MD09G1070900.v1.1
ERF Family

AP2 ERF and B3 domain-containing transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002443
Physical Location & Seq
Forward (+)
4894365 .. 4894979
615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1070900.v1.1.491

Sequence Viewer

Length: 615 bp
ATGACACCCTACGAACGTGATGTCACTCAGGCTCTTTTCTTGGAGTCCCATTCCATGGCTGAGATCGTTGACATGCTTCGAAAACATTCTTATGCCAACGAGCTGGAGATGTACAAGCATAAGTTATACAATGGTGGTGCCCTAGGCCTTGATGCCGGCGGAAAGAGTATTAAGTGTCACCTTGATTTAATGGACACATGTAGTAGCGAGAGGGAGTTGCTTTTCGAGAAAGTGGCAACACCAAGCGATGTAGGGAGATTGAACTGCATGGTTATAACAAAACAGCATGCCGAGAAGCATGTTCAGGTTCAACTGAGTGTAGGGTTTGGTAGAGGGGTGTTGTTGAAATTTGAGGATGAGGTCGGTAAGGGGTGGAGGTTTGGGTATTGTTATTGGAGAAGTAGTCAGAGTTATGTGTTGAGCAAAGGGTGGATTCGCTTTGTGAAGGAGAAGAAGCTGAAAGCTGGTGATGTTGTGAGGCTTGAGAGATCAGTAGGGGAGGATAAGAAGCTGTTCATTGACTGTAGACCAAGAAACGTCGATGTGTCGAGGATCAGGGAGATGCATGGTTGGGTTGGACAATCTCACCATTCCATCATCTTGAACCGTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2 B3

Protein Analysis

205

Amino Acids

23.45

Weight (kDa)

9.04

Isoelectric Point (pI)

