 372, 470, 513, 1016
TscAI	CASTG	4 cut(s)	175, 646, 762, 808
TseI	GCWGC	1 cut(s)	389
TspDTI	ATGAA	9 cut(s)	44, 290, 440, 551, 837, 888, 942, 948, 1028
TspRI	CASTG	4 cut(s)	175, 646, 762, 808
Vha464I	CTTAAG	1 cut(s)	512
XagI	CCTNNNNNAGG	2 cut(s)	120, 339
XapI	RAATTY	1 cut(s)	289
XceI	RCATGY	1 cut(s)	433
XcmI	CCANNNNNNNNNTGG	1 cut(s)	282
XhoI	CTCGAG	1 cut(s)	541
XmiI	GTMKAC	1 cut(s)	961
XspI	CTAG	2 cut(s)	122, 921

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.