Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002349

Physical Location & Seq

Genomic Coordinates Reverse (-)

53626435 .. 53632657

Gene Structure 6223 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1436000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGGATATGGAAGCACAGAGGTTTGTATGGGGCGGAGCAATTCCTCTGCAGATTCATCTCCATGAATCCGACGTCGCCACTCTTCCTGCTCCCCCACCTGCCCTGATCTTAGCTCCTCGGATTGGGTATCTGCCATTGTTGGCCTCGCTTCTAAAACCTCACTTCAGCTCTGCACTTCCTCCTCGCTTAGACACCATTTGGTTCGAGTACAAAGGCTTGCCCTTGAAATGGTATATACCGACAGGAGTTCTGTTTGATCTTCTATGTGCAGAGCCAGAAAGACCTTGGAATCTCACGGTGCATTTTACAGGATATCCGGGGAACATATTGATTCCTTGTGATGGTGAAGATAGTGTAAAGTGGAGCTTTATCAATTCTTTGAAAGAGGCTGCATATATAATAAATGGAAACTGTAAGAATGTGATGAACATGTCTCAGCCAGATCAGGTGGAGCTTTGGCGTTCGGTCTTAAATGGTAATTTGGAAGCCTACTTCCGGGTATCTTCTAAACTTAAGCTTGGAACAGTTGGGGAAGACTGGGCAGCAAAGAATTCATGCTCTCCAAAATCCAGACCAAATATGGGCGAAACTGATGTTTCTGGACAAGTGAAGGCAGGTCGTGTTCCAGTTCGTTTATATGTTTGGAGTGTCAGTGAGGATTTTGATGATTTAGAAGATGCACCTCAGATTGATAGTTGGGACAAAGTCTCTTACATTAACCGGCCTGTTGAGATACAAGGAGGAGGAGGTAGATGCTTCACTCTAAATGATGCAATCAAGAGCCTTTTGCCAGAGTATTTTCCTGACAAATCCATGATCCCTGAAGAATCACCAACAGTAGGTGAGGAGGATGAACAAAAGGTTTCCTCTGAAGATGCAATCAAGAGCGATATAGGAGCCGAAGAGGAAGTAGAAAAGTCAATCGAACGCACAAAGTCTTGCAACCAATACGATGATGCTGAAATCAAACTAGTCCGTATCCAAGGTATTGAGCCGAAATTGGAGATACCTTTCTTTTGGGTGGCAAATAACTTACTGAACCCCGAGCACTTTCTTCATATCTGTGTGTATTTGAAAGTACCACAAATCAATAACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000045 GO:0000407 GO:0000422 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006497 GO:0006501 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006995 GO:0006996 GO:0007005 GO:0007033 GO:0007154 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009059 GO:0009267 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0009991 GO:0010150 GO:0016020 GO:0016043 GO:0016236 GO:0018410 GO:0019538 GO:0022411 GO:0022607 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0032991 GO:0033554 GO:0034045 GO:0034274 GO:0034645 GO:0036211 GO:0042157 GO:0042158 GO:0042594 GO:0043170 GO:0043207 GO:0043412 GO:0043562 GO:0043687 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044804 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061726 GO:0061919 GO:0070925 GO:0071496 GO:0071704 GO:0071840 GO:0090693 GO:0098542 GO:0098805 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1902494 GO:1903008 GO:1905037 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko04136 ko04137 ko04138 ko04140 ko04211 ko04213 ko04216 ko04621 ko04622 ko05131 map04136 map04137 map04138 map04140 map04211 map04213 map04216 map04621 map04622 map05131

Pfam Domains

Protein Families

APG5

Protein Analysis

367

Amino Acids

41.16

Weight (kDa)

4.81

Isoelectric Point (pI)