30.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 75 - 178 7.2e-22 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 55
AccI GTMKAC 1 cut(s) 526
AciI CCGC 1 cut(s) 159
AclWI GGATC 1 cut(s) 560
AcsI RAATTY 1 cut(s) 347
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 55
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 4 cut(s) 262, 311, 346, 604
AluBI AGCT 4 cut(s) 103, 457, 464, 511
AluI AGCT 4 cut(s) 103, 457, 464, 511
AlwI GGATC 1 cut(s) 560
AoxI GGCC 1 cut(s) 145
ApoI RAATTY 1 cut(s) 347
ArsI GACNNNNNNTTYG 1 cut(s) 38
Asp700I GAANNNNTTC 2 cut(s) 85, 512
AspA2I CCTAGG 1 cut(s) 142
AsuHPI GGTGA 3 cut(s) 170, 479, 578
AsuII TTCGAA 1 cut(s) 79
AvrII CCTAGG 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 142
BanI GGYRCC 1 cut(s) 137
BccI CCATC 1 cut(s) 602
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 523
BlnI CCTAGG 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 139
BmsI GCATC 2 cut(s) 142, 552
BpmI CTGGAG 1 cut(s) 125
Bpu14I TTCGAA 1 cut(s) 79
BpuEI CTTGAG 1 cut(s) 503
BsaJI CCNNGG 2 cut(s) 54, 142
BsaXI ACNNNNNCTCC 2 cut(s) 388, 418
Bsc4I CCNNNNNNNGG 1 cut(s) 55
Bse118I RCCGGY 1 cut(s) 155
BseDI CCNNGG 2 cut(s) 54, 142
BseGI GGATG 1 cut(s) 361
BseLI CCNNNNNNNGG 1 cut(s) 55
BseMII CTCAG 3 cut(s) 41, 51, 305
BseSI GKGCMC 1 cut(s) 142
BshFI GGCC 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 137
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 31, 594
BslI CCNNNNNNNGG 1 cut(s) 55
BsmFI GGGAC 2 cut(s) 31, 594
BsnI GGCC 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 142
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 3 cut(s) 63, 488, 552
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 1 cut(s) 159
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 3 cut(s) 40, 52, 306
BspLI GGNNCC 1 cut(s) 139
BspPI GGATC 1 cut(s) 560
BspT104I TTCGAA 1 cut(s) 79
BspT107I GGYRCC 1 cut(s) 137
BsrFI RCCGGY 1 cut(s) 155
BsrGI TGTACA 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 2 cut(s) 54, 142
BssMI GATC 3 cut(s) 63, 488, 552
BssT1I CCWWGG 2 cut(s) 54, 142
Bst4CI ACNGT 2 cut(s) 524, 608
BstAUI TGTACA 1 cut(s) 111
BstBI TTCGAA 1 cut(s) 79
BstC8I GCNNGC 2 cut(s) 157, 288
BstDEI CTNAG 3 cut(s) 27, 60, 314
BstDSI CCRYGG 1 cut(s) 54
BstF5I GGATG 1 cut(s) 361
BstKTI GATC 3 cut(s) 66, 491, 555
BstMBI GATC 3 cut(s) 63, 488, 552
BstNSI RCATGY 4 cut(s) 76, 201, 290, 302
BstSFI CTRYAG 1 cut(s) 523
BstSLI GKGCMC 1 cut(s) 142
BstXI CCANNNNNNTGG 1 cut(s) 103
BsuRI GGCC 1 cut(s) 147
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 1 cut(s) 361
Cac8I GCNNGC 2 cut(s) 157, 288
Cfr10I RCCGGY 1 cut(s) 155
Csp6I GTAC 1 cut(s) 112
CviAII CATG 7 cut(s) 55, 73, 198, 268, 287, 299, 566
CviJI RGCY 8 cut(s) 32, 59, 103, 147, 457, 464, 481, 511
CviKI_1 RGCY 8 cut(s) 32, 59, 103, 147, 457, 464, 481, 511
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 3 cut(s) 27, 60, 314
DpnI GATC 3 cut(s) 65, 490, 554
DpnII GATC 3 cut(s) 63, 488, 552
EciI GGCGGA 1 cut(s) 174
Eco130I CCWWGG 2 cut(s) 54, 142
Eco147I AGGCCT 1 cut(s) 147
EcoT14I CCWWGG 2 cut(s) 54, 142
EcoT22I ATGCAT 1 cut(s) 567
ErhI CCWWGG 2 cut(s) 54, 142
FaeI CATG 7 cut(s) 58, 76, 201, 271, 290, 302, 569
FaqI GGGAC 2 cut(s) 31, 594
FatI CATG 7 cut(s) 54, 72, 197, 267, 286, 298, 565
FblI GTMKAC 1 cut(s) 526
FokI GGATG 1 cut(s) 368
FspBI CTAG 1 cut(s) 143
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 1 cut(s) 147
HapII CCGG 1 cut(s) 156
Hin1II CATG 7 cut(s) 58, 76, 201, 271, 290, 302, 569
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 2 cut(s) 44, 433
HpaII CCGG 1 cut(s) 156
HphI GGTGA 3 cut(s) 170, 479, 578
Hpy166II GTNNAC 2 cut(s) 70, 527
Hpy188I TCNGA 1 cut(s) 408
Hpy188III TCNNGA 2 cut(s) 226, 601
Hpy8I GTNNAC 2 cut(s) 70, 527
Hpy99I CGWCG 1 cut(s) 542
HpyAV CCTTC 1 cut(s) 439
HpyCH4III ACNGT 2 cut(s) 524, 608
HpyCH4IV ACGT 2 cut(s) 16, 537
HpyCH4V TGCA 2 cut(s) 267, 565
HpyF3I CTNAG 3 cut(s) 27, 60, 314
HpySE526I ACGT 2 cut(s) 16, 537
Hsp92II CATG 7 cut(s) 58, 76, 201, 271, 290, 302, 569
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 3 cut(s) 63, 488, 552
LpnPI CCDG 6 cut(s) 14, 89, 169, 290, 450, 541
LweI GCATC 2 cut(s) 142, 552
MaeI CTAG 1 cut(s) 143
MaeII ACGT 2 cut(s) 16, 537
MaeIII GTNAC 2 cut(s) 22, 176
MalI GATC 3 cut(s) 65, 490, 554
MboI GATC 3 cut(s) 63, 488, 552
MboII GAAGA 1 cut(s) 463
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 1 cut(s) 347
MlyI GAGTC 1 cut(s) 53
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 8 cut(s) 204, 326, 346, 352, 369, 471, 493, 543
Mph1103I ATGCAT 1 cut(s) 567
MroNI GCCGGC 1 cut(s) 155
MroXI GAANNNNTTC 2 cut(s) 85, 512
MseI TTAA 2 cut(s) 171, 188
MslI CAYNNNNRTG 1 cut(s) 90
MspI CCGG 1 cut(s) 156
NaeI GCCGGC 1 cut(s) 157
NcoI CCATGG 1 cut(s) 54
NdeII GATC 3 cut(s) 63, 488, 552
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 7 cut(s) 58, 76, 201, 271, 290, 302, 569
NlaIV GGNNCC 1 cut(s) 139
NmeAIII GCCGAG 1 cut(s) 316
NmuCI GTSAC 2 cut(s) 22, 176
NsiI ATGCAT 1 cut(s) 567
NspI RCATGY 4 cut(s) 76, 201, 290, 302
NspV TTCGAA 1 cut(s) 79
PaeI GCATGC 1 cut(s) 290
PceI AGGCCT 1 cut(s) 147
PciI ACATGT 1 cut(s) 197
PdiI GCCGGC 1 cut(s) 157
PdmI GAANNNNTTC 2 cut(s) 85, 512
PfeI GAWTC 1 cut(s) 433
PflMI CCANNNNNTGG 1 cut(s) 55
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PscI ACATGT 1 cut(s) 197
PsiI TTATAA 1 cut(s) 275
PspN4I GGNNCC 1 cut(s) 139
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 2 cut(s) 171, 188
Sau3AI GATC 3 cut(s) 63, 488, 552
SchI GAGTC 1 cut(s) 53
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 2 cut(s) 142, 552
SfcI CTRYAG 1 cut(s) 523
SfuI TTCGAA 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
SphI GCATGC 1 cut(s) 290
Sse9I AATT 1 cut(s) 347
SseBI AGGCCT 1 cut(s) 147
SsiI CCGC 1 cut(s) 159
SspMI CTAG 1 cut(s) 143
StuI AGGCCT 1 cut(s) 147
StyI CCWWGG 2 cut(s) 54, 142
TaaI ACNGT 2 cut(s) 524, 608
TaiI ACGT 2 cut(s) 19, 540
TaqI TCGA 4 cut(s) 79, 225, 540, 548
TasI AATT 1 cut(s) 347
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 2 cut(s) 171, 188
Tru9I TTAA 2 cut(s) 171, 188
TseFI GTSAC 2 cut(s) 22, 176
Tsp45I GTSAC 2 cut(s) 22, 176
TspDTI ATGAA 1 cut(s) 505
Van91I CCANNNNNTGG 1 cut(s) 55
XapI RAATTY 1 cut(s) 347
XceI RCATGY 4 cut(s) 76, 201, 290, 302
XmaJI CCTAGG 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 526
XmnI GAANNNNTTC 2 cut(s) 85, 512
XspI CTAG 1 cut(s) 143
Zsp2I ATGCAT 1 cut(s) 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.