44.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ATG5_UblA	PF20638	9 - 102	1.8e-28	Autophagy protein ATG5, UblA domain
ATG5_HBR	PF20637	116 - 172	1e-23	Autophagy protein ATG5, alpha-helical bundle region
ATG5_UblB	PF04106	207 - 356	4e-19	Autophagy protein ATG5, UblB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G17290
fragaria_vesca	FvH4_5g28670 FvH4_5g28670
malus_domestica	MD08G1242300.v1.1 MD15G1436000.v1.1
prunus_persica	Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1 Prupe.1G579800_v2.0.a1
pyrus_communis	pycom08g21020 pycom15g38480
rosa_chinensis	RchiOBHm_Chr2g0106631 RchiOBHm_Chr5g0080521 RchiOBHm_Chr7g0220631
rosa_laevigata	RLG00000002214 RLG00000017523 RLG00000036864
rosa_multiflora	Rmu_co8045066.1_g000001 Rmu_sc0006221.1_g000004 Rmu_sc0006221.1_g000005 Rmu_ssc0000189.1_g000016
rosa_roxburghii	Rroxscaffold_1G00002880 Rroxscaffold_2G00136950 Rroxscaffold_3G00239270
rosa_rugosa	Rorug02G0136800 Rorug05G0469500 Rorug07G0195300
rosa_samantha	Rh2AG188100 Rh2BG199100 Rh2CG193200 Rh2DG194300 Rh5BG545000 Rh5BG545100 Rh5CG568400 Rh5DG555300 Rh7AG336500 Rh7BG327400 Rh7CG354300 Rh7DG332800
rosa_wichuraiana	Rw0G023170 Rw2G014770 Rw7G028690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	106
AatII	GACGTC	1 cut(s)	75
Acc36I	ACCTGC	2 cut(s)	106, 605
AciI	CCGC	1 cut(s)	33
AclWI	GGATC	1 cut(s)	811
AcsI	RAATTY	1 cut(s)	550
AcuI	CTGAAG	3 cut(s)	148, 843, 891
AcyI	GRCGYC	1 cut(s)	72
AfaI	GTAC	2 cut(s)	209, 1080
AfiI	CCNNNNNNNGG	6 cut(s)	122, 228, 341, 495, 581, 839
AflII	CTTAAG	1 cut(s)	512
AflIII	ACRYGT	2 cut(s)	429, 1095
AgsI	TTSAA	3 cut(s)	226, 382, 1075
AhlI	ACTAGT	1 cut(s)	970
AluBI	AGCT	5 cut(s)	113, 168, 366, 454, 517
AluI	AGCT	5 cut(s)	113, 168, 366, 454, 517
Alw21I	GWGCWC	1 cut(s)	1050
Alw26I	GTCTC	2 cut(s)	438, 712
AlwI	GGATC	1 cut(s)	811
Ama87I	CYCGRG	1 cut(s)	1043
AoxI	GGCC	2 cut(s)	141, 722
ApeKI	GCWGC	2 cut(s)	389, 542
ApoI	RAATTY	1 cut(s)	550
AsuC2I	CCSGG	2 cut(s)	318, 497
AsuHPI	GGTGA	3 cut(s)	356, 822, 854
AvaI	CYCGRG	1 cut(s)	1043
BbsI	GAAGAC	1 cut(s)	540
Bbv12I	GWGCWC	1 cut(s)	1050
BbvI	GCAGC	2 cut(s)	376, 554
BccI	CCATC	1 cut(s)	335
BciVI	GTATCC	1 cut(s)	989
BcnI	CCSGG	2 cut(s)	318, 497
BcoDI	GTCTC	2 cut(s)	438, 712
BcuI	ACTAGT	1 cut(s)	970
BfaI	CTAG	1 cut(s)	971
BfmI	CTRYAG	1 cut(s)	47
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	2 cut(s)	106, 605
BfuI	GTATCC	1 cut(s)	989
BisI	GCNGC	2 cut(s)	390, 543
BlsI	GCNGC	2 cut(s)	391, 544
Bme1390I	CCNGG	2 cut(s)	318, 497
BmeT110I	CYCGRG	1 cut(s)	1043
BmiI	GGNNCC	1 cut(s)	898
BmrFI	CCNGG	2 cut(s)	318, 497
BmrI	ACTGGG	1 cut(s)	547
BmsI	GCATC	5 cut(s)	667, 743, 760, 865, 946
BmuI	ACTGGG	1 cut(s)	547
BpiI	GAAGAC	1 cut(s)	540
BpuMI	CCSGG	2 cut(s)	318, 497
BsaHI	GRCGYC	1 cut(s)	72
BsaJI	CCNNGG	4 cut(s)	116, 284, 317, 982
BsaXI	ACNNNNNCTCC	2 cut(s)	637, 667
Bsc4I	CCNNNNNNNGG	6 cut(s)	122, 228, 341, 495, 581, 839
Bse118I	RCCGGY	1 cut(s)	720
Bse1I	ACTGG	2 cut(s)	542, 626
BseDI	CCNNGG	4 cut(s)	116, 284, 317, 982
BseGI	GGATG	1 cut(s)	856
BseLI	CCNNNNNNNGG	6 cut(s)	122, 228, 341, 495, 581, 839
BseMII	CTCAG	2 cut(s)	449, 698
BseNI	ACTGG	2 cut(s)	542, 626
BseRI	GAGGAG	5 cut(s)	105, 171, 756, 759, 860
BseXI	GCAGC	2 cut(s)	376, 554
BsgI	GTGCAG	2 cut(s)	156, 288
BshFI	GGCC	2 cut(s)	143, 724
BsiHKAI	GWGCWC	1 cut(s)	1050
BsiHKCI	CYCGRG	1 cut(s)	1043
BsiSI	CCGG	3 cut(s)	317, 496, 721
BslFI	GGGAC	1 cut(s)	713
BslI	CCNNNNNNNGG	6 cut(s)	122, 228, 341, 495, 581, 839
BsmAI	GTCTC	2 cut(s)	438, 712
BsmFI	GGGAC	1 cut(s)	713
BsnI	GGCC	2 cut(s)	143, 724
BsoBI	CYCGRG	1 cut(s)	1043
Bsp1286I	GDGCHC	1 cut(s)	1050
Bsp143I	GATC	4 cut(s)	105, 256, 442, 816
BspACI	CCGC	1 cut(s)	33
BspANI	GGCC	2 cut(s)	143, 724
BspCNI	CTCAG	2 cut(s)	448, 697
BspLI	GGNNCC	1 cut(s)	898
BspMAI	CTGCAG	1 cut(s)	51
BspMI	ACCTGC	2 cut(s)	106, 605
BspPI	GGATC	1 cut(s)	811
BspTI	CTTAAG	1 cut(s)	512
BsrFI	RCCGGY	1 cut(s)	720
BsrI	ACTGG	2 cut(s)	542, 626
BssAI	RCCGGY	1 cut(s)	720
BssECI	CCNNGG	4 cut(s)	116, 284, 317, 982
BssMI	GATC	4 cut(s)	105, 256, 442, 816
BssNI	GRCGYC	1 cut(s)	72
BssT1I	CCWWGG	2 cut(s)	284, 982
Bst4CI	ACNGT	4 cut(s)	298, 413, 526, 838
Bst6I	CTCTTC	2 cut(s)	87, 897
BstACI	GRCGYC	1 cut(s)	72
BstAFI	CTTAAG	1 cut(s)	512
BstC8I	GCNNGC	1 cut(s)	218
BstDEI	CTNAG	4 cut(s)	109, 187, 435, 684
BstF5I	GGATG	1 cut(s)	856
BstKTI	GATC	4 cut(s)	108, 259, 445, 819
BstMAI	GTCTC	2 cut(s)	438, 712
BstMBI	GATC	4 cut(s)	105, 256, 442, 816
BstNSI	RCATGY	2 cut(s)	433, 1099
BstSCI	CCNGG	2 cut(s)	316, 495
BstSFI	CTRYAG	1 cut(s)	47
BstV1I	GCAGC	2 cut(s)	376, 554
BstV2I	GAAGAC	1 cut(s)	540
BsuI	GTATCC	1 cut(s)	989
BsuRI	GGCC	2 cut(s)	143, 724
BtsCI	GGATG	1 cut(s)	856
BtsIMutI	CAGTG	1 cut(s)	658
BveI	ACCTGC	2 cut(s)	106, 605
Cac8I	GCNNGC	1 cut(s)	218
Cfr10I	RCCGGY	1 cut(s)	720
Csp6I	GTAC	2 cut(s)	208, 1079
CviAII	CATG	5 cut(s)	62, 430, 555, 814, 1096
CviQI	GTAC	2 cut(s)	208, 1079
DdeI	CTNAG	4 cut(s)	109, 187, 435, 684
DpnI	GATC	4 cut(s)	107, 258, 444, 818
DpnII	GATC	4 cut(s)	105, 256, 442, 816
Eam1104I	CTCTTC	2 cut(s)	87, 897
EarI	CTCTTC	2 cut(s)	87, 897
EciI	GGCGGA	1 cut(s)	48
Eco130I	CCWWGG	2 cut(s)	284, 982
Eco32I	GATATC	1 cut(s)	314
Eco57I	CTGAAG	3 cut(s)	148, 843, 891
Eco88I	CYCGRG	1 cut(s)	1043
EcoRI	GAATTC	1 cut(s)	550
EcoRV	GATATC	1 cut(s)	314
EcoT14I	CCWWGG	2 cut(s)	284, 982
ErhI	CCWWGG	2 cut(s)	284, 982
FaeI	CATG	5 cut(s)	65, 433, 558, 817, 1099
FalI	AAGNNNNNCTT	2 cut(s)	350, 382
FaqI	GGGAC	1 cut(s)	713
FatI	CATG	5 cut(s)	61, 429, 554, 813, 1095
Fnu4HI	GCNGC	2 cut(s)	390, 543
FokI	GGATG	1 cut(s)	863
Fsp4HI	GCNGC	2 cut(s)	390, 543
FspBI	CTAG	1 cut(s)	971
GluI	GCNGC	2 cut(s)	390, 543
HaeIII	GGCC	2 cut(s)	143, 724
HapII	CCGG	3 cut(s)	317, 496, 721
Hin1I	GRCGYC	1 cut(s)	72
Hin1II	CATG	5 cut(s)	65, 433, 558, 817, 1099
HindIII	AAGCTT	1 cut(s)	515
HinfI	GANTC	5 cut(s)	52, 65, 289, 331, 827
HpaII	CCGG	3 cut(s)	317, 496, 721
HphI	GGTGA	3 cut(s)	356, 822, 854
Hpy188I	TCNGA	4 cut(s)	70, 120, 687, 871
Hpy188III	TCNNGA	5 cut(s)	570, 600, 778, 803, 883
Hpy99I	CGWCG	2 cut(s)	74, 77
HpyAV	CCTTC	1 cut(s)	604
HpyCH4III	ACNGT	4 cut(s)	298, 413, 526, 838
HpyCH4IV	ACGT	1 cut(s)	72
HpyCH4V	TGCA	9 cut(s)	49, 173, 269, 301, 392, 680, 773, 878, 942
HpyF3I	CTNAG	4 cut(s)	109, 187, 435, 684
HpySE526I	ACGT	1 cut(s)	72
Hsp92I	GRCGYC	1 cut(s)	72
Hsp92II	CATG	5 cut(s)	65, 433, 558, 817, 1099
Kzo9I	GATC	4 cut(s)	105, 256, 442, 816
LmnI	GCTCC	6 cut(s)	35, 94, 118, 363, 451, 896
Lsp1109I	GCAGC	2 cut(s)	376, 554
LweI	GCATC	5 cut(s)	667, 743, 760, 865, 946
MaeI	CTAG	1 cut(s)	971
MaeII	ACGT	1 cut(s)	72
MalI	GATC	4 cut(s)	107, 258, 444, 818
MboI	GATC	4 cut(s)	105, 256, 442, 816
MhlI	GDGCHC	1 cut(s)	1050
MluCI	AATT	5 cut(s)	39, 373, 478, 550, 998
MmeI	TCCRAC	1 cut(s)	93
MseI	TTAA	3 cut(s)	470, 513, 717
MslI	CAYNNNNRTG	2 cut(s)	60, 1062
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	3 cut(s)	317, 496, 721
MspR9I	CCNGG	2 cut(s)	318, 497
NciI	CCSGG	2 cut(s)	318, 497
NdeII	GATC	4 cut(s)	105, 256, 442, 816
NlaIII	CATG	5 cut(s)	65, 433, 558, 817, 1099
NlaIV	GGNNCC	1 cut(s)	898
NspI	RCATGY	2 cut(s)	433, 1099
PaqCI	CACCTGC	1 cut(s)	106
PciI	ACATGT	2 cut(s)	429, 1095
PfeI	GAWTC	5 cut(s)	52, 65, 289, 331, 827
PflFI	GACNNNGTC	1 cut(s)	704
PkrI	GCNGC	2 cut(s)	391, 544
PscI	ACATGT	2 cut(s)	429, 1095
PspN4I	GGNNCC	1 cut(s)	898
PstI	CTGCAG	1 cut(s)	51
PsyI	GACNNNGTC	1 cut(s)	704
RsaI	GTAC	2 cut(s)	209, 1080
RsaNI	GTAC	2 cut(s)	208, 1079
RseI	CAYNNNNRTG	2 cut(s)	60, 1062
SaqAI	TTAA	3 cut(s)	470, 513, 717
SatI	GCNGC	2 cut(s)	390, 543
Sau3AI	GATC	4 cut(s)	105, 256, 442, 816
ScrFI	CCNGG	2 cut(s)	318, 497
SduI	GDGCHC	1 cut(s)	1050
SfaNI	GCATC	5 cut(s)	667, 743, 760, 865, 946
SfcI	CTRYAG	1 cut(s)	47
SmiMI	CAYNNNNRTG	2 cut(s)	60, 1062
SmlI	CTYRAG	1 cut(s)	512
SmoI	CTYRAG	1 cut(s)	512
SpeI	ACTAGT	1 cut(s)	970
Sse9I	AATT	5 cut(s)	39, 373, 478, 550, 998
SsiI	CCGC	1 cut(s)	33
SspMI	CTAG	1 cut(s)	971
StyD4I	CCNGG	2 cut(s)	316, 495
StyI	CCWWGG	2 cut(s)	284, 982
TaaI	ACNGT	4 cut(s)	298, 413, 526, 838
TaiI	ACGT	1 cut(s)	75
TaqI	TCGA	2 cut(s)	204, 924
TaqII	GACCGA	1 cut(s)	454
TasI	AATT	5 cut(s)	39, 373, 478, 550, 998
TatI	WGTACW	1 cut(s)	207
TfiI	GAWTC	5 cut(s)	52, 65, 289, 331, 827
Tru1I	TTAA	3 cut(s)	470, 513, 717
Tru9I	TTAA	3 cut(s)	470, 513, 717
TscAI	CASTG	1 cut(s)	658
TseI	GCWGC	2 cut(s)	389, 542
TspDTI	ATGAA	6 cut(s)	44, 78, 440, 543, 867, 1046
TspGWI	ACGGA	1 cut(s)	965
TspRI	CASTG	1 cut(s)	658
Tth111I	GACNNNGTC	1 cut(s)	704
Vha464I	CTTAAG	1 cut(s)	512
XapI	RAATTY	1 cut(s)	550
XceI	RCATGY	2 cut(s)	433, 1099
XcmI	CCANNNNNNNNNTGG	2 cut(s)	282, 577
XspI	CTAG	1 cut(s)	971
ZraI	GACGTC	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